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Keywords

Anions Factor H Hyaluronic acid Complement Fucoidan Glycosaminoglycans Glycosaminoglycan Adsorption Capillary electrophoresis Molecular modeling Heparan sulfate ESIMS Mass spectrometry Ions Chondroitin sulfate Oligosaccharide Heparin Carbohydrates Oligosaccharides Complement system
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Number of documents

73

Régis DANIEL

 

CONTEXTE– Notre recherche a pour objectifs la détermination des propriétés structurales et fonctionnelles des polysaccharides bioactifs glycosaminoglycanes (GAGs), de leur analogues d’origine algale et de leurs mimes chimiques [ANR HomeoGAG, 2022], et la compréhension de leurs mécanismes d’action vis-à-vis de cascades biologiques et de protéines partenaires (immunité innée dans le système du complément humain, chimiokines, inflammation). Le Vivant repose pour son fonctionnement sur les propriétés structurales et fonctionnelles de grandes familles de biopolymères, parmi lesquelles celle des polysaccharides exposés à la surface cellulaire ou constituant des glyco-conjugués avec les protéines reste encore mal connue. Cantonnés aux rôles structural et de soutien, les polysaccharides ont gagné depuis peu leur place dans le concert des acteurs biomoléculaires du Vivant, soulevant toutes une séries de questions sur les bases structurales et les mécanismes moléculaires de leurs propriétés fonctionnelles.

AXE STRUCTURAL– Le progrès des connaissances dans ce domaine nécessite de lever des verrous analytiques en particulier pour la caractérisation structurale et le séquençage de séquences glucidiques bioactives, dont les méthodes font aujourd’hui encore défaut. Nous adressons ces défis par une approche pluridisciplinaire à l’interface de la chimie, de la physique et de la biologie. Nous exploitons en particulier les fortes potentialités de la spectrométrie de masse (MS) dans le domaine de la glycobiologie, et ses couplages avec la mobilité ionique [Rapid Commun. Mass Spectrom. 2017], la chromatographie [HAL] et la spectroscopie UV et infra-rouge [Curr. Opin. Struct. Biol. 2018] afin de décoder le contenu informationnel porté par les séquences bioactives. Cet alphabet à déchiffrer repose des déterminants structuraux – entre autres : distribution de groupes sulfate, anomèrie, épimérisation – conduisant à une syntaxe isomérique d’une complexité considérable. Nous étudions également la lecture de ces séquences à l’échelle de la molécule unique en exploitant les propriétés de confinement de nanopores biologiques [ACS Nano 2012], à l’image des procédés de séquençage par nanopore existant déjà pour les acides nucléiques. Nous montrons que des séquences oligosaccharidiques de GAGs peuvent être analysées et distinguées par cette méthode nanopore [Eur. Phys. J E Soft Matter. 2018].

AXE FONCTIONEL– L’élucidation structurale de séquences glucidiques bioactives est le préalable à l’établissement de relations structure/activité. En particulier, afin de mieux comprendre les mécanismes d’interaction et de formation de complexe non-covalents glucide-protéine, nous développons les couplages entre l’électrophorèse capillaire d’affinité et la MS électrospray (ACE-MS), et entre la résonnance plasmonique de surface par imagerie et la MS MALDI (SPRi-MS) [Antibody Arrays, Methods and Protocols, Springer US 2020], ces deux méthodes étant « label-free » et particulièrement adaptées aux complexes protéine-glucide [Anal. Bioanal. Chem. 2020]. Ces couplages MS permettent d’identifier des séquences glucidiques liantes et de caractériser les complexes non-covalents formés (stœchiométrie, constantes thermodynamiques). Du côté du partenaire protéique, nous étudions les processus d’oligomérisation et de modifications conformationnelles engendrés par la liaison d’une séquence glucidiques. Comme pour le défi d’analyse structural, celui posé par la formation  de ces complexes glyco-protéique non-covalents nécessite des approches innovantes. Celle que nous développons avec le marquage résolu en temps par rayonnement synchrotron et analyse MS permet de cartographier finement la surface protéique exposée au solvant et d’identifier sur la protéine en solution les domaines impliqués dans la liaison du ligand oligosaccharidique [J. Synchr. Rad. 2017].

