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Personal page of Stephanie Heux

Stephanie HEUX  is team leader at Toulouse Biotechnology  Institute (TBI, former LISBP).   She has more than 15 years of experience with microorganisms as a model, metabolic engineering and NMR & MS-based metabolomics and fluxomics. After 2 years of Marie Curie fellowship at ETH Zurich where she developed advanced metabolomics methods and data mining approaches, she joined the TBI as INRA researcher to undertake systems biotechnology research. She is interested in the microbial metabolism and, in particular, its organization, its functioning and its adaptation. She applied systemic, iterative, integrative approaches to understand and predict the behavior of the natural and synthetic metabolic systems of various microorganisms for biotechnological applications.  To do that, she developed in silico (reconstruction and modelling of the metabolism) and experimental methods (metabolomics, fluxomics).

Journal articles28 documents

  • Cécilia Bergès, Edern Cahoreau, Pierre Millard, Brice Enjalbert, Mickael Dinclaux, et al.. Exploring the Glucose Fluxotype of the E. coli y-ome Using High-Resolution Fluxomics. Metabolites, MDPI, 2021, 11 (5), pp.271. ⟨10.3390/metabo11050271⟩. ⟨hal-03216838⟩
  • Angeline Pelus, Gaëlle Bordes, Sophie Barbe, Younes Bouchiba, Callum Burnard, et al.. A tripartite carbohydrate-binding module to functionalize cellulose nanocrystal. Biomaterials Science, Royal Society of Chemistry (RSC), 2021, 9 (22), pp.7444-7455. ⟨10.1039/d1bm01156a⟩. ⟨hal-03372120⟩
  • Luciana Brito, Marta Irla, Ingemar Nærdal, Simone Le, Baudoin Delépine, et al.. Evaluation of Heterologous Biosynthetic Pathways for Methanol-Based 5-Aminovalerate Production by Thermophilic Bacillus methanolicus. Frontiers in Bioengineering and Biotechnology, Frontiers, 2021, 9, ⟨10.3389/fbioe.2021.686319⟩. ⟨hal-03437024⟩
  • Guido Hennig, Carsten Haupka, Luciana Brito, Christian Rückert, Edern Cahoreau, et al.. Methanol-Essential Growth of Corynebacterium glutamicum: Adaptive Laboratory Evolution Overcomes Limitation due to Methanethiol Assimilation Pathway. International Journal of Molecular Sciences, MDPI, 2020, 21 (10), ⟨10.3390/ijms21103617⟩. ⟨hal-02906365⟩
  • Baudoin Delépine, Marina Gil López, Marc Carnicer, Cláudia Vicente, Volker Wendisch, et al.. Charting the Metabolic Landscape of the Facultative Methylotroph Bacillus methanolicus. mSystems, 2020, 5 (5), ⟨10.1128/mSystems.00745-20⟩. ⟨hal-02960832⟩
  • Carsten Haupka, Baudoin Delépine, Marta Irla, Stephanie Heux, Volker Wendisch. Flux Enforcement for Fermentative Production of 5-Aminovalerate and Glutarate by Corynebacterium glutamicum. Catalysts, MDPI, 2020, 10 (9), ⟨10.3390/catal10091065⟩. ⟨hal-02960837⟩
  • Alessandro de Simone, Cláudia M Vicente, Camille Peiro, Lara Gales, Floriant Bellvert, et al.. Mixing and matching methylotrophic enzymes to design a novel methanol utilization pathway in E. coli. Metabolic Engineering, Elsevier, 2020, 61, pp.315-325. ⟨10.1016/j.ymben.2020.07.005⟩. ⟨hal-02935411⟩
  • Pierre Millard, Uwe Schmitt, Patrick Kiefer, Julia A. Vorholt, Stephanie Heux, et al.. ScalaFlux: a scalable approach to quantify fluxes in metabolic subnetworks. PLoS Computational Biology, Public Library of Science, 2020, 16 (4), ⟨10.1101/735308⟩. ⟨hal-02306115⟩
