Nombre de documents



Etienne DAGUE


CNRS Researcher, PhD-HDR, PharmD

Researcher unique identifier ORCID:0000-0003-3290-9166 full ID:

Date of birth: 08/10/1978

URL for web site:



7 av du Colonel Roche

31000 TOULOUSE, France

0033 5 61 33 78 41




2001:           Master in Biological and Medical Sciences, France


2003:           Master in Chemistry and Microbiology of Water University of Lorraine, France


2003:           Pharmaceutical Doctor University of Lorraine, France


2006:           PhD of University of Lorraine, France


2012:           Habilitation à Diriger la Recherche, University of Toulouse, France






2007- Current Position: Researcher at CNRS, France


Laboratory of Architecture and Analysis of Systems, Engineering for Life Science (ELIA), Toulouse, France


2011- 2016: Group leader at ITAV-CNRS-University of Toulouse


ITAV: Advanced technologies for life sciences, Group Nanocardiology and cancerous cells biophysic (NBC)






2001- 2002: Position held: Internship as associate pharmacist at Neufchâteau Hospital, France


2006 - 2007: Position held: Associate researcher, Belgium


Catholic University of Louvain/ unit of Interface Chemsitry (CIFA), Louvain-la-Neuve, Belgium






I am the author of 56 articles published in international scientific peer reviewed journal. I have an H index of 22, my work has ben cited more than 1430 times (Google scholar). I have been invited to 7 international conferences to give invited lectures, presented 20 oral communications and more than 30 posters in international scientific conferences.


Full list at this address:






2004: Improving the Human Potential (European Union) IHP fellowship to participate to BIO-IMED II : Biofilms in industry, medicine end environmental biotechnology : the technology (9-14 August 2004 Galway, Ireland).


2007: University of Lorraine, Nancy France: PhD Thesis award


         Lorraine academy of science, Nancy, France: PhD Thesis award


2008:  National academy of Pharmacy, Paris, France: Pharmaceutical Sciences award for my PhD thesis.


2010:  Indo-French Centre for the Promotion of Advanced Research fellowship, and invited lecture at Host pathogen interaction in respiratory infectious diseases (HOPE in Red) (Bangalore, India, 11-13 October 2010)


2011:  JPK Berlin, Germany fellowship and invited lecture at NanoBioView Scanning probe microscopy and Optical tweezers in life sciences (Germany, Berlin, 5-6 October 2011)


2013:  American Society of Microbiology, Washington DC, USA, Georges McCracken Infectious Disease Fellow


2014:  ICNANO Mexcio, Mexico fellowhip and plenary lecture at ICANano 2014 International congress on application of nanotechnology (Mexico, Mexico 30 September-2 October)


2015: XIVème colloque de la Société Française des Microscopies (Nice, France 30 juin-3 juillet)


Formosa C., Dague E., Comprehension of the mechanisms of actions of antimicrobial drugs using bionantotechnologies (invited plenary lecture)


         11ème journée du cancéropole Grand Sud Ouest (Bordeaux, France, 5-6 novembre 2015)


Pillet F., Gibot L., Madi M., Rols P-P., Dague E., Atomic Force Microscopy: a tool to study native dermis and 3D cellular models (Poster award)


         NanoBioMed 2015 (Barcelone, Spain, 18-20 nov 2015)


Dague E. Biomedical Applications of Atomic Force Microscopy (Invited keynote lecture)


2016: Asgard fellow, funding from institute de France en Norvège for collaboration






2003-2006: 1 1st year master student, 2 2nd year technical classes.


University of Lorraine/ LCPME UMR 7564


2007-2017:  4 Postdocs/ 7 PhDs/ 5 Master Students


University of Toulouse/ LAAS-CNRS or ITAV-CNRS/ group NBS or NBC






2003-2006:    192 h, Informatics (2nd year pharmacy, Nancy, France), Hematology and Parasitology (1st year technical classes, Nancy, France)


2008-2009:    4 h bibliography analysis (M2R Microbiology, Toulouse, France)


2009-2010:    14 h of practical classes Bio-AFM, 5th year of INSA Toulouse (Engineering school)


2011-2015:    56 h of practical classes Bio-AFM, 5th year of INSA Toulouse (Engineering school)


                   Lecture NanoBiotechnology, Master Diagnostics in Microbiology (University of Toulouse)


2011-2014:    16 h lecture on NanoBiotechnology to master student BioEngineering (University of Toulouse)


2013:           4 h Invited lectureFrom single cell to single molecule, AFM in life sciences” for PhD students within the fields Biophysics, Biotechnology, Pharmacy, Medicine or related fields. NTNU, Trondheim, Norway.