Nous complétons ce dispositif d’étude fonctionnelle, par la caractérisation d’enzymes agissant sur les oligo/polysaccharides de GAGs telles que les enzymes humaines sulfatases HSulfs [Biochem. Biophys. Rep. 2019] et polysaccharidase hyaluronidase [Glycobiology 2021], et bactérienne CS 4-O-sulfatase [Biochem. J. 2020] qui se révèlent des outils précieux d’exploration structurale et fonctionnelle [ANR Sulf@as, 2022].

L’ensemble de ces travaux s’appuie sur le  riche plateau instrumental du LAMBE comprenant la plate-forme de spectrométrie de masse (7 instruments et ses couplages LC, EC et SPR) labelisée Génopole, les dispositifs nanopore et l’imagerie AFM, ainsi que sur les lignes de lumière METROLOGIE, et DISCO du Synchrotron SOLEIL.

Journal articles68 documents

  • Régis Daniel. OUP accepted manuscript. Glycobiology, Oxford University Press (OUP), In press, ⟨10.1093/glycob/cwab004⟩. ⟨hal-03103354⟩
  • Cédric Przybylski, Florence Gonnet, Els Saesen, Hugues Lortat-Jacob, Régis Daniel. Surface plasmon resonance imaging coupled to on-chip mass spectrometry: a new tool to probe protein-GAG interactions. Analytical and Bioanalytical Chemistry, Springer Verlag, 2020, ⟨10.1007/s00216-019-02267-2⟩. ⟨hal-02401458⟩
  • Wenshuang Wang, Cédric Przybylski, Xiaojuan Cai, Chrystel Lopin-Bon, Runmiao Jiao, et al.. Investigation of Action Pattern of a Novel Chondroitin Sulfate/Dermatan Sulfate 4- O -Endosulfatase. Biochemical Journal, Portland Press, 2020, 478 (2), pp.281-298. ⟨10.1042/BCJ20200657⟩. ⟨hal-03098070⟩
  • Ilham Seffouh, Cédric Przybylski, Amal Seffouh, Rana El Masri, Romain R Vivès, et al.. Mass spectrometry analysis of the human endosulfatase Hsulf-2. Biochemistry and Biophysics Reports, 2019, 18, pp.100617. ⟨10.1016/j.bbrep.2019.01.010⟩. ⟨hal-02043614⟩
  • Aziz Fennouri, Joana Ramiandrisoa, Laurent Bacri, Jérôme Mathé, Régis Daniel. Comparative biosensing of glycosaminoglycan hyaluronic acid oligo- and polysaccharides using aerolysin and α-hemolysin nanopores. European Physical Journal E: Soft matter and biological physics, EDP Sciences: EPJ, 2018, 41 (10), pp.127. ⟨10.1140/epje/i2018-11733-5⟩. ⟨hal-01966717⟩
  • Isabelle Compagnon, Baptiste Schindler, Gina Renois-Predelus, Régis Daniel. Lasers and ion mobility: new additions to the glycosaminoglycanomics toolkit. Current Opinion in Structural Biology, Elsevier, 2018, 50, pp.171-180. ⟨10.1016/j.sbi.2018.06.006⟩. ⟨hal-01966719⟩
  • Nicolas Merle, Anne Grunenwald, Marie-Lucile Figueres, Sophie Chauvet, Marie Daugan, et al.. Characterization of Renal Injury and Inflammation in an Experimental Model of Intravascular Hemolysis. Frontiers in Immunology, Frontiers, 2018, 9, pp.179. ⟨10.3389/fimmu.2018.00179⟩. ⟨hal-01966724⟩
  • Anna Baud, Laure Aymé, Florence Gonnet, Isabelle Salard, Yann Gohon, et al.. SOLEIL shining on the solution-state structure of biomacromolecules by synchrotron X-ray footprinting at the metrology beamline. Journal of Synchrotron Radiation, International Union of Crystallography, 2017, 24, pp.576-585. ⟨10.1107/S1600577517002478⟩. ⟨hal-01608337⟩
  • Baptiste Schindler, Loïc Barnes, C. Gray, S. Chambert, L. Flitsch, et al.. IRMPD Spectroscopy Sheds New (Infrared) Light on the Sulfate Pattern of Carbohydrates. Journal of Physical Chemistry A, American Chemical Society, 2017, 121 (10), pp.2114-2120. ⟨10.1021/acs.jpca.6b11642⟩. ⟨hal-01966732⟩
  • Shambhuprasad Togarsimalemath, Sidharth Sethi, Rajan Duggal, Moglie Le Quintrec, Pranaw Jha, et al.. A novel CFHR1-CFHR5 hybrid leads to a familial dominant C3 glomerulopathy. Kidney International, Nature Publishing Group, 2017, 92 (4), pp.876 - 887. ⟨10.1016/j.kint.2017.04.025⟩. ⟨hal-01843963⟩
