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Keywords

Desiccation tolerance Temperature Escherichia-coli Fourier transform Image analysis Resistance Layer-by-layer Saccharomyces cerevisiae FTIR Viability Water activity Foodborne pathogens Enterobacter-sakazakii Dehydration Cronobacter sakazakii Heat treatment Encapsulation Cultivability Yeast Escherichia coli
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36

Lecturer

Dr Stéphane GUYOT

Lecturer

UMR PAM, team PMB – AgroSup Dijon – Université de Bourgogne Franche-Comté

1, Esplande Erasme 21000 Dijon, France

+33 (0)3 80 77 23 87

stephane.guyot@agrosupdijon.fr or stephane.guyot@u-bourgogne.fr

 

1)   Situation

Nationality: French and Swiss

Date of birth: 23rd August 1977

Marital status: married, 4 children

 

2) Education and Career

 

Education

2002-2007        Ph D, ENSBANA, University of Burgundy (Dijon, France)

2001-2002        Master 2 Research of Food Sciences, University of Burgundy

1999-2000        Master 1 of Cellular and Physiological Biology, University of Burgundy

1998-1999        Bachelor of Science in Cellular and Physiological Biology, University of Burgundy

1995-1997        Technical Degree in Biological and Biochemical Analysis, University of Burgundy

 

Career

2008-présent    Lecturer: Microbiological and Food Processes, AgroSup Dijon (France)

2007-2008        Teacher-Researcher (temporary, 50%), ENSBANA, University of Burgundy

2005-2006        Teacher-Researcher (temporary, 50%), ENSBANA, University of Burgundy

2003-2005        High-education teacher, ENSBANA, University of Burgundy

 

Main field of research: Study of microorganism (pathogenic bacteria, yeasts) stress response to improve food processes (as destruction of undesired microorganisms).

Study of the impact of different parameter (as magnitude, kinetics and holding time) inherent to an environmental perturbation (as heat treatments, drying)

·  Use of wild type and mutant strains (KO strains)

·  Impact on cell components (membranes, proteins, sphingolipids, stress granules): flow cytometry, fluorescent microscopy (confocal, two-photon), Fourier-Transform Infrared Spectroscopy …

 

Responsabilities (most significant) / scientific activities

02/2018                        Coordinator of the national workshop “Fight against pathogenic and alteration flora” (Dijon, France)

01/2016-present            One of the three heads of the International Research Master “Microbiology and Physicochemitry for Food and Wine Processes (MP²)”

01/2017-10/2018           Co deputy then Deputy Head of the “Procédés Microbiologiques et Biotechnologiques” research team (UMR PAM)

04/2016-present            Co-responsible of a transversal research area (UMR PAM)

2012-2015                    Head of the 2 years preparatory classes (integrated in AgroSup Dijon)

2012-2013                    Young doctor expert for AERES (evaluation of doctoral schools in Life Sciences at Paris 6, France)

2008-2017                    Reviewer for international peer-reviewed scientific journals: 13 papers

2014-present                 Examiner in 3 thesis juries

 

2)   Scientific production

 

Web of Sciences

Research Gate

Google Scholar

Number of citations

270

306

400

H

10

11

13

H/n (n = 14)

0.71

0.78

0.92

 

 

 

 

 

 

  • 30 articles published in peer-reviewed international scientific journals
  • International congresses : 7 oral presentations (including 1 invited) + 11 written presentations
  • National congresses : 7 oral presentations  + 12 written presentations
  • Regional congresses : 1 oral presentation + 6 written presentations
  • Local seminar : 2 oral presentations  abroad + 1 oral presentation in France

 

3) Co-supervision

  • Thesis: 2 thesis co-supervised between 2013-2016 (ANR EcoSec 33% and CIFRE: 50%), 1 thesis co-supervised since October 2016 (MESR grant: 50%)
  • Postdoctoral training: 2 postdoctoral researchers supervised (100% each, 2015 and 2016)
  • Master 2 Research: 13 students co-supervised (1x100% and 12 x 50%)

