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CV Hervé LE HIR


 

CURRENT POSITION           Research Director, 1st class - CNRS

 

                                               Head of IBENS section "Functional Genomics"

 

                                               Group leader, team "Expression of eukaryotic mRNAs"

 

                                               Institut de Biologie de l’Ecole Normale Supérieure (IBENS)

 

                                               UMR8197-U1024. 46 rue d’Ulm, 75005, Paris

 

 

 

EDUCATION             2006 : HDR,  University Paris-Sud Orsay (Paris 11)

 

1998: PhD, Molecular Biology, University Paris Diderot (Paris 7)           

1993 : Master (B.A.) Biochemistry, UPMC (Paris 6)

 

 

 

DISTINCTION            2009 :ATIP and ATIP+ CNRS

 

                                   2010, 2014, 2019 : Prime d’Encadrement Doctoral et de Recherche CNRS

 

 

 

RESEARCH EXPERIENCE

 

       Since 2009:         Group leader of the team "Expression of eukaryotic mRNAs"

 

                   IBENS – Section 2, Functional Genomics – Paris

 

  2001-2008:          CR2/1 CNRS in the group of Dr. Bertrand Séraphin

 

                   CGM – CNRS UPR2167 – 91190 Gif-sur-Yvette, France.

 

  1998-2001:          Post-doctoral fellow in the lgroups of Dr. Lynne Maquat (Roswell Park Cancer Institute / Buffalo USA) and of Pr. Melissa J. Moore (HHMI / Brandeis University, MA. USA)

 

  1993-1998:          Ph.D. (PI: Dr. Claude Thermes) – University Paris 7

 

                                   CGM – CNRS UPR2167 – 91190 Gif-sur-Yvette, France.

 

FUNDING

 

2018-2021:      ANR blanche – spEJCificity (Project leader)

 

2015-2018:      ANR blanche – CleaNMD (Partner, coordinator Alain Jacquier)

 

2014-2017:      ANR blanche – DifferEJnCe (Project leader)

 

2014-2015:      PSL research – (Partner, coordinator Stephan Vagner)

 

2014-2015:      Association Recherche contre le Cancer – (Project leader)

 

2012-2015:      FP7 Marie Curie Initial Training Network « RNPnet »

 

2012-2013:      Fondation P.G. de Gennes (Partner)

 

2011-2013:      ANR blanche – EJCbirth (Project leader)

 

2010-2013:      Equipe FRM (Project leader)

 

2010:               Installation Nouvelle Equipe FRM

 

2010-2011:      Ligue Nationale Contre le Cancer (Project leader)     

 

2009-2011:      ANR blanche – Explore EJC (Project leader)

 

2009-2011:      ATIP CNRS

 

2005-2008:      ANR blanche – L’EJC (Project leader)

 

2003-2006:      A.C.I. Jeunes Chercheurs

 

2003-2004:      France-Berkeley Fund

 

Supervision activities

 

            1 Researcher (CR INSERM, J. Saulière)

 

6 PhD students (B. Marchadier, PE Chazal, R. Mishra, J. Kanaan, V. Busetto, J. Paternina)

 

5 Post-docs (C. Barrandon, F. Fiorini, Z. Wang, E. Marquenet, O.S. Kwon)

 

7 Engineers (F. Bonneau, V. Murigneux, L. Bastianelli, R. Servignat d’Aout, R. Hocq, Q.Alasseur, J. Paternina) 1 technician CNRS (I. Barbosa).

 

9 Master students

 

 

 

COMMUNICATIONS

 

Teaching activities

 

            Since 2009: Master in Genetics, UPMC (3h per year)

 

Since 2009: ENS L3 Chimie (2h per year)

 

Since 2009: ENS Master in Genetics, UPMC (3h per year)

 

2014: PhD program, Wellcome Trust Centre for Cell Biology, Edinburgh, UK (2014)

 

2014: PhD program, FMI, Basel, Switzerland (2014)

 

2012: PhD program, University Toulouse (2012)

 

2017: Co-organizer and teaching: International course Curie-ENS “Posttranscriptional

 

gene regulation” (2017)

 

Peer review and expertise activity

 

• Reviewer for several journals including: Cell; Nature; Nature Structural & Molecular Biology; Genes & Development; EMBO Journal; Journal of Cell Biology; Nucleic Acids Research; P.N.A.S.; Cell Report.

