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Isabelle Fudal

Dr Isabelle Fudal, INRAE Senior researcher, HDR
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**Address:** Unité Bioger (UR 1290) Team Effecteurs et pathogenèse chez *Leptosphaeria maculans* Batiment F. Campus AgroParisTech. 22 place de l'agronomie. 91120 Palaiseau. **Contact :** Tél. : +33 (0)6 80 78 46 33 Mail : [Isabelle.Fudal@inrae.fr](mailto:Isabelle) ### <a name="recherche"></a>Research <a name="enseignement"></a><a name="CV"></a>I am a research scientist hired at INRA in the BIOGER unit in 2006, with a background in plant pathology, molecular biology and functional genomics. My main focus was, and still is, on deciphering fungal-plant interactions. Most of my research experience was devoted to understand fungal pathogenicity and signals produced by the fungus, which induce recognition of “non-self” and disease resistance (avirulence genes or effectors). Since I was hired as a permanent scientist at INRA, I am developing functional genomics approaches on plant-fungi interactions with a particular interest to fungal effectors on the model of interaction *Leptosphaeria maculans / Brassica napus*. The objective of my research project is to study the possible roles of effectors on pathogenicity towards *B. napus* (identification, expression, regulation, evolution and functional analysis). To achieve this goal, I develop a pluri-disciplinary project combining bioinformatics, transcriptomics, functional genomics, pathogenicity studies and evolutionary genomics. Recently, I focused part of my researches on the determinism of concerted regulation of effector genes during infection and pointed out on the importance of chromatin status in this regulation. I am co-leading the INRA SPE network REacTION (network on the epigenetic mechanisms that shape plant - Bioaggressors and Symbiotic Organisms interactions) ### Curriculum vitae Since 2020 INRAE Senior Researcher, UMR1290 BIOGER, Grignon 2014 HDR, Université Paris-Sud, Orsay 2006-2019 INRA Researcher Scientist, UMR1290 Bioger, INRA, Grignon 2004-2006 Postdoc, Unité PMDV, INRA, Versailles 2000-2004 PhD, Université Paris-Sud, Orsay 1999-2000 Master Biologie, Diversité et Adaptation des Plantes Cultivées : option Phytopathologie (Paris) 1997- 2000 Engineer degree, Institut National Agronomique Paris-Grignon (INA P-G, Paris)

Publications

Regulation of effector gene expression as concerted waves in Leptosphaeria maculans : a two‐player game

Colin Clairet , Elise Gay , Antoine Porquier , Françoise Blaise , Claire‐line Marais
New Phytologist, 2024, ⟨10.1111/nph.19581⟩
Article dans une revue hal-04480387v1

Facilitation of symplastic effector protein mobility by paired effectors is conserved in different classes of fungal pathogens

Nacera Talbi , Mila Blekemolen , Slavica Janevska , Daniel Zendler , Herman van Tilbeurgh
Molecular Plant-Microbe Interactions, 2023, 37 (3), 304-314 p. ⟨10.1094/MPMI-07-23-0103-FI⟩
Article dans une revue hal-04232770v1

The neighbouring genes AvrLm10A and AvrLm10B are part of a large multigene family of cooperating effector genes conserved in Dothideomycetes and Sordariomycetes

Nacera Talbi , Like Fokkens , Corinne Audran , Yohann Petit-Houdenot , Cécile Pouzet
Molecular Plant Pathology, inPress, ⟨10.1111/MPP.13338⟩
Article dans une revue hal-04090329v1

Nucleosome patterns in four plant pathogenic fungi with contrasted genome structures

Colin Clairet , Nicolas Lapalu , Adeline Simon , Jessica Soyer , Muriel Viaud
Peer Community Journal, 2023, 3, pp.e13. ⟨10.24072/pcjournal.227⟩
Article dans une revue hal-04088414v1
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Improving sustainable crop protection using population genetics concepts

Méline Saubin , Clémentine Louet , Lydia Bousset , Frédéric Fabre , Pascal Frey
Molecular Ecology, 2023, 32 (10), pp.2461-2471. ⟨10.1111/mec.16634⟩
Article dans une revue hal-03394837v2
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A new family of structurally conserved fungal effectors displays epistatic interactions with plant resistance proteins