Journal articles35 documents

  • Stéphane Guyot, Laurence Pottier, Lucie Bertheau, Jennifer Dumont, Eminence Dorelle Hondjuila Miokono, et al.. Increased xerotolerance of Saccharomyces cerevisiae during an osmotic pressure ramp over several generations. Microbial Biotechnology, Wiley, 2021, pp.1-17. ⟨10.1111/1751-7915.13789⟩. ⟨hal-03174738⟩
  • Fiona Zoz, Stéphane Guyot, Cosette Grandvalet, Mélanie Ragon, Eric Lesniewska, et al.. Management of Listeria monocytogenes on Surfaces via Relative Air Humidity: Key Role of Cell Envelope. Foods, MDPI, 2021, 10 (9), pp.1-15. ⟨10.3390/foods10092002⟩. ⟨hal-03327279⟩
  • Alexandre Prola, Jordan Blondelle, Aymeline Vandestienne, Jérôme Piquereau, Raphaël Denis, et al.. Cardiolipin content controls mitochondrial coupling and energetic efficiency in muscle. Science Advances , American Association for the Advancement of Science (AAAS), 2021, 7 (1), pp.eabd6322. ⟨10.1126/sciadv.abd6322⟩. ⟨hal-03105418⟩
  • Thanh Dat Nguyen, Stéphane Guyot, Caroline Pénicaud, Stéphanie Passot, Christophe Sandt, et al.. Highlighting Protective Effect of Encapsulation on Yeast Cell Response to Dehydration Using Synchrotron Infrared Microspectroscopy at the Single-Cell Level. Frontiers in Microbiology, Frontiers Media, 2020, 11 (6), pp.1887. ⟨10.3389/fmicb.2020.01887⟩. ⟨hal-02939385⟩
  • Hazel Davey, Stéphane Guyot. Estimation of Microbial Viability Using Flow Cytometry. Current Protocols in Cytometry, Wiley, 2020, 93 (1), pp.1-13. ⟨10.1002/cpcy.72⟩. ⟨hal-02544120⟩
  • Ambroise Marin, Emmanuel Denimal, Lucie Bertheau, Stéphane Guyot, Ludovic Journaux, et al.. Automatic Counting of Intra-Cellular Ribonucleo-Protein Aggregates in Saccharomyces cerevisiae Using a Textural Approach. Microscopy and Microanalysis, Cambridge University Press (CUP), 2019, 25 (1), pp.164-179. ⟨10.1017/S1431927619000084⟩. ⟨hal-02171876⟩
  • Emilie Lang, Stéphane Guyot, Caroline Peltier, Pablo Alvarez-Martin, Jean-Marie Perrier-Cornet, et al.. Cellular Injuries in Cronobacter sakazakii CIP 103183T and Salmonella enterica Exposed to Drying and Subsequent Heat Treatment in Milk Powder. Frontiers in Microbiology, Frontiers Media, 2018, 9, pp.475. ⟨10.3389/fmicb.2018.00475⟩. ⟨hal-01907589⟩
  • Fiona Zoz, Cosette Grandvalet, Emilie Lang, Cyril Laconelli, Patrick Gervais, et al.. Listeria monocytogenes ability to survive desiccation: Influence of serotype, origin, virulence, and genotype. International Journal of Food Microbiology, Elsevier, 2017, 248, pp.82 - 89. ⟨10.1016/j.ijfoodmicro.2017.02.010⟩. ⟨hal-01509875⟩
  • Onrawee Laguerre, L. Lecoq, F. Zoz, Stéphane Guyot, Laurent Beney, et al.. Influence of the air humidity on the drying of a liquid droplet on a solid plate and on bacterial destruction. Journal of Food Engineering, Elsevier, 2017, 212, pp.76-86. ⟨10.1016/j.jfoodeng.2017.05.014⟩. ⟨hal-01558465⟩
