Mots-clés

Co-auteurs

Nombre de documents

52

Propriétés interfaciales des protéines du lait du blanc d'oeuf: une approche 2D-3D


Mes activités concernent les propriétés interfaciales des protéines à l'interface liquide-air: moteurs de l'adsorption, déterminants biochimiques des propriétés interfaciales? Ce travail est mené en commun avec l'UMR INRA-agrocampus 1253,  Science et Technologie du Lait et de l'Oeuf.Ces questions sont articulées depuis peu avec une approche en volume: nous établissons les équations d'état des protéines (diagramme Pi/fraction volumique) par compression osmotique ce qui fournit des informations sur la nature (attractive ou répulsive) des protéines en fonction des conditions physicochimiques.


Article dans une revue23 documents

  • Qingyue Li, Véronique Vié, Hervé Lhermite, Etienne Gaviot, Claire Bourlieu-Lacanal, et al.. Polymer resonators sensors for detection of sphingolipid gel/fluid phase transition and melting temperature measurement. Sensors and Actuators A: Physical , Elsevier, 2017, 263, pp.707-717. 〈10.1016/j.sna.2017.07.037〉. 〈hal-01572203〉
  • C. Pasquier, Sylvie Beaufils, A. Bouchoux, S. Rigault, B. Cabane, et al.. Osmotic pressures of lysozyme solutions from gas-like to crystal states. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2016, 18, pp.28458--28465. 〈10.1039/c6cp03867k〉. 〈hal-01397941〉
  • Cécile Le Floch-Fouéré, Stephane Pezennec, Maryvonne Pasco, Gilles Paboeuf, Anne Renault, et al.. Moderate conformational impact of citrate on ovotransferrin considerably increases its capacity to self-assemble at the interface. Journal of Colloid and Interface Science, Elsevier, 2015, 437, pp.219-226. 〈10.1016/j.jcis.2014.09.035〉. 〈hal-01128555〉
  • Deepti Gupta, Sylvie Beaufils, Véronique Vié, Gilles Paboeuf, Bill Broadhurst, et al.. Crystal structure, biochemical and biophysical characterisation of NHR1 domain of E3 Ubiquitin ligase neutralized. Advances in enzyme research, 2013, 1 (3), pp.61-75. 〈10.4236/aer.2013.13007〉. 〈hal-00939236〉
  • Anne Renault, Jean-François Rioux-Dubé, Sylvie Beaufils, Thierry Lefèvre, Véronique Vié, et al.. Structure and mechanical properties of spider silk films at the air-water interface.. Langmuir, American Chemical Society, 2013, 29 (25), pp.7931-8. 〈10.1021/la401104m〉. 〈hal-00868500〉
  • Nolwenn Huby, Véronique Vié, Anne Renault, Sylvie Beaufils, Thierry Lefèvre, et al.. Native spider silk as a biological optical fiber.. Applied Physics Letters, American Institute of Physics, 2013, 102 (12), pp.123702. 〈10.1063/1.4798552〉. 〈hal-00820827〉
  • David Teboul, Sylvie Beaufils, Jean-Christophe Taveau, Soria Iatmanen-Harbi, Anne Renault, et al.. Mouse TSPO in a lipid environment interacting with a functionalized monolayer.. Biochimica et Biophysica Acta:Biomembranes, Elsevier, 2012, 1818 (11), pp.2791-800. 〈10.1016/j.bbamem.2012.06.020〉. 〈hal-00732807〉
  • Lionel Chièze, Victor Martin Bolanos-Garcia, Gérard Le Caër, Anne Renault, Véronique Vié, et al.. Difference in lipid packing sensitivity of exchangeable apolipoproteins apoA-I and apoA-II: An important determinant for their distinctive role in lipid metabolism.. Biochimica et Biophysica Acta:Biomembranes, Elsevier, 2012, 1818 (11), pp.2732-41. 〈10.1016/j.bbamem.2012.05.016〉. 〈hal-00732810〉
  • Lionel Chièze, Victor Martin Bolanos-Garcia, Mathieu Pinot, Bernard Desbat, Anne Renault, et al.. Fluid and condensed ApoA-I/phospholipid monolayers provide insights into ApoA-I membrane insertion.. Journal of Molecular Biology, Elsevier, 2011, 410 (1), pp.60-76. 〈10.1016/j.jmb.2011.04.006〉. 〈hal-00711936〉
  • Yann Desfougères, Arnaud Saint-Jalmes, Anniina Salonen, Véronique Vié, Sylvie Beaufils, et al.. Strong improvement of interfacial properties can result from slight structural modifications of proteins: the case of native and dry-heated lysozyme.. Langmuir, American Chemical Society, 2011, 27 (24), pp.14947-57. 〈10.1021/la203485y〉. 〈hal-00685099〉
  • Cécile Le Floch-Fouéré, Stéphane Pezennec, Michel Pézolet, Jean-François Rioux-Dubé, Anne Renault, et al.. Unexpected differences in the behavior of ovotransferrin at the air-water interface at pH 6.5 and 8.0.. Journal of Colloid and Interface Science, Elsevier, 2011, 356 (2), pp.614-23. 〈10.1016/j.jcis.2011.01.073〉. 〈hal-00711895〉
