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Giuliano SCIARA



Laboratory “Biodiversity and Biotechnology of Fungi” BBF UMR1163

Aix-Marseille Université / INRAE - Polytech Marseille, Faculté des Sciences de Luminy - 163, Av de Luminy - 13009 Marseille, France

tel: +33 6 2385 6888   /   email:

Personal Information

Date of birth: 10/03/1973

Place of birth: Rimini (ITALY)

Nationality: Italian


Work Experience

Since 01/01/2017: INRAE Researcher (Chargé de Recherche). Unit: BBF - UMR1163 Aix-Marseille Université / INRAE.

01/02/2015 - 31/12/2016:  PostDoc

BBF UMR1163 Aix-Marseille Université / INRAE - Marseille, FRANCE   (Dr. J-G. Berrin)

(Characterization of Fungal Enzymes involved in Plant Cell Wall degradation).

01/04/2013 - 31/03/2014:  PostDoc (Marie Curie IOF fellow)

BIP UMR7281 Aix-Marseille Université / CNRS - Marseille, FRANCE   (Dr. M.T. Giudici-Orticoni)

(Structural Studies of Respiratory Chain Metalloproteins from Extremophiles).

01/01/2010 - 31/03/2013:  PostDoc (2011 - 13: Marie Curie IOF fellow)

Dept. of Physiology and Cellular Biophysics - Columbia University - New York, USA   (Prof. F. Mancia)

(Membrane Proteins: Production, Stability by high-throughput approaches and Crystallography).

01/01/2005 - 31/12/2009:  PostDoc (2006 - 07: Marie Curie EIF fellow)

AFMB UMR6098 - Aix-Marseille Université / CNRS - Marseille, FRANCE   (Dr. C. Cambillau)

(Membrane Proteins and Large Macromoleculaar Complexes: high-throughput approaches, Solution Studies by Light Scattering and Structural Studies by Crystallography).

10/02 - 30/09/2000; 01/10/2003 - 31/12/2004:           Predoctoral Internship / PostDoc

Dipart. Sc. Biochimiche - Università « La Sapienza » - Roma, ITALY   (Prof. M. Brunori and B. Vallone)

(Structural Studies of Enzymes and Globins, , Structural Investigation of Protein Conformational Changes, Mechanisms of Natural Antibiotics Biosynthesis).


Scientific Skills

-     Studied Systems: plant cell wall active Enzymes, Metalloproteins, large Macromolecular Assemblies and Membrane Proteins.

-     Structural Studies: crystallization and crystallography (data collection and analysis); building and analysis of protein models; NMR.

-     Solution Studies: light scattering (aggregation state, protein-protein and protein-detergent interactions and stoichiometry, protein glycosylation); analytical HPLC; spectroscopies (UV/Vis, Fluorescence, CD).

-     Molecular Biology and Protein Production in E. coli, A. niger and P. pastoris: cloning; protein engineering; protein purification; high-throughput approaches.


Other Professional Skills

-     Scientific Writing: applications for funding and fellowships, articles, reports.

-     Scientific Presentations: conferences, meetings.

-     Informatics: Office, graphics packages, scientific software. Operating systems: MacOS, Linux, PC.

-     Group Management and Tutoring (students and technicians).

-     Project Management: strategies, problem solving, protocol design, execution.

-     Lab Management: section responsibilities, platform setup and supervision.

-     Languages: Italian (mother tongue); English (proficient user); French (proficient user).


Teaching and Tutoring Activities

-     Since 2005: (co-)tutoring of technicians, bachelor, master and PhD students.

-     2015-16: fixed-term lecturer for master (BBSG, 1st and 2nd year) and bachelor courses (Aix-Marseille Université): lectures on protein crystallography.

-     2003-2004: teaching activities for the Chemistry and Introduction to Biochemistry Course (Medicine Faculty “La Sapienza”): tutorials and laboratory classes (titrations, buffer systems, protein precipitation at their pI); member of examination committees; ADE “Elective Didactic Activity” (lecture on radioactivity and its applications in medicine).


Fellowships and Distinctions

2011:         Postdoctoral Fellowship (3 years) : Marie Curie International Outgoing Fellowship (IOF)

2006:         Postdoctoral Fellowship (2 years) : Marie Curie Intra European Fellowship (EIF)


2004:         PhD Thesis Prize « Mario Rippa - 2003/2004 »

1995:         ERASMUS Fellowship (3 months) : Laboratory for Crystallography, Universiteit van Amsterdam – NETHERLANDS   (Prof. H. Schenk)


Education and Qualifications

2015:   Qualification Maitre de Conférence (equivalent to “Abilitazione Prof. Associato”) - Ministère de L'Education Française - Section 64: « Biochemistry and Molecular Biology ».

