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Marc Boudvillain

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Documents
Identifiants chercheurs

Présentation

- **CNRS Research director** and group Leader at CBM - **Deputy director** of CBM since January 2018. Education --------- - **PhD Thesis** in Biochemistry and Molecular Biophysics, University of Orléans (1996). - **Master of Science** (specialty: Biological Chemistry) from the Universities of Orléans, Paris Descartes, and Paris Sud (joint program, 1992). Professional experience ----------------------- - **Tenured Scientist** at CNRS, CBM since October 1999. Biochemistry of RNA and RNA-protein complexes; Regulation of bacterial transcription; RNA helicase mechanisms - **Postdoctoral Research Scientist** at Columbia University and Howard Hugues Medical Institute (New York, USA). Biochemistry of RNA; Structure, function and catalysis by group II intron ribozymes (1996-1999). - **Graduate Research Scientist** at CBM. Nucleic acid Biochemistry; Inorganic chemistry; Engineering of synthetic Pt(II)-based metalloribozymes (1993-1996).

Publications

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A scalable framework for the discovery of functional helicase substrates and helicase-driven regulatory switches

Mildred Delaleau , Eric Eveno , Isabelle Simon , Annie Schwartz , Marc Boudvillain
Proceedings of the National Academy of Sciences of the United States of America, 2022, 119 (38), ⟨10.1073/pnas.2209608119⟩
Article dans une revue hal-03800009v1
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Cryo-EM structure of transcription termination factor Rho from Mycobacterium tuberculosis reveals bicyclomycin resistance mechanism

Emmanuel Saridakis , Rishi Vishwakarma , Josephine Lai-Kee-Him , Kevin Martin , Isabelle Simon
Communications Biology, 2022, 5 (1), pp.120. ⟨10.1038/s42003-022-03069-6⟩
Article dans une revue hal-03563179v1
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Posttranscriptional Regulation by Copper with a New Upstream Open Reading Frame

Gauthier Roy , Antoine Rudy , Annie Schwartz , Stéphanie Slupek , Alex Rivera-Millot
Article dans une revue hal-03759142v2
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A large insertion domain in the Rho factor from a low G+C, Gram-negative bacterium is critical for RNA binding and transcription termination activity

Isabelle Simon , Mildred Delaleau , Annie Schwartz , Marc Boudvillain
Journal of Molecular Biology, 2021, 433 (15), pp.167060. ⟨10.1016/j.jmb.2021.167060⟩
Article dans une revue hal-03248336v1
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Regulatory interplay between small RNAs and transcription termination factor Rho

Lionello Bossi , Nara Figueroa-Bossi , Philippe Bouloc , Marc Boudvillain
Biochimica et Biophysica Acta - Gene Regulatory Mechanisms , 2020, pp.194546. ⟨10.1016/j.bbagrm.2020.194546⟩
Article dans une revue hal-02533337v1

A Fluorogenic Assay To Monitor Rho-Dependent Termination of Transcription

Cédric Nadiras , Mildred Delaleau , Annie Schwartz , Emmanuel Margeat , Marc Boudvillain
Biochemistry, 2019, 58 (7), pp.865-874. ⟨10.1021/acs.biochem.8b01216⟩
Article dans une revue hal-02067416v1

NusG prevents transcriptional invasion of H-NS-silenced genes

Lionello Bossi , Mathilde Ratel , Camille Laurent , Patricia Kerboriou , Andrew Camilli
PLoS Genetics, 2019, 15 (10), pp.e1008425. ⟨10.1371/journal.pgen.1008425⟩
Article dans une revue hal-02308418v1

A multivariate prediction model for Rho-dependent termination of transcription

Cédric Nadiras , Eric Eveno , Annie Schwartz , Nara Figueroa-Bossi , Marc Boudvillain
Nucleic Acids Research, 2018, 46 (16), pp.8245 - 8260. ⟨10.1093/nar/gky563⟩
Article dans une revue hal-01880575v1

Transcription Termination: Variations on Common Themes

Odil Porrua , Marc Boudvillain , Domenico Libri
Trends in Genetics, 2016, 32 (8), pp.508 - 522. ⟨10.1016/j.tig.2016.05.007⟩
Article dans une revue hal-01407669v1
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ATP-dependent motor activity of the transcription termination factor Rho from Mycobacterium tuberculosis

