Researcher identifiers

Number of documents


Curriculume Vitae - Marc BOUDVILLAIN

  • CNRS Research director and group Leader at CBM

  • Deputy director of CBM since January 2018.


  • PhD Thesis in Biochemistry and Molecular Biophysics, University of Orléans (1996).

  • Master of Science (specialty:  Biological Chemistry) from the Universities of Orléans, Paris Descartes, and Paris Sud (joint program, 1992).

Professional experience

  • Tenured Scientist at CNRS, CBM since October 1999. Biochemistry of RNA and RNA-protein complexes; Regulation of bacterial transcription; RNA helicase mechanisms

  • Postdoctoral Research Scientist at Columbia University and Howard Hugues Medical Institute (New York, USA). Biochemistry of RNA; Structure, function and catalysis by group II intron ribozymes (1996-1999).

  • Graduate Research Scientist at CBM. Nucleic acid Biochemistry; Inorganic chemistry; Engineering of synthetic Pt(II)-based metalloribozymes (1993-1996).


Journal articles39 documents

  • Cédric Nadiras, Mildred Delaleau, Annie Schwartz, Emmanuel Margeat, Marc Boudvillain. A Fluorogenic Assay To Monitor Rho-Dependent Termination of Transcription. Biochemistry, American Chemical Society, 2019, 58 (7), pp.865-874. ⟨10.1021/acs.biochem.8b01216⟩. ⟨hal-02067416⟩
  • Lionello Bossi, Mathilde Ratel, Camille Laurent, Patricia Kerboriou, Andrew Camilli, et al.. NusG prevents transcriptional invasion of H-NS-silenced genes. PLoS Genetics, Public Library of Science, 2019, 15 (10), pp.e1008425. ⟨10.1371/journal.pgen.1008425⟩. ⟨hal-02308418⟩
  • Cédric Nadiras, Eric Eveno, Annie Schwartz, Nara Figueroa-Bossi, Marc Boudvillain. A multivariate prediction model for Rho-dependent termination of transcription. Nucleic Acids Research, Oxford University Press, 2018, 46 (16), pp.8245 - 8260. ⟨10.1093/nar/gky563⟩. ⟨hal-01880575⟩
  • Odil Porrua, Marc Boudvillain, Domenico Libri. Transcription Termination: Variations on Common Themes. Trends in Genetics, Elsevier, 2016, 32 (8), pp.508 - 522. ⟨10.1016/j.tig.2016.05.007⟩. ⟨hal-01407669⟩
  • V. Gocheva, A. Le Gall, M. Boudvillain, E. Margeat, Marcelo Nollmann. Direct observation of the translocation mechanism of transcription termination factor Rho. Nucleic Acids Research, Oxford University Press, 2015, 43 (4), pp.2367-2377. ⟨10.1093/nar/gkv085⟩. ⟨hal-01171254⟩
  • F. d'Heygere, A. Schwartz, F. Coste, B. Castaing, M. Boudvillain. ATP-dependent motor activity of the transcription termination factor Rho from Mycobacterium tuberculosis. Nucleic Acids Research, Oxford University Press, 2015, 43, pp.6099-60111. ⟨10.1093/nar/gkv505⟩. ⟨hal-01171219⟩
  • N. Figueroa-Bossi, A. Schwartz, B. Guillemardet, F. d'Heygere, L. Bossi, et al.. RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination. Genes and Development, Cold Spring Harbor Laboratory Press, 2014, 28 (11), pp.1239-1251. ⟨10.1101/gad.240192.114⟩. ⟨hal-01178971⟩
  • Emilie Soares, Annie Schwartz, Marcelo Nollmann, Emmanuel Margeat, Marc Boudvillain. The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved nucleotide analog interference probing (trNAIP).. Nucleic Acids Research, Oxford University Press, 2014, 42 (14), pp.9270-9284. ⟨hal-01180888⟩
  • Rachid Menouni, Stéphanie Champ, Leon Espinosa, Marc Boudvillain, Mireille Ansaldi. Transcription termination controls prophage maintenance in Escherichia coli genomes.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2013, 110 (35), pp.14414-9. ⟨10.1073/pnas.1303400110⟩. ⟨hal-00874882⟩
  • François d'Heygère, Makhlouf Rabhi, Marc Boudvillain. Phyletic distribution and conservation of the bacterial transcription termination factor Rho.. Microbiology, Microbiology Society, 2013, epub ahead of print. ⟨10.1099/mic.0.067462-0⟩. ⟨hal-00840167⟩
  • Francesca Fiorini, Marc Boudvillain, Hervé Le Hir. Tight intramolecular regulation of the human Upf1 helicase by its N- and C-terminal domains. Nucleic Acids Research, Oxford University Press, 2013, 41, pp.2404-2415. ⟨10.1093/nar/gks1320⟩. ⟨hal-00817448⟩