2013:           Local thematic day on “Atomic force Microscopy: theme and variations” (16 May 2013,   University of Toulouse, France)


2013:           International INSERM workshop “Cellular and molecular Nanomechanics as probed by Atomic     Force Microscopy” (23 May 2013, Paris, France)


2014:           National workshop on local probe microscopies (17-21 March 2014, Montauban, France)


2016            - NanoInBio advances for life and materials sciences (31 may- 5 june 2016 Guadeloupe, France)


                   - Scientific committee of ICONAN, International Conference on Nanomedicine and          Nanobiotechnology (28-30 september 2016, Paris France)






2001-2003:    member of the faculty and pedagogic council: Pharmacy School, Nancy France


2002-2004:    member of the research council of pharmacy School, Nancy,


                   member of the research council of Lorraine University and member of the student life office


2004-2006:    member of the research council of pharmacy School, Nancy,


                   member of the study and student life council of Lorraine University, Nancy, France


2011- 2013:   elected member of the lab council: LAAS-CNRS UPR 8001.


2010-2012:    nominated member of the lab council: ITAV-CNRS UMS 3039


2012-2016:    full member of the lab council: ITAV-CNRS USR 3505






2009:           ANR P3N: evaluation of 3 projects


2010:           ANR JCJC SIMI 10: evaluation of 1 project


2012-          :PlosOne academic editor


2012; 2014:   Fonds National pour la Recherche Scientifique (Belgium): evaluator


2014:           Evaluator for Région Ile de France and for Région Franche-Comté scientific council.


2010-          :Reviewer for Physical chemistry chemical physics, Journal of Molecular Recognition, Journal of Structural Biology, Biophysical Journal, Biomacromolecules, Biofouling, Biomaterials, Biotechnology Advances, Langmuir, Journal of Biomechanics, JACS, Future medicine: nanomedicine, Micron, BBA biomembrane, PlosOne, Future medicine, colloids and surfaces B, Nanoscale, Trends in Microbiology






2006-2012:    Société Française pour l’Avancée des Sciences (AFAS)


2013-        :  American Society for Microbiology (ASM)






2010-2012: APTATIPS: ANR NanoInnov C’Nano Grand Sud Ouest : 20 keuros


2010-2013: THERMOBIOAFM: risks at the physics, chemistry and biology interface, CNRS: 50 keuros


2012-2015: AFMYST: ANR JCJC SIMI 5:  182 keuros


2013-2015: BACT’EAU: Région Midi Pyrénée: 28 keuros


2013-2016: MIFACARD: Région Midi Pyrénée and Fondation Recherche Médicale: 215 keuros.


2015-2017: SMiRCH: IDEX transversality: 50 Keuros


2015-2019: NanoPalpation: ECOS Nord, France-Mexic cooperation project: 8 missions