F. d'Heygere , A. Schwartz , F. Coste , B. Castaing , M. Boudvillain
Nucleic Acids Research, 2015, 43 (12), pp.6099-60111. ⟨10.1093/nar/gkv505⟩
Article dans une revue hal-01171219v1
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Direct observation of the translocation mechanism of transcription termination factor Rho

V. Gocheva , Antoine Le Gall , M. Boudvillain , E. Margeat , Marcelo Nollmann
Nucleic Acids Research, 2015, 43 (4), pp.2367-2377. ⟨10.1093/nar/gkv085⟩
Article dans une revue hal-01171254v1
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The RNA-mediated, asymmetric ring regulatory mechanism of the transcription termination Rho helicase decrypted by time-resolved nucleotide analog interference probing (trNAIP).

Emilie Soares , Annie Schwartz , Marcelo Nollmann , Emmanuel Margeat , Marc Boudvillain
Nucleic Acids Research, 2014, 42 (14), pp.9270-9284. ⟨10.1093/nar/gku595⟩
Article dans une revue hal-01180888v1

RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination

N. Figueroa-Bossi , A. Schwartz , B. Guillemardet , F. d'Heygere , L. Bossi
Genes and Development, 2014, 28 (11), pp.1239-1251. ⟨10.1101/gad.240192.114⟩
Article dans une revue hal-01178971v1

Phyletic distribution and conservation of the bacterial transcription termination factor Rho.

François d'Heygère , Makhlouf Rabhi , Marc Boudvillain
Microbiology, 2013, epub ahead of print. ⟨10.1099/mic.0.067462-0⟩
Article dans une revue hal-00840167v1

Terminator still moving forward: expanding roles for Rho factor.

Marc Boudvillain , Nara Figueroa-Bossi , Lionello Bossi
Current Opinion in Microbiology, 2013, 16 (2), pp.118-24. ⟨10.1016/j.mib.2012.12.003⟩
Article dans une revue hal-00840158v1

Transcription termination controls prophage maintenance in Escherichia coli genomes.

Rachid Menouni , Stéphanie Champ , Leon Espinosa , Marc Boudvillain , Mireille Ansaldi
Proceedings of the National Academy of Sciences of the United States of America, 2013, 110 (35), pp.14414-9. ⟨10.1073/pnas.1303400110⟩
Article dans une revue hal-00874882v1

Tight intramolecular regulation of the human Upf1 helicase by its N- and C-terminal domains

Francesca Fiorini , Marc Boudvillain , Hervé Le Hir
Nucleic Acids Research, 2013, 41 (4), pp.2404-2415. ⟨10.1093/nar/gks1320⟩
Article dans une revue hal-02358926v1

A role for Rho-dependent polarity in gene regulation by a noncoding small RNA.

Lionello Bossi , Annie Schwartz , Benoit Guillemardet , Marc Boudvillain , Nara Figueroa-Bossi
Genes and Development, 2012, 26 (16), pp.1864-1873. ⟨10.1101/gad.195412.112⟩
Article dans une revue hal-00727260v1

Analysis of helicase-RNA interactions using nucleotide analog interference mapping.

Annie Schwartz , Makhlouf Rabhi , Emmanuel Margeat , Marc Boudvillain
Methods in Enzymology, 2012, 511, pp.149-69. ⟨10.1016/B978-0-12-396546-2.00007-3⟩
Article dans une revue hal-00726256v1

Mutagenesis-Based Evidence for an Asymmetric Configuration of the Ring-Shaped Transcription Termination Factor Rho

Makhlouf Rabhi , Veronika Gocheva , Frederique Jacquinot , Andrea Lee , Emmanuel Margeat
Journal of Molecular Biology, 2011, 405 (2), pp.497-518. ⟨10.1016/j.jmb.2010.11.006⟩
Article dans une revue hal-00608241v1

The Sm-like RNA chaperone Hfq mediates transcription antitermination at Rho-dependent terminators.

