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Frédéric Cassé

Research Engineer in biology
33
Documents
Identifiants chercheurs

Présentation

### **CURRENT POSITION** ###### **Research engineer in Biology**, University of Caen, UNICAEN, France. > > Plateform Normandie Equine Vallée. > Team: UR 7450 BIOTARGEN Biology, genetics and osteoarticular and respiratory therapies. ### **ACADEMIC QUALIFICATIONS** ###### **Ph.D**. **in Molecular and Cellular Biology**, Sept 2008 - Oct 2011 > University of Caen, France awarded October 12th, 2011. > ###### **M.Sc. Molecular and cellular Biology,** Sept 2006 - June 2008 > Life sciences and health, specialty cell biology, University of Caen, France. > ###### **Bachelor's degree**, Sept 2005 - June 2006 > Life sciences, Science and technology, University of Caen, France. ### **RESEARCH EXPERIENCE** ###### **Research engineer in Biology**, Dec 2019 - present Plateform Normandie Equine Vallée. University of Caen, UNICAEN, France. Team: UR 7450 BIOTARGEN Biology, genetics and osteoarticular and respiratory therapies. Director: Dr Magali Demoor, co-director : Dr Nicolas Gruchy. Project: *Cellular therapy and tissue engineering for articular cartilage repair.* ###### **Research scientist**, Apr 2017 - Nov 2019 > Center for psychiatric neurosciences. Lausanne University Hospital, Switzerland. > Team: Adult hippocampal neurogenesis: from regulation mechanisms to functional impact. PI: Pr Nicolas Toni. > Project: *Roles of astrocytes in adult hippocampal neurogenesis and mood disorder.* ###### **Post-Doctoral position**, Mar 2015 - Mar 2017 Department of Fundamental Neurosciences. University of Lausanne, Switzerland. Team: Synaptic integration of new neurons in the adult hippocampus. PI: Pr Nicolas Toni. Project: *Control of adult hippocampal neurogenesis by astrocytes.* ###### **Post-Doctoral position,** Mar 2012 - Feb 2015 Institute of Molecular and Cellular Pharmacology. University of Nice, France. Team: Sumoylation in neuronal function and dysfunction. PI: Dr Stéphane Martin. Project: *Regulation of the sumoylation machinery in neuronal and glial cells.* ###### **Ph.D**. **training,** in Molecular and Cellular Biology, Sept 2008 - Oct 2011 University of Caen, France. Team: Serine Proteases and Pathophysiology of the neurovascular Unit. Supervisors: Pr Denis Vivien and Dr Fabian Docagne. Thesis title: Tissue type plasminogen activator: a new gliotransmitter of tripartite glutamatergic synapse. Defended October 12th, 2011. ### **TECHNICAL SKILLS** **Cellular Biology** Isolation and culture of equine and human articular chondrocytes, mesenchymal stem cell derived from bone marrow and umbilical cord blood (Equine). 3D cartilage organoid models. Isolation and culture of adult neuronal stem cell (rodent), primary cultures of rodent’s hippocampal and cortical neurons, astrocytes and mixed cultures of neurons and astrocytes, adherent mammalian cell lines (COS-7, HEK293T, CHO), fibroblast cell culture. Transfection and preparation of viruses (sindbis virus, lentivirus, moloney murine leukemia virus). Models of cell death. Test of cytotoxicity and apoptosis, proliferation and wound-healing assay. **Microbiology** Isolation and culture, bacterial transformation, plasmid amplification, gram coloration, API identification. **Molecular Biology** RNA and DNA extraction from cell culture and tissue, real-time quantitative PCR, transient transfection, siRNA transfection, plasmid construction and subcloning, site-specific mutagenesis, genotyping. **Biochemistry** SDS-Page, western blotting, dot-blot, immunoprecipitation, zymography assay, sumoylation assay, synaptosomal preparation, protein purification techniques. **Live cell Imaging** Fluorescence recovery after photobleaching (FRAP)/Fluorescence loss in photobleaching (FLIP) experiments, Dendra photoconversion, cell surface protein mobility trafficking with Super Ecliptic Phluorin, protein mobility trafficking with photoswitchable fluorescent protein Dendra2, calcium imaging, mitochondria imaging (Mito Tracker, Mito Timer), cell proliferation assay. **Microscopy** Immunohistochemistry, immunocytochemistry, acquisition and analyses of confocal images. **Rodent Surgery** Stereotactic surgery, intraperitoneal, intravenous, and intracerebral injections. **Behavioral tests** Morris water maze, object recognition and location, fear conditioning, place recognition. **Technical Software** Image J (Analysis and macro programming),Imaris,Volocity, zen, NIS, Snapgene, Scalfold proteome software, Funrich, GraphPad Prism,JMP, Statview, Photoshop, Canvas, Microsoft office.