2016-2017: Lallwall 1 et 2: Contrat industriel Société Lallemand: 57 keuros


2017     :AURORA exchange between France and Norway funded by Campus France


2018-2021: GRAPHICS ANR PRC CE 11: 350 keuros


Article dans une revue32 documents

  • Arthur Gaveau, Clémence Coetsier, Christine Roques, Patrice Bacchin, Etienne Dague, et al.. Bacteria transfer by deformation through microfiltration membrane. Journal of Membrane Science, Elsevier, 2017, 523, pp.446-455. 〈10.1016/j.memsci.2016.10.023〉. 〈hal-01451400〉
  • Cécile Formosa-Dague, Raphaël E. Duval, Etienne Dague. Cell biology of microbes and pharmacology of antimicrobial drugs explored by Atomic Force Microscopy. Seminars in Cell & Developmental Biology, 2017, 12p. 〈10.1016/j.semcdb.2017.06.022〉. 〈hal-01651186〉
  • Marion Schiavone, Sebastien Dejean, Nathalie Sieczkowski, Mathieu Castex, Etienne Dague, et al.. Integration of Biochemical, Biophysical and Transcriptomics Data for Investigating the Structural and Nanomechanical Properties of the Yeast Cell Wall. Frontiers in Microbiology, Frontiers Media, 2017, 8, pp.1806 〈10.3389/fmicb.2017.01806〉. 〈hal-01698344〉
  • G. Smolyakov, Cécile Formosa-Dague, C. Severac, Raphaël Duval, Etienne Dague. High speed indentation measures by FV, QI and QNM introduce a new understanding of bionanomechanical experiments. Micron, Elsevier, 2016, 85, pp.8-14. 〈10.1016/j.micron.2016.03.002〉. 〈hal-01496648〉
  • Flavien Pillet, Cécile Foromsa-Dague, Houda Baaziz, Etienne Dague, Marie-Pierre Rols. Cell wall as a target for bacteria inactivation by pulsed electric fields. Scientific Reports, Nature Publishing Group, 2016, 〈10.1038/srep19778〉. 〈hal-01552815〉
  • Georges Smolyakov, Bénédicte Thiebot, Clément Campillo, Sid Labdi, Childerick Severac, et al.. Elasticity, Adhesion, and Tether Extrusion on Breast Cancer Cells Provide a Signature of Their Invasive Potential. ACS Applied Materials & Interfaces, Washington, D.C. : American Chemical Society, 2016, 8 (41), pp.27426 - 27431. 〈10.1021/acsami.6b07698〉. 〈hal-01552762〉
  • Marion Schiavone, Cécile Formosa-Dague, Carolina Elsztein, Marie-Ange Teste, Helene Martin-Yken, et al.. Evidence for a Role for the Plasma Membrane in the Nanomechanical Properties of the Cell Wall as Revealed by an Atomic Force Microscopy Study of the Response of Saccharomyces cerevisiaeto Ethanol Stress. Applied and Environmental Microbiology, American Society for Microbiology, 2016, 82, pp.4789 - 4801. 〈10.1128/AEM.01213-16〉. 〈hal-01552795〉
  • Cécile Formosa-Dague, M. Herold, C Vidaillac, R. E. Duval, Etienne Dague. Unravelling of a mechanism of resistance to colistin in Klebsiella pneumoniae using atomic force microscopy. Journal of Antimicrobial Chemotherapy, Oxford University Press (OUP), 2015, 70 (8), pp.2261-2270. 〈10.1093/jac/dkv118〉. 〈hal-01553126〉
  • Cécile Formosa-Dague, V Lachaize, C Galés, M P Rols, Hélène Martin-Yken, et al.. Mapping HA-tagged protein at the surface of living cells by atomic force microscopy. Journal of Molecular Recognition, Wiley, 2015, 28 (1), pp.1-9. 〈10.1002/jmr.2407〉. 〈hal-01169622〉
  • Helene Yken, Ran Liu, Cécile Formosa-Dague, Marion Schiavone, Etienne Dague, et al.. Combining Atomic Force Microscopy and genetics to investigate the role of Knr4 in Saccharomyces cerevisiae sensitivity to K9 Killer toxin. Letters in Applied NanoBioScience, 2015, In Press. 〈hal-01269076〉
  • Iuliana V. Ene, Louise Brown, Marion Schiavone, Keunsook Lee, Hélène Martin-Yken, et al.. Cell Wall Remodeling Enzymes Modulate Fungal Cell Wall Elasticity and Osmotic Stress Resistance. mBio, American Society for Microbiology, 2015, 6 (4), pp.e00986-15. 〈10.1128/mBio.00986-15〉. 〈hal-01553096〉
  • Iuliana Ene, Louise Walker, Marion Schiavone, Keunsook K. Lee, Helene Yken, et al.. Cell wall remodelling enzymes modulate fungal cell wall elasticity and osmotic stress resistance. mBio, American Society for Microbiology, 2015, 6 (4), 〈10.1128/mBio.00986-15〉. 〈hal-01269162〉
  • Véronique Lachaize, Cécile Formosa-Dague, Georgiy Smolyakov, Celine Guilbeau-Frugier, Céline Galés, et al.. Atomic Force Microscopy: an innovative technology to explore cardiomyocyte cell surface in cardiac physio/pathophysiology. Letters in Applied NanoBioScience, 2015, 4 (4), pp.321-324. 〈〉. 〈hal-01552834〉
  • Ran Liu, Cécile Formosa-Dague, Adilia Dagkessamanskaia, Etienne Dague, Jean Marie François, et al.. Combining atomic force microscopy and genetics to investigate the role of KNR4 inSaccharomyces Cerevisiae sensitivity to K9 killer toxin. Letters in Applied NanoBioScience, 2015, 4 (4), pp.306-315. 〈hal-01553082〉
  • Cécile Formosa-Dague, Flavien Pillet, Marion Schiavone, Raphaël E. Duval, Laurence Ressier, et al.. Generation of living cell arrays for atomic force microscopy studies. Nature Protocols, Nature Publishing Group, 2015, 10 (1), pp.199-204. 〈10.1038/nprot.2015.004〉. 〈hal-01495201〉
  • Marion Schiavone, Nathalie Sieczkowski, Mathieu Castex, Etienne Dague, Jean Marie François. Effects of the strain background and autolysis process on the composition and biophysical properties of the cell wall from two different industrial yeasts. FEMS Yeast Research, Oxford University Press (OUP), 2015, 15 (2), pp.12 〈10.1093/femsyr/fou012〉. 〈hal-01553141〉
