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Fernanda FONSECA

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#### **EDUCATION** **Habilitation à Diriger des Recherches (HDR).** Université de Technologie de Compiègne, 2010. Title : « Stabilization of bio functionalities trough an approach combining the understanding of mechanisms and the product-process engineering” **Ph.D. Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech),**2001**. Ph.D. thesis title: "Influence of cryoprotection and freezing on the acidification activity of thermopile lactic acid bacteria: relationships between biological and thermodynamic properties." **Master Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech), **1997**. M.Sc. thesis title: "Study of the freezing step on the activity of frozen lactic acid starters." Engineer** in **Chemical Engineering**, Montevideo University (Uruguay), **1996.** **SCIENTIFIC EXPERIENCE** ------------------------- **Research Director (since January 2015),** in Paris-Saclay Food and Bioproduct Engineering research unit (UMR 782 SayFood INRAE-AgroParisTech), Versailles-Grignon Centre, at the French National Institute for Agriculture, Food and Environment (INRAE), France. **Researcher Scientist** (**September 2001 – December 2014**), in the Joint Research Unit for Food Process Engineering and Microbiology (UMR 782 GMPA INRA-AgroParisTech), Versailles-Grignon Centre, Institut National de la Recherche Agronomique (INRA), France. **Main fields of expertise:**Biotechnology and process engineering: fermentation, formulation, and stabilization of bioproducts. - Bioproducts: micro-organisms, proteins, food products. - Stabilization processes: freezing and freeze-drying - Biophysical characterization of bioproducts (thermal analysis, freeze-drying microscopy, Fourier Transformed InfraRed microspectroscopy) **Research approach:** Focusing on biophysical and chemical behaviors of bioproducts during stabilization processes and by relating them to biological and functional responses, to identify and quantify the mechanisms governing the degradation and preservation of bioproducts during the stabilization processes. The ambition is also to find general principles for simplifying and rationalizing the development of newly formulated products and the stabilization process optimization, with particular attention to limiting the environmental impact.

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The Impact of varying cooling and thawing rates on the quality of cryopreserved human peripheral blood t cells

Jasmin Baboo , Peter Kilbride , Mike Delahaye , Stuart Milne , Fernanda Fonseca
Scientific Reports, 2019, 9 (1), pp.1-13. ⟨10.1038/s41598-019-39957-x⟩
Article dans une revue hal-02154617v1
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Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation

Fernanda Fonseca , Julie Meneghel , Stéphanie Cenard , Stéphanie Passot , G. John Morris
PLoS ONE, 2016, 11 (4), pp.e0152939. ⟨10.1371/journal.pone.0152939⟩
Article dans une revue hal-01565156v1
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A low temperature limit for life on Earth

Andrew Clarke , G. John Morris , Fernanda Fonseca , Benjamin J. Murray , Elizabeth Acton
PLoS ONE, 2013, 8 (6), ⟨10.1371/journal.pone.0066207⟩
Article dans une revue hal-01190603v1

Freezing injury: The special case of the sperm cell

G. John Morris , Elizabeth Acton , Benjamin J. Murray , Fernanda F. Fonseca
Cryobiology, 2012, 64 (2), pp.71-80. ⟨10.1016/j.cryobiol.2011.12.002⟩
Article dans une revue hal-02649267v1

Effect of controlled ice nucleation on primary drying stage and protein recovery in vials cooled in a modified freeze-dryer

Stéphanie Passot , Ioan-Cristian Trelea , Michele Marin , Miquel Galan , G. John Morris
Journal of Biomechanical Engineering, 2009, 131 (7), pp.074511:1-5. ⟨10.1115/1.3143034⟩
Article dans une revue hal-01537095v1

Rapidly cooled horse spermatozoa: loss of viability is due to osmotic imbalance during thawing, not intracellular ice formation

G. John Morris , K. Faszer , J. E. Green , D. Draper , B. W. W. Grout
Theriogenology, 2007, 68 (5), pp.804-812
Article dans une revue hal-02653848v1

The high viscosity encountered during freezing in glycerol solutions: effects on cryopreservation

G. John Morris , Martha Goodrich , Elizabeth Acton , Fernanda F. Fonseca
Cryobiology, 2006, 52 (3), pp.323-334. ⟨10.1016/j.cryobiol.2006.01.003⟩
Article dans une revue hal-02667959v1

Preserving bacteria with oligosaccharides and eco-friendly processes (Premium)

Andrea Gomez-Zavaglia , Stéphanie Passot , Caroline Pénicaud , Paula Castilho , Pedro Nuno Simões
55. Annual Meeting of the Society for Cryobiology, Jul 2018, Madrid, Spain. Elsevier Ltd, Cryobiology, 85 (December 2018), 2018, Cryobiology. ⟨10.1016/j.cryobiol.2018.10.203⟩
Poster de conférence hal-02266913v1

Interdependency of freezing and thawing rates on cryopreserved human t cells

Jasmin Baboo , Peter Kilbride , Mike Delahaye , Stuart Milne , Fernanda Fonseca
55. Annual Meeting of the Society for Cryobiology, Jul 2018, Madrid, Spain. Elsevier Ltd, Cryobiology, 85 (December 2018), 2018, Cryobiology. ⟨10.1016/j.cryobiol.2018.10.059⟩
Poster de conférence hal-02266903v1