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Marc Boudvillain

12
Documents
Identifiants chercheurs

Présentation

- **CNRS Research director** and group Leader at CBM - **Deputy director** of CBM since January 2018. Education --------- - **PhD Thesis** in Biochemistry and Molecular Biophysics, University of Orléans (1996). - **Master of Science** (specialty: Biological Chemistry) from the Universities of Orléans, Paris Descartes, and Paris Sud (joint program, 1992). Professional experience ----------------------- - **Tenured Scientist** at CNRS, CBM since October 1999. Biochemistry of RNA and RNA-protein complexes; Regulation of bacterial transcription; RNA helicase mechanisms - **Postdoctoral Research Scientist** at Columbia University and Howard Hugues Medical Institute (New York, USA). Biochemistry of RNA; Structure, function and catalysis by group II intron ribozymes (1996-1999). - **Graduate Research Scientist** at CBM. Nucleic acid Biochemistry; Inorganic chemistry; Engineering of synthetic Pt(II)-based metalloribozymes (1993-1996).

Publications

emmanuel-margeat
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Cryo-EM structure of transcription termination factor Rho from Mycobacterium tuberculosis reveals bicyclomycin resistance mechanism

Emmanuel Saridakis , Rishi Vishwakarma , Josephine Lai-Kee-Him , Kevin Martin , Isabelle Simon
Communications Biology, 2022, 5 (1), pp.120. ⟨10.1038/s42003-022-03069-6⟩
Article dans une revue hal-03563179v1

A Fluorogenic Assay To Monitor Rho-Dependent Termination of Transcription

Cédric Nadiras , Mildred Delaleau , Annie Schwartz , Emmanuel Margeat , Marc Boudvillain
Biochemistry, 2019, 58 (7), pp.865-874. ⟨10.1021/acs.biochem.8b01216⟩
Article dans une revue hal-02067416v1
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Direct observation of the translocation mechanism of transcription termination factor Rho

V. Gocheva , Antoine Le Gall , M. Boudvillain , E. Margeat , Marcelo Nollmann
Nucleic Acids Research, 2015, 43 (4), pp.2367-2377. ⟨10.1093/nar/gkv085⟩
Article dans une revue hal-01171254v1
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The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved nucleotide analog interference probing (trNAIP).

Emilie Soares , Annie Schwartz , Marcelo Nollmann , Emmanuel Margeat , Marc Boudvillain
Nucleic Acids Research, 2014, 42 (14), pp.9270-9284. ⟨10.1093/nar/gku595⟩
Article dans une revue hal-01180888v1

Analysis of helicase-RNA interactions using nucleotide analog interference mapping.

Annie Schwartz , Makhlouf Rabhi , Emmanuel Margeat , Marc Boudvillain
Methods in Enzymology, 2012, 511, pp.149-69. ⟨10.1016/B978-0-12-396546-2.00007-3⟩
Article dans une revue hal-00726256v1

Mutagenesis-Based Evidence for an Asymmetric Configuration of the Ring-Shaped Transcription Termination Factor Rho

Makhlouf Rabhi , Veronika Gocheva , Frederique Jacquinot , Andrea Lee , Emmanuel Margeat
Journal of Molecular Biology, 2011, 405 (2), pp.497-518. ⟨10.1016/j.jmb.2010.11.006⟩
Article dans une revue hal-00608241v1

Keeping up to speed with the transcription termination factor Rho motor.

Marc Boudvillain , Marcelo Nollmann , Emmanuel Margeat
Transcription, 2010, 1 (2), pp.70-75. ⟨10.4161/trns.1.2.12232⟩
Article dans une revue hal-00591655v1

A stepwise 2 '-hydroxyl activation mechanism for the bacterial transcription termination factor Rho helicase

Annie Schwartz , Makhlouf Rabhi , Frederique Jacquinot , Emmanuel Margeat , A. Rachid Rahmouni
Nature Structural and Molecular Biology, 2009, 16 (12), pp.1309-1316. ⟨10.1038/nsmb.1711⟩
Article dans une revue hal-00525325v1
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Transcription termination factor Rho can displace streptavidin from biotinylated RNA

Annie Schwartz , Emmanuel Margeat , A. Rachid Rahmouni , Marc Boudvillain
Journal of Biological Chemistry, 2007, 282 (43), pp.31469-31476. ⟨10.1074/jbc.M706935200⟩
Article dans une revue hal-00176284v1

Structural basis for resistance of Mycobacterium tuberculosis Rho to bicyclomycin

Emmanuel Saridakis , Rishi Vishwakarma , Joséphine Lai Kee Him , Kevin Martin , Isabelle Simon
Helicases & Nucleic Acid-Based Machines: Structure, Mechanism & Regulation, and Roles in HumanDisease, Jul 2021, Paris, France
Communication dans un congrès hal-03419029v1

Single-molecule analysis of E. coli Rho translocase, Helicase, transcription termination activities, and its interactions with RNA polymerase

R. Vishwakarma , A. Nord , F. Pedaci , M. Boudvillain , E. Margeat
Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France
Poster de conférence hal-02902115v1

Relationships between Rho and RNA polymerase in living bacteria

C. Clerte , O. Manier , J-B. Fiche , M. Boudvillain , L. Bossi
Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France
Poster de conférence hal-02902111v1