Makhlouf Rabhi , Olivier Espéli , Annie Schwartz , Bastien Cayrol , a Rachid Rahmouni
EMBO Journal, 2011, 30 (14), pp.2805-16. ⟨10.1038/emboj.2011.192⟩
Article dans une revue hal-00720713v1

RNA remodeling by hexameric RNA helicases

Makhlouf Rabhi , Roman Tuma , Marc Boudvillain
RNA Biology, 2010, 7 (6 - Sp. Iss. SI), pp.655-666. ⟨10.4161/rna.7.6.13570⟩
Article dans une revue hal-00602496v1

Keeping up to speed with the transcription termination factor Rho motor.

Marc Boudvillain , Marcelo Nollmann , Emmanuel Margeat
Transcription, 2010, 1 (2), pp.70-75. ⟨10.4161/trns.1.2.12232⟩
Article dans une revue hal-00591655v1

A stepwise 2 '-hydroxyl activation mechanism for the bacterial transcription termination factor Rho helicase

Annie Schwartz , Makhlouf Rabhi , Frederique Jacquinot , Emmanuel Margeat , A. Rachid Rahmouni
Nature Structural and Molecular Biology, 2009, 16 (12), pp.1309-1316. ⟨10.1038/nsmb.1711⟩
Article dans une revue hal-00525325v1
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Transcription termination factor Rho can displace streptavidin from biotinylated RNA

Annie Schwartz , Emmanuel Margeat , A. Rachid Rahmouni , Marc Boudvillain
Journal of Biological Chemistry, 2007, 282 (43), pp.31469-31476. ⟨10.1074/jbc.M706935200⟩
Article dans une revue hal-00176284v1

Noncanonical Interactions in the Management of RNA Structural Blocks by the Transcription Termination Rho Helicase

Annie Schwartz , Céline Walmacq , A. Rachid Rahmouni , Marc Boudvillain
Biochemistry, 2007, 46 (33), pp.9366 - 9379. ⟨10.1021/bi700493m⟩
Article dans une revue hal-00166551v1

Testing the steric exclusion model for hexameric helicases: substrate features that alter RNA-DNA unwinding by the transcription termination factor Rho

C. Walmacq , A.R. Rahmouni , M. Boudvillain
Biochemistry, 2006, 45, pp.5885-5895. ⟨10.1021/bi0600648⟩
Article dans une revue hal-00088941v1

Influence of substrate composition on the helicase activity of transcription termination factor Rho: reduced processivity of Rho hexamers during unwinding of RNA-DNA hybrid regions

C. Walmacq , A.R. Rahmouni , M. Boudvillain
Journal of Molecular Biology, 2004, 342 (2), pp.403-420. ⟨10.1016/j.jmb.2004.07.026⟩
Article dans une revue hal-00113090v1

The functional anatomy of an intrinsic transcription terminator

A. Schwartz , A. Rachid Rahmouni , Marc Boudvillain
EMBO Journal, 2003, 22 (13), pp.3385 - 3394. ⟨10.1093/emboj/cdg310⟩
Article dans une revue hal-01880611v1

Limited Topological Alteration of the T7 RNA Polymerase Active Center at Intrinsic Termination Sites †

Marc Boudvillain , Annie Schwartz , A. Rachid Rahmouni
Biochemistry, 2002, 41 (9), pp.3137-3146. ⟨10.1021/bi011358q⟩
Article dans une revue hal-02075291v1

Monofunctionallytrans-Diammine Platinum(II)-Modified Peptide Nucleic Acid Oligomers: A New Generation of Potential Antisense Drugs

Kathrin Schmidt , Marc Boudvillain , Annie Schwartz , Gijs van Der Marel , Jacques van Boom
Chemistry - A European Journal, 2002, 8 (24), pp.5566 - 5570. ⟨10.1002/1521-3765(20021216)8:24<5566::AID-CHEM5566>3.0.CO;2-#⟩
Article dans une revue hal-01880614v1

Oligonucleotides Modified With Transplatin Derivatives: Fast and Efficient Metalloribozymes