  • Marc Boudvillain, Nara Figueroa-Bossi, Lionello Bossi. Terminator still moving forward: expanding roles for Rho factor.. Current Opinion in Microbiology, Elsevier, 2013, 16 (2), pp.118-24. ⟨10.1016/j.mib.2012.12.003⟩. ⟨hal-00840158⟩
  • Annie Schwartz, Makhlouf Rabhi, Emmanuel Margeat, Marc Boudvillain. Analysis of helicase-RNA interactions using nucleotide analog interference mapping.. Methods in Enzymology, Elsevier, 2012, 511, pp.149-69. ⟨10.1016/B978-0-12-396546-2.00007-3⟩. ⟨hal-00726256⟩
  • Lionello Bossi, Annie Schwartz, Benoit Guillemardet, Marc Boudvillain, Nara Figueroa-Bossi. A role for Rho-dependent polarity in gene regulation by a noncoding small RNA.. Genes and Development, Cold Spring Harbor Laboratory Press, 2012, 26 (16), pp.1864-1873. ⟨10.1101/gad.195412.112⟩. ⟨hal-00727260⟩
  • Makhlouf Rabhi, Veronika Gocheva, Frederique Jacquinot, Andrea Lee, Emmanuel Margeat, et al.. Mutagenesis-Based Evidence for an Asymmetric Configuration of the Ring-Shaped Transcription Termination Factor Rho. Journal of Molecular Biology, Elsevier, 2011, 405 (2), pp.497-518. ⟨10.1016/j.jmb.2010.11.006⟩. ⟨hal-00608241⟩
  • Makhlouf Rabhi, Olivier Espéli, Annie Schwartz, Bastien Cayrol, a Rachid Rahmouni, et al.. The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators.. EMBO Journal, EMBO Press, 2011, 30 (14), pp.2805-16. ⟨10.1038/emboj.2011.192⟩. ⟨hal-00720713⟩
  • Makhlouf Rabhi, Roman Tuma, Marc Boudvillain. RNA remodeling by hexameric RNA helicases. RNA Biology, Taylor & Francis, 2010, 7 (6 - Sp. Iss. SI), pp.655-666. ⟨10.4161/rna.7.6.13570⟩. ⟨hal-00602496⟩
  • Marc Boudvillain, Marcelo Nollmann, Emmanuel Margeat. Keeping up to speed with the transcription termination factor Rho motor.. Transcription, Taylor & Francis, 2010, 1 (2), pp.70-75. ⟨10.4161/trns.1.2.12232⟩. ⟨hal-00591655⟩
  • Annie Schwartz, Makhlouf Rabhi, Frederique Jacquinot, Emmanuel Margeat, A. Rachid Rahmouni, et al.. A stepwise 2 '-hydroxyl activation mechanism for the bacterial transcription termination factor Rho helicase. Nature Structural and Molecular Biology, Nature Publishing Group, 2009, 16 (12), pp.1309-1316. ⟨10.1038/nsmb.1711⟩. ⟨hal-00525325⟩
  • Annie Schwartz, Céline Walmacq, A. Rachid Rahmouni, Marc Boudvillain. Noncanonical Interactions in the Management of RNA Structural Blocks by the Transcription Termination Rho Helicase. Biochemistry, American Chemical Society, 2007, 46 (33), pp.9366 - 9379. ⟨10.1021/bi700493m⟩. ⟨hal-00166551⟩
  • Annie Schwartz, Emmanuel Margeat, A. Rachid Rahmouni, Marc Boudvillain. Transcription termination factor Rho can displace streptavidin from biotinylated RNA. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2007, 282 (43), pp.31469-31476. ⟨10.1074/jbc.M706935200⟩. ⟨hal-00176284⟩
  • C. Walmacq, A.R. Rahmouni, M. Boudvillain. Testing the steric exclusion model for hexameric helicases: substrate features that alter RNA-DNA unwinding by the transcription termination factor Rho. Biochemistry, American Chemical Society, 2006, 45, pp.5885-5895. ⟨10.1021/bi0600648⟩. ⟨hal-00088941⟩
  • C. Walmacq, A.R. Rahmouni, M. Boudvillain. Influence of substrate composition on the helicase activity of transcription termination factor Rho: reduced processivity of Rho hexamers during unwinding of RNA-DNA hybrid regions. Journal of Molecular Biology, Elsevier, 2004, 342 (2), pp.403-420. ⟨10.1016/j.jmb.2004.07.026⟩. ⟨hal-00113090⟩
  • A. Schwartz, A. Rachid Rahmouni, Marc Boudvillain. The functional anatomy of an intrinsic transcription terminator. EMBO Journal, EMBO Press, 2003, 22 (13), pp.3385 - 3394. ⟨10.1093/emboj/cdg310⟩. ⟨hal-01880611⟩
  • Marc Boudvillain, Annie Schwartz, A. Rachid Rahmouni. Limited Topological Alteration of the T7 RNA Polymerase Active Center at Intrinsic Termination Sites †. Biochemistry, American Chemical Society, 2002, 41 (9), pp.3137-3146. ⟨10.1021/bi011358q⟩. ⟨hal-02075291⟩
  • Kathrin Schmidt, Marc Boudvillain, Annie Schwartz, Gijs van der Marel, Jacques van Boom, et al.. Monofunctionallytrans-Diammine Platinum(II)-Modified Peptide Nucleic Acid Oligomers: A New Generation of Potential Antisense Drugs. Chemistry - A European Journal, Wiley-VCH Verlag, 2002, 8 (24), pp.5566 - 5570. ⟨10.1002/1521-3765(20021216)8:24<5566::AID-CHEM5566>3.0.CO;2-#⟩. ⟨hal-01880614⟩