  • Salomé Poyer, Chrystel Lopin-Bon, Jean-Claude Jacquinet, Jean-Yves Salpin, Régis Daniel. Isomer separation and effect of the degree of polymerization on the gas-phase structure of chondroitin sulfate oligosaccharides analyzed by ion mobility and tandem mass spectrometry. Rapid Communications in Mass Spectrometry, Wiley, 2017, 31 (23), pp.2003 -2010. ⟨10.1002/rcm.7987⟩. ⟨hal-01637356⟩
  • Vincent Puy, Walaa Darwiche, Pierre-Edouard Bodet, Christelle Lony, Cathy Gomila, et al.. In Sanfilippo syndrome, heparan sulfate hexasaccharides are the most pathogenic fractions involved in glia activation.. Molecular Genetics and Metabolism, Elsevier, 2017, 120 (1-2), pp.S112. ⟨10.1016/J.YMGME.2016.11.287⟩. ⟨hal-02110168⟩
  • Pierre-Edouard Bodet, Isabelle Salard, Cédric Przybylski, Florence Gonnet, Cathy Gomila, et al.. Efficient recovery of glycosaminoglycan oligosaccharides from polyacrylamide gel electrophoresis combined with mass spectrometry analysis. Analytical and Bioanalytical Chemistry, Springer Verlag, 2017, 409 (5), pp.1257-1269. ⟨10.1007/s00216-016-0052-5⟩. ⟨hal-01966743⟩
  • Adeline Bourgeault, Véronique Legros, Florence Gonnet, Régis Daniel, Aurélie Paquirissamy, et al.. Interaction of TiO2 nanoparticles with proteins from aquatic organisms: the case of gill mucus from blue mussel. Environmental Science and Pollution Research, Springer Verlag, 2017, 24 (15), pp.13474-13483. ⟨10.1007/s11356-017-8801-3⟩. ⟨hal-01966733⟩
  • Vincent Puy, Pierre-Edouard Bodet, Camille Rottier, Rémi Delaunay, Cathy Gomila, et al.. Corrigendum to “Alteration of cerebral iron metabolism in Sanfilippo syndrome” [Mol. Genet. Metab. 117 2, February 2016, p. S97]. Molecular Genetics and Metabolism, Elsevier, 2016, 118 (1), pp.64. ⟨10.1016/j.ymgme.2016.03.005⟩. ⟨hal-02065247⟩
  • José Neira, Encarnación Medina-Carmona, José Hernández-Cifre, Laia Montoliu-Gaya, Ana Camara-Artigas, et al.. The chondroitin sulfate/dermatan sulfate 4-O-endosulfatase from marine bacterium Vibrio sp FC509 is a dimeric species: Biophysical characterization of an endosulfatase. Biochimie, Elsevier, 2016, 131, pp.85-95. ⟨10.1016/j.biochi.2016.09.015⟩. ⟨hal-01966753⟩
  • Anna Baud, Florence Gonnet, Isabelle Salard, Maxime Le Mignon, Alexandre Giuliani, et al.. Probing the solution structure of Factor H using hydroxyl radical protein footprinting and cross-linking. Biochemical Journal, Portland Press, 2016, 473 (12), pp.1805-1819. ⟨10.1042/BCJ20160225⟩. ⟨hal-01966773⟩
  • C. Przybylski, G. Correc, M. Fer, F. Gonnet, W. Helbert, et al.. MALDI-TOF MS and ESI-LTQ-Orbitrap tandem mass spectrometry reveal specific porphyranase activity from a Pseudoalteromonas atlantica bacterial extract. RSC Advances, Royal Society of Chemistry, 2015, 5 (98), pp.80793-80803. ⟨10.1039/C5RA14449C⟩. ⟨hal-01966791⟩
  • Johana Musso, William Buchmann, Florence Gonnet, Nathalie Jarroux, Sophie Bellon, et al.. Biomarkers probed in saliva by surface plasmon resonance imaging coupled to matrix-assisted laser desorption/ionization mass spectrometry in array format. Analytical and Bioanalytical Chemistry, Springer Verlag, 2015, 407 (5), pp.1285-1294. ⟨10.1007/s00216-014-8373-8⟩. ⟨hal-01966798⟩
  • Cédric Przybylski, Meriem Mokaddem, Mehdi Prull-Janssen, Els Saesen, Hugues Lortat-Jacob, et al.. On-line capillary isoelectric focusing hyphenated to native electrospray ionization mass spectrometry for the characterization of interferon-γ and variants. Analyst, Royal Society of Chemistry, 2015, 140 (2), pp.543-550. ⟨10.1039/c4an01305k⟩. ⟨hal-01096701⟩
  • Elodie Alinat, Nathalie Delaunay, Cédric Przybylski, Régis Daniel, Xavier Archer, et al.. Capillary electrophoresis fingerprinting of 8-aminopyrene-1,3,6-trisulfonate derivatized nitrocellulose after partial acid depolymerization. Journal of Chromatography A, Elsevier, 2015, 1387, pp.134-143. ⟨10.1016/j.chroma.2015.01.092⟩. ⟨hal-01966794⟩
