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Clément Nizak

My current research focuses on enzymes and antibodies as experimental models to tackle fundamental questions related to bio-molecule function and evolution. Our experiments are based on droplet microfluidics, phage display and high-throughput DNA sequencing technologies. Our approaches are strongly motivated by computational analysis and theory.

I work at ESPCI Paris in collaboration with Andrew Griffiths (Laboratory of Biochemistry) and at CIRB-College de France in collaboration with Olivier Rivoire (Statistical Biology).

I teach part of the Systems Biology course at ESPCI and the ICFP Masters program of ENS Paris.

Journal articles22 documents

  • Steven Schulz, Sébastien Boyer, Matteo Smerlak, Simona Cocco, Rémi Monasson, et al.. Parameters and determinants of responses to selection in antibody libraries. PLoS Computational Biology, Public Library of Science, 2021, 17 (3), pp.e1008751. ⟨10.1371/journal.pcbi.1008751⟩. ⟨hal-03190048⟩
  • Johan Fenneteau, Dany Chauvin, Andrew D. Griffiths, Clément Nizak, Janine Cossy. Synthesis of new hydrophilic rhodamine based enzymatic substrates compatible with droplet-based microfluidic assays. Chemical Communications, Royal Society of Chemistry, 2017, 53 (39), pp.5437-5440. ⟨10.1039/c7cc01506b⟩. ⟨hal-02104894⟩
  • Klaus Eyer, Raphaël C L Doineau, Carlos E Castrillon, Luis Briseño-Roa, Vera Menrath, et al.. Single-cell deep phenotyping of IgG-secreting cells for high-resolution immune monitoring. Nature Biotechnology, Nature Publishing Group, 2017, 35 (10), pp.977 - 982. ⟨10.1038/nbt.3964⟩. ⟨hal-01639647⟩
  • Sébastien Boyer, Dipanwita Biswas, Anandakumar Soshee, Natate Scaramozzino, Clément Nizak, et al.. Hierarchy and extremes in selections from pools of randomized proteins. Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2016, ⟨10.1073/pnas.1517813113⟩. ⟨hal-01301847⟩
  • Darja Dubravcic, Minus van Baalen, Clément Nizak. An evolutionarily significant unicellular strategy in response to starvation in social amoebae Dictyostelium social amoebae [version 2; referees: 2 approved]. F1000Research, Faculty of 1000, 2014, 3, pp.133. ⟨10.12688/f1000research.4218.2⟩. ⟨hal-02392322⟩
  • Purvi Jain, Anandakumar Soshee, S.Shankara Narayanan, Jadab Sharma, Christian Girard, et al.. Selection of Arginine-Rich Anti-Gold Antibodies Engineered for Plasmonic Colloid Self-Assembly. Journal of Physical Chemistry C, American Chemical Society, 2014, 10.1021/jp502118n. ⟨10.1021/jp502118n⟩. ⟨hal-00983923v3⟩
  • Souvik Modi, S. Haider, Clément Nizak, Yamuna Krishnan. Recombinant antibody mediated delivery of organelle-specific DNA pH sensors along endocytic pathways. Nanoscale, Royal Society of Chemistry, 2014, 6 (2), pp.1144-52. ⟨10.1039/c3nr03769j⟩. ⟨hal-00959788⟩
  • Anandakumar Soshee, Stefan Zürcher, N. Spencer, A. Halperin, Clément Nizak. General in vitro method to analyze the interactions of synthetic polymers with human antibody repertoires. Biomacromolecules, American Chemical Society, 2014, 15 (1), pp.113-21. ⟨10.1021/bm401360y⟩. ⟨hal-00959789⟩
  • Darja Dubravcic, Minus van Baalen, Clément Nizak. An evolutionarily significant unicellular strategy in response to starvation stress in Dictyostelium social amoebae. F1000Research, Faculty of 1000, 2014, 3, pp.133. ⟨10.12688/f1000research.4218.1⟩. ⟨hal-01064077⟩
  • Monica Iosin, Teodora Scheul, Clément Nizak, Olivier Stephan, Simion Astilean, et al.. Laser microstructuration of three-dimensional enzyme reactors in microfluidic channels. Microfluidics and Nanofluidics, Springer Verlag, 2011, 10 (3), pp.685-690. ⟨10.1007/s10404-010-0698-9⟩. ⟨hal-00728055⟩
