Giuliano Sciara

**Address** <a name="_Hlt476690674"></a>Laboratory “Biodiversity and Biotechnology of Fungi” BBF UMR1163 Aix-Marseille Université / INRAE - Polytech Marseille, Faculté des Sciences de Luminy - 163, Av de Luminy - 13009 Marseille, France tel: +33 6 2385 6888 / email: giuliano.sciara@inrae.fr **Personal Information** Date of birth: 10/03/1973 ========================= Place of birth: Rimini (ITALY) Nationality: Italian **Work Experience** Since 01/01/2017: **INRAE Researcher** (Chargé de Recherche). Unit: BBF - UMR1163 Aix-Marseille Université / INRAE. 01/02/2015 - 31/12/2016: **PostDoc** BBF UMR1163 Aix-Marseille Université / INRAE **-** Marseille, FRANCE (Dr. J-G. Berrin) (Characterization of Fungal Enzymes involved in Plant Cell Wall degradation). 01/04/2013 - 31/03/2014: **PostDoc (Marie Curie IOF fellow)** BIP UMR7281 Aix-Marseille Université / CNRS **-** Marseille, FRANCE (Dr. M.T. Giudici-Orticoni) (Structural Studies of Respiratory Chain Metalloproteins from Extremophiles). 01/01/2010 - 31/03/2013: **PostDoc (2011 - 13: Marie Curie IOF fellow)** Dept. of Physiology and Cellular Biophysics - Columbia University - New York, USA (Prof. F. Mancia) (Membrane Proteins: Production, Stability by high-throughput approaches and Crystallography). 01/01/2005 - 31/12/2009: **PostDoc (2006 - 07: Marie Curie EIF fellow)** AFMB UMR6098 - Aix-Marseille Université / CNRS **-** Marseille, FRANCE (Dr. C. Cambillau) (Membrane Proteins and Large Macromoleculaar Complexes: high-throughput approaches, Solution Studies by Light Scattering and Structural Studies by Crystallography). 10/02 - 30/09/2000; 01/10/2003 - 31/12/2004: **Predoctoral Internship / PostDoc** Dipart. Sc. Biochimiche - Università « La Sapienza » **-** Roma, ITALY (Prof. M. Brunori and B. Vallone) (Structural Studies of Enzymes and Globins, , Structural Investigation of Protein Conformational Changes, Mechanisms of Natural Antibiotics Biosynthesis). **Scientific Skills** \- Studied Systems: plant cell wall active Enzymes, Metalloproteins, large Macromolecular Assemblies and Membrane Proteins. \- Structural Studies: crystallization and crystallography (data collection and analysis); building and analysis of protein models; NMR. \- Solution Studies: light scattering (aggregation state, protein-protein and protein-detergent interactions and stoichiometry, protein glycosylation); analytical HPLC; spectroscopies (UV/Vis, Fluorescence, CD). \- Molecular Biology and Protein Production in *E. coli*, *A. niger* and *P. pastoris*: cloning; protein engineering; protein purification; high-throughput approaches. **Other Professional Skills** \- Scientific Writing: applications for funding and fellowships, articles, reports. \- Scientific Presentations: conferences, meetings. \- Informatics: Office, graphics packages, scientific software. Operating systems: MacOS, Linux, PC. \- Group Management and Tutoring (students and technicians). \- Project Management: strategies, problem solving, protocol design, execution. \- Lab Management: section responsibilities, platform setup and supervision. \- Languages: Italian (mother tongue); English (proficient user); French (proficient user). **Teaching and Tutoring Activities** \- Since 2005: (co-)tutoring of technicians, bachelor, master and PhD students. \- 2015-16: fixed-term lecturer for master (BBSG, 1st and 2nd year) and bachelor courses (Aix-Marseille Université): lectures on protein crystallography. \- 2003-2004: teaching activities for the Chemistry and Introduction to Biochemistry Course (Medicine Faculty “La Sapienza”): tutorials and laboratory classes (titrations, buffer systems, protein precipitation at their pI); member of examination committees; ADE “Elective Didactic Activity” (lecture on radioactivity and its applications in medicine). **Fellowships and Distinctions** 2011: Postdoctoral Fellowship (3 years) : Marie Curie International Outgoing Fellowship (IOF) 2006: Postdoctoral Fellowship (2 years) : Marie Curie Intra European Fellowship (EIF) 2004: PhD Thesis Prize « Mario Rippa - 2003/2004 » 1995: ERASMUS Fellowship (3 months) : Laboratory for Crystallography, Universiteit van Amsterdam – NETHERLANDS (Prof. H. Schenk) **Education and Qualifications** 2015: **Qualification Maitre de Conférence** (equivalent to “Abilitazione Prof. Associato”) - Ministère de L'Education Française - Section 64: « Biochemistry and Molecular Biology ». 2004: **Esame di Stato** (Biologo) - Ministero Italiano dell’Istruzione. 2004: **PhD in Biochemistry** - Dipart. Sc. Biochimiche - Università « La Sapienza » **-** Roma, ITALY (Prof. M. Brunori and B. Vallone) PhD Thesis: « The crystal structure at 1.3 Å resolution of ActVA-orf6 from *Streptomyces coelicolor*, a monooxygenase without cofactors ». 1999: **Master Degree in Biology** (Laurea cum laude) Dipart. Chimica Agraria - Università di Bologna **-** Bologna, ITALY (Prof. S. Ciurli) Master Thesis: « Solution structure of oxidized cytochrome *c*553 from *B. pasteurii* through NMR spectroscopy ». 1987 - 92: **Italian high school:** Liceo Scientifico “A. Volta”, Riccione – ITALY. 1987 - 92: **Music high school:** Conservatorio “G. Rossini”, Pesaro – ITALY.