Noureddine Lazar , Carl Mesarich , Yohann Petit-Houdenot , Nacera Talbi , Ines Li de La Sierra-Gallay
PLoS Pathogens, 2022, 18 (7), pp.e1010664. ⟨10.1371/journal.ppat.1010664⟩
Article dans une revue hal-03324327v1
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A gene‐for‐gene interaction involving a ‘late’ effector contributes to quantitative resistance to the stem canker disease in Brassica napus

Audren Jiquel , Julie Gervais , Aude Geistodt‐kiener , Régine Delourme , Elise Gay
New Phytologist, 2021, 231 (4), pp.1510-1524. ⟨10.1111/nph.17292⟩
Article dans une revue hal-03155959v1
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Large-scale transcriptomics to dissect 2 years of the life of a fungal phytopathogen interacting with its host plant

Elise J Gay , Jessica L Soyer , Nicolas Lapalu , Juliette Linglin , Isabelle Fudal
BMC Biology, 2021, 19 (1), pp.e041118. ⟨10.1186/s12915-021-00989-3⟩
Article dans une revue hal-03190704v1
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Genome-wide mapping of histone modifications during axenic growth in two species of Leptosphaeria maculans showing contrasting genomic organization

Jessica Soyer , Colin Clairet , Elise Gay , Nicolas Lapalu , Thierry Rouxel
Chromosome Research, 2021, 29 (2), pp.219-236. ⟨10.1007/s10577-021-09658-1⟩
Article dans une revue hal-03266749v1
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A new avirulence gene of Leptosphaeria maculans , AvrLm14 , identifies a resistance source in American broccoli ( Brassica oleracea ) genotypes

Alexandre A. Degrave , Marine Wagner , Pierre George , Laurent Coudard , Xavier Pinochet
Molecular Plant Pathology, 2021, 22 (12), pp.1599-1612. ⟨10.1111/mpp.13131⟩
Article dans une revue hal-03344127v1

Apoplastic effector proteins of plant-associated fungi and oomycetes

Mercedes Rocafort , Isabelle Fudal-Grolier Fudal , Carl Mesarich
Current Opinion in Plant Biology, 2020, 56, pp.9-19. ⟨10.1016/j.pbi.2020.02.004⟩
Article dans une revue hal-02894839v1

A two genes – for – one gene interaction between Leptosphaeria maculans and Brassica napus

Yohann Petit-Houdenot , Alexandre A. Degrave , Michel Meyer , Françoise Blaise , Bénédicte Ollivier
New Phytologist, 2019, 223 (1), pp.397-411. ⟨10.1111/nph.15762⟩
Article dans une revue hal-02278749v1

Effector Biology in Fungal Pathogens of Nonmodel Crop Plants

Isabelle Fudal , Marie-Helene Balesdent , Thierry Rouxel
Trends in Plant Science, 2018, 23 (9), pp.753 - +. ⟨10.1016/j.tplants.2018.07.002⟩
Article dans une revue hal-02626782v1

The genome biology of effector gene evolution in filamentous plant pathogens

Andrea Sanchez-Vallet , Simone Fouche , Isabelle Fudal , Fanny E. Hartmann , Jessica L Soyer
Annual Review of Phytopathology, 2018, 56, ⟨10.1146/annurev-phyto-080516-035303⟩
Article dans une revue hal-02628719v1
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Different waves of effector genes with contrasted genomic location are expressed by Leptosphaeria maculans during cotyledon and stem colonization of oilseed rape

Julie Gervais , Clémence Plissonneau , Juliette Linglin , Michel Meyer , Karine Labadie
Molecular Plant Pathology, 2017, 18 (8), pp.1113 - 1126. ⟨10.1111/mpp.12464⟩
Article dans une revue hal-01608614v1
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Complex Interactions between Fungal Avirulence Genes and Their Corresponding Plant Resistance Genes and Consequences for Disease Resistance Management

Yohann Petit , Isabelle Fudal
Frontiers in Plant Science, 2017, 8, ⟨10.3389/fpls.2017.01072⟩
Article dans une revue hal-01602583v1
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A game of hide and seek between avirulence genes AvrLm4‐7 and AvrLm3 in Leptosphaeria maculans

Clémence Plissonneau , Guillaume Daverdin , Bénédicte Ollivier , Françoise Blaise , Alexandre A. Degrave
New Phytologist, 2016, 209 (4), pp.1613-1624. ⟨10.1111/nph.13736⟩
Article dans une revue hal-02516669v1
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The N-end rule pathway regulates pathogen responses in plants