Boudvillain Marc , Rozenn Dalbies-Tran , Marc Leng , Marc Boudvillain
Metal-Based Drugs, 2001, 8 (1), pp.39 - 45. ⟨10.1155/MBD.2001.39⟩
Article dans une revue hal-01880601v1

A tertiary interaction that links active-site domains to the 5′ splice site of a group II intron

Marc Boudvillain , Alexandre de Lencastre , Anna Marie Pyle
Nature, 2000, 406 (6793), pp.315 - 318. ⟨10.1038/35018589⟩
Article dans une revue hal-01880615v1

Parallel-stranded DNA with Hoogsteen base pairing stabilized by a trans-[Pt(NH3)2]2+ cross-link: characterization and conversion into a homodimer and a triplex

Jens Müller , Markus Drumm , Marc Boudvillain , Marc Leng , Einar Sletten
Journal of Biological Inorganic Chemistry, 2000, 5 (5), pp.603-611. ⟨10.1007/s007750000143⟩
Article dans une revue hal-02075297v1

NMR solution structure of a DNA dodecamer containing a transplatin interstrand GN7-CN3 cross-link

Françoise Paquet , Marc Boudvillain , Gérard Lancelot , Marc Leng
Nucleic Acids Research, 1999, 27 (21), pp.4261-4268. ⟨10.1093/nar/27.21.4261⟩
Article dans une revue hal-02075311v1

Using DNAzymes to cut, process, and map RNA molecules for structural studies or modification.

Anna M. Pyle , V Chu , E Jankowsky , Marc Boudvillain
Methods in Enzymology, 1999, 317, pp.140-6
Article dans une revue hal-02096724v1

Defining functional groups, core structural features and inter-domain tertiary contacts essential for group II intron self-splicing: a NAIM analysis

Marc Boudvillain , Anna M. Pyle
EMBO Journal, 1998, 17 (23), pp.7091-7104. ⟨10.1093/emboj/17.23.7091⟩
Article dans une revue hal-02096717v1

Distortions of the DNA Double Helix Induced by 1,3-trans-Diamminedichloroplatinum(II)-intrastrand Cross-link: An Internal Coordinate Molecular Modeling Study

Marc Boudvillain , C. Prevost , P. Beudaert , M. Leng , R. Lavery
Journal of Biomolecular Structure and Dynamics, 1997, 14 (6), pp.703 - 714. ⟨10.1080/07391102.1997.10508173⟩
Article dans une revue hal-01880624v1

Transplatin-Modified Oligo(2‘- O -methyl ribonucleotide)s: A New Tool for Selective Modulation of Gene Expression †

Marc Boudvillain , Martine Guerin , Rozenn Dalbies , Tula Saison-Behmoaras , Marc Leng
Biochemistry, 1997, 36 (10), pp.2925 - 2931. ⟨10.1021/bi962695f⟩
Article dans une revue hal-01880625v1

Interstrand Crosslinking Reaction in Transplatin-Modified Oligo-2‵-O-Methyl Ribonucleotide-RNA Hybrids

Caroline Colombier , Marc Boudvillain , Marc Leng
Antisense and Nucleic Acid Drug Development, 1997, 7 (4), pp.397-402. ⟨10.1089/oli.1.1997.7.397⟩
Article dans une revue hal-02322135v1

Chemical Versatility of Transplatin Monofunctional Adducts within Multiple Site-Specifically Platinated DNA †

Eloy Bernal-Méndez , Marc Boudvillain , Francisco González-Vílchez , Marc Leng
Biochemistry, 1997, 36 (24), pp.7281 - 7287. ⟨10.1021/bi9703148⟩
Article dans une revue hal-01880622v1

Distortions of the DNA double helix induced by 1,3-trans-diamminedichloroplatinum(II)-intrastrand cross-link: an internal coordinate molecular modeling study.

C. Prevost , M. Boudvillain , P. Beudaert , M. Leng , R. Lavery
Journal of Biomolecular Structure and Dynamics, 1997, 14, pp.703-714
Article dans une revue hal-00313421v1

Evidences for a catalytic activity of the DNA double helix in the reaction between DNA, platinum(II), and intercalators.