  • Sara Castaño-Cerezo, Hanna Kulyk-Barbier, Pierre Millard, Jean-Charles Portais, Stephanie Heux, et al.. Functional analysis of isoprenoid precursors biosynthesis by quantitative metabolomics and isotopologue profiling. Metabolomics, Springer Verlag, 2019, 15 (9), 10 p. ⟨10.1007/s11306-019-1580-8⟩. ⟨hal-02269068⟩
  • Camille Peiro, Pierre Millard, Alessandro de Simone, Edern Cahoreau, Lindsay Peyriga, et al.. Chemical and Metabolic Controls on Dihydroxyacetone Metabolism Lead to Suboptimal Growth of Escherichia coli. Applied and Environmental Microbiology, American Society for Microbiology, 2019, 85 (15), pp.1-17. ⟨10.1128/AEM.00768-19⟩. ⟨hal-02194204⟩
  • Ludovic Cottret, Clément Frainay, Maxime Chazalviel, Floréal Cabanettes, Yoann Gloaguen, et al.. MetExplore: collaborative edition and exploration of metabolic networks. Nucleic Acids Research, Oxford University Press, 2018, 46 (W1), 8 p. ⟨10.1093/nar/gky301⟩. ⟨hal-01886470⟩
  • Débora Trichez, Clément Auriol, Audrey Baylac, Romain Irague, Clémentine Dressaire, et al.. Engineering of Escherichia coli for Krebs cycle-dependent production of malic acid. Microbial Cell Factories, BioMed Central, 2018, 17 (1), 12 p. ⟨10.1186/s12934-018-0959-y⟩. ⟨hal-01886486⟩
  • Stephanie Heux, Cécilia Berges, Pierre Millard, Jean-Charles Portais, Fabien Letisse. Recent advances in high-throughput 13C-fluxomics. Current Opinion in Biotechnology, Elsevier, 2017, 43, pp.104-109. ⟨10.1016/j.copbio.2016.10.010⟩. ⟨hal-01604972⟩
  • Alexandra Tauzin, Elisabeth Laville, Yao Xiao, Sebastien Nouaille, Pascal Le Bourgeois, et al.. Functional characterization of a gene locus from an uncultured gut Bacteroides conferring xylo-oligosaccharides utilization to Escherichia coli. Molecular Microbiology, Wiley, 2016, 102 (4), pp.579 - 592. ⟨10.1111/mmi.13480⟩. ⟨hal-01886420⟩
  • Marc Carnicer, Gilles Vieira, Trygve Brautaset, Jean-Charles Portais, Stephanie Heux. Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus. Microbial Cell Factories, BioMed Central, 2016, 15 (1), pp.92. ⟨10.1186/s12934-016-0483-x⟩. ⟨hal-02129756⟩
  • Stephanie Heux, Isabelle Meynial Salles, Michael O'Donohue, Claire Dumon. White biotechnology: state of the art strategies for the development of biocatalysts for biorefining. Biotechnology Advances, Elsevier, 2015, 33 (8), ⟨10.1016/j.biotechadv.2015.08.004⟩. ⟨hal-01269233⟩
  • J. E. Muller, F. Meyer, B. Litsanov, P. Kiefer, E. Potthoff, et al.. Engineering Escherichia coli for methanol conversion. Metabolic Engineering, Elsevier, 2015, 28, pp.190-201. ⟨10.1016/j.ymben.2014.12.008⟩. ⟨hal-01269203⟩
  • L. Lessmeier, J. Pfeifenschneider, Marc Carnicer Heras, Stephanie Heux, Jean-Charles Portais, et al.. Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13-labeled methanol as co-substrate. Applied Microbiology and Biotechnology, Springer Verlag, 2015, 99 (23), ⟨10.1007/s00253-015-6906-5⟩. ⟨hal-01269204⟩
  • Gilles Vieira, Marc Carnicer Heras, Jean-Charles Portais, Stephanie Heux. FindPath: a Matlab solution for in silico design of synthetic metabolic pathways. Bioinformatics, Oxford University Press (OUP), 2014, 30 (20), pp.2986-2988. ⟨10.1093/bioinformatics/btu422⟩. ⟨hal-01268971⟩