Rémi de Marchi , Maud Sorel , Brian Mooney , Isabelle Fudal-Grolier Fudal , Kevin Goslin
Scientific Reports, 2016, 6 (1), ⟨10.1038/srep26020⟩
Article dans une revue hal-02104524v1

The APSES transcription factor LmStuA is required for sporulation, pathogenic development and effector gene expression in Leptosphaeria maculans

Jessica Louise Soyer , Audrey Hamiot , Benedicte Ollivier , Marie-Helene Balesdent , Thierry Rouxel
Molecular Plant Pathology, 2015, 16 (9), pp.1000-1005. ⟨10.1111/mpp.12249⟩
Article dans une revue hal-01536499v1

Rapid identification of the Leptosphaeria maculans avirulence gene AvrLm2 using an intraspecific comparative genomics approach

Kaveh Ghanbarnia , Isabelle Fudal , Nicholas J. Larkan , Matthew G. Links , Marie-Helene Balesdent
Molecular Plant Pathology, 2015, 16 (7), pp.699 - 709. ⟨10.1111/mpp.12228⟩
Article dans une revue hal-01536462v1

Chromatin-based control of effector gene expression in plant-associated fungi

Jessica L. Soyer , Thierry T. Rouxel , Isabelle Fudal-Grolier Fudal
Current Opinion in Plant Biology, 2015, 26, pp.51-56. ⟨10.1016/j.pbi.2015.05.025⟩
Article dans une revue hal-02638628v1

Crystal structure of the effector AvrLm4-7 of Leptosphaeria maculans reveals insights into its translocation into plant cells and recognition by resistance proteins.

Karine Blondeau , Françoise Blaise , Marc Graille , Shiv D Kale , Juliette Linglin
Plant Journal, 2015, 83 (4), pp.610-24. ⟨10.1111/tpj.12913⟩
Article dans une revue hal-01446655v1

Quantitative resistance affects the speed of frequency increase but not the diversity of the virulence alleles overcoming a major resistance gene to [i]Leptosphaeria maculans[/i] in oilseed rape

Régine Delourme , Lydia Bousset , Magali Ermel , Philippe Duffe , Anne-Laure Besnard
Infection, Genetics and Evolution, 2014, 27, pp.490-499. ⟨10.1016/j.meegid.2013.12.019⟩
Article dans une revue hal-01208690v1
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Epigenetic Control of Effector Gene Expression in the Plant Pathogenic Fungus [i]Leptosphaeria maculans[/i]

Jessica Soyer , Mennat El Ghalid , Nicolas Glaser , Bénédicte Ollivier , Juliette Linglin
PLoS Genetics, 2014, 10 (3), 19 p. ⟨10.1371/journal.pgen.1004227⟩
Article dans une revue hal-01019254v1
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Transposable element-assisted evolution and adaptation to host plant within the Leptosphaeria maculans-Leptosphaeria biglobosa species complex of fungal pathogens

Jonathan J. Grandaubert , Rohan G.T. Lowe , Jessica L. Soyer , C.L. Schoch , Angela P van de Wouw
BMC Genomics, 2014, 15 (891), pp.OA. ⟨10.1186/1471-2164-15-891⟩
Article dans une revue hal-02631920v1
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Microbe-Independent Entry of Oomycete RxLR Effectors and Fungal RxLR-Like Effectors Into Plant and Animal Cells Is Specific and Reproducible

Brett M. B. M. Tyler , Shiv D. S. D. Kale , Qunqing Q. Wang , Kai K. Tao , Helen R. H. R. Clark
Molecular Plant-Microbe Interactions, 2013, 26 (6), pp.611 - 616. ⟨10.1094/mpmi-02-13-0051-ia⟩
Article dans une revue hal-01001070v1
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The dispensable chromosome of Leptosphaeria maculans shelters an effector gene conferring avirulence towards Brassica rapa.

Marie-Helene Balesdent , Isabelle Fudal-Grolier Fudal , Bénédicte B. Ollivier , Pascal P. Bally , Jonathan J. Grandaubert
New Phytologist, 2013, 198 (3), pp.887-898. ⟨10.1111/nph.12178⟩
Article dans une revue hal-01004245v1
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Genome Structure and Reproductive Behaviour Influence the Evolutionary Potential of a Fungal Phytopathogen

Guillaume Daverdin , Thierry Rouxel , Lilian Gout , Jean-Noel Aubertot , Isabelle Fudal
PLoS Pathogens, 2012, 8 (11), 15 p. ⟨10.1371/journal.ppat.1003020⟩
Article dans une revue hal-01019027v1
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Incidence of Genome Structure, DNA Asymmetry, and Cell Physiology on T-DNA Integration in Chromosomes of the Phytopathogenic Fungus Leptosphaeria maculans