Marc Boudvillain , Rozenn Dalbies , M. Leng
Metal Ions in Biological Systems, 1996, 33, pp.87-104
Article dans une revue hal-02322086v1

Intrastrand cross-links are not formed in the reaction between transplatin and native DNA: relation with the clinical inefficency of transplatin

Marc Boudvillain , Rozenn Dalbies , Christophe Aussourd , Marc Leng
Nucleic Acids Research, 1995, 23 (13), pp.2381-2388. ⟨10.1093/nar/23.13.2381⟩
Article dans une revue hal-02075318v1

Linkage isomerization reaction of intrastrand cross-links in trans -diamminedichloroplatinum(II)-modified single-stranded oligonucleotides

Rozenn Dalbies , Marc Boudvillain , Marc Leng
Nucleic Acids Research, 1995, 23 (6), pp.949-953. ⟨10.1093/nar/23.6.949⟩
Article dans une revue hal-02075321v1

Helicase-SELEX: A versatile new approach to seek constitutive or conditional helicase substrates at global scale

Mildred Delaleau , Eric Eveno , Annie Schwartz , Isabelle Simon , Marc Boudvillain
12th SifrARN: Structure, integration, function & reactivity of RNA, Oct 2022, Bordeaux, France
Communication dans un congrès hal-04148466v1

Régulation des gènes par un moteur moléculaire unique aux bactéries

Marc Boudvillain
34e Colloque de Biotechnocentre, Oct 2022, Nouan-le-Fuzelier, France
Communication dans un congrès hal-04148476v1

Structural basis for resistance of Mycobacterium tuberculosis Rho to bicyclomycin

Emmanuel Saridakis , Rishi Vishwakarma , Joséphine Lai Kee Him , Kevin Martin , Isabelle Simon
Helicases & Nucleic Acid-Based Machines: Structure, Mechanism & Regulation, and Roles in HumanDisease, Jul 2021, Paris, France
Communication dans un congrès hal-03419029v1

Variation on a theme - the unusual features of the essential transcription termination Rho factor from a low G+C, Gram-negative bacterium

Isabelle Simon , Mildred Delaleau , Annie Schwartz , Marc Boudvillain
45th FEBS congress, Jul 2021, Lubjana, Slovenia
Poster de conférence hal-03419044v1

Exploring Rho helicase activity for the purpose of synthetic regulation

I. Simon , A. Schwartz , M. Delaleau , L. Bossi , N. Figueroa-Bossi
32e Colloque Biotechnocentre, Oct 2019, Seillac, France
Poster de conférence hal-02902096v1

Predicting Rho-dependent termination of transcription at genome scale

E. Eveno , C. Nadiras , N. Figueroa-Bossi , A. Schwartz , M. Boudvillain
24th Annual Meeting of the RNA Society, Jun 2019, Cracovie, Poland
Poster de conférence hal-02902104v1

Genome-scale prediction of Rho-dependent termination of transcription

E. Eveno , C. Nadiras , A. Schwartz , N. Figueroa-Bossi , M. Boudvillain
11e Rencontre SifrARN (SFBBM), Nov 2018, Nancy, France
Poster de conférence hal-02902107v1

Single-molecule analysis of E. coli Rho translocase, Helicase, transcription termination activities, and its interactions with RNA polymerase

R. Vishwakarma , A. Nord , F. Pedaci , M. Boudvillain , E. Margeat
Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France
Poster de conférence hal-02902115v1

Relationships between Rho and RNA polymerase in living bacteria

C. Clerte , O. Manier , J-B. Fiche , M. Boudvillain , L. Bossi
Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France
Poster de conférence hal-02902111v1

Seeking Rho-dependent transcription termination sites within bacterial genomes

C. Nadiras , E. Eveno , M. Boudvillain
EMBO & Harden conference - Helicases and Nucleic Acid Translocases, Jul 2017, Kloster Banz-Bamberg, Germany
Poster de conférence hal-02902120v1

Identification des sites de terminaison de transcription Rho-dépendantes dans les génomes bactériens

C. Nadiras , E. Eveno , M. Boudvillain
30e Colloque Biotechnocentre, Oct 2017, Seillac, France
Poster de conférence hal-02902116v1