  • Semin Lee, Philippe Thebault, Luca Freschi, Sylvie Beaufils, Tom L Blundell, et al.. Characterization of spindle checkpoint kinase Mps1 reveals domain with functional and structural similarities to tetratricopeptide repeat motifs of Bub1 and BubR1 checkpoint kinases.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2011, 287 (8), pp.5988-6001. 〈10.1074/jbc.M111.307355〉. 〈hal-00685033〉
  • Cécile Le Floch-Fouéré, Sylvie Beaufils, Valérie Lechevalier-Datin, Françoise Nau, Michel Pézolet, et al.. Sequential adsorption of egg-white proteins at the air-water interface suggests a stratified organization of the interfacial film. Food Hydrocolloids, Elsevier, 2010, 24 (4), pp.275-284. 〈10.1016/j.foodhyd.2009.10.006〉. 〈hal-00657917〉
  • Anne Renault, Jean-François Rioux-Dubé, Thierry Lefèvre, Stéphane Pezennec, Sylvie Beaufils, et al.. Surface properties and conformation of Nephila clavipes spider recombinant silk proteins at the air-water interface.. Langmuir, American Chemical Society, 2009, 25 (14), pp.8170-80. 〈10.1021/la900475q〉. 〈hal-00662876〉
  • Cécile Le Floch-Fouéré, Stéphane Pezennec, Valérie Lechevalier-Datin, Sylvie Beaufils, Bernard Desbat, et al.. Synergy between ovalbumin and lysozyme leads to non-additive interfacial and foaming properties of mixtures. Food Hydrocolloids, Elsevier, 2009, 23 (2), pp.352-365. 〈10.1016/j.foodhyd.2008.01.007〉. 〈hal-00663172〉
  • Victor Martin Bolanos-Garcia, Anne Renault, Sylvie Beaufils. Surface rheology and adsorption kinetics reveal the relative amphiphilicity, interfacial activity, and stability of human exchangeable apolipoproteins.. Biophysical Journal, Biophysical Society, 2008, 94 (5), pp.1735-45. 〈10.1529/biophysj.107.115220〉. 〈hal-00672641〉
  • Sylvie Beaufils, Günter J. Grossmann, Anne Renault, Victor M. Bolanos-Garcia. Characterization of the Tetratricopeptide-Containing Domain of BUB1, BUBR1, and PP5 Proves That Domain Amphiphilicity over Amino Acid Sequence Specificity Governs Protein Adsorption and Interfacial Activity. Journal of Physical Chemistry B, American Chemical Society, 2008, 112 (27), pp.7984-7991. 〈10.1021/jp711222s〉. 〈hal-00672572〉
  • Sylvie Beaufils, J. Günter Grossmann, Anne Renault, Victor Bolanos-Garcia. Characterization of the tetratricopeptide-containing domain of BUB1, BUBR1, and PP5 proves that domain amphiphilicity over amino acid sequence specificity governs protein adsorption and interfacial activity.. Journal of Physical Chemistry B, American Chemical Society, 2008, 112 (27), pp.7984-91. 〈10.1021/jp711222s〉. 〈hal-00732818〉
  • Thomas Croguennec, Anne Renault, Sylvie Beaufils, Jean-Jacques Dubois, Stéphane Pezennec. Interfacial properties of heat-treated ovalbumin.. Journal of Colloid and Interface Science, Elsevier, 2007, 315 (2), pp.627-36. 〈10.1016/j.jcis.2007.07.041〉. 〈hal-00907909〉
  • Sylvie Beaufils, Rachida Hadaoui-Hammoutène, Véronique Vié, Guy Miranda, Javier Perez, et al.. Comparative behaviour of goat β and αs1-caseins at the air-water interface and in solution. Food Hydrocolloids, Elsevier, 2007, 21 (8), pp.1330-1343. 〈10.1016/j.foodhyd.2006.10.014〉. 〈hal-00908061〉
  • Vm Bolanos-Garcia, S. Beaufils, Anne Renault, Jg Grossmann, S. Brewerton, et al.. The conserved N-terminal region of the mitotic checkpoint protein BUBR1 is a highly surface active domain. Biophysical Journal, Biophysical Society, 2005, 89, pp.2640. 〈hal-00016725〉
  • Sylvie Beaufils, Laurent Cormier, Monica Bionducci, Claude Ecolivet, Georges Calas, et al.. Medium-range order in alkali metaphosphate glasses and melts investigated by reverse Monte Carlo simulations and diffraction analysis.. Physical Review B : Condensed matter and materials physics, American Physical Society, 2003, 67, pp.104201 (1-7). 〈10.1103/PhysRevB.67.104201〉. 〈hal-00085080〉
  • C. Ecolivet, M. Sougoti, Y. Delugeard, S. Beaufils. High resolution Raman study of the soft mode in the incommensurate bis(4-chlorophenyl)sulfone. Journal de Physique I, EDP Sciences, 1994, 4 (10), pp.1451-1460. 〈10.1051/jp1:1994199〉. 〈jpa-00247004〉