  • Sonia Korchane, Antoine Pallandre, Cédric Przybylski, Antoine Poüs, Florence Gonnet, et al.. Derivatization strategies for CE-LIF analysis of biomarkers: Toward a clinical diagnostic of familial transthyretin amyloidosis. Electrophoresis, Wiley-VCH Verlag, 2014, 35 (7), pp.1050-1059. ⟨10.1002/elps.201300426⟩. ⟨hal-01966802⟩
  • Stephanie Devineau, Christelle Mathé, Véronique Legros, Florence Gonnet, Régis Daniel, et al.. The nano-bio interface mapped by oxidative footprinting of the adsorption sites of myoglobin. Analytical and Bioanalytical Chemistry, Springer Verlag, 2014, 406, pp.8037 - 8040. ⟨10.1007/s00216-014-8188-7⟩. ⟨cea-01758749⟩
  • Aziz Fennouri, Régis Daniel, Manuela Pastoriza-Gallego, Loïc Auvray, Juan Pelta, et al.. Kinetics of Enzymatic Degradation of High Molecular Weight Polysaccharides through a Nanopore: Experiments and Data-Modeling. Analytical Chemistry, American Chemical Society, 2013, 85 (18), pp.8488-8492. ⟨10.1021/ac4020929⟩. ⟨hal-01967533⟩
  • Anne-Lise Marie, Cédric Przybylski, Florence Gonnet, Régis Daniel, Remi Urbain, et al.. Capillary zone electrophoresis and capillary electrophoresis-mass spectrometry for analyzing qualitative and quantitative variations in therapeutic albumin. Analytica Chimica Acta, Elsevier Masson, 2013, 800, pp.103-110. ⟨10.1016/j.aca.2013.09.023⟩. ⟨hal-01967529⟩
  • Amal Seffouh, Fabian Milz, Cédric Przybylski, Cédric Laguri, Arie Oosterhof, et al.. HSulf sulfatases catalyze processive and oriented 6-O-desulfation of heparan sulfate that differentially regulates fibroblast growth factor activity. FASEB Journal, Federation of American Society of Experimental Biology, 2013, 27 (6), pp.2431-2439. ⟨10.1096/fj.12-226373⟩. ⟨hal-00871189⟩
  • Isabelle Bally, Sarah Ancelet, Christine Moriscot, Florence Gonnet, Alberto Mantovani, et al.. Expression of recombinant human complement C1q allows identification of the C1r/C1s-binding sites.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2013, 110 (21), pp.8650-8655. ⟨10.1073/pnas.1304894110⟩. ⟨hal-01179709⟩
  • Aziz Fennouri, Cédric Przybylski, Manuela Pastoriza-Gallego, Laurent Bacri, Loïc Auvray, et al.. Single Molecule Detection of Glycosaminoglycan Hyaluronic Acid Oligosaccharides and Depolymerization Enzyme Activity Using a Protein Nanopore. ACS Nano, American Chemical Society, 2012, 6 (11), pp.9672-9678. ⟨10.1021/nn3031047⟩. ⟨hal-01967543⟩
  • Cédric Przybylski, Florence Gonnet, William Buchmann, Régis Daniel. Critical parameters for the analysis of anionic oligosaccharides by desorption electrospray ionization mass spectrometry. Journal of Mass Spectrometry, Wiley-Blackwell, 2012, 47 (8), pp.1047-1058. ⟨10.1002/jms.3052⟩. ⟨hal-01967547⟩
  • Isabelle Bally, Sarah Ancelet, Christine Moriscot, Florence Bonnet, Régis Daniel, et al.. Production of functional full-length recombinant human C1q. Immunobiology, Elsevier, 2012, 217 (11), pp.1194. ⟨10.1016/j.imbio.2012.08.187⟩. ⟨hal-02021968⟩
  • Laurent Bacri, Abdelghani Oukhaled, Erven Hémon, Fenseth Banzouzi Bassafoula, Loïc Auvray, et al.. Discrimination of neutral oligosaccharides through a nanopore. Biochemical and Biophysical Research Communications, Elsevier, 2011, 412 (4), pp.561-564. ⟨10.1016/j.bbrc.2011.07.121⟩. ⟨hal-01967554⟩
  • David Ropartz, Pierre-Edouard Bodet, Cédric Przybylski, Florence Gonnet, Régis Daniel, et al.. Performance evaluation on a wide set of matrix-assisted laser desorption ionization matrices for the detection of oligosaccharides in a high-throughput mass spectrometric screening of carbohydrate depolymerizing enzymes. Rapid Communications in Mass Spectrometry, Wiley, 2011, 25 (14), pp.2059-2070. ⟨10.1002/rcm.5060⟩. ⟨hal-01967556⟩