Publications

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Effect of pro-inflammatory cytokine priming and storage temperature of the mesenchymal stromal cell (MSC) secretome on equine articular chondrocytes

Manon Jammes , Romain Contentin , Fabrice Audigié , Frédéric Cassé , Philippe Galéra
Frontiers in Bioengineering and Biotechnology, 2023, 11, pp.1204737. ⟨10.3389/fbioe.2023.1204737⟩
Article dans une revue hal-04197029v1
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Pro-Inflammatory Cytokine Priming and Purification Method Modulate the Impact of Exosomes Derived from Equine Bone Marrow Mesenchymal Stromal Cells on Equine Articular Chondrocytes

Manon Jammes , Frédéric Cassé , Emilie Velot , Arnaud Bianchi , Fabrice Audigié
International Journal of Molecular Sciences, 2023, 24 (18), pp.14169. ⟨10.3390/ijms241814169⟩
Article dans une revue hal-04221506v1
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Equine osteoarthritis: Strategies to enhance mesenchymal stromal cell-based acellular therapies

Manon Jammes , Romain Contentin , Frédéric Cassé , Philippe Galéra
Frontiers in Veterinary Science, 2023, 10 (1115774), ⟨10.3389/fvets.2023.1115774⟩
Article dans une revue hal-04042900v1
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Functionalized Nanogels with Endothelin-1 and Bradykinin Receptor Antagonist Peptides Decrease Inflammatory and Cartilage Degradation Markers of Osteoarthritis in a Horse Organoid Model of Cartilage

Aurélie Cullier , Frédéric Cassé , Seng Manivong , Romain Contentin , Florence Legendre
International Journal of Molecular Sciences, 2022, 23 (16), pp.8949. ⟨10.3390/ijms23168949⟩
Article dans une revue hal-03775355v1

Synaptic Adhesion Molecules Regulate the Integration of New Granule Neurons in the Postnatal Mouse Hippocampus and their Impact on Spatial Memory

Marine Krzisch , Christine Fülling , Laura Jabinet , Jan Armida , Elias Gebara
Cerebral Cortex, 2022, ⟨10.1093/cercor/bhw217⟩
Article dans une revue hal-03896703v1
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Bone Marrow MSC Secretome Increases Equine Articular Chondrocyte Collagen Accumulation and Their Migratory Capacities

Romain Contentin , Manon Jammes , Bastien Bourdon , Frédéric Cassé , Arnaud Bianchi
International Journal of Molecular Sciences, 2022, 23 (10), pp.5795. ⟨10.3390/ijms23105795⟩
Article dans une revue hal-03695375v1
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Marine Collagen Hydrolysates Downregulate the Synthesis of Pro-Catabolic and Pro-Inflammatory Markers of Osteoarthritis and Favor Collagen Production and Metabolic Activity in Equine Articular Chondrocyte Organoids

Bastien Bourdon , Romain Contentin , Frédéric Cassé , Chloé Maspimby , Sarah Oddoux
International Journal of Molecular Sciences, 2021, 22 (2), pp.580. ⟨10.3390/ijms22020580⟩
Article dans une revue hal-03114578v1
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Marine Collagen Hydrolysates Promote Collagen Synthesis, Viability and Proliferation While Downregulating the Synthesis of Pro-Catabolic Markers in Human Articular Chondrocytes

Bastien Bourdon , Frédéric Cassé , Nicolas Gruchy , Pierre Cambier , Sylvain Leclercq
International Journal of Molecular Sciences, 2021, 22 (7), pp.3693-3718. ⟨10.3390/ijms22073693⟩
Article dans une revue hal-03215150v1
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The synaptic balance between sumoylation and desumoylation is maintained by the activation of metabotropic mGlu5 receptors