Communication dans un congrès18 documents

  • Eric Gicquel, Gilles Paboeuf, Vincent Burgaud, Anne Renault, Sylvie Beaufils, et al.. Un montage pour caractériser l'adsorption de biomolécules aux interfaces fluides. 7ème Colloque Interdisciplinaire en Instrumentation, Jan 2016, Saint-Nazaire, France. 〈hal-01280185〉
  • Eric Gicquel, Gilles Paboeuf, Vincent Burgaud, Sylvie Beaufils, Véronique Vié. Un montage pour caractériser l'adsorption de biomolécules aux interfaces fluides. c2i- 7ème Colloque Interdisciplinaire en Instrumentation, Jan 2016, Saint-Nazaire, France. 〈hal-01455498〉
  • Nolwenn Huby, Anne Renault, Sylvie Beaufils, Véronique Vié, T. Lefevre, et al.. Pristine spider silk fibers as waveguiding microstructure in free space and in an integrated photonic chip. CLEO/Europe-IQEC, May 2013, Munich, France. 〈10.1109/CLEOE-IQEC.2013.6800954〉. 〈hal-00906181〉
  • Sylvie Beaufils, A Renault, A. Saint-Jalmes, G. Paboeuf, Stephane Pezennec, et al.. How Soft Matter Approaches Bring New Tools For Biotechnology. Cosming 2013 CBB Ingrédients Cosmétiques et Biotechnologies, Jun 2013, Saint Malo, France. 2013. 〈hal-01209456〉
  • Nolwenn Huby, Anne Renault, Sylvie Beaufils, Véronique Vié, M. Pezolet, et al.. Optical propagation and integration of pristine silkworm fibers. Frontiers In Optics - FIO, Oct 2012, Rochester (N.Y.), United States. 〈hal-00935623〉
  • S. Pezennec, Cécile Le Floch-Fouéré, S. Bouhallab, Y. Desfougères, V. Lechevalier, et al.. Striking Associative Properties of Food Proteins : a switch to control bulk properties of dispersed media. POLYMERIX, May 2012, Rennes, France. 〈hal-00936057〉
  • Lionel Chièze, V. Bolanos-Garcia, Gérard Le Caër, Anne Renault, B. Desbat, et al.. Lipid Packing Sensitivity of Exchangeable Apolipoproteins apoA-I and apoA-II. Forum des Microscopies à sondes locales, Mar 2012, Saint-Jacut, France. 〈hal-00936083〉
  • Sylvie Beaufils, S. Pezennec, P. Hamon, A. Renault, S. Bouhallab. The Bulk Association Mechanism Between Lysozyme and Alpha-Lactalbumin Is Enlighted by Interfacial Measurements. 14e Conférence Internationale consacrée aux Films Moléculaires Organisés (ICOMF14), Jul 2012, Paris, France. 〈hal-00936050〉
  • Stephane Pezennec, Cécile Le Floch-Fouéré, Said Bouhallab, Yann Desfougeres, Valérie Lechevalier, et al.. Vers un contrôle des propriétés macroscopiques des milieux dispersés par les propriétés associatives des protéines alimentaires. Polymerix 2012, 5. Colloque Européen : Diversité et perspectives d’applications industrielles des biopolymères, May 2012, Rennes, France. 2012. 〈hal-01209700〉
