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Amphipathic environments for determining the structure of membrane proteins by single-particle electron cryo-microscopy

Christel Le Bon , Baptiste Michon , Jean-Luc Popot , M. Zoonens
Quarterly Reviews of Biophysics, 2021, 54, pp.e6. ⟨10.1017/S0033583521000044⟩
Article dans une revue hal-03322455v1
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Cycloalkane-modified amphiphilic polymers provide direct extraction of membrane proteins for CryoEM analysis

Anna J Higgins , Alex J Flynn , Anaïs Marconnet , Laura J Musgrove , Vincent L G Postis , et al.
Communications Biology, 2021, ⟨10.1038/s42003-021-02834-3⟩
Article dans une revue hal-03448731v1

Dynamics of membrane protein/amphipol association studied by Förster resonance energy transfer. Implications for in vitro studies of amphipol-stabilized membrane proteins

Manuela Zoonens , Fabrice Giusti , Francesca Zito , Jean-Luc Popot
Biochemistry, 2007, 46 (36), pp.10392-10404
Article dans une revue hal-00169697v1

Free-standing films of fluorinated surfactants as 2D matrices for organizing detergent-solubilized membrane protein

Vera Petkova , Jean-Jacques Benattar , Manuela Zoonens , Francesca Zito , Jean-Luc Popot , et al.
Langmuir, 2007, 23, pp.4303-4309
Article dans une revue hal-00169090v1

Stabilization of a Membrane-Associated Amyloid-β Oligomer for Its Validation in Alzheimer's Disease

Montserrat Serra-Batiste , James Tolchard , Fabrice Giusti , Manuela Zoonens , Natàlia Carulla
Frontiers in Molecular Biosciences, 2018, 5, ⟨10.3389/fmolb.2018.00038⟩
Article dans une revue hal-02123752v1
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Role of surfactants in electron cryo-microscopy film preparation

Baptiste Michon , Uriel López-Sánchez , Jéril Degrouard , Hugues Nury , Amélie Leforestier , et al.
Biophysical Journal, 2023, 122 (10), pp.1846-1857. ⟨10.1016/j.bpj.2023.04.016⟩
Article dans une revue hal-04271843v1

Assaying the proton transport and regulation of UCP1 using solid supported membranes.

Iulia Blesneac , Stéphanie Ravaud , Paul Machillot , Manuela Zoonens , Sandrine Masscheylen , et al.
Biophysics of structure and mechanism, 2012, 41 (8), pp.675-9
Article dans une revue hal-01179678v1

Co-delivery of amphipol-conjugated adjuvant with antigen, and adjuvant combinations, enhance immune protection elicited by a membrane protein-based vaccine against a mucosal challenge with Chlamydia

Delia Tifrea , Sukumar Pal , Christel Le Bon , Fabrice Giusti , Jean-Luc Popot , et al.
Vaccine, 2018, 36 (45), pp.6640-6649. ⟨10.1016/j.vaccine.2018.09.055⟩
Article dans une revue hal-02324125v1
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Influence of hydrophobic groups attached to amphipathic polymers on the solubilization of membrane proteins along with their lipids

Anaïs Marconnet , Baptiste Michon , Bastien Prost , Audrey Solgadi , Christel Le Bon , et al.
Analytical Chemistry, 2022, ⟨10.1021/acs.analchem.2c01746⟩
Article dans une revue hal-03813679v1

Amphipol-Trapped ExbB–ExbD Membrane Protein Complex from Escherichia coli: A Biochemical and Structural Case Study

Aleksandr Sverzhinsky , Shuo Qian , Lin Yang , Marc Allaire , Isabel Moraes , et al.
Journal of Membrane Biology, 2014, 247 (9-10), pp.1005-1018. ⟨10.1007/s00232-014-9678-4⟩
Article dans une revue hal-02542086v1

