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Fernanda FONSECA

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#### **EDUCATION** **Habilitation à Diriger des Recherches (HDR).** Université de Technologie de Compiègne, 2010. Title : « Stabilization of bio functionalities trough an approach combining the understanding of mechanisms and the product-process engineering” **Ph.D. Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech),**2001**. Ph.D. thesis title: "Influence of cryoprotection and freezing on the acidification activity of thermopile lactic acid bacteria: relationships between biological and thermodynamic properties." **Master Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech), **1997**. M.Sc. thesis title: "Study of the freezing step on the activity of frozen lactic acid starters." Engineer** in **Chemical Engineering**, Montevideo University (Uruguay), **1996.** **SCIENTIFIC EXPERIENCE** ------------------------- **Research Director (since January 2015),** in Paris-Saclay Food and Bioproduct Engineering research unit (UMR 782 SayFood INRAE-AgroParisTech), Versailles-Grignon Centre, at the French National Institute for Agriculture, Food and Environment (INRAE), France. **Researcher Scientist** (**September 2001 – December 2014**), in the Joint Research Unit for Food Process Engineering and Microbiology (UMR 782 GMPA INRA-AgroParisTech), Versailles-Grignon Centre, Institut National de la Recherche Agronomique (INRA), France. **Main fields of expertise:**Biotechnology and process engineering: fermentation, formulation, and stabilization of bioproducts. - Bioproducts: micro-organisms, proteins, food products. - Stabilization processes: freezing and freeze-drying - Biophysical characterization of bioproducts (thermal analysis, freeze-drying microscopy, Fourier Transformed InfraRed microspectroscopy) **Research approach:** Focusing on biophysical and chemical behaviors of bioproducts during stabilization processes and by relating them to biological and functional responses, to identify and quantify the mechanisms governing the degradation and preservation of bioproducts during the stabilization processes. The ambition is also to find general principles for simplifying and rationalizing the development of newly formulated products and the stabilization process optimization, with particular attention to limiting the environmental impact.

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Publications

782578

Using Raman spectroscopy to understand the osmotic stress response of lactic acid bacteria

Julie Meneghel , Jiawei Xu , Stéphanie Cenard , Dhruvananda Sockalingum , Stéphanie Passot
XXI. Journées du Groupe Français de Spectroscopies Vibrationnelles (GFSV), Jun 2015, Reims, France
Communication dans un congrès hal-01536574v1

Dynamic characterization of membrane biophysical properties to better understand bacterial tolerance to osmotic and cold stresses encountered during freezing

Julie Meneghel , Stéphanie Passot , Frederic Jamme , Matthieu Refregiers , Stéphanie Cenard
Microbial Stress: from molecules to systems, Nov 2015, Sitges, Spain
Communication dans un congrès hal-01535361v1

Initial measurement of the intracellular glass transition of a mammalian cell type under cryopreservation conditions

Fernanda Fonseca , Julie Meneghel , Stéphanie Cenard , Stéphanie Passot , D. Gilham
52nd Annual Meeting of the Society for Crryobiology, Jul 2015, Ostrava, Czech Republic. pp.538, ⟨10.1016/j.cryobiol.2015.10.008⟩
Communication dans un congrès hal-01535362v1

Freeze-drying modeling: prediction of spatial distribution of product quality

Stéphanie Passot , Fernanda Fonseca , Stéphanie Cenard , Ioan Cristian Tréléa
The Freeze Drying of Pharmaceuticals and Biologicals Conference, Sep 2014, Garmisch-Partenkirchen, Germany
Communication dans un congrès hal-01598172v1

Developing smart control strategies for the freeze-drying of bacteria

Stéphanie Passot , Fernanda Fonseca , Stéphanie Cenard , Carlos Vilas , Eva Balsa-Canto
ISL-FD: 5th International Conference on Lyophilization and Freeze Drying, 2012, Bologne, Italy
Communication dans un congrès hal-01598192v1

Developing smart control strategies for the freeze-drying of bacteria

Stéphanie Passot , Fernanda Fonseca , Stéphanie Cenard , C. Vilas , E. Balsa Canto
EFFoST Annual Meeting, Nov 2012, Montpellier, France
Communication dans un congrès hal-01628119v1

Real time optimization of freeze-drying of lactic acid bacteria (communication orale)

Stéphanie Passot , Carlos Vilas , Fernanda Fonseca , Stéphanie Cenard , Eva Balsa-Canto
SDDP2012 - 5th International Symposium on Spray Dried Dairy Products, Jun 2012, Saint-Malo, France
Communication dans un congrès hal-01598191v1

Biophysical and chemical responses of Lb. bulgaricus to freezing by infrared microspectroscopy