  • Boudvillain Marc, Rozenn Dalbiès-Tran, Marc Leng, Marc Boudvillain. Oligonucleotides Modified With Transplatin Derivatives: Fast and Efficient Metalloribozymes. Metal-Based Drugs, Hindawi Publishing Corporation, 2001, 8 (1), pp.39 - 45. ⟨10.1155/MBD.2001.39⟩. ⟨hal-01880601⟩
  • Jens Müller, Markus Drumm, Marc Boudvillain, Marc Leng, Einar Sletten, et al.. Parallel-stranded DNA with Hoogsteen base pairing stabilized by a trans-[Pt(NH3)2]2+ cross-link: characterization and conversion into a homodimer and a triplex. Journal of Biological Inorganic Chemistry, Springer Verlag, 2000, 5 (5), pp.603-611. ⟨10.1007/s007750000143⟩. ⟨hal-02075297⟩
  • Marc Boudvillain, Alexandre de Lencastre, Anna Marie Pyle. A tertiary interaction that links active-site domains to the 5′ splice site of a group II intron. Nature, Nature Publishing Group, 2000, 406 (6793), pp.315 - 318. ⟨10.1038/35018589⟩. ⟨hal-01880615⟩
  • Anna M. Pyle, V Chu, E Jankowsky, Marc Boudvillain. Using DNAzymes to cut, process, and map RNA molecules for structural studies or modification.. Methods in Enzymology, Elsevier, 1999, 317, pp.140-6. ⟨hal-02096724⟩
  • Françoise Paquet, Marc Boudvillain, Gérard Lancelot, Marc Leng. NMR solution structure of a DNA dodecamer containing a transplatin interstrand GN7-CN3 cross-link. Nucleic Acids Research, Oxford University Press, 1999, 27 (21), pp.4261-4268. ⟨10.1093/nar/27.21.4261⟩. ⟨hal-02075311⟩
  • Marc Boudvillain, Anna M. Pyle. Defining functional groups, core structural features and inter-domain tertiary contacts essential for group II intron self-splicing: a NAIM analysis. EMBO Journal, EMBO Press, 1998, 17 (23), pp.7091-7104. ⟨10.1093/emboj/17.23.7091⟩. ⟨hal-02096717⟩
  • C. Prevost, M. Boudvillain, P. Beudaert, M. Leng, R. Lavery, et al.. Distortions of the DNA double helix induced by 1,3-trans-diamminedichloroplatinum(II)-intrastrand cross-link: an internal coordinate molecular modeling study.. J Biomol Struct Dyn, 1997, 14, pp.703-714. ⟨hal-00313421⟩
  • Caroline Colombier, Marc Boudvillain, Marc Leng. Interstrand Crosslinking Reaction in Transplatin-Modified Oligo-2‵-O-Methyl Ribonucleotide-RNA Hybrids. Antisense and Nucleic Acid Drug Development, Mary Ann Liebert, 1997, 7 (4), pp.397-402. ⟨10.1089/oli.1.1997.7.397⟩. ⟨hal-02322135⟩
  • Marc Boudvillain, C. Prevost, P. Beudaert, M. Leng, R. Lavery, et al.. Distortions of the DNA Double Helix Induced by 1,3-trans-Diamminedichloroplatinum(II)-intrastrand Cross-link: An Internal Coordinate Molecular Modeling Study. Journal of Biomolecular Structure and Dynamics, Taylor & Francis: STM, Behavioural Science and Public Health Titles, 1997, 14 (6), pp.703 - 714. ⟨10.1080/07391102.1997.10508173⟩. ⟨hal-01880624⟩
  • Eloy Bernal-Méndez, Marc Boudvillain, Francisco González-Vílchez, Marc Leng. Chemical Versatility of Transplatin Monofunctional Adducts within Multiple Site-Specifically Platinated DNA †. Biochemistry, American Chemical Society, 1997, 36 (24), pp.7281 - 7287. ⟨10.1021/bi9703148⟩. ⟨hal-01880622⟩
  • Marc Boudvillain, Martine Guerin, Rozenn Dalbiès, Tula Saison-Behmoaras, Marc Leng. Transplatin-Modified Oligo(2‘- O -methyl ribonucleotide)s: A New Tool for Selective Modulation of Gene Expression †. Biochemistry, American Chemical Society, 1997, 36 (10), pp.2925 - 2931. ⟨10.1021/bi962695f⟩. ⟨hal-01880625⟩
  • Marc Boudvillain, R Dalbiès, M. Leng. Evidences for a catalytic activity of the DNA double helix in the reaction between DNA, platinum(II), and intercalators.. Metal Ions in Biological Systems, Marcel Dekker, 1996, 33, pp.87-104. ⟨hal-02322086⟩
  • Marc Boudvillain, Rozenn Dalbiès, Christophe Aussourd, Marc Leng. Intrastrand cross-links are not formed in the reaction between transplatin and native DNA: relation with the clinical inefficency of transplatin. Nucleic Acids Research, Oxford University Press, 1995, 23 (13), pp.2381-2388. ⟨10.1093/nar/23.13.2381⟩. ⟨hal-02075318⟩