  • Coralie Pasquier, Sophie Rigault, Antoine Bouchoux, Sylvie Beaufils, Stéphane Pezennec, et al.. Equation d’état et structure de solutions de protéines globulaires hautement concentrés. Journées du Groupement de Recherche GDR 2980 Approches Multi-physiques pour les Colloïdes Concentré AMC2, Oct 2012, Rueil-Malmaison, France. 2012. 〈hal-01209870〉
  • Sylvie Beaufils, V. Vie, S. Pezennec, Cécile Le Floch-Fouéré, S. Bouhallab, et al.. Studies at the liquid-air interface: a powerful toolbox to study interactions between biomolecules. 8th EBSA European Biophysics Congress, Aug 2011, Budapest (HO), France. 40, pp.56-56, 2011. 〈hal-00729401〉
  • S. Pezennec, Cécile Le Floch-Fouéré, Y. Desfougères, Valérie Lechevalier-Datin, Thomas Croguennec, et al.. How the physicochemical and structural properties of globular proteins affect their behavior at the air-solution interface and their foaming properties. Third international conference on biofoams, Sep 2011, Capri (IT), France. non paginé, 2011. 〈hal-00729368〉
  • Y. Desfougères, J. Jardin, V. Vié, Valérie Lechevalier-Datin, S. Pézennec, et al.. Comportement interfacial du lysozyme après traitement thermique à sec. 4e Rencontres Biologie-Physique du Grand Ouest, Jun 2009, Ile de Berder (FR), France. 〈hal-00729978〉
  • Valérie Lechevalier, Yann Desfougères, K. Cheng, Stéphane Pezennec, Arnaud Saint-Jalmes, et al.. Heat treatment of ovalbumin powder: interfacial and foaming properties of the different molecular species generated.. ECIS 2009, Sep 2009, antalya, Turkey. 〈hal-01003068〉
  • Cécile Le Floch-Fouéré, Stéphane Pezennec, Valérie Lechevalier-Datin, Françoise Nau, Anne Renault, et al.. Sequential adsorption of egg-white proteins: relation with physical properties of interfacial film. Food colloids: creating structure, delivering functionality, Apr 2008, Le Mans, France. 〈hal-00678269〉
  • Lionel Chièze, Véronique Vié, Anne Renault, Mathieu Pinot, Victor Martin Bolanos-Garcia, et al.. Etude de l'adsorption de l'apolipoprotéine A1 à l'interface liquide-lipide. Groupe d'Etude des Intéractions Molécules-Membranes, 2008, Mittelwihr, France. 〈hal-00716262〉
  • Lionel Chièze, Véronique Vié, M. Pinot, Anne Renault, Victor Martin Bolanos-Garcia, et al.. Propriétés interfaciales des apolipoprotéines Apo A-I, Apo A-II, Apo C-I, Apo C-II, Apo C-III: points communs et spécificités. Rencontres Biologie-Physique du Grand Ouest, Jun 2007, Batz-sur-mer, France. 〈hal-00909239〉
  • Cécile Le Floch-Fouéré, Stéphane Pezennec, Valérie Lechevalier, Sylvie Beaufils, B. Desbat, et al.. Non-additive interfacial properties of ovalbumin and lysozyme in mixtures as revealed by PM-IRRAS and the study of foams. 4th International Conference on Advanced Vibrational Spectroscopy (ICAVS4), Jun 2007, Corfou, Greece. 〈hal-00909249〉