 

• Reviewer for several grant agencies including: ERC; Wellcome Trust; ANR; Ligue

 

contre le Cancer; Foreign agencies (Denmark, Poland, Israel, etc…).

 

• Since 2013: Member executive council doctoral school “Complexité du Vivant” – University UPMC (Paris 6).

 

• Since 2015: Member scientifc council “Ligue Grand Est”

 

• 2013-2016: Member scientifc council “Fondation Pierre-Gilles de Gennes”

 

• 2014: Vice-President ANR committee CES10

 

• 2016: Member ANR committee CES12            • 2017-2020: President ANR committee CES12

 

           

 

SELECTED PUBLICATIONS

 

 

Kanaan Hocq R, Paternina J, Alasseur Q, Genovesio A, Le Hir H. (2018) Monitored eCLIP: high accuracy mapping of RNA-protein interactions. Nucleic Acids Research 2018 Nov;46(21):11553-11565.

 

Kanaan J, Raj S, Decourty L, Saveanu C, Croquette V, Le Hir H. (2018) UPF1-like helicase grip on nucleic acids dictates processivity. Nature Communications. 2018 Sep;14(9):3752.

 

 

Le Hir H, Saulière J, Wang Z. (2016) The exon junction complex as a node of postranscriptional networks. Nature Review in Molecular and Cellular Biology. 2016 Jan;17(1):41-54.

 

Fiorini F, Bagchi D, Le Hir H*, Croquette V*. (2015) Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities. Nature Communications. Jul 3;6:7581. *Corresponding authors

 

Wang Z, Murigneux V, Le Hir H. (2014). Transcriptome-wide modulation of splicing by the Exon Junction Complex. Genome Biology. 15(12):551.

 

Chazal PE, Daguenet E, Wendling C, Ulrycke N, Tomasetto C, Sargueil B, Le Hir H (2013). EJC core component MLN51 interacts with eIF3 and activates translation. P.N.A.S. 110(15):5903-8.

 

Saulière J, Murigneux V, Wang Z, Marquenet E, Barbosa I, Le Tonquèze O, Audic Y, Paillard L, Roest Crollius H, Le Hir H. (2012). CLIP-seq of eIF4AIII reveals transcriptome-wide mapping of the human exon junction complex. Nature Structural and Molecular Biology 19(11):1124-31.

 

Barbosa I, Haque N, Fiorini F, Barrandon C, Tomasetto C, Blanchette M, Le Hir H. (2012). Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly. Nature Structural and Molecular Biology 19(10):983-90.

 

Chakrabarti S, Jayachandran U, Bonneau F, Fiorini F, Basquin C, Domcke S, Le Hir H, Conti E. (2011). Molecular Mechanisms for the RNA-Dependent ATPase Activity of Upf1 and Its Regulation by Upf2. Molecular Cell 41(6):682-692.

 

Saulière J, Haque N, Harms S, Barbosa I, Blanchette M, Le Hir H. (2010). The exon junction complex differentially marks spliced junctions. Nature Structural and Molecular Biology 17(10):1269-71.

 

Le Hir H, Séraphin B. (2008). EJCs at the heart of translational control. Cell. 133(2):213-6.

 

Chamieh H, Ballut L, Bonneau F, Le Hir H. (2008). NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity. Nature Structural and Molecular Biology 15(1):85-93.

 

Andersen CB*, Ballut L*, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Seraphin B, Le Hir H#, Andersen GR#. (2006). Structure of the Exon Junction Core Complex with a Trapped DEAD-Box ATPase Bound to RNA. Science 313:1968-1972.

 

Ballut L, Marchadier B, Baguet A, Tomasetto C, Séraphin B, Le Hir H. (2005). The Exon-Junction complex is locked onto mRNA by inhibition of eIF4A3 ATPase activity. Nature Structural and Molecular Biology 12(10):861-9.

 

Le Hir, H., Gatfield, D., Izaurralde, E. & Moore, M.J. (2001). The exon-exon junction complex provides a binding platform for factors involved in mRNA export and NMD. The EMBO Journal 20 (17):4987-4997.

 

Le Hir, H., Izaurralde, E., Maquat, L.E. & Moore, M.J. (2000). The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions. The EMBO Journal 19 (24):6860-6869.