2004:   Esame di Stato (Biologo) - Ministero Italiano dell’Istruzione.

2004:   PhD in Biochemistry - Dipart. Sc. Biochimiche - Università « La Sapienza » - Roma, ITALY   (Prof. M. Brunori and B. Vallone)

PhD Thesis: « The crystal structure at 1.3 Å resolution of ActVA-orf6 from Streptomyces coelicolor, a monooxygenase without cofactors ».


1999:   Master Degree in Biology (Laurea cum laude)

Dipart. Chimica Agraria - Università di Bologna - Bologna, ITALY   (Prof. S. Ciurli)

Master Thesis: « Solution structure of oxidized cytochrome c553 from B. pasteurii through NMR spectroscopy ».

1987 - 92: Italian high school: Liceo Scientifico “A. Volta”, Riccione – ITALY.

1987 - 92:  Music high school: Conservatorio “G. Rossini”, Pesaro – ITALY.


Journal articles29 documents

  • Sona Garajova, Ilabahen Patel, Anne Lomascolo, Frédéric F. Legée, Laurent L. Cezard, et al.. Treatment of wood fbres with laccases: improved hardboard properties through phenolic oligomerization. European Journal of Wood and Wood Products, Springer Verlag, 2021. ⟨hal-03512563⟩
  • Amal Ben Ayed, Geoffroy Saint-Genis, Laurent Vallon, Dolores Linde, Annick Turbé-Doan, et al.. Exploring the Diversity of Fungal DyPs in Mangrove Soils to Produce and Characterize Novel Biocatalysts. Journal of Fungi, 2021, 7 (5), pp.321. ⟨10.3390/jof7050321⟩. ⟨hal-03215284⟩
  • Corinne Ivaldi, Mariane Daou, Laurent Vallon, Alexandra Bisotto, Mireille Haon, et al.. Screening New Xylanase Biocatalysts from the Mangrove Soil Diversity. Microorganisms, MDPI, 2021, 9 (7), pp.1-16. ⟨10.3390/microorganisms9071484⟩. ⟨insu-03293424⟩
  • Gabriele Cerutti, Elena Gugole, Linda Celeste Montemiglio, Annick Turbé-Doan, Dehbia Chena, et al.. Crystal structure and functional characterization of an oligosaccharide dehydrogenase from Pycnoporus cinnabarinus provides insights into fungal breakdown of lignocellulose. Biotechnology for Biofuels, 2021, 14, pp.161. ⟨10.1186/s13068-021-02003-y⟩. ⟨hal-03344334⟩
  • Wissal Ben Ali, Amal Ben Ayed, Annick Turbé-Doan, Emmanuel Bertrand, Yann Mathieu, et al.. Enzyme Properties of a Laccase Obtained from the Transcriptome of the Marine-Derived Fungus Stemphylium lucomagnoense. International Journal of Molecular Sciences, MDPI, 2020, 21 (21), pp.8402. ⟨10.3390/ijms21218402⟩. ⟨hal-03000290⟩
  • Wissal Ben Ali, David Navarro, Abhishek Kumar, Elodie Drula, Annick Turbé-Doan, et al.. Characterization of the CAZy Repertoire from the Marine-Derived Fungus Stemphylium lucomagnoense in Relation to Saline Conditions. Marine drugs, MDPI, 2020, 18 (9), pp.1-21. ⟨10.3390/md18090461⟩. ⟨hal-02953538⟩
  • Wissal Ben Ali, Delphine Chaduli, David Navarro, Christian Lechat, Annick Turbé-Doan, et al.. Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications. BMC Biotechnology, 2020, 20 (1), ⟨10.1186/s12896-020-00617-y⟩. ⟨hal-02928294⟩
  • Saowanee Wikee, Juliette Hatton, Annick Turbé-Doan, Yann Mathieu, Marianne Daou, et al.. Characterization and Dye Decolorization Potential of Two Laccases from the Marine-Derived Fungus Pestalotiopsis sp.. International Journal of Molecular Sciences, MDPI, 2019, 20 (8), pp.1864. ⟨10.3390/ijms20081864⟩. ⟨hal-02276821⟩
  • Magali Roger, Giuliano Sciara, Frédéric Biaso, Elisabeth Lojou, Xie Wang, et al.. Impact of copper ligand mutations on a cupredoxin with a green copper center.. BBA - Biochimica et Biophysica Acta, Elsevier, 2017, 1858 (5), pp.351-359. ⟨10.1016/j.bbabio.2017.02.007⟩. ⟨hal-01481398⟩