Lenka Schorova , Marie Pronot , Gwenola Poupon , Marta Prieto , Alessandra Folci
Cellular and Molecular Life Sciences, 2019, 76 (15), pp.3019-3031. ⟨10.1007/s00018-019-03075-8⟩
Article dans une revue inserm-02474777v1
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Sumoylation regulates FMRP-mediated dendritic spine elimination and maturation

Anouar Khayachi , Carole Gwizdek , Gwénola Poupon , Damien Alcor , Magda Chafai
Nature Communications, 2018, 9 (1), pp.757. ⟨10.1038/s41467-018-03222-y⟩
Article dans une revue inserm-02443557v1

Astrocytes’ Contribution to Adult Neurogenesis in Physiology and Alzheimer’s Disease

Frédéric Cassé , Kevin Richetin , Nicolas Toni
Frontiers in Cellular Neuroscience, 2018, 12, pp.432. ⟨10.3389/fncel.2018.00432⟩
Article dans une revue hal-03789897v1

Sumoylation regulates FMRP-mediated dendritic spine elimination and maturation

Anouar Khayachi , Carole Gwizdek , Gwénola Poupon , Damien Alcor , Magda Chafai
Nature Communications, 2018, 9 (1), ⟨10.1038/s41467-018-03222-y⟩
Article dans une revue hal-03084675v1

Mossy Cells Control Adult Neural Stem Cell Quiescence and Maintenance through a Dynamic Balance between Direct and Indirect Pathways

Chia-Yu Yeh , Brent Asrican , Jonathan Moss , Luis Jhoan Quintanilla , Ting He
Neuron, 2018, 99 (3), pp.493-510.e4. ⟨10.1016/j.neuron.2018.07.010⟩
Article dans une revue hal-03896715v1

Sumoylation regulates FMRP-mediated dendritic spine elimination and maturation

Anouar Khayachi , Carole Gwizdek , Gwénola Poupon , Damien Alcor , Magda Chafai
Nature Communications, 2018, 9 (1), ⟨10.1038/s41467-018-03222-y⟩
Article dans une revue hal-03039173v1

Interactions between N -Ethylmaleimide-sensitive factor and GluA2 contribute to effects of glucocorticoid hormones on AMPA receptor function in the rodent hippocampus

Hui Xiong , Frédéric Cassé , Ming Zhou , Zhi-Qi Xiong , Marian Joels
Hippocampus, 2016, 26 (7), pp.848-856. ⟨10.1002/hipo.22567⟩
Article dans une revue inserm-03494824v1

Synaptic Integration of Adult-Born Hippocampal Neurons Is Locally Controlled by Astrocytes.

Sébastien Sultan , Liyi Li , Jonathan Moss , Francesco Petrelli , Frédéric Cassé
Neuron, 2015, 88 (5), pp.957-972. ⟨10.1016/j.neuron.2015.10.037⟩
Article dans une revue hal-02882611v1
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mTOR is essential for corticosteroid effects on hippocampal AMPA receptor function and fear memory

Hui Xiong , Frédéric Cassé , Yang Zhou , Ming Zhou , Zhi-Qi Xiong
Perceptual & Motor Skills: Learning & Memory, 2015, 22 (12), pp.577-583. ⟨10.1101/lm.039420.115⟩
Article dans une revue hal-03930835v1

mGlu5 receptors regulate synaptic sumoylation via a transient PKC-dependent diffusional trapping of Ubc9 into spines

Céline Loriol , Frédéric Cassé , Anouar Khayachi , Gwénola Poupon , Magda Chafai
Nature Communications, 2014, 5 (1), ⟨10.1038/ncomms6113⟩
Article dans une revue hal-03084688v1

mGlu5 receptors regulate synaptic sumoylation via a transient PKC-dependent diffusional trapping of Ubc9 into spines

Céline Loriol , Frédéric Cassé , Anouar Khayachi , Gwénola Poupon , Magda Chafai
Nature Communications, 2014, 5 (1), ⟨10.1038/ncomms6113⟩
Article dans une revue hal-03039214v1

Protein Sumoylation in Brain Development, Neuronal Morphology and Spinogenesis

Carole Gwizdek , Frédéric Cassé , Stéphane Martin
NeuroMolecular Medicine, 2013, 15 (4), pp.677-691. ⟨10.1007/s12017-013-8252-z⟩
Article dans une revue hal-03084691v1

Protein Sumoylation in Brain Development, Neuronal Morphology and Spinogenesis.