NMR study of a membrane protein in detergent-free aqueous solution

M. Zoonens , L. Catoire , F. Giusti , J.-L. Popot
Proceedings of the National Academy of Sciences of the United States of America, 2005, 102 (25), pp.8893-8898. ⟨10.1073/pnas.0503750102⟩
Article dans une revue hal-03011683v1

Synthesis of a Polyhistidine-bearing Amphipol and its Use for Immobilizing Membrane Proteins

Fabrice Giusti , Pascal Kessler , Randi Westh Hansen , Eduardo Della Pia , Christel Le Bon , et al.
Biomacromolecules, 2015, 16 (12), pp.3751-3761. ⟨10.1021/acs.biomac.5b01010⟩
Article dans une revue hal-02123745v1

Inter- and intramolecular contacts in a membrane protein/surfactant complex observed by heteronuclear dipole-to-dipole cross-relaxation.

Laurent J Catoire , Manuela Zoonens , Carine van Heijenoort , Fabrice Giusti , Jean-Luc Popot , et al.
Journal of Magnetic Resonance, 2009, 197 (1), pp.91-5. ⟨10.1016/j.jmr.2008.11.017⟩
Article dans une revue istex hal-00373616v1
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Improved protection against Chlamydia muridarum using the native major outer membrane protein trapped in Resiquimod-carrying amphipols and effects in protection with addition of a Th1 (CpG-1826) and a Th2 (Montanide ISA 720) adjuvant.

Delia F Tifrea , Sukumar Pal , Christel Le Bon , Melanie J Cocco , Manuela Zoonens , et al.
Vaccine, 2020, 38 (28), pp.4412-4422
Article dans une revue hal-03019387v1

NMR study of a membrane protein in detergent-free aqueous solution

M. Zoonens , L. Catoire , F. Giusti , J.-L. Popot
Proceedings of the National Academy of Sciences of the United States of America, 2005, 102 (25), pp.8893-8898. ⟨10.1073/pnas.0503750102⟩
Article dans une revue hal-02324103v1

Membrane Protein Production in Escherichia coli: Overview and Protocols

Georges Hattab , Annabelle Suisse , Oana Ilioaia , Marina Casiraghi , Manuela Dezi , et al.
Membrane Proteins Production for Structural Analysis, Springer New York, pp.87-106, 2014, ⟨10.1007/978-1-4939-0662-8_4⟩
Chapitre d'ouvrage hal-02567629v1

A Step Closer to Membrane Protein Multiplexed Nanoarrays Using Biotin-Doped Polypyrrole

Eduardo Antonio Della Pia , Jeppe Holm , Noemie Lloret , Christel Le Bon , Jean-Luc Popot , et al.
ACS Nano, 2014, 8 (2), pp.1844-1853. ⟨10.1021/nn406252h⟩
Article dans une revue hal-02542092v1
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Solubilization and stabilization of membrane proteins by cycloalkane-modified amphiphilic polymers.

Anaïs Marconnet , Baptiste Michon , Christel Le Bon , Fabrice Giusti , Christophe Tribet , et al.
Biomacromolecules, 2020, 21, pp.3459−3467. ⟨10.1021/acs.biomac.0c00929⟩
Article dans une revue hal-03018338v1

Dangerous Liaisons between Detergents and Membrane Proteins. The Case of Mitochondrial Uncoupling Protein 2

Manuela Zoonens , Jeffrey Comer , Sandrine Masscheleyn , Eva Pebay-Peyroula , Christophe Chipot , et al.
Journal of the American Chemical Society, 2013, 135 (40), pp.15174-15182. ⟨10.1021/ja407424v⟩
Article dans une revue hal-02542096v1

Reconstitution and alignment by a magnetic field of a beta-barrel membrane protein in bicelles

Mohamed N. Triba , Manuela Zoonens , Jean-Luc Popot , Philippe F. Devaux , Dror E. Warschawski
European Biophysics Journal, 2006, 35, pp.268-275
Article dans une revue hal-00093076v1