  • Émilie Lang, Stéphane Guyot, Pablo Alvarez-Martin, Jean-Marie Perrier-Cornet, Patrick Gervais. Caco-2 Invasion by Cronobacter sakazakii and Salmonella enterica Exposed to Drying and Heat Treatments in Dried State in Milk Powder. Frontiers in Microbiology, Frontiers Media, 2017, 8, ⟨10.3389/fmicb.2017.01893⟩. ⟨hal-01618841⟩
  • Meryam Debbabi, Amira Zarrouk, Maryem Bezine, Wiem Meddeb, Thomas Nury, et al.. Comparison of the effects of major fatty acids present in the Mediterranean diet (oleic acid, docosahexaenoic acid) and in hydrogenated oils (elaidic acid) on 7-ketocholesterol-induced oxiapoptophagy in microglial BV-2 cells. Chemistry and Physics of Lipids, Elsevier, 2017, 207 (Pt B), pp.151-170. ⟨10.1016/j.chemphyslip.2017.04.002⟩. ⟨hal-01912331⟩
  • Emmanuel Denimal, Ambroise Marin, Stéphane Guyot, Ludovic Journaux, Paul Molin. Automatic Biological Cell Counting Using a Modified Gradient Hough Transform. Microscopy and Microanalysis, Cambridge University Press (CUP), 2017, 23 (01), pp.11 - 21. ⟨10.1017/S1431927616012617⟩. ⟨hal-01509941⟩
  • Nicolas Sok, Maria Nikolantonaki, Stéphane Guyot, Thanh Dat Nguyen, Anne-Sophie Viaux, et al.. Design of new sensitive α,β-unsaturated carbonyl 1,8-naphtalimide fluorescent probes for thiol bioimaging. Sensors and Actuators B: Chemical, Elsevier, 2017, 242, pp.865 - 871. ⟨10.1016/j.snb.2016.09.171⟩. ⟨hal-01509840⟩
  • Emilie Lang, Cyril Laconelli, Fiona Zoz, Stéphane Guyot, Pablo Alvarez-Martin, et al.. Drying parameters greatly affect the destruction of Cronobacter sakazakii and Salmonella Typhimurium in standard buffer and milk. Food Microbiology, Elsevier, 2017, 62, pp.82 - 91. ⟨10.1016/j.fm.2016.10.005⟩. ⟨hal-01509615⟩
  • Emilie Lang, Layla Chemlal, Paul Molin, Stéphane Guyot, Pablo Alvarez-Martin, et al.. Modeling the heat inactivation of foodborne pathogens in milk powder : High relevance of the substrate water activity. Food Research International, Elsevier, 2017, 99 (1), pp.577-585. ⟨10.1016/j.foodres.2017.06.028⟩. ⟨hal-01558447⟩
  • T. Nguyen, Stéphane Guyot, C. C. Penicaud, S. Passot, C. Sandt, et al.. Understanding the responses of Saccharomyces cerevisiae yeast strain during dehydration processes using synchrotron infrared spectroscopy. Analyst, Royal Society of Chemistry, 2017, 142 (19), pp.3620 - 3628. ⟨10.1039/c7an00257b⟩. ⟨hal-01616052⟩
  • Valeria Amendola, Greta Bergamaschi, Giacomo Dacarro, Franck Denat, Frederic Boschetti, et al.. An Off-On-Off Fluorescent Sensor for pH Windows Based on the 13aneN4-Zn 2+ System. European Journal of Inorganic Chemistry, Wiley-VCH Verlag, 2016, 2016 (32), pp.5106 - 5113. ⟨10.1002/ejic.201600749⟩. ⟨hal-01492871⟩
  • Mélanie Ragon, Hue Nguyen Thi Minh, Stéphane Guyot, Pauline Loison, Gaëtan Burgaud, et al.. Innovative High Gas Pressure Microscopy Chamber Designed for Biological Cell Observation. Microscopy and Microanalysis, Cambridge University Press (CUP), 2016, 22 (01), pp.63 - 70. ⟨10.1017/S1431927615015639⟩. ⟨hal-01485208⟩