  • Stephanie Heux, Juliette Poinot, Stéphane Massou, Serguei Sokol, Jean-Charles Portais. A novel platform for automated high-throughput fluxome profiling of metabolic variants. Metabolic Engineering, Elsevier, 2014, 25, pp.8 - 19. ⟨10.1016/j.ymben.2014.06.001⟩. ⟨hal-01268982⟩
  • Stephanie Heux, Thomas Fuchs, Joachim Buhmann, Nicola Zamboni, Uwe Sauer. A high-throughput metabolomics method to predict high concentration cytotoxicity of drugs from low concentration profiles. Metabolomics, Springer Verlag, 2012, 8 (3), pp.433-443. ⟨10.1007/s11306-011-0386-0⟩. ⟨hal-02129766⟩
  • Stephanie Heux, Benjamin Philippe, Jean-Charles Portais. High-Throughput Workflow for Monitoring and Mining Bioprocess Data and Its Application to Inferring the Physiological Response of Escherichia coli to Perturbations. Applied and Environmental Microbiology, American Society for Microbiology, 2011, 77 (19), pp.7040-7049. ⟨10.1128/AEM.05838-11⟩. ⟨hal-02129764⟩
  • Jennifer Christina Ewald, Stephanie Heux, Nicola Zamboni. High-Throughput Quantitative Metabolomics: Workflow for Cultivation, Quenching, and Analysis of Yeast in a Multiwell Format. Analytical Chemistry, American Chemical Society, 2009, 81 (9), pp.3623-3629. ⟨10.1021/ac900002u⟩. ⟨hal-02129771⟩
  • Stephanie Heux, Axelle Cadière, Sylvie Dequin. Glucose utilization of strains lacking PGI1 and expressing a transhydrogenase suggests differences in the pentose phosphate capacity among Saccharomyces cerevisiae strains. FEMS Yeast Research, Oxford University Press (OUP), 2008, 8 (2), pp.217-224. ⟨10.1111/j.1567-1364.2007.00330.x⟩. ⟨hal-02129773⟩
  • Stephanie Heux, J.-M. Sablayrolles, Rémy Cachon, Sylvie Dequin. Engineering a Saccharomyces cerevisiae Wine Yeast That Exhibits Reduced Ethanol Production during Fermentation under Controlled Microoxygenation Conditions. Applied and Environmental Microbiology, American Society for Microbiology, 2006, 72 (9), pp.5822-5828. ⟨10.1128/AEM.00750-06⟩. ⟨hal-02129781⟩
  • Stephanie Heux, Rémy Cachon, Sylvie Dequin. Cofactor engineering in Saccharomyces cerevisiae: Expression of a H2O-forming NADH oxidase and impact on redox metabolism. Metabolic Engineering, Elsevier, 2006, 8 (4), pp.303-314. ⟨10.1016/j.ymben.2005.12.003⟩. ⟨hal-02129775⟩
  • Stephanie Heux, Fabien Morin, Rod A. Lea, Micky Ovcaric, Lofti Tajouri, et al.. The Methylentetrahydrofolate Reductase Gene Variant (C677T) as a Risk Factor for Essential Hypertension in Caucasians. Hypertension Research, Nature Publishing Group, 2004, 27 (9), pp.663-667. ⟨10.1291/hypres.27.663⟩. ⟨hal-02129844⟩
  • Kirsty Wright, Lotti Tajouri, Rod Lea, Micky Ovcaric, Stephanie Heux, et al.. The role of adenosine-related genes variants in susceptibility to essential hypertension. Journal of Hypertension, Lippincott, Williams & Wilkins, 2004, 22 (8), pp.1519-1522. ⟨10.1097/01.hjh.0000133723.16947.6d⟩. ⟨hal-02129847⟩

Book sections1 document

  • Stephanie Heux, Trygve Brautaset, Julia A. Vorholt, Volker F. Wendisch, Jean-Charles Portais. Synthetic methylotrophy: past, present, and future. Methane Biocatalysis: Paving the Way to Sustainability, Editions Springer, 2018, 978-3-319-74865-8. ⟨hal-02154351⟩

Habilitation à diriger des recherches1 document

  • Stephanie Heux. Understand and optimize natural and synthetic metabolic networks of microorganisms. Life Sciences [q-bio]. Université Toulouse III Paul Sabatier, 2019. ⟨tel-02961067⟩