Poster5 documents

  • Coralie Pasquier, Stephane Pezennec, Antoine Bouchoux, Bernard Cabane, Michel Lund, et al.. Equations d'état de solutions hautement concentrées de lysozyme. 6. Rencontres de Biologie Physique du Grand Ouest, Jun 2014, Le Mans, France. 2014. 〈hal-01209640〉
  • Sylvie Beaufils, Guillaume Briand, Said Bouhallab, Valérie Lechevalier, Coralie Pasquier, et al.. Multicouches interfaciales de solutions de protéines révélatrices des interactions attractives en bulk. 6. Rencontres de Biologie-Physique du Grand Ouest ( RBPGO), Jun 2014, Le Mans, France. 2014. 〈hal-01209699〉
  • Stephane Pezennec, Lay-Theng Lee, Antoine Bouchoux, B. Cabane, Coralie Pasquier, et al.. Importance of water flux through protein interfacial films as revealed by neutron reflectometry on lysozyme and ovalbumin layers. “Neutrons and Food 3”, Jul 2014, Paris, France. 2014. 〈hal-01209673〉
  • Stephane Pezennec, Said Bouhallab, Pascaline Hamon, Anne Renault, Sylvie Beaufils. The bulk association mechanism between lysozyme and α-lactalbumine is revealed by interfacial measurements. 14.International Conference on Organized Molecular Films, Jul 2012, Paris, France. 2012. 〈hal-01209658〉
  • Yann Desfougeres, Julien Jardin, V. Vié, Valérie Lechevalier, Stéphane Pezennec, et al.. Comportement interfacial du lysosyme apres traitement thermique a sec. 4. Rencontres Biologie-Physique du Grand Ouest, Jun 2009, Vannes, France. 2009. 〈hal-01454385〉

Autre publication6 documents

  • C. Pasquier, Sylvie Beaufils, A. Bouchoux, B. Cabane, S. Rigult, et al.. Equation of state and structure of highly concentrated globular protein solutions. In food technology, proteins are classically subject to operations leading to high local concentr.. 2012, n.p. 〈hal-00924769〉
  • A. Renault, Sylvie Beaufils, V. Vié, S. Ameziane, S. Pezennec, et al.. Studies at the Liquid-Air Interface : a Powerful Toolbox to Study Interactions Between Biomolecules. Studies at the Liquid-Air Interface : a Powerful Toolbox to Study Interactions Between Biomolecules. 2011, non paginé. 〈hal-00842534〉
  • F. Casanova, S. Pezennec, Sylvie Beaufils, A. Saint Jalmes, Valérie Lechevalier-Datin. Propriétés interfaciales de l'ovalbumine déphosphorylée. Propriétés interfaciales de l'ovalbumine déphosphorylée. 2011, non paginé. 〈hal-00729387〉
  • A. Bouchebbah, Sylvie Beaufils, S. Bouhallab, V. Vié, Valérie Lechevalier-Datin, et al.. Rheological properties of cysteine-modified Ovalbumin at the air-water interface.. Rheological properties of cysteine-modified Ovalbumin at the air-water interface. 2010, non paginé. 〈hal-00729610〉
  • Valérie Lechevalier-Datin, Y. Desfougères, K. Cheng, S. Pezennec, A. Salonen, et al.. Dry-heating of egg white proteins: how a multiscale approach may help to predict foaming properties from 2D interface measurements.. Dry-heating of egg white proteins: how a multiscale approach may help to predict foaming properti.. 2009, non paginé. 〈hal-00729165〉
  • Yann Desfougères, Valérie Lechevalier-Datin, S. Pézennec, F. Artzner, S. Beaufils, et al.. Structural to functional properties of lysozyme heated at 80°C in the dry state. Structural to fucntional properties of lysozyme heated at 80°C in the dry state. 2008, non paginé. 〈hal-00729807〉