  • Marie-Jeanne Clément, Bérangère Tissot, Lionel Chevolot, Elisabeth Adjadj, Yuguo Du, et al.. NMR characterization and molecular modeling of fucoidan showing the importance of oligosaccharide branching in its anticomplementary activity. Glycobiology, Oxford University Press (OUP), 2010, 20 (7), pp.883-894. ⟨10.1093/glycob/cwq046⟩. ⟨hal-01967562⟩
  • Delphine Pflieger, Cédric Przybylski, Florence Gonnet, Jean-Pierre Le Caer, Thomas Lunardi, et al.. Analysis of Human C1q by Combined Bottom-up and Top-down Mass Spectrometry. Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2010, 9 (4), pp.593-610. ⟨10.1074/mcp.M900350-MCP200⟩. ⟨hal-01967565⟩
  • Cedric Przybylski, Florence Gonnet, David Bonnaffé, Yael Hersant, Hugues Lortat-Jacob, et al.. HABA-based ionic liquid matrices for UV-MALDI-MS analysis of heparin and heparan sulfate oligosaccharides.. Glycobiology, Oxford University Press (OUP), 2010, 20 (2), pp.224-234. ⟨10.1093/glycob/cwp169⟩. ⟨hal-01063049⟩
  • C. Przybylski, F. Gonnet, Y. Hersant, D. Bonnaffé, Hugues Lortat-Jacob, et al.. Desorption Electrospray Ionization Mass Spectrometry of Glycosaminoglycans and Their Protein Noncovalent Complex. Analytical Chemistry, American Chemical Society, 2010, 82 (22), pp.9225-9233. ⟨10.1021/ac1016198⟩. ⟨hal-01967559⟩
  • Sébastien Brier, Delphine Pflieger, Maxime Le Mignon, Isabelle Bally, Christine Gaboriaud, et al.. Mapping surface accessibility of the C1r/C1s tetramer by chemical modification and mass spectrometry provides new insights into assembly of the human C1 complex. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2010, 285 (42), pp.32251-32263. ⟨10.1074/jbc.M110.149112⟩. ⟨hal-01967560⟩
  • S. Bellon, W. Buchmann, F. Gonnet, N. Jarroux, M. Anger-Leroy, et al.. Hyphenation of Surface Plasmon Resonance Imaging to Matrix-Assisted Laser Desorption Ionization Mass Spectrometry by On-Chip Mass Spectrometry and Tandem Mass Spectrometry Analysis. Analytical Chemistry, American Chemical Society, 2009, 81 (18), pp.7695-7702. ⟨10.1021/ac901140m⟩. ⟨hal-01967571⟩
  • Françoise Lavialle, Sophie Deshayes, Florence Gonnet, Eric Larquet, Sergei Kruglik, et al.. Nanovesicles released by Dictyostelium cells: A potential carrier for drug delivery. International Journal of Pharmaceutics, Elsevier, 2009, 380 (1-2), pp.206-215. ⟨10.1016/j.ijpharm.2009.06.039⟩. ⟨hal-01967573⟩
  • Soraya Fermas, Régis Daniel, Florence Gonnet. The polyethylene oxide capillary coating is compatible with capillary electrophoresis–mass spectrometry analysis of basic proteins. Analytical Biochemistry, Elsevier Masson, 2008, 372 (2), pp.258-260. ⟨10.1016/j.ab.2007.09.019⟩. ⟨hal-00271307⟩
  • Soraya Fermas, Florence Gonnet, Angela Sutton, Nathalie Charnaux, Barbara Mulloy, et al.. Sulfated oligosaccharides (heparin and fucoidan) binding and dimerization of stromal cell-derived factor-1 (SDF-1/CXCL 12) are coupled as evidenced by affinity CE-MS analysis. Glycobiology, Oxford University Press (OUP), 2008, 18 (12), pp.1054-1064. ⟨10.1093/glycob/cwn088⟩. ⟨hal-01967643⟩
  • Soraya Fermas, Florence Gonnet, Anne Varenne, Pierre Gareil, Régis Daniel. Frontal analysis capillary electrophoresis hyphenated to electrospray ionization mass spectrometry for the characterization of the antithrombine/heparin pentasaccharide complex. Analytical Chemistry, American Chemical Society, 2007, 79 (13), pp.4987-4993. ⟨10.1021/ac070146h⟩. ⟨hal-00271297⟩
  • Soraya Fermas, Florence Gonnet, Anne Varenne, Pierre Gareil, Régis Daniel. Intérêt du couplage de l'électrophorèse capillaire en mode frontal et de la spectrométrie de masse pour la caractérisation des complexes non-covalents glucide/protéine. Spectra Analysis, 2007, 257, pp.30-35. ⟨hal-00271303⟩