Towards a model for predicting Rho-dependent transcription termination sites

C. Nadiras , E. Eveno , M. Boudvillain
Le Studium conference “Structural biology and biophysics of RNA-protein complexes”, Nov 2017, Orléans, France
Poster de conférence hal-02902112v1

Analyse des terminateurs Rho-dépendants bactériens

C. Nadiras , Eric Eveno , A. Schwartz , M. Boudvillain
29e colloque Biotechnocentre, Oct 2016, Seillac, France
Poster de conférence hal-02453368v1
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A simple fluorescence microplate assay to monitor RNA-DNA hybrid unwinding by the bacterial transcription termination factor Rho

Isabelle Simon , Marc Boudvillain
Marc Boudvillain. RNA remodeling proteins : Methods and Protocols, 2209, Humana press, pp.143-161, 2021, Methods in Molecular Biology, 978-1-0716-0937-8. ⟨10.1007/978-1-0716-0935-4_10⟩
Chapitre d'ouvrage hal-03036494v1
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Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-Dependent Termination of Transcription In Vitro.

Cédric Nadiras , Annie Schwartz , Mildred Delaleau , Marc Boudvillain
Methods in Mecular Biology, 1737, humana press, Totowa, NJ-USA, pp.99-118, 2018, Bacterial regulatory RNA (Arluison & Valverde, eds), ⟨10.1007/978-1-4939-7634-8_7⟩
Chapitre d'ouvrage hal-01880631v1

Monitoring RNA Unwinding by the Transcription Termination Factor Rho from Mycobacterium tuberculosis

F. d'Heygère , A. Schwartz , F. Coste , B. Castaing , M. Boudvillain
Methods in Mecular Biology, 1259, Springer, pp.293-311, 2015, RNA remodelling proteins (Boudvillain, ed), 978-1-4939-2213-0. ⟨10.1007/978-1-4939-2214-7_18⟩
Chapitre d'ouvrage hal-01174055v1

Nucleotide Analog Interference Mapping and Suppression (NAIM/NAIS): a Combinatorial Approach to Study RNA Structure, Folding, and Interaction with Proteins

Olga Fedorova , Marc Boudvillain , Christina Waldsich
Handbook of RNA Biochemistry, 2nd edition (Westhof, Bindereif, Schon & Hartmann, eds), Wiley-VCH Verlag GmbH & Co. KGaA, pp.329-368, 2014, ⟨10.1002/9783527647064.ch17⟩
Chapitre d'ouvrage hal-02097735v1

Transcription termination factor Rho: a ring-shaped RNA helicase from bacteria

Makhlouf Rabhi , Rahmouni Rachid A. , Marc Boudvillain
Eckhard Jankowsky. RNA helicases, Royal Society of Chemistry Publishing, pp.243-271, 2010, ⟨10.1039/9781849732215-00243⟩
Chapitre d'ouvrage hal-00610004v1

Simple enzymatic assays for the in vitro motor activity of transcription termination factor Rho from Escherichia coli

Marc Boudvillain , Céline Walmacq , Annie Schwartz , Frédérique Jacquinot
Mohamed M. M. Abdelhaleem. Methods in Mecular Biology, Humana Press, pp.137-154, 2009, Helicases (Abdelhaleem, ed), ⟨10.1007/978-1-60327-355-8_10⟩
Chapitre d'ouvrage hal-00623571v1

Nucleotide analog interference mapping and suppression (NAIM/NAIS): specific applications in studies of RNA tertiary structure, dynamic helicase mechanism, and RNA-protrein interactions

O. Fedorova , M. Boudvillain , J. Kawaola , A.M. Pyle
E. Westhof, A. Bindereif, A. Schon, R.I. Hartmann. Handbook of RNA biochemistry, Wiley-VCH, pp.259-293, 2005
Chapitre d'ouvrage hal-00088591v1

Trans-Diamminedichloroplatinum (II) Is Not an Antitumor Drug: Why?

Marc Boudvillain , Rozenn Dalbies-Tran , Leng M.
Platinum and Other Metal Coordination Compounds in Cancer Chemotherapy, 2, Plenum Press, New'Tork, USA, pp.17-25, 1996
Chapitre d'ouvrage hal-02097755v1