 


Journal articles21 documents

  • Yuhui Dou, Isabelle Barbosa, Hua Jiang, Claudia Iasillo, Kelly Molloy, et al.. NCBP3 positively impacts mRNA biogenesis. Nucleic Acids Research, Oxford University Press, 2020, 48 (18), pp.10413-10427. ⟨10.1093/nar/gkaa744⟩. ⟨hal-03033173⟩
  • Virginia Busetto, Isabelle Barbosa, Jérôme Basquin, Emélie Marquenet, Rémi Hocq, et al.. Structural and functional insights into CWC27/CWC22 heterodimer linking the exon junction complex to spliceosomes. Nucleic Acids Research, Oxford University Press, 2020, 48 (10), pp.5670-5683. ⟨10.1093/nar/gkaa267⟩. ⟨pasteur-02862504⟩
  • Racha Chouaib, Adham Safieddine, Xavier Pichon, Arthur Imbert, Oh Sung Kwon, et al.. A Dual Protein-mRNA Localization Screen Reveals Compartmentalized Translation and Widespread Co-translational RNA Targeting. Developmental Cell, Elsevier, 2020, 54, pp.773 - 791.e5. ⟨10.1016/j.devcel.2020.07.010⟩. ⟨pasteur-02925687⟩
  • Mathieu Bahin, Benoit Noel, Valentine Murigneux, Charles Bernard, Leila Bastianelli, et al.. ALFA: annotation landscape for aligned reads. BMC Genomics, BioMed Central, 2019, 20 (250), pp.1-11. ⟨10.1186/s12864-019-5624-2⟩. ⟨hal-02084432⟩
  • Rémi Hocq, Janio Paternina, Quentin Alasseur, Auguste Genovesio, Hervé Le Hir. Monitored eCLIP: high accuracy mapping of RNA-protein interactions. Nucleic Acids Research, Oxford University Press, 2018, 46 (21), pp.11553-11565. ⟨10.1093/nar/gky858⟩. ⟨hal-02387433⟩
  • Marine Dehecq, Laurence Decourty, Abdelkader Namane, Caroline Proux, Joanne Kanaan, et al.. Nonsense‐mediated mRNA decay involves two distinct Upf1‐bound complexes. EMBO Journal, EMBO Press, 2018, 37, pp.e99278. ⟨10.15252/embj.201899278⟩. ⟨pasteur-01889054⟩
  • Joanne Kanaan, Saurabh Raj, Laurence Decourty, Cosmin Saveanu, Vincent Croquette, et al.. UPF1-like helicase grip on nucleic acids dictates processivity. Nature Communications, Nature Publishing Group, 2018, 9 (1), pp.3752. ⟨10.1038/s41467-018-06313-y⟩. ⟨hal-01878957⟩
  • Manjeera Gowravaram, Fabien Bonneau, Joanne Kanaan, Vincent Maciej, Francesca Fiorini, et al.. A conserved structural element in the RNA helicase UPF1 regulates its catalytic activity in an isoform-specific manner. Nucleic Acids Research, Oxford University Press, 2018, 46 (5), pp.2648 - 2659. ⟨10.1093/nar/gky040⟩. ⟨hal-01762546⟩
  • Francesca Fiorini, Jean-Philippe Robin, Joanne Kanaan, Malgorzata Borowiak, Vincent Croquette, et al.. HTLV-1 Tax plugs and freezes UPF1 helicase leading to nonsense-mediated mRNA decay inhibition. Nature Communications, Nature Publishing Group, 2018, 9 (1), ⟨10.1038/s41467-017-02793-6⟩. ⟨hal-02358887⟩
  • Samar Hodeib, Saurabh Raj, Maria Manosas, Weiting Zhang, Debjani Bagchi, et al.. A mechanistic study of helicases with magnetic traps. Protein Science, Wiley, 2017, 26 (7), pp.1314-1336. ⟨10.1002/pro.3187⟩. ⟨hal-02358906⟩
  • Francesca Fiorini, Debjani Bagchi, Hervé Le Hir, Vincent Croquette. Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities. Nature Communications, Nature Publishing Group, 2015, 6 (1), ⟨10.1038/ncomms8581⟩. ⟨hal-02358918⟩
  • Francesca Fiorini, Marc Boudvillain, Hervé Le Hir. Tight intramolecular regulation of the human Upf1 helicase by its N- and C-terminal domains. Nucleic Acids Research, Oxford University Press, 2013, 41, pp.2404-2415. ⟨10.1093/nar/gks1320⟩. ⟨hal-00817448⟩