  • Régis Daniel, L. Chevolot, M. Carrascal, B. Tissot, P. Mourão, et al.. Electrospray ionization mass spectrometry of oligosaccharides derived from fucoidan of Ascophyllum nodosum. Carbohydrate Research, Elsevier, 2007, 342 (6), pp.826-834. ⟨10.1016/j.carres.2007.01.009⟩. ⟨hal-00159438⟩
  • Bérangère Tissot, Jean-Yves Salpin, Michael Martinez, Marie-Pierre Gaigeot, Régis Daniel. Differentiation of the fucoidan bulding blocks sulfated l-fucose isomers by electrospray ionization mass spectrometry, hyphenated capillary electrophoresis and theoretical molecular calculations. Carbohydrate Research, Elsevier, 2006, 341 (5), pp.598-609. ⟨10.1016/j.carres.2005.11.029⟩. ⟨hal-00068637⟩
  • Régis Daniel, B. Tissot, Christophe Place, Z. Guéroui. Peigner de l'ADN pour étudier les interactions polysaccharide-protéine. L'Actualité Chimique, Société chimique de France, 2006, 297, pp.15-20. ⟨hal-00157520⟩
  • Bérangère Tissot, Florence Gonnet, Alban Iborra, Christian Berthou, Nicole Thielens, et al.. Mass spectrometry analysis of complement protein C1q in the study of its interaction properties with fucoidan.. Biochemistry, American Chemical Society, 2005, 44, pp.2602-2609. ⟨hal-00015945⟩
  • M.Y. Kim, A. Varenne, Régis Daniel, P. Gareil. Capillary electrophoresis profiles of fucoidan and heparin fractions: significance of mobility dispersity for their characterization. Journal of Separation Science, Wiley-VCH Verlag, 2003, 26(12-13), pp.1154-1162. ⟨10.1016/S0003-2697(02)00687-5⟩. ⟨hal-00163452⟩
  • B. Tissot, B. Montdargent, L. Chevolot, A. Varenne, S. Descroix, et al.. Interaction of fucoidan with the complement proteins of the classical pathway. Biochimica et Biophysica Acta Proteins and Proteomics, Elsevier, 2003, 1651, pp.5-16. ⟨hal-00163432⟩
  • Bérangère Tissot, Régis Daniel, Christophe Place. Interaction of the C1 complex of complement with sulfated polysaccharide and DNA probed by single molecule fluorescence microscopy.. European Journal of Biochemistry, Wiley, 2003, 270 (23), pp.4714-20. ⟨10.1046/j.1432-1033.2003.03870.x⟩. ⟨hal-01967657⟩
  • B. Tissot, Régis Daniel. Letter to the Glyco-Forum: Biological properties of sulfated fucans: the potent inhibiting activity of algal fucoidan against the human complement system. Glycobiology, Oxford University Press (OUP), 2003, 13, pp.29G-30G. ⟨10.1093/glycob/cwg126⟩. ⟨hal-00163296⟩
  • B. Tissot, Régis Daniel. Anticomplementary activity of fucoidan: determination of the targeted proteins and of their interactions. Immunology Letters, Elsevier, 2003, 87(1-3), pp.42. ⟨hal-00163301⟩
  • A. Varenne, P. Gareil, S. Colliec-Jouault, Régis Daniel. Capillary electrophoresis determination of the binding affinity of bioactive sulfated polysaccharides to proteins. Study of the binding properties of fucoidan to antithrombin. Analytical Biochemistry, Elsevier Masson, 2003, 315 (2), pp.152-159. ⟨10.1016/S0003-2697(02)00687-5⟩. ⟨hal-00163444⟩
  • S. Descroix, A. Varenne, N. Goasdoue, J. Abian, M. Carrascal, et al.. Non-aqueous capillary electrophoresis separation of positional isomers of sulfated carbohydrate. Journal of Chromatography A, Elsevier, 2003, 987, pp.467-476. ⟨hal-00163472⟩
  • O. Berteau, I. Mccort, N. Goasdoue, B. Tissot, Régis Daniel. Characterization of a new α-l-fucosidase isolated from the marine mollusk Pecten maximus that catalyzes the hydrolysis of α-l-fucose from algal fucoidan (Ascophyllum nodosum). Glycobiology, Oxford University Press (OUP), 2002, 12 (4), pp.273-282. ⟨10.1093/glycob/12.4.273⟩. ⟨hal-02047275⟩
  • Régis Daniel, Olivier Berteau, Lionel Chevolot, Anne Varenne, P. Gareil, et al.. Regioselective desulfation of sulfated l-fucopyranoside by a new sulfoesterase from the marine mollusk Pecten maximus. . European Journal of Biochemistry, Wiley, 2001, 268 (21), pp.5617-5626. ⟨10.1046/j.1432-1033.2001.02497.x⟩. ⟨hal-02047277⟩