  • Cécile Formosa-Dague, Marion Schiavone, Anita Boisrame, Mathias Richard, Raphael Duval, et al.. Multiparametric imaging of adhesive nanodomains at the surface of Candida albicans by atomic force microscopy. Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2015, 11 (1), pp.57-65. 〈10.1016/j.nano.2014.07.008〉. 〈hal-01553144〉
  • Etienne Dague, Gaël Genet, Véronique Lachaize, Céline Guilbeau-Frugier, Jérémy Fauconnier, et al.. Atomic force and electron microscopic-based study of sarcolemmal surface of living cardiomyocytes unveils unexpected mitochondrial shift in heart failure.. Journal of Molecular and Cellular Cardiology, Elsevier, 2014, 74C, pp.162-172. 〈10.1016/j.yjmcc.2014.05.006〉. 〈inserm-01016102〉
  • Vitor Cabral, Sadri Znaidi, Louise A. Walker, Helene Yken, Etienne Dague, et al.. Targeted changes of the cell wall proteome influence [i]Candida albican[/i]s ability to form single- and multi-strain biofilms. PLoS Pathogens, Public Library of Science, 2014, 10 (12), pp.e1004542. 〈10.1371/journal.ppat.1004542〉. 〈hal-01204415〉
  • Louise Chopinet-Mayeux, C. Formosa, M. P. Rols, R. E. Duval, Etienne Dague. Imaging living cells surface and quantifying its properties at high resolution using AFM in QI (TM) mode. Micron, Elsevier, 2013, 48, pp.26-33. 〈10.1016/j.micron.2013.02.003〉. 〈hal-01493934〉
  • Cécile Formosa-Dague, Marion Schiavone, Hélène Martin-Yken, J.M. François, Raphael Duval, et al.. Nanoscale Effects of Caspofungin against Two Yeast Species, Saccharomyces cerevisiae and Candida albicans. Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2013, 57 (8), pp.3498 - 3506. 〈10.1128/AAC.00105-13〉. 〈hal-01268080〉
  • Jean Marie Francois, Cécile Formosa-Dague, Marion Schiavone, Flavien Pillet, Helene Yken, et al.. Use of atomic force microscopy (AFM) to explore cell wall properties and response to stress in the yeast Saccharomyces cerevisiae. Current Genetics, Springer Verlag, 2013, 59 (4), pp.187 - 196. 〈10.1007/s00294-013-0411-0〉. 〈hal-01268470〉
  • Doan Le, Thi-Ly Tran, Marie-Pierre Duviau, Mickael Meyrand, Yann Guerardel, et al.. Unraveling the role of surface mucus-binding protein and pili in muco-adhesion of lactococcus lactis. PLoS ONE, Public Library of Science, 2013, 8 (11), 〈10.1371/journal.pone.0079850〉. 〈hal-01204286〉
  • Anne Beauvais, Silvia Bozza, Olaf Kniemeyer, Cécile Formosa-Dague, Viviane Balloy, et al.. Deletion of the α-(1,3)-glucan synthase genes induces a restructuring of the conidial cell wall responsible for the avirulence of Aspergillus fumigatus.. PLoS Pathogens, Public Library of Science, 2013, 9 (11), pp.e1003716. 〈10.1371/journal.ppat.1003716〉. 〈pasteur-01450713〉
  • Gaël Genet, Céline Guilbeau-Frugier, Benjamin Honton, Etienne Dague, Michael Schneider, et al.. Ephrin-B1 is a novel specific component of the lateral membrane of the cardiomyocyte and is essential for the stability of cardiac tissue architecture cohesion.. Circulation Research, American Heart Association, 2012, 110 (5), pp.688-700. 〈10.1161/CIRCRESAHA.111.262451〉. 〈inserm-00755171〉
  • C. Formosa, M. Grare, E. Jauvert, A. Coutable, J. B. Regnouf-De-Vains, et al.. Nanoscale analysis of the effects of antibiotics and CX1 on a Pseudomonas aeruginosa multidrug-resistant strain. Scientific Reports, Nature Publishing Group, 2012, 2 (1), 〈10.1038/srep00575〉. 〈hal-01650390〉
  • Cécile Formosa-Dague, Marion Grare, Raphaël E. Duval, Etienne Dague. Nanoscale effects of antibiotics on P. aeruginosa. Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2012, 8 (1), pp.12 - 16. 〈10.1016/j.nano.2011.09.009〉. 〈hal-01650395〉
  • Eric Jauvert, Etienne Dague, Marjorie Severac, Laurence Ressier, Anne-Marie Caminade, et al.. Probing single molecule interactions by AFM using bio-functionalized dendritips. Sensors and Actuators B: Chemical, Elsevier, 2012, 168, pp.436 - 441. 〈10.1016/j.snb.2012.04.048〉. 〈hal-01268327〉
  • Hélène Lalo, Cédric Ayela, Etienne Dague, Christophe Vieu, Karsten Haupt. Nanopatterning molecularly imprinted polymers by soft lithography: a hierarchical approach. Lab on a Chip, Royal Society of Chemistry, 2010, issue 10, pp. 1316 - 1318,. 〈10.1039/b924315a〉. 〈hal-00493035〉
  • M. Grare, Etienne Dague, M. Mourer, J.-B. Regnouf-De-Vains, C. Finance, et al.. Microélectrophorèse et microscopie à force atomique: deux nouveaux outils d'évaluation de l'effet pariétal d'antibactériens. Pathologie Biologie, Elsevier Masson, 2007, 55 (10), pp.465 - 471. 〈10.1016/j.patbio.2007.08.001〉. 〈hal-01651226〉
  • Etienne Dague, Fabien Gaboriaud, Jérôme Duval, Frédéric Jorand, Fabien Thomas. Probing surface structures of Shewanella spp. by microelectrophoresis. Biophysical Journal, Biophysical Society, 2006, 90, pp.2612. 〈10.1529/biophysj.105.068205〉. 〈hal-00090765〉
  • Fabien Gaboriaud, Etienne Dague, Sidney Bailet, Frédéric Jorand, Jérôme Duval, et al.. Multiscale dynamics of the cell envelope of Shewanella putrefaciens as a response to pH change. Colloids and Surfaces, 2006, 52, pp.107. 〈hal-00112240〉