Dynamics of Membrane Protein/Amphipol Association Studied by Förster Resonance Energy Transfer: Implications for in Vitro Studies of Amphipol-Stabilized Membrane Proteins †

Manuela Zoonens , Fabrice Giusti , Francesca Zito , Jean-Luc Popot
Biochemistry, 2007, 46 (36), pp.10392-10404. ⟨10.1021/bi7007596⟩
Article dans une revue istex hal-02123750v1

Slow Diffusion of Macromolecular Assemblies Measured by a New Pulsed Field Gradient NMR Method [ J. Am. Chem. Soc . 2003 , 125 , 2541−2545].

Fabien Ferrage , Manuela Zoonens , Dror E. Warschawski , Jean-Luc Popot , Geoffrey Bodenhausen
Journal of the American Chemical Society, 2004, 126 (17), pp.5654-5654. ⟨10.1021/ja033464g⟩
Article dans une revue hal-02567609v1

How Amphipols Embed Membrane Proteins: Global Solvent Accessibility and Interaction with a Flexible Protein Terminus

Manuel Etzkorn , Manuela Zoonens , Laurent Catoire , Jean-Luc Popot , Sebastian Hiller
Journal of Membrane Biology, 2014, 247 (9-10), pp.965-970. ⟨10.1007/s00232-014-9657-9⟩
Article dans une revue hal-03011645v1

Amphipols for Each Season

Manuela Zoonens , Jean-Luc Popot
Journal of Membrane Biology, 2014, 247 (9-10), pp.759-796. ⟨10.1007/s00232-014-9666-8⟩
Article dans une revue hal-02542083v1
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Full‐length G glycoprotein directly extracted from rabies virus with detergent and then stabilized by amphipols in liquid and freeze‐dried forms

Didier Clénet , Léna Clavier , Benoît Strobbe , Christel Le Bon , M. Zoonens , et al.
Biotechnology and Bioengineering, 2021, 118, pp.4317 - 4330. ⟨10.1002/bit.27900⟩
Article dans une revue hal-03426386v1

Amphipols: polymeric surfactants for membrane biology research

J.-L. Popot , E. Berry , D. Charvolin , C. Creuzenet , Christine Ebel , et al.
Cellular and Molecular Life Sciences, 2003, 60 (8), pp.1559-1574. ⟨10.1007/s00018-003-3169-6⟩
Article dans une revue istex hal-02327355v1

Folding and stabilizing membrane proteins in amphipol A8-35

Christel Le Bon , Anaïs Marconnet , Sandrine Masscheleyn , Jean-Luc Popot , Manuela Zoonens
Methods, 2018, 147, pp.95-105. ⟨10.1016/j.ymeth.2018.04.012⟩
Article dans une revue hal-02363736v1
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Amphipols From A to Z

Jean-Luc Popot , Thorsten Althoff , Dominique Bagnard , Jean-Louis Banères , Paola Bazzacco , et al.
Annual Review of Biophysics and Biomolecular Structure, 2011, 40 (1), pp.379-408. ⟨10.1146/annurev-biophys-042910-155219⟩
Article dans une revue hal-02327349v1

Solution NMR mapping of water-accessible residues in the transmembrane beta-barrel of OmpX

L. J. Catoire , M. Zoonens , C. van Heijenoort , F. Giusti , E. Guittet , et al.
European Biophysics Journal, 2010, 39 (4), pp.623-630. ⟨10.1007/s00249-009-0513-2⟩
Article dans une revue istex hal-00476867v1

Functionalized Amphipols: A Versatile Toolbox Suitable for Applications of Membrane Proteins in Synthetic Biology

Eduardo Antonio Della Pia , Randi Westh Hansen , Manuela Zoonens , Karen Martinez
Journal of Membrane Biology, 2014, 247 (9-10), pp.815-826. ⟨10.1007/s00232-014-9663-y⟩
Article dans une revue hal-02542088v1