  • S. Saillard, P. Gareil, J. Jozefonvicz, Régis Daniel. Development of a Capillary Electrophoresis Assay Based on Free Sulfate Determination for the Direct Monitoring of Sulfoesterase Activity. Analytical Biochemistry, Elsevier Masson, 1999, 275 (1), pp.11-21. ⟨hal-02047280⟩
  • Régis Daniel, Olivier Berteau, Jacqueline Jozefonvicz, Nicole Goasdoue. Degradation of algal (Ascophyllum nodosum) fucoidan by an enzymatic activity contained in digestive glands of the marine mollusc Pecten maximus. Carbohydrate Research, Elsevier, 1999, 322 (3-4), pp.291-297. ⟨10.1016/S0008-6215(99)00223-2⟩. ⟨hal-02047279⟩
  • Samy Ben Rejeb, Nathalie Fischer‐durand, Annie Martel, Régis Daniel, Claude Jolivalt, et al.. Purified polyclonal anti‐phenylurea antibodies for an improved immunoaffinity chromatography. Food and Agricultural Immunology, Taylor & Francis, 1998, 10 (3), pp.203-213. ⟨hal-02047281⟩
  • Régis Daniel, Eliane Caminade, Annie Martel, François Le Goffic, Daniel Daniel, et al.. Mass Spectrometric Determination of the Cleavage Sites in Escherichia coli Dihydroorotase Induced by a Cysteine-specific Reagent. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1997, 272 (43), pp.26934-26939. ⟨hal-02047284⟩
  • D. Canosa, Régis Daniel, D. Barceló, E. Gelpí, F. Le Goffic, et al.. Determination of pesticide epitopic density in protein immunoconjugates by electrospray mass spectrometry. International journal of mass spectrometry and ion processes, Elsevier, 1997, 160 (1-3), pp.395-407. ⟨10.1016/S0168-1176(96)04523-5⟩. ⟨hal-03133982⟩
  • N. Fischer-Durand, Régis Daniel, V. Pichon, L. Chen, M.-C. Hennion, et al.. Immunopreconcentration for the analysis of traces of pesticides in environment. Agro-food-Industry Hi Tech, Teknoscienze Srl, 1997, 8 (1), pp.21-24. ⟨hal-03140585⟩
  • Régis Daniel, B. Kokel, E. Caminade, A. Martel, F. Le Goffic. Assay ofEscherichia coliDihydroorotase with Enantiomeric Substrate: Practical Preparation of Carbamyll-Aspartate and High-Performance Liquid Chromatography Analysis of Catalysis Product. Analytical Biochemistry, Elsevier Masson, 1996, 239 (2), pp.130-135. ⟨10.1006/abio.1996.0307⟩. ⟨hal-03133980⟩
  • V. Pichon, L. Chen, M.-C. Hennion, Régis Daniel, A. Martel, et al.. Preparation and Evaluation of Immunosorbents for Selective Trace Enrichment of Phenylurea and Triazine Herbicides in Environmental Waters. Analytical Chemistry, American Chemical Society, 1995, 67 (14), pp.2451-2460. ⟨10.1021/ac00110a021⟩. ⟨hal-03133979⟩
  • Régis Daniel, J. Dorizon, D. Deprez. Determination of Lipoxygenase Activity by Continuous Flow Amperometric Detection. Analytical Biochemistry, Elsevier Masson, 1994, 219 (1), pp.154-155. ⟨10.1006/abio.1994.1245⟩. ⟨hal-03133977⟩
  • A. Hua, Régis Daniel, M. Jasseron, C. Thiriot. Early cytotoxic effects induced by bis-chloroethyl sulphide (sulphur mustard): [Ca2+]i rise and time-dependent inhibition of B77 fibroblast serum response. Journal of Applied Toxicology, Wiley, 1993, 13 (3), pp.161-168. ⟨10.1002/jat.2550130304⟩. ⟨hal-03133976⟩
  • C. Cucurou, J.P. Battioni, Régis Daniel, D. Mansuy. Peroxidase-like activity of lipoxygenases: different substrate specificity of potato 5-lipoxygenase and soybean 15-lipoxygenase and particular affinity of vitamin E derivatives for the 5-lipoxygenase. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Elsevier, 1991, 1081 (1), pp.99-105. ⟨10.1016/0005-2760(91)90256-h⟩. ⟨hal-03133973⟩
  • I. Michaud-Soret, Régis Daniel, C. Chopard, D. Mansuy, C. Cucurou, et al.. Soybean lipoxygenases-1, -2a, -2b and -2c do not contain PQQ.. Biochemical and Biophysical Research Communications, Elsevier, 1990, 172 (3), pp.1122-8. ⟨10.1016/0006-291X(90)91563-8⟩. ⟨hal-01063007⟩