  • Linda Celeste Montemiglio, Giacomo Parisi, Antonella Scaglione, Giuliano Sciara, Carmelinda Savino, et al.. Functional analysis and crystallographic structure of clotrimazole bound OleP, a cytochrome P450 epoxidase from Streptomyces antibioticus involved in oleandomycin biosynthesis.. Biochimica et Biophysica Acta (BBA) - General Subjects, 2016, 1860 (3), pp.465-475. ⟨10.1016/j.bbagen.2015.10.009⟩. ⟨hal-01581613⟩
  • Magali Roger, Frédéric Biaso, Cindy J. Castelle, Marielle Bauzan, Florence Chaspoul, et al.. Spectroscopic Characterization of a Green Copper Site in a Single-Domain Cupredoxin. PLoS ONE, Public Library of Science, 2014, 9 (6), pp.e98941. ⟨10.1371/journal.pone.0098941⟩. ⟨hal-01494439⟩
  • Giuliano Sciara, Oliver B Clarke, David Tomasek, Brian Kloss, Shantelle Tabuso, et al.. Structural basis for catalysis in a CDP-alcohol phosphotransferase.. Nature Communications, Nature Publishing Group, 2014, 5, pp.4068. ⟨10.1038/ncomms5068⟩. ⟨hal-01594683⟩
  • Giuliano Sciara, Filippo Mancia. Highlights from recently determined structures of membrane proteins: a focus on channels and transporters.. Current Opinion in Structural Biology, Elsevier, 2012, 22 (4), pp.476-81. ⟨10.1016/⟩. ⟨hal-01615073⟩
  • Giuliano Sciara, Cecilia Bebeacua, Patrick Bron, Denise Tremblay, Miguel Ortiz-Lombardia, et al.. Structure of lactococcal phage p2 baseplate and its mechanism of activation.. Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2010, 107 (15), pp.6852-7. ⟨10.1073/pnas.1000232107⟩. ⟨hal-01595268⟩
  • Kerstin Michalke, Céline Huyghe, Julie Lichière, Marie-Eve Gravière, Marina Siponen, et al.. Mammalian G protein-coupled receptor expression in Escherichia coli: II. Refolding and biophysical characterization of mouse cannabinoid receptor 1 and human parathyroid hormone receptor 1.. Analytical Biochemistry, Elsevier Masson, 2010, 401 (1), pp.74-80. ⟨10.1016/j.ab.2010.02.017⟩. ⟨hal-01595269⟩
  • Valérie Campanacci, David Veesler, Julie Lichière, Stéphanie Blangy, Giuliano Sciara, et al.. Solution and electron microscopy characterization of lactococcal phage baseplates expressed in Escherichia coli.. Journal of Structural Biology, Elsevier, 2010, 172 (1), pp.75-84. ⟨10.1016/j.jsb.2010.02.007⟩. ⟨hal-01595270⟩
  • Carmelinda Savino, Linda C Montemiglio, Giuliano Sciara, Adriana E Miele, Steven G Kendrew, et al.. Investigating the structural plasticity of a cytochrome P450: three-dimensional structures of P450 EryK and binding to its physiological substrate.. Journal of Biological Chemistry, 2009, 284 (42), pp.29170-9. ⟨10.1074/jbc.M109.003590⟩. ⟨hal-01617673⟩
  • Carmelinda Savino, Adriana E Miele, Federica Draghi, Kenneth A Johnson, Giuliano Sciara, et al.. Pattern of cavities in globins: the case of human hemoglobin.. Biopolymers, 2009, 91 (12), pp.1097-1107. ⟨10.1002/bip.21201⟩. ⟨hal-01617675⟩
  • Marina Siponen, Giuliano Sciara, Manuela Villion, Silvia Spinelli, Julie Lichière, et al.. Crystal structure of ORF12 from Lactococcus lactis phage p2 identifies a tape measure protein chaperone.. Journal of Bacteriology, American Society for Microbiology, 2009, 191 (3), pp.728-34. ⟨10.1128/JB.01363-08⟩. ⟨hal-01615930⟩
  • Marion Giffard, Vanessa Delfosse, Giuliano Sciara, Claudine Mayer, Christian Cambillau, et al.. Surfactant Poloxamer 188 as a New Crystallizing Agent for Urate Oxidase. Crystal Growth & Design, American Chemical Society, 2009, 9 (9), pp.4199-4206. ⟨10.1021/cg900473n⟩. ⟨hal-01617674⟩
  • David Veesler, Stéphanie Blangy, Marina Siponen, Renaud Vincentelli, Christian Cambillau, et al.. Production and biophysical characterization of the CorA transporter from Methanosarcina mazei.. Analytical Biochemistry, Elsevier Masson, 2009, 388 (1), pp.115-21. ⟨10.1016/j.ab.2009.02.018⟩. ⟨hal-01615932⟩