Carole Gwizdek , Frédéric Cassé , Stéphane Martin
NeuroMolecular Medicine, 2013, epub ahead of print. ⟨10.1007/s12017-013-8252-z⟩
Article dans une revue hal-00855033v1

Glutamate controls tPA recycling by astrocytes, which in turn influences glutamatergic signals.

Frédéric Cassé , Isabelle Bardou , Lydia Danglot , Aurélien Briens , Axel Montagne
Journal of Neuroscience, 2012, 32 (15), pp.5186-99. ⟨10.1523/JNEUROSCI.5296-11.2012⟩
Article dans une revue hal-00786439v1

Antibodies Preventing the Interaction of Tissue-Type Plasminogen Activator With N-Methyl- d -Aspartate Receptors Reduce Stroke Damages and Extend the Therapeutic Window of Thrombolysis

Richard Macrez , Pauline Obiang , Maxime Gauberti , Benoit Roussel , Amandine Baron
Stroke, 2011, 42 (8), pp.2315-2322. ⟨10.1161/STROKEAHA.110.606293⟩
Article dans une revue hal-03895712v1

Tissue-type plasminogen activator induces plasmin-dependent proteolysis of intracellular neuronal nitric oxide synthase

Amandine Baron , Yannick Hommet , Frédéric Cassé , Denis Vivien
Biology of the Cell, 2010, 102 (10), pp.539-547. ⟨10.1042/bc20100072⟩
Article dans une revue hal-03895701v1

NR2D-containing NMDA receptors mediate tissue plasminogen activator-promoted neuronal excitotoxicity

A Baron , A Montagne , Frédéric Cassé , S Launay , E Maubert
Cell Death and Differentiation, 2010, 17 (5), pp.860-871. ⟨10.1038/cdd.2009.172⟩
Article dans une revue hal-03895693v1

Mesenchymal stromal cells-derived exosomes: a new treatment for horse osteoarthritis?

Frédéric Cassé , Manon Jammes , Émilie Velot , Arnaud Bianchi , Fabrice Audigié
FSEV Meeting 2023, Société Française des vésicules extracellulaires, Nov 2023, On line, France
Communication dans un congrès hal-04347467v1

The secretome of equine bone marrow-derived mesenchymal stem cells enhanced regenerative phenotype of equine articular chondrocytes

Manon Jammes , Romain Contentin , Frédéric Cassé , Bastien Bourdon , Arnaud Bianchi
Journées de l'Ecole Doctorale Normande de Biologie Intégrative, Santé, Environnement (nBISE) - 24e édition, Jun 2022, Caen, France
Communication dans un congrès hal-03728083v1

Injection intra-articulaire d’un nanogel fonctionnalisé combinant des effets mécanique et biologique rémanents contre l’arthrose chez le cheval : étude de tolérance

A. Terlinden , Sandrine Jacquet , Aurélie Cullier , Frédéric Cassé , F. Legendre
50èmes Journées annuelles de l'AVEF, Nov 2022, Reims, France
Communication dans un congrès hal-04004349v1

Study of therapeutic potential of new functionalized nanogels against osteoarthritis by using equine cartilage organoid cultures

Aurélie Cullier , Frédéric Cassé , Florence Legendre , Romain Contentin , Seng Manivong
Journée Normande de Recherche Biomédicale, Jun 2022, Rouen, France
Communication dans un congrès hal-03722806v1

Functionalized Nanohydrogels decrease Inflammatory and Cartilage Degradation Biomarkers in an organoid model of horse cartilage tissue

Aurélie Cullier , Romain Contentin , Frédéric Cassé , Florence Legendre , Seng Manivong
XXIIIèmes Journées de l'Ecole Doctorale Normande de Biologie Intégrative Santé et Environnement, May 2021, CAEN, France
Communication dans un congrès hal-03229755v1