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Stephanie Heux

36
Documents

Présentation

**Stephanie HEUX** is team leader at Toulouse Biotechnology Institute (TBI, former LISBP). She has more than 15 years of experience with microorganisms as a model, metabolic engineering and NMR & MS-based metabolomics and fluxomics. After 2 years of Marie Curie fellowship at ETH Zurich where she developed advanced metabolomics methods and data mining approaches, she joined the TBI as INRA researcher to undertake systems biotechnology research. She is interested in the microbial metabolism and, in particular, its organization, its functioning and its adaptation. She applied systemic, iterative, integrative approaches to understand and predict the behavior of the natural and synthetic metabolic systems of various microorganisms for biotechnological applications. To do that, she developed *in silico* (reconstruction and modelling of the metabolism) and experimental methods (metabolomics, fluxomics).

Publications

PhysioFit: a software to quantify cell growth parameters and extracellular fluxes

Loïc Le Grégam , Yann Guitton , Floriant Bellvert , Stéphanie Heux , Fabien Jourdan
bioRxiv : n'est pas une revue mais un serveur de preprint, 2023, ⟨10.1101/2023.10.12.561695⟩
Article dans une revue hal-04253692v1
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High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux

Albert Fina , Pierre Millard , Joan Albiol , Pau Ferrer , Stephanie Heux
Microbial Cell Factories, 2023, 22 (1), pp.1-16. ⟨10.1186/s12934-023-02123-0⟩
Article dans une revue hal-04170885v1
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Combining Metabolic Engineering and Multiplexed Screening Methods for 3-Hydroxypropionic Acid Production in Pichia pastoris

Albert Fina , Stephanie Heux , Joan Albiol , Pau Ferrer
Frontiers in Bioengineering and Biotechnology, 2022, 10, ⟨10.3389/fbioe.2022.942304⟩
Article dans une revue hal-03836687v1

From a Hetero- to a Methylotrophic Lifestyle: Flash Back on the Engineering Strategies to Create Synthetic Methanol-User Strains

Camille Peiro , Cláudia M Vicente , Denis Jallet , Stephanie Heux
Frontiers in Bioengineering and Biotechnology, 2022, 10, ⟨10.3389/fbioe.2022.907861⟩
Article dans une revue hal-03800652v1
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Exploring the Glucose Fluxotype of the E. coli y-ome Using High-Resolution Fluxomics

Cécilia Bergès , Edern Cahoreau , Pierre Millard , Brice Enjalbert , Mickael Dinclaux
Metabolites, 2021, 11 (5), pp.271. ⟨10.3390/metabo11050271⟩
Article dans une revue hal-03216838v1
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A tripartite carbohydrate-binding module to functionalize cellulose nanocrystal

Angeline Pelus , Gaëlle Bordes , Sophie Barbe , Younes Bouchiba , Callum Burnard
Biomaterials Science, 2021, 9 (22), pp.7444-7455. ⟨10.1039/d1bm01156a⟩
Article dans une revue hal-03372120v1
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Evaluation of Heterologous Biosynthetic Pathways for Methanol-Based 5-Aminovalerate Production by Thermophilic Bacillus methanolicus

Luciana Fernandes Brito , Marta Irla , Ingemar Nærdal , Simone Balzer Le , Baudoin Delépine
Frontiers in Bioengineering and Biotechnology, 2021, 9, ⟨10.3389/fbioe.2021.686319⟩
Article dans une revue hal-03437024v1
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Charting the Metabolic Landscape of the Facultative Methylotroph Bacillus methanolicus

Baudoin Delépine , Marina Gil López , Marc Carnicer , Cláudia Vicente , Volker Wendisch
mSystems, 2020, 5 (5), ⟨10.1128/mSystems.00745-20⟩
Article dans une revue hal-02960832v1

Flux Enforcement for Fermentative Production of 5-Aminovalerate and Glutarate by Corynebacterium glutamicum

Carsten Haupka , Baudoin Delépine , Marta Irla , Stephanie Heux , Volker F Wendisch
Catalysts, 2020, 10 (9), pp.1065. ⟨10.3390/catal10091065⟩
Article dans une revue hal-02960837v1
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Methanol-Essential Growth of Corynebacterium glutamicum: Adaptive Laboratory Evolution Overcomes Limitation due to Methanethiol Assimilation Pathway

Guido Hennig , Carsten Haupka , Luciana F Brito , Christian Rückert , Edern Cahoreau
International Journal of Molecular Sciences, 2020, 21 (10), pp.3617. ⟨10.3390/ijms21103617⟩
Article dans une revue hal-02906365v1
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ScalaFlux: a scalable approach to quantify fluxes in metabolic subnetworks