Book sections7 documents

  • Cédric Nadiras, Annie Schwartz, Mildred Delaleau, Marc Boudvillain. Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-Dependent Termination of Transcription In Vitro.. Methods in Mecular Biology, 1737, humana press, Totowa, NJ-USA, pp.99-118, 2018, Bacterial regulatory RNA (Arluison & Valverde, eds), ⟨10.1007/978-1-4939-7634-8_7⟩. ⟨hal-01880631⟩
  • F. d'Heygère, A. Schwartz, F. Coste, B. Castaing, M. Boudvillain. Monitoring RNA Unwinding by the Transcription Termination Factor Rho from Mycobacterium tuberculosis. Methods in Mecular Biology, 1259, Springer, pp.293-311, 2015, RNA remodelling proteins (Boudvillain, ed), 978-1-4939-2213-0. ⟨10.1007/978-1-4939-2214-7_18⟩. ⟨hal-01174055⟩
  • Olga Fedorova, Marc Boudvillain, Christina Waldsich. Nucleotide Analog Interference Mapping and Suppression (NAIM/NAIS): a Combinatorial Approach to Study RNA Structure, Folding, and Interaction with Proteins. Handbook of RNA Biochemistry, 2nd edition (Westhof, Bindereif, Schon & Hartmann, eds), Wiley-VCH Verlag GmbH & Co. KGaA, pp.329-368, 2014, ⟨10.1002/9783527647064.ch17⟩. ⟨hal-02097735⟩
  • Makhlouf Rabhi, Rahmouni Rachid A., Marc Boudvillain. Transcription termination factor Rho: a ring-shaped RNA helicase from bacteria. Eckhard Jankowsky. RNA helicases, Royal Society of Chemistry Publishing, pp.243-271, 2010, ⟨10.1039/9781849732215-00243⟩. ⟨hal-00610004⟩
  • Marc Boudvillain, Céline Walmacq, Annie Schwartz, Frédérique Jacquinot. Simple enzymatic assays for the in vitro motor activity of transcription termination factor Rho from Escherichia coli. Mohamed M. M. Abdelhaleem. Methods in Mecular Biology, Humana Press, pp.137-154, 2009, Helicases (Abdelhaleem, ed), ⟨10.1007/978-1-60327-355-8_10⟩. ⟨hal-00623571⟩
  • O. Fedorova, M. Boudvillain, J. Kawaola, A.M. Pyle. Nucleotide analog interference mapping and suppression (NAIM/NAIS): specific applications in studies of RNA tertiary structure, dynamic helicase mechanism, and RNA-protrein interactions. E. Westhof, A. Bindereif, A. Schon, R.I. Hartmann. Handbook of RNA biochemistry, Wiley-VCH, pp.259-293, 2005. ⟨hal-00088591⟩
  • Marc Boudvillain, Dalbiès R., Leng M.. Trans-Diamminedichloroplatinum (II) Is Not an Antitumor Drug: Why?. Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy, 2, Plenum Press, New'Tork, USA, pp.17-25, 1996. ⟨hal-02097755⟩

Patents1 document

  • Marc Leng, Rozenn Dalbiès, Marc Boudvillain. Procédé de pontage sélectif et irréversible d'oligonucléotides platinés sur de l'ARN et ses applications. France, Patent n° : WO1998018915A2. 1996. ⟨hal-02097839⟩