Book sections1 document

  • Anastasiia Halushkina, William Buchmann, Nathalie Jarroux, Régis Daniel. Surface Plasmon Resonance Imaging-Mass Spectrometry Coupling on Antibody Array Biochip: Multiplex Monitoring of Biomolecular Interactions and On-Chip Identification of Captured Antigen. Whittaker, Kelly C., Huang, Ruo-Pan. Antibody Arrays, Methods and Protocols, Springer US, pp.55-67, 2020, Antibody Arrays, 978-1-0716-1063-3. ⟨10.1007/978-1-0716-1064-0_5⟩. ⟨hal-03098106⟩

Preprints, Working Papers, ...2 documents

  • Régis Daniel, Salomé Poyer, Ilham Seffouh, Chrystel Lopin-Bon, Jean-Claude Jacquinet, et al.. Discrimination of sulfated isomers of chondroitin sulfate disaccharides by HILIC chromatography and on-line coupled tandem mass spectrometry. 2021. ⟨hal-03232999⟩
  • Rana El Masri, Amal Seffouh, Caroline Roelants, Ilham Seffouh, Evelyne Gout, et al.. Extracellular endosulfatase Sulf-2 harbours a chondroitin/dermatan sulfate chain that modulates its enzyme activity. 2021. ⟨hal-03099200⟩

Patents2 documents

  • Régis Daniel, Sophie Gornard, Erven Hémon, Vincent Lognoné, Hélène Marfaing. BACTERIAL STRAIN AND BACTERIAL MIXTURE WITH A FUCANOLYTIC ACTIVITY. France, Patent n° : EP2376621. 2018. ⟨hal-03142416⟩
  • Régis Daniel, Florence Gonnet, William Buchmann, Sophie Bellon, Nathalie Jarroux, et al.. FUNCTIONALISED BIOCHIPS FOR SPR-MS COUPLING. France, Patent n° : EP2240772. 2016. ⟨hal-03142422⟩