  • Thanh Dat Nguyen, Stéphane Guyot, Christophe Sandt, Caroline Pénicaud, Stéphanie Passot, et al.. Protection of yeast cells in micro-organized shells of natural polyelectrolytes during drying process. STP Pharma Pratiques, 2016, 26 (2), pp.133. ⟨hal-01531646⟩
  • Fiona Zoz, Cyril Laconelli, Émilie Lang, Stéphane Guyot, Cosette Grandvalet, et al.. Control of Relative Air Humidity as a Potential Means to Improve Hygiene on Surfaces: A Preliminary Approach with Listeria monocytogenes. PLoS ONE, Public Library of Science, 2016, 11 (2), ⟨10.1371/journal.pone.0148418⟩. ⟨hal-01466198⟩
  • Emmanuel Denimal, Ambroise Marin, Ludovic Journaux, Stéphane Guyot, Paul Molin. Reliable Detection and Smart Deletion of Malassez Counting Chamber Grid in Microscopic White Light Images for Microbiological Applications. Microscopy and Microanalysis, Cambridge University Press (CUP), 2015, pp.1-7. ⟨10.1017/S1431927615000665⟩. ⟨hal-01164294⟩
  • Stéphane Guyot, Patrick Gervais, Michael Young, Pascale Winckler, Jennifer Dumont, et al.. Surviving the heat: heterogeneity of response in S accharomyces cerevisiae provides insight into thermal damage to the membrane. Environmental Microbiology, Society for Applied Microbiology and Wiley-Blackwell, 2015, 17 (8), pp.2982-2992. ⟨10.1111/1462-2920.12866⟩. ⟨hal-02290604⟩
  • Jordan Blondelle, Yusuke Ohno, Vincent Gache, Stéphane Guyot, Sébastien Storck, et al.. HACD1 , a regulator of membrane composition and fluidity, promotes myoblast fusion and skeletal muscle growth. Journal of molecular cell biology, Oxford UP, 2015, 7 (5), pp.429-440. ⟨10.1093/jmcb/mjv049⟩. ⟨hal-02290631⟩
  • Ambroise Marin, Emmanuel Denimal, Stéphane Guyot, Ludovic Journaux, Paul Molin. A Robust Generic Method for Grid Detection in White Light Microscopy Malassez Blade Images in the Context of Cell Counting. Microscopy and Microanalysis, Cambridge University Press (CUP), 2015, 21 (01), pp.239-248. ⟨10.1017/S1431927614013671⟩. ⟨hal-01114543⟩
  • Thanh Dat Nguyen, Stéphane Guyot, Jeannine Lherminier, Yves Waché, Rémi Saurel, et al.. Protection of living yeast cells by micro-organized shells of natural polyelectrolytes. Process Biochemistry, Elsevier, 2015, 50 (10), pp.1528-1536. ⟨10.1016/j.procbio.2015.06.003⟩. ⟨hal-02290612⟩
  • Charline Allonneau, Eric Olmos, Stéphane Guyot, Eric Ferret, Patrick Gervais, et al.. Hydrodynamic characterization of a new small-scale reactor mixed by a magnetic bar. Biochemical Engineering Journal, Elsevier, 2015, 96, pp.29-37. ⟨10.1016/j.bej.2014.12.005⟩. ⟨hal-01267213⟩
  • Julia Hauck Tiburski, Amauri Rosenthal, Stéphane Guyot, Jean-Marie Perrier-Cornet, Patrick Gervais. Water Distribution in Bacterial Spores: A Key Factor in Heat Resistance. Food Biophysics, Springer Verlag (Germany), 2014, 9 (1), pp.10-19. ⟨10.1007/s11483-013-9312-5⟩. ⟨hal-02290639⟩