J. Gautier , Stéphanie Passot , Frédéric Jamme , Stéphanie Cenard , Fernanda Fonseca
7. Soleil User's Meeting, Jan 2012, Saclay, France
Communication dans un congrès hal-01628120v1

Modeling for risk assessment of quality failures: Application to microorganisms

Stéphanie S. Passot , Ines Douania , Stéphanie Cenard , Fernanda F. Fonseca , Ioan-Cristian I.-C. Trelea
Freeze Drying of Pharmaceuticals and Biologicals Conference, Sep 2010, Garmisch-Partenkirchen, Germany
Communication dans un congrès hal-02751750v1

Water in freeze-dried lactic acid bacteria: Friend or Foe

Stéphanie S. Passot , Stéphanie Cenard , Ioan-Cristian I.-C. Trelea , Ines Douania , Fernanda F. Fonseca
EuroFoodWater, Mar 2010, Reims, France
Communication dans un congrès hal-02818597v1

Stabilization of lactic acid bacteria : relevance of physical properties on formulation and process efficiency

Fernanda F. Fonseca , Stéphanie Cenard , Ioan-Cristian I.-C. Trelea , Michele Marin , Stéphanie S. Passot
X. Congreso Latinoamericano de Microbiologia et Hygiena de los Alimentos, Oct 2009, Punta del Este, Uruguay
Communication dans un congrès hal-02750465v1

Freezing : a critical step for freeze-drying cycle development and product stability

Stéphanie S. Passot , Ioan-Cristian I.-C. Trelea , Stéphanie S. Cenard , Fernanda F. Fonseca
2008 ISPE Conference : Lyophilisation: Scientific Issues of the Process and Introduction to a Risk-Based Approach, Dec 2008, Barcelona, Spain
Communication dans un congrès hal-02750962v1

Stabilisation of lactic acid bacteria: relevance of physical properties on formulation efficiency

Fernanda F. Fonseca , Stéphanie S. Passot , Stéphanie S. Cenard , Ioan-Cristian I.-C. Trelea , Michele Marin
1. Int. DOM Symposium on Microbial Formulation, 2007, Uppsala, Sweden
Communication dans un congrès hal-02813661v1

Mapping membrane fluidity by fluorescence anisotropy to better understand bacterial toloerance to osmotic and cold stresses encountered during freezing

Julie Meneghel , Stéphanie Passot , Stéphanie Cenard , Jiawei Xu , Matthieu Refregiers
52. Annual Meeting of the Society for Cryobiology, Jul 2015, Ostrava, Czech Republic, Czech Republic. Elsevier Ltd, Cryobiology, 71, pp.549, 2015, Cryobiology. ⟨10.1016/j.cryobiol.2015.10.052⟩
Poster de conférence hal-01536575v1

Freeze-drying of bacteria : state of the art and new perspectives

Stéphanie Passot , Ioan-Cristian Trelea , Stéphanie Cenard , Fernanda Fonseca
7. International Conference of Lyophilization and Freeze-Drying, Jul 2015, Barcelona, Spain. 2015
Poster de conférence hal-01535314v1

Towards a better understanding of cryotolerance mechanisms in bacteria

Fernanda Fonseca , Stéphanie Passot , Julie Meneghel , Stéphanie Cenard , P. Dumas
52. Annual Meeting of the Society for Cryobiology, Jul 2015, Ostrava, Czech Republic. Academic Press - Elsevier, Cryobiology, 71 (3), pp.548, 2015, Cryobiology
Poster de conférence hal-01536531v1

Towards the in situ study of bacterial single cells following freeze-drying using synchrotron radiation

Stéphanie Passot , Frederic Jamme , M. Refregiers , Stéphanie Cenard , Fernanda Fonseca
6. International Conference of Lyophilization and Freeze-Drying, Oct 2013, Sao Paulo, Brazil. 2013
Poster de conférence hal-01628107v1

Towards the measurement of membrane fluidity at subzero temperatures for improving cell cryopreservation

Stéphanie Passot , Frédéric Jamme , Matthieu Refregiers , J. Gautier , Stéphanie Cenard
European Conference on the Spectroscopy of Biological Molecules (ECSBM 15), Aug 2013, Oxford, United Kingdom. Biomedical Spectroscopy and Imaging, 3(3), 2013, Biomedical Spectroscopy and Imaging
Poster de conférence hal-01628117v1

Freeze-drying of lactic acid bacteria

Fernanda Fonseca , Stéphanie Cenard , Stéphanie Passot
Cryopreservation and Freeze-Drying Protocols, 1257, Humana Press Inc., 12 p., 2015, Methods in Molecular Biology, 978-1-4939-2192-8. ⟨10.1007/978-1-4939-2193-5_24⟩
Chapitre d'ouvrage hal-01535358v1