  • Carmelinda Savino, Giuliano Sciara, Adriana E Miele, Steven G Kendrew, Beatrice Vallone. Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of C-12 hydroxylase EryK from Saccharopolyspora erythraea.. Protein and Peptide Letters, 2008, 15 (10), pp.1138-1141. ⟨10.2174/092986608786071201⟩. ⟨hal-01617667⟩
  • David Veesler, Stéphanie Blangy, Christian Cambillau, Giuliano Sciara. There is a baby in the bath water: AcrB contamination is a major problem in membrane-protein crystallization.. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, International Union of Crystallography, 2008, 64 (Pt 10), pp.880-885. ⟨10.1107/S1744309108028248⟩. ⟨hal-01617665⟩
  • Giuliano Sciara, Stéphanie Blangy, Marina I Siponen, Stephen Mc Grath, Douwe van Sinderen, et al.. A Topological Model of the Baseplate of Lactococcal Phage Tuc2009. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2008, 283 (5), pp.2716-2723. ⟨10.1074/jbc.M707533200⟩. ⟨hal-02066265⟩
  • Dominique Bourgeois, Beatrice Vallone, Alessandro Arcovito, Giuliano Sciara, Friedrich Schotte, et al.. Extended subnanosecond structural dynamics of myoglobin revealed by Laue crystallography.. Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2006, 103 (13), pp.4924-4929. ⟨10.1073/pnas.0508880103⟩. ⟨hal-01617679⟩
  • Adriana E Miele, Luca Federici, Giuliano Sciara, Federica Draghi, Maurizio Brunori, et al.. Analysis of the effect of microgravity on protein crystal quality: the case of a myoglobin triple mutant.. Acta crystallographica Section D : Structural biology [1993-..], 2003, 59 (6), pp.982-928. ⟨hal-01595187⟩
  • Dominique Bourgeois, Beatrice Vallone, Friedrich Schotte, Alessandro Arcovito, Adriana E Miele, et al.. Complex landscape of protein structural dynamics unveiled by nanosecond Laue crystallography.. Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2003, 100 (15), pp.8704-9. ⟨10.1073/pnas.1430900100⟩. ⟨hal-01595186⟩
  • Giuliano Sciara, Steven G Kendrew, Adriana E Miele, Neil G Marsh, Luca Federici, et al.. The structure of ActVA-Orf6, a novel type of monooxygenase involved in actinorhodin biosynthesis.. EMBO Journal, 2003, 22 (2), pp.205-15. ⟨10.1093/emboj/cdg031⟩. ⟨hal-01595326⟩
  • Lucia Banci, Ivano Bertini, Stefano Ciurli, Alexander Dikiy, Jens Dittmer, et al.. NMR Solution Structure, Backbone Mobility, and Homology Modeling ofc-Type Cytochromes from Gram-Positive Bacteria. ChemBioChem, 2002, 3 (4), pp.299-310. ⟨10.1002/1439-7633(20020402)3:4<299::AID-CBIC299>3.0.CO;2-0⟩. ⟨hal-03259473⟩

Conference papers1 document

  • L Barchi, Acquadro A, E. Portis, Alonso D, P. Gramazio, et al.. Capsicum spp. and eggplant genome sequencing and resequencing provide new tools for the characterization of genetic resources. 17th Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant, Sep 2019, Avignon, France. ⟨hal-03551755⟩

Conference poster1 document

  • Eric Record, Emmanuel Bertrand, Annick Doan, Laura Munoz-Duarte, Théo Dupont, et al.. Fungal Oxido-reductase models enzymology structural data and synergy for biotechnological applications. Oxizymes, Jul 2018, Belfast, Ireland. ⟨hal-01853256⟩

Preprints, Working Papers, ...2 documents

  • Magali Roger, Philippe Leone, Ninan Blackburn, Sam Horrell, Tadeo Moreno Chicano, et al.. Three-dimensional structure of the single domain cupredoxin AcoP. {date}. ⟨hal-03866634⟩
  • Gabriele Cerutti, Elena Gugole, Linda Celeste Montemiglio, Annick Turbé-Doan, Dehbia Chena, et al.. Identification of an Oligosaccharide Dehydrogenase from Pycnoporus Cinnabarinus Provides Insights into Fungal Breakdown of Lignocellulose. {date}. ⟨hal-03259451⟩