  • Stéphane Guyot, Laurence Pottier, Alain Hartmann, Mélanie Ragon, Julia Hauck Tiburski, et al.. Extremely rapid acclimation of Escherichia coli to high temperature over a few generations of a fed-batch culture during slow warming. MicrobiologyOpen, Wiley, 2014, 3 (1), pp.52-63. ⟨10.1002/mbo3.146⟩. ⟨hal-02290963⟩
  • Françoise Le Borgne, Stéphane Guyot, Morgan Logerot, Laurent Beney, Patrick Gervais, et al.. Exploration of Lipid Metabolism in Relation with Plasma Membrane Properties of Duchenne Muscular Dystrophy Cells: Influence of L-Carnitine. PLoS ONE, Public Library of Science, 2012, 7 (11), pp.e49346. ⟨10.1371/journal.pone.0049346⟩. ⟨hal-02291000⟩
  • Stéphane Guyot, Laurence Pottier, Eric Ferret, Laurent Gal, Patrick Gervais. Physiological responses of Escherichia coli exposed to different heat-stress kinetics. Archives of Microbiology, Springer Verlag, 2010, 192 (8), pp. 651 - 661. ⟨10.1007/s00203-010-0597-1⟩. ⟨hal-01789685⟩
  • Anne Vejux, Stéphane Guyot, Thomas Montange, Jean-Marc Riedinger, Edmond Kahn, et al.. Phospholipidosis and down-regulation of the PI3-K/PDK-1/Akt signalling pathway are vitamin E inhibitable events associated with 7-ketocholesterol-induced apoptosis. The Journal of Nutritional Biochemistry, Elsevier, 2009, 20 (1), pp.45-61. ⟨10.1016/j.jnutbio.2007.12.001⟩. ⟨hal-02291044⟩
  • Hue Nguyen Thi Minh, Stéphane Guyot, Jean-Marie Perrier-Cornet, Patrick Gervais. Erratum to: Effect of the osmotic conditions during sporulation on the subsequent resistance of bacterial spores. Applied Microbiology and Biotechnology, Springer Verlag, 2008, 80 (1), pp.107-114. ⟨10.1007/s00253-008-1519-x⟩. ⟨hal-02291056⟩
  • Stéphane Guyot, Eric Ferret, Jean-Baptiste Boehm, Patrick Gervais. Yeast cell inactivation related to local heating induced by low-intensity electric fields with long-duration pulses. International Journal of Food Microbiology, Elsevier, 2007, 113 (2), pp.180-188. ⟨10.1016/j.ijfoodmicro.2006.06.036⟩. ⟨hal-02291104⟩
  • Stéphane Guyot, Eric Ferret, Patrick Gervais. Yeast Survival during Thermal and Osmotic Shocks Is Related to Membrane Phase Change. Journal of Agricultural and Food Chemistry, American Chemical Society, 2006, 54 (22), pp.8450-8455. ⟨10.1021/jf0620158⟩. ⟨hal-02291112⟩
  • Stéphane Guyot, Eric Ferret, Patrick Gervais. Responses of Saccharomyces cerevisiae to thermal stress. Biotechnology and Bioengineering, Wiley, 2005, 92 (4), pp.403-409. ⟨10.1002/bit.20600⟩. ⟨hal-02295180⟩

Book sections1 document

  • Mathias Dupuis, Amélie Aude Berthon, François Blondel, Stéphane Guyot, Charlotte Hallavant, et al.. Les Cruchades, Combronde (63). Julie Charmoillaux; Sébastien Gaime. Les formes de l'habitat rural au Moyen Âge en Limagne septentrionale et Sologne bourbonnaise, CNRS éditions, pp.396-408, 2019, Recherches archéologiques, 978-2-271-12878-2. ⟨hal-03497268⟩