Pierre Millard , Uwe Schmitt , Patrick Kiefer , Julia A. Vorholt , Stephanie Heux
PLoS Computational Biology, 2020, 16 (4), ⟨10.1101/735308⟩
Article dans une revue hal-02306115v1
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Mixing and matching methylotrophic enzymes to design a novel methanol utilization pathway in E. coli

Alessandro de Simone , Cláudia M Vicente , Camille Peiro , Lara Gales , Floriant Bellvert
Metabolic Engineering, 2020, 61, pp.315-325. ⟨10.1016/j.ymben.2020.07.005⟩
Article dans une revue hal-02935411v1
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Chemical and Metabolic Controls on Dihydroxyacetone Metabolism Lead to Suboptimal Growth of Escherichia coli

Camille Peiro , Pierre Millard , Alessandro de Simone , Edern Cahoreau , Lindsay Peyriga
Applied and Environmental Microbiology, 2019, 85 (15), pp.1-17. ⟨10.1128/AEM.00768-19⟩
Article dans une revue hal-02194204v1

Functional analysis of isoprenoid precursors biosynthesis by quantitative metabolomics and isotopologue profiling

Sara Castaño-Cerezo , Hanna Kulyk-Barbier , Pierre Millard , Jean-Charles Portais , Stephanie Heux
Metabolomics, 2019, 15 (9), 10 p. ⟨10.1007/s11306-019-1580-8⟩
Article dans une revue hal-02269068v1
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MetExplore: collaborative edition and exploration of metabolic networks

Ludovic Cottret , Clément Frainay , Maxime Chazalviel , Floréal Cabanettes , Yoann Gloaguen
Nucleic Acids Research, 2018, 46 (W1), 8 p. ⟨10.1093/nar/gky301⟩
Article dans une revue hal-01886470v1
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Engineering of Escherichia coli for Krebs cycle-dependent production of malic acid

Débora Trichez , Clément Auriol , Audrey Baylac , Romain Irague , Clémentine Dressaire
Microbial Cell Factories, 2018, 17 (1), 12 p. ⟨10.1186/s12934-018-0959-y⟩
Article dans une revue hal-01886486v1
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Recent advances in high-throughput 13C-fluxomics

Stephanie Heux , Cécilia Berges , Pierre Millard , Jean-Charles Portais , Fabien Letisse
Current Opinion in Biotechnology, 2017, 43, pp.104-109. ⟨10.1016/j.copbio.2016.10.010⟩
Article dans une revue hal-01604972v1
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Functional characterization of a gene locus from an uncultured gut Bacteroides conferring xylo-oligosaccharides utilization to Escherichia coli

Alexandra Tauzin , Elisabeth Laville , Yao Xiao , Sébastien Nouaille , Pascal Le Bourgeois
Molecular Microbiology, 2016, 102 (4), pp.579 - 592. ⟨10.1111/mmi.13480⟩
Article dans une revue hal-01886420v1
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Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus

Marc Carnicer , Gilles Vieira , Trygve Brautaset , Jean-Charles Portais , Stephanie Heux
Microbial Cell Factories, 2016, 15 (1), pp.92. ⟨10.1186/s12934-016-0483-x⟩
Article dans une revue hal-02129756v1
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Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13-labeled methanol as co-substrate

L. Lessmeier , J. Pfeifenschneider , Marc Carnicer Heras , Stephanie Heux , Jean-Charles Portais
Applied Microbiology and Biotechnology, 2015, 99 (23), ⟨10.1007/s00253-015-6906-5⟩
Article dans une revue hal-01269204v1

White biotechnology: state of the art strategies for the development of biocatalysts for biorefining

Stephanie Heux , Isabelle Meynial Salles , Michael O'Donohue , Claire Dumon
Biotechnology Advances, 2015, 33 (8), ⟨10.1016/j.biotechadv.2015.08.004⟩
Article dans une revue hal-01269233v1
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Engineering Escherichia coli for methanol conversion

J. E. Muller , F. Meyer , B. Litsanov , P. Kiefer , E. Potthoff
Metabolic Engineering, 2015, 28, pp.190-201. ⟨10.1016/j.ymben.2014.12.008⟩
Article dans une revue hal-01269203v1
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A novel platform for automated high-throughput fluxome profiling of metabolic variants