  • Ole Vielemeyer, Clément Nizak, Ana Joaquina Jimenez, Arnaud Echard, Bruno Goud, et al.. Characterization of single chain antibody targets through yeast two hybrid.. BMC Biotechnology, BioMed Central, 2010, 10 (1), pp.59. ⟨10.1186/1472-6750-10-59⟩. ⟨inserm-00617227⟩
  • Sandrine Adiba, Clément Nizak, Minus van Baalen, Erick Denamur, Frantz Depaulis. From Grazing Resistance to Pathogenesis: The Coincidental Evolution of Virulence Factors. PLoS ONE, Public Library of Science, 2010, 5 (8), pp.e11882. ⟨10.1371/journal.pone.0011882⟩. ⟨hal-00528524⟩
  • S. Moutel, A. El Marjou, O. Vielemeyer, Clément Nizak, P. Benaroch, et al.. A multi-Fc-species system for recombinant antibody production. BMC Biotechnology, BioMed Central, 2009, pp.PMID: 19245715. ⟨hal-00565195⟩
  • O. Vielemeyer, H. Yuan, S. Moutel, R. Saint-Fort, D. Tang, et al.. Direct selection of monoclonal phosphospecific antibodies without prior phosphoamino acid mapping. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2009, pp.PMID: 19473967. ⟨hal-00565193⟩
  • Clément Nizak, Rj Fitzhenry, Rh Kessin. Exploitation of other social amoebae by Dictyostelium caveatum. PLoS ONE, Public Library of Science, 2007, pp.PMID: 17299592. ⟨hal-00565197⟩
  • E. del Nery, S. Miserey-Lenkei, T. Falguières, Clément Nizak, Ludger Johannes, et al.. Rab6A and Rab6A' GTPases play non-overlapping roles in membrane trafficking. Traffic, Wiley, 2006, pp.PMID: 16536738. ⟨hal-00565201⟩
  • R. Sannerud, M. Marie, Clément Nizak, Ha Dale, Karin Pernet-Gallay, et al.. Rab1 defines a novel pathway connecting the pre-Golgi intermediate compartment with the cell periphery. Molecular Biology of the Cell, American Society for Cell Biology, 2006, pp.PMID: 16421253. ⟨hal-00565238⟩
  • Clément Nizak, S. Moutel, Bruno Goud, Franck Perez. Selection and application of recombinant antibodies as sensors of rab protein conformation. Methods in Enzymology, Elsevier, 2005, pp.PMID: 16473583. ⟨hal-00565236⟩
  • Clément Nizak, R. Sougrat, F. Jollivet, A. Rambourg, Bruno Goud, et al.. Golgi inheritance under a block of anterograde and retrograde traffic. Traffic, Wiley, 2004, pp.PMID: 15030570. ⟨hal-00565237⟩
  • Clément Nizak, S. Martin-Lluesma, S. Moutel, A. Roux, Te Kreis, et al.. Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo. Traffic, Wiley, 2003, pp.PMID: 14617357. ⟨hal-00565239⟩
  • Clément Nizak, S. Monier, E. del Nery, S. Moutel, Bruno Goud, et al.. Recombinant antibodies to the small GTPase Rab6 as conformation sensors. Science, American Association for the Advancement of Science (AAAS), 2003, pp.PMID: 12738866. ⟨hal-00565240⟩
  • Franck Perez, Karin Pernet-Gallay, Clément Nizak, Hv Goodson, Te Kreis, et al.. CLIPR-59, a new trans-Golgi/TGN cytoplasmic linker protein belonging to the CLIP-170 family. Journal of Cell Biology, Rockefeller University Press, 2002, pp.PMID: 11854307. ⟨hal-00565241⟩

Book sections1 document

  • Sandrine Moutel, Clément Nizak, Franck Perez. Selection and Use of Intracellular Antibodies. Methods in molecular biology, pp.491-503, 2018. ⟨hal-02104898⟩

Preprints, Working Papers, ...1 document

  • Steven Schulz, Sébastien Boyer, Matteo Smerlak, Simona Cocco, Remi Monasson, et al.. Parameters and determinants of responses to selection in antibody libraries. 2020. ⟨hal-02322339⟩

Theses1 document

  • Clément Nizak. Développement d'outils pour l'étude de la dynamique de l'appareil de Golgi en interphase et en mitose. Biophysique []. Université Paris-Diderot - Paris VII, 2003. Français. ⟨tel-00190898⟩