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Fernanda FONSECA

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#### **EDUCATION** **Habilitation à Diriger des Recherches (HDR).** Université de Technologie de Compiègne, 2010. Title : « Stabilization of bio functionalities trough an approach combining the understanding of mechanisms and the product-process engineering” **Ph.D. Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech),**2001**. Ph.D. thesis title: "Influence of cryoprotection and freezing on the acidification activity of thermopile lactic acid bacteria: relationships between biological and thermodynamic properties." **Master Degree** in **Biotechnology and Process Engineering**, Institut National Agronomique Paris-Grignon (AgroParisTech), **1997**. M.Sc. thesis title: "Study of the freezing step on the activity of frozen lactic acid starters." Engineer** in **Chemical Engineering**, Montevideo University (Uruguay), **1996.** **SCIENTIFIC EXPERIENCE** ------------------------- **Research Director (since January 2015),** in Paris-Saclay Food and Bioproduct Engineering research unit (UMR 782 SayFood INRAE-AgroParisTech), Versailles-Grignon Centre, at the French National Institute for Agriculture, Food and Environment (INRAE), France. **Researcher Scientist** (**September 2001 – December 2014**), in the Joint Research Unit for Food Process Engineering and Microbiology (UMR 782 GMPA INRA-AgroParisTech), Versailles-Grignon Centre, Institut National de la Recherche Agronomique (INRA), France. **Main fields of expertise:**Biotechnology and process engineering: fermentation, formulation, and stabilization of bioproducts. - Bioproducts: micro-organisms, proteins, food products. - Stabilization processes: freezing and freeze-drying - Biophysical characterization of bioproducts (thermal analysis, freeze-drying microscopy, Fourier Transformed InfraRed microspectroscopy) **Research approach:** Focusing on biophysical and chemical behaviors of bioproducts during stabilization processes and by relating them to biological and functional responses, to identify and quantify the mechanisms governing the degradation and preservation of bioproducts during the stabilization processes. The ambition is also to find general principles for simplifying and rationalizing the development of newly formulated products and the stabilization process optimization, with particular attention to limiting the environmental impact.

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Insights into lactic acid bacteria cryoresistance using FTIR micro-spectroscopy

Amélie Girardeau , Stephanie Passot , Julie Meneghel , Stéphanie Cenard , Pascale Lieben
Analytical and Bioanalytical Chemistry, 2022, 414 (3), pp.1425-1443. ⟨10.1007/s00216-021-03774-x⟩
Article dans une revue hal-03552585v1
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The transfer temperature from slow cooling to cryogenic storage is critical for optimal recovery of cryopreserved mammalian cells

Peter Kilbride , Julie Meneghel , Fernanda Fonseca , John Morris
PLoS ONE, 2021, 16 (11), pp.e0259571. ⟨10.1371/journal.pone.0259571⟩
Article dans une revue hal-03446319v1

FTIR micro-spectroscopy using synchrotron-based and thermal source-based radiation for probing live bacteria

Julie Meneghel , Stéphanie Passot , Frédéric Jamme , Stéphane Lefrançois , Pascale Lieben
Analytical and Bioanalytical Chemistry, 2020, 412 (26), pp.7049-7061. ⟨10.1007/s00216-020-02835-x⟩
Article dans une revue hal-03004447v1
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Physical events occurring during the cryopreservation of immortalized human T cells

Julie Meneghel , Peter Kilbride , John G. Morris , Fernanda Fonseca
PLoS ONE, 2019, 14 (5), pp.e0217304. ⟨10.1371/journal.pone.0217304⟩
Article dans une revue hal-02154629v1
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The Impact of varying cooling and thawing rates on the quality of cryopreserved human peripheral blood t cells

Jasmin Baboo , Peter Kilbride , Mike Delahaye , Stuart Milne , Fernanda Fonseca
Scientific Reports, 2019, 9 (1), pp.1-13. ⟨10.1038/s41598-019-39957-x⟩
Article dans une revue hal-02154617v1

Biophysical characterization of the Lactobacillus delbrueckii subsp. bulgaricus membrane during cold and osmotic stress and its relevance for cryopreservation

Julie Meneghel , Stéphanie Passot , Sébastien Dupont , Fernanda Fonseca
Applied Microbiology and Biotechnology, 2017, 101 (4), pp.1427 - 1441. ⟨10.1007/s00253-016-7935-4⟩
Article dans une revue hal-01559240v1
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Determination of Intracellular Vitrification Temperatures for Unicellular Micro Organisms under Conditions Relevant for Cryopreservation

Fernanda Fonseca , Julie Meneghel , Stéphanie Cenard , Stéphanie Passot , G. John Morris
PLoS ONE, 2016, 11 (4), pp.e0152939. ⟨10.1371/journal.pone.0152939⟩
Article dans une revue hal-01565156v1
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Draft Genome Sequence of Lactobacillus delbrueckii subsp. bulgaricus CFL1, a Lactic Acid Bacterium Isolated from French Handcrafted Fermented Milk

Julie Meneghel , Eric Dugat-Bony , Françoise Irlinger , Valentin Loux , Marie Vidal
Genome Announcements, 2016, 4 (2), pp.e00052-16. ⟨10.1128/genomeA.00052-16⟩
Article dans une revue hal-01511494v1
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Draft Genome Sequence of Lactobacillus delbrueckii subsp. bulgaricus CFL1, a Lactic Acid Bacterium Isolated from French Handcrafted Fermented Milk

Julie Meneghel , Eric Dugat-Bony , Françoise Irlinger , Valentin Loux , Marie Vidal
Genome Announcements, 2016, 4 (2), pp.e00052-16. ⟨10.1128/genomea.00052-16⟩
Article dans une revue hal-03649172v1
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Optimising controlled rate freezing protocols

Julie Meneghel , Peter Kilbride , Fernanda Fonseca , G.John Morris
Manufacturing ATMPs – moving from approvable to commercial success, Sep 2018, London, United Kingdom
Poster de conférence hal-02999268v1