Stephanie Heux , Juliette Poinot , Stéphane Massou , Serguei Sokol , Jean-Charles Portais
Metabolic Engineering, 2014, 25, pp.8 - 19. ⟨10.1016/j.ymben.2014.06.001⟩
Article dans une revue hal-01268982v1
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FindPath: a Matlab solution for in silico design of synthetic metabolic pathways

Gilles Vieira , Marc Carnicer Heras , Jean-Charles Portais , Stephanie Heux
Bioinformatics, 2014, 30 (20), pp.2986-2988. ⟨10.1093/bioinformatics/btu422⟩
Article dans une revue hal-01268971v1

A high-throughput metabolomics method to predict high concentration cytotoxicity of drugs from low concentration profiles

Stephanie Heux , Thomas Fuchs , Joachim M Buhmann , Nicola Zamboni , Uwe Sauer
Metabolomics, 2012, 8 (3), pp.433-443. ⟨10.1007/s11306-011-0386-0⟩
Article dans une revue hal-02129766v1
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High-Throughput Workflow for Monitoring and Mining Bioprocess Data and Its Application to Inferring the Physiological Response of Escherichia coli to Perturbations

Stephanie Heux , Benjamin Philippe , Jean-Charles Portais
Applied and Environmental Microbiology, 2011, 77 (19), pp.7040-7049. ⟨10.1128/AEM.05838-11⟩
Article dans une revue hal-02129764v1
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High-Throughput Quantitative Metabolomics: Workflow for Cultivation, Quenching, and Analysis of Yeast in a Multiwell Format

Jennifer Christina Ewald , Stephanie Heux , Nicola Zamboni
Analytical Chemistry, 2009, 81 (9), pp.3623-3629. ⟨10.1021/ac900002u⟩
Article dans une revue hal-02129771v1
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Glucose utilization of strains lacking PGI1 and expressing a transhydrogenase suggests differences in the pentose phosphate capacity among Saccharomyces cerevisiae strains

Stephanie Heux , Axelle Cadière , Sylvie Dequin
FEMS Yeast Research, 2008, 8 (2), pp.217-224. ⟨10.1111/j.1567-1364.2007.00330.x⟩
Article dans une revue hal-02129773v1
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Engineering a Saccharomyces cerevisiae Wine Yeast That Exhibits Reduced Ethanol Production during Fermentation under Controlled Microoxygenation Conditions

Stephanie Heux , J.-M. Sablayrolles , Rémy Cachon , Sylvie Dequin
Applied and Environmental Microbiology, 2006, 72 (9), pp.5822-5828. ⟨10.1128/AEM.00750-06⟩
Article dans une revue hal-02129781v1
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Cofactor engineering in Saccharomyces cerevisiae: Expression of a H2O-forming NADH oxidase and impact on redox metabolism

Stephanie Heux , Rémy Cachon , Sylvie Dequin
Metabolic Engineering, 2006, 8 (4), pp.303-314. ⟨10.1016/j.ymben.2005.12.003⟩
Article dans une revue hal-02129775v1
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The role of adenosine-related genes variants in susceptibility to essential hypertension

Kirsty Wright , Lotti Tajouri , Rod Lea , Micky Ovcaric , Stephanie Heux
Journal of Hypertension, 2004, 22 (8), pp.1519-1522. ⟨10.1097/01.hjh.0000133723.16947.6d⟩
Article dans une revue hal-02129847v1
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The Methylentetrahydrofolate Reductase Gene Variant (C677T) as a Risk Factor for Essential Hypertension in Caucasians

Stephanie Heux , Fabien Morin , Rod A. Lea , Micky Ovcaric , Lofti Tajouri
Hypertension Research, 2004, 27 (9), pp.663-667. ⟨10.1291/hypres.27.663⟩
Article dans une revue hal-02129844v1

Aerobic Utilization of Methanol for Microbial Growth and Production

Volker Wendisch , Gregor Kosec , Stephanie Heux , Trygve Brautaset
One-Carbon Feedstocks for Sustainable Bioproduction, 180, Springer International Publishing, pp.169-212, 2022, Advances in Biochemical Engineering/Biotechnology, 978-3-031-06854-6. ⟨10.1007/10_2021_177⟩
Chapitre d'ouvrage hal-03980092v1
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Synthetic methylotrophy: past, present, and future

Stephanie Heux , Trygve Brautaset , Julia A. Vorholt , Volker F. Wendisch , Jean-Charles Portais
Methane Biocatalysis: Paving the Way to Sustainability, Editions Springer, 2018, 978-3-319-74865-8
Chapitre d'ouvrage hal-02154351v1