Ahmed Salim A. S. Bourras , Michel M. Meyer , Jonathan J. Grandaubert , Nicolas Lapalu , Isabelle Fudal-Grolier Fudal
G3, 2012, 2 (8), pp.891-904. ⟨10.1534/g3.112.002048⟩
Article dans une revue hal-01000638v1

External lipid P13P mediates entry of eukaryotic pathogen effectors into plant and animal host cells

Shiv D. Kale , Biao Gu , Daniel G.S. Capelluto , Daolong Dou , Emily Feldman
Cell, 2010, 142 (2), pp.284-295. ⟨10.1016/j.cell.2010.06.008⟩
Article dans une revue hal-02667928v1

Repeat-induced point mutation (RIP) as an alternative mechanism of evolution toward virulence in Leptosphaeria maculans

Isabelle Fudal-Grolier Fudal , Simon Ross , Hortense H. Brun , Anne-Laure Besnard , Magali M. Ermel
Molecular Plant-Microbe Interactions, 2009, 22 (8), pp.932-941. ⟨10.1094/MPMI-22-8-0932⟩
Article dans une revue hal-02667764v1

Leptosphaeria maculans avirulence gene AvrLm4-7 confers a dual recognition specificity by the Rlm4 and Rlm7 resistance genes of oilseed rape and circumvents Rlm4-mediated recognition through a single amino acid change

Francis Parlange , Guillaume G. Daverdin , Marie-Line Kuhn , Isabelle Fudal-Grolier Fudal , Marie-Helene Balesdent
Molecular Microbiology, 2009, 71 (4), pp.851-863. ⟨10.1111/j.1365-2958.2008.06547.x⟩
Article dans une revue hal-02663864v1

Magnaporhe grisea avirulence gene ACE1 belons to an infection-specific gene cluster involved in secondary metabolism

Jérôme Collemare , M. Pianfetti , M. Houllé , A.E. Morin , Laurent Camborde
New Phytologist, 2008, 179 (1), pp.196-208. ⟨10.1111/j.1469-8137.2008.02459.x⟩
Article dans une revue hal-02669102v1

Heterochromatin-like regions as ecological niches for avirulence genes in the Leptosphaeria maculans genome : map-based cloning of AvrLm6

Isabelle Fudal-Grolier Fudal , S Ross , Lilian L. Gout , Francoise F. Blaise , Marie-Line Kuhn
Molecular Plant-Microbe Interactions, 2007, 20 (4), pp.459-470. ⟨10.1094/MPMI-20-4-0459⟩
Article dans une revue hal-02656853v1

Expression of Magnaporthe grisea Avirulence Gene ACE1 Is Connected to the Initiation of Appressorium-Mediated Penetration

Isabelle Fudal , Jérôme Collemare , Heidi U. Böhnert , Delphine Melayah , Marc-Henri Lebrun
Eukaryotic Cell, 2007, 6 (3), pp.546-554. ⟨10.1128/EC.00330-05⟩
Article dans une revue halsde-00180977v1

Lost in the middle of nowhere: the AvrLm1 avirulence gene of the dothideomycete Leptosphaeria maculans

Lilian L. Gout , Isabelle Fudal-Grolier Fudal , Marie-Line Kuhn , Francoise F. Blaise , Maria Eckert
Molecular Microbiology, 2006, 60 (1), pp.67-80. ⟨10.1111/j.1365-2958.2006.05076.x⟩
Article dans une revue hal-02662679v1

Origins of host-specific populations of the blast pathogen Magnaporthe oryzae in crop domestication with subsequent expansion of pandemic clones on rice and weeds of rice

Brett C Couch , Isabelle Fudal , Marc-Henri M.-H. Lebrun , Didier Tharreau , Barbara Valent
Genetics, 2005, 170 (2), pp.613-630. ⟨10.1534/genetics.105.041780⟩
Article dans une revue hal-02681928v1
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Epigenetic regulation of fungal genes involved in plant colonization

Jessica L. Soyer , Isabelle Fudal
The Mycota, Plant Relationships / Fungal – plant interactions., 5, springer, pp.255-281, 2023, ⟨10.1007/978-3-031-16503-0⟩
Chapitre d'ouvrage hal-04432844v1