Communication dans un congrès2 documents

  • Hélène Yken, Cécile Formosa-Dague, Marion Schiavone, Jean-Marie Francois, Etienne Dague. Drug resistance and adhesion: a closer look at the dark side of the wall. 27. International Conference on Yeast Genetics and Molecular Biology (ICYGMB), Sep 2015, Levico Trente, Italy. Wiley-Blackwell, 27th International Conference on Yeast Genetics and Molecular Biology (ICYGMB), 32, 2 p., 2015, Yeast. 〈hal-01269495〉
  • Hélène Lalo, Etienne Dague, Christophe Vieu, Karsten Haupt, Cédric Ayela. Biomimetic nanopatterns achieved by combining Molecularly Imprinted Polymers with Soft Lithography. MRS Fall meeting 2009, Nov 2009, Boston, United States. 1 page, 2009. 〈hal-00493038〉

Poster2 documents

  • Helene Yken, Cécile Formosa-Dague, Marion Schiavone, Etienne Dague. The dark side of the wall: atomic force microscopy revelations on drug resistance and adhesion. 7. FEBS Advanced Lecture Course on "Human Fungal Pathogens - Molecular Mechanisms of Host-Pathogen Interactions and Virulence", May 2017, La Colle sur Loup, France. 2017. 〈hal-01608703〉
  • Helene Yken, Cécile Formosa-Dague, Marion Schiavone, Etienne Dague. Stress, Drug Resistance, Adhesion: the dark side of the wall.. NanoInBio Advances for life and materials science , May 2016, Le Gosier, France. 2016. 〈hal-01604110〉

Chapitre d'ouvrage1 document