  • Francesca Fiorini, Marc Boudvillain, Hervé Le Hir. Tight intramolecular regulation of the human Upf1 helicase by its N- and C-terminal domains. Nucleic Acids Research, Oxford University Press, 2013, 41 (4), pp.2404-2415. ⟨10.1093/nar/gks1320⟩. ⟨hal-02358926⟩
  • Elisabeth Daguenet, Aurélie Baguet, Sébastien Degot, Ute Schmidt, Fabien Alpy, et al.. Perispeckles are major assembly sites for the exon junction core complex.. Molecular Biology of the Cell, American Society for Cell Biology, 2012, 23 (9), pp.1765-1782. ⟨10.1091/mbc.E12-01-0040⟩. ⟨hal-00727654⟩
  • Isabelle Barbosa, Nazmul Haque, Francesca Fiorini, Charlotte Barrandon, Catherine Tomasetto, et al.. Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly. Nature Structural and Molecular Biology, Nature Publishing Group, 2012, 19 (10), pp.983-990. ⟨10.1038/nsmb.2380⟩. ⟨hal-02358933⟩
  • Jérôme Saulière, Valentine Murigneux, Zhen Wang, Emélie Marquenet, Isabelle Barbosa, et al.. CLIP-seq of eIF4AIII reveals transcriptome-wide mapping of the human exon junction complex.. Nature Structural and Molecular Biology, Nature Publishing Group, 2012, 19 (11), pp.1124-31. ⟨10.1038/nsmb.2420⟩. ⟨hal-00982626⟩
  • Sutapa Chakrabarti, Uma Jayachandran, Fabien Bonneau, Francesca Fiorini, Claire Basquin, et al.. Molecular Mechanisms for the RNA-Dependent ATPase Activity of Upf1 and Its Regulation by Upf2. Molecular Cell, Elsevier, 2011, 41 (6), pp.693-703. ⟨10.1016/j.molcel.2011.02.010⟩. ⟨hal-02358974⟩
  • Aurélie Baguet, Sébastien Degot, Nicolas Cougot, Edouard Bertrand, Marie-Pierre Chenard, et al.. The exon-junction-complex-component metastatic lymph node 51 functions in stress-granule assembly.. Journal of Cell Science, Company of Biologists, 2007, 120 (Pt 16), pp.2774-84. ⟨10.1242/jcs.009225⟩. ⟨hal-00189164⟩
  • Karine Buchet-Poyau, Julien Courchet, Hervé Le Hir, Bertrand Séraphin, Jean-Yves Scoazec, et al.. Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies.. Nucleic Acids Research, Oxford University Press, 2007, 35 (4), pp.1289-300. ⟨10.1093/nar/gkm016⟩. ⟨hal-00167447⟩
  • Christian B F Andersen, Lionel Ballut, Jesper S Johansen, Hala Chamieh, Klaus H Nielsen, et al.. Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.. Science, American Association for the Advancement of Science, 2006, 313 (5795), pp.1968-72. ⟨10.1126/science.1131981⟩. ⟨hal-00168014⟩
  • Lionel Ballut, Brice Marchadier, Aurélie Baguet, Catherine Tomasetto, Bertrand Séraphin, et al.. The exon junction core complex is locked onto RNA by inhibition of eIF4AIII ATPase activity.. Nature Structural and Molecular Biology, Nature Publishing Group, 2005, 12 (10), pp.861-9. ⟨10.1038/nsmb990⟩. ⟨hal-00187421⟩

Book sections1 document

Preprints, Working Papers, ...2 documents

  • Racha Chouaib, Adham Safieddine, Xavier Pichon, Arthur Imbert, Oh Sung Kwon, et al.. A localization screen reveals translation factories and widespread co-translational RNA targeting. 2020. ⟨pasteur-02873144⟩
  • Oh Sung Kwon, Rahul Mishra, Adham Safieddine, Emeline Coleno, Quentin Alasseur, et al.. Exon Junction Complex dependent mRNA localization is linked to centrosome organization during ciliogenesis. 2020. ⟨hal-03033097⟩