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Clément Campillo

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Présentation

**Research activities** **Since 2013: Assistant professor, Laboratoire d’Analyse et de Modélisation pour la Biologie et l’Environnement (LAMBE, UMR 8587, Evry), UEVE, Université Paris Saclay** Mechanics of cells probed by AFM (with J. Pelta, S. Labdi and B. Thiebot) \[18\] Oocyte mechanics \[15,16\] Biomimetic systems of the cell cortex \[17,20-23\]. **Sept. 2017: « Habilitation à Diriger les Recherches », U. Paris-Saclay** « Studying and reconstituting the mechanics of the cell cortex » **2012-2013:****Post-doc. Institut Lumière Matière, Lyon** (supervisor: J-P. Rieu) Impedance spectroscopy of cancer cells on microdevices (collaboration with R. Ferrigno, Institut des Nanotechnologies de Lyon and H. Mertani, Centre de Recherche en Cancérologie de Lyon). **June-sept. 2011: Visiting scientist, Gardel Lab, University of Chicago** Micropipette aspiration of Arp2/3 deficient cells \[14\]. **2008-2012: Post-doc. Physico-Chimie Curie (PCC), Paris** (supervisor C. Sykes) Role of membrane inclusions and actin cortex on membrane rheology by membrane nanotube extrusion (collaboration with P. Nassoy, P. Bassereau and P. Sens) \[12,13\] Membrane-cytoskeleton interactions in ciliopathy (collaboration with P. Dupuis-Williams, ESPCI) \[11\] Role of membrane tension in cell motility (collaboration with J. Plastino, PCC) \[9\] Mechanics mouse oocytes (Collaboration with M-E. Terret & M-H Verlhac, CIRB, Collège de France) \[14,16\] **2004-2007: PhD SPrAM - CEA Grenoble** (supervisors B. Pépin-Donat & A. Viallat) “Development of thermo-responsive vesicles from polyNipam systems” \[1,2,8,9,19\] Membrane mechanics of composite vesicles (collaboration with F. Brochard-Wyart, PCC) \[3,6\] Mechanics of composite vesicles (Visiting student in Institut Charles Sadron, Strasbourg, France, 6 months) \[4,5\] **Reviewer** for Nature Communications, Soft Matter, RSC Advances, ACS Omega, Biophysical Journal, New Journal of Physics, PLOS One, Physical Biology (Outstanding Reviewer award for 2018), Biomedical Physics & Engineering Express (IOP), Measurement Science and Technology (IOP) **Member** of GDR CellTiss **Grants & Fellowships** **2018** Coordinator of “High Throughput AFM”, fellowship from DIM Respore/UEVE, 82 k€ **2018 – 2021** Coordinator of “Dynactube”, ANR JCJC (young researcher fellowship, french National Research Agency), 317 k€ **2016** Partner of project AFM-UEVE, grant from region Ile de France (Sesame mi-lourd, DIM NanoK), 126 k€ (supplemented by UEVE 70 k€) **2015-2016**Teaching duty reduction (96h) from “Fonds de Rayonnement de la Recherche”, UEVE **2008-2012**Postdoctoral fellowship from french Cancer Research Association **2004-2007**PhD fellowship from International Research Training Group Grenoble-Strasbourg-Konstanz **Supervision** ***PhDs & Postdocs*** R. Lopes Dos Santos, PhD candidateon the remodelling of membrane nanotubes, from february 2019 M. Liboz, PhD candidateon the mechanics of micropatterned cancer cells, from september 2018 G. Lamour, postdoc (two years fellowship from Genopole, Evry), on the study of membrane nanotubes by AFM, from september 2016 A. Allard, PhD candidate, co-supervised by C. Sykes (PCC) and S. Labdi (LAMBE), on the effect of actin polymerization on membrane nanotubes stability \[21,23\], from january 2016 F. Valentino, PhD defended on September 27th2016, co-supervised since 2014 with C. Sykes (PCC), on actin polymerization on membrane nanotubes \[20,21\]. Now research and development engineer at Phasics (Saclay). ***Undergraduate students*** M. Liboz, Master 2 MAVINOV, U. Paris-Saclay, 4 months, 2018 C. Colne, Licence 3 Chemistry, UEVE, 3 weeks, 2018 T. Groux, Master 1 Chemistry, UEVE, 3 months, 2017 S. Brahimi, Licence 3 Chemistry, UEVE, 3 weeks, 2017 W. Hao, Licence Physique UCBL/ Wuhan University (China), 2 months, 2013 R. Pereira, Master Physical Engineering of Health, Paris 5, 2 months, 2011 G. Guiot, Ecole Polytechnique, 1st year, 3 months, 2010 L. Claverie, Ecole Polytechnique, 1st year, 3 months, 2009 F. Quémeneur, Master « Physics for life sciences», UJF, 4 months, 2005 **Communications** ***Oral presentations*** *Biomaterials Workshop (Invited talk), DIM Respore, Paris,* **2019** *“Shapes in chemistry and biology“Workshop, IPREM, Pau,* **2017** *“Active & Smart Matter Conference”, Syracuse, USA,* **2016** *Invited Seminar, LNIO, UTT, Troyes, France* **2016** *Invited Seminar, LADHYX, Ecole Polytechnique, Palaiseau, France* **2015** *Invited Seminar, LPSM, CEA Saclay* **2015** *GDR CellTiss, Mont Saint Odile,* **2014** *Invited Seminar, IMNC, Orsay* **2014** *Invited Seminar, Lenne Lab, IBDML, Luminy* **2013** *Invited Seminar, Baigl Lab, ENS Paris* **2013** *Invited Seminar, ITAV, Toulouse* **2012** *Invited Seminar, LPMCN, Lyon* **2011** *Invited Seminars, Gardel Lab, Lee Lab, University of Chicago, USA* **2011** *Invited Seminar, LADHYX, Ecole Polytechnique, Palaiseau, France* **2010** *Invited Seminar, Laboratoire de Spectrométrie Physique, Grenoble,* **2010** *GDR CellTiss, Gif-sur-Yvette,* **2009** *Softcomp Annual Meeting, Venice, Italy,* **2009** *12th Young Researchers & Life Sciences meeting, Paris,* **2009** *Biophysical Society 53rd Annual Meeting, Boston, USA,* 3 mars **2009** *Invited Seminar, SprAM, CEA-Grenoble,* **2008** *Softcomp Annual Meeting, Riva del Garda, Italy,* **2008** *Invited Seminar, Laboratoire de Spectrométrie Physique, Grenoble,***2007** *Invited Seminar, Institut Charles Sadron, Strasbourg,* **2007** *Invited Seminar, Spatz group, Max Planck Institut Stuttgart, Germany* **2007** *Congrès de la Société Française de Physique, Grenoble* **2007** *Invited Seminar, Institut Curie, Paris,* **2007** *Congrès Français de Mécanique, Troyes,***2005** *Meeting of the German Colloid Society, Aachen, Germany***2005** ***Poster presentations*** *“Membrane Biophysics of Exo-Endocytosis: from Model Systems to Cells” conference, Mandelieu* **2019** *“International Soft Matter Conference”, Grenoble* **2016** *“Physics & Biological Systems” conference, Gif-sur-Yvette* **2014** *FPGG Spring Symposium,* **2011** *Biophysical Society Conference “Actin, Cytoskeleton and Nucleus”, Singapore,* **2010** *“International Soft Matter Conference”, Granada, Spain,* **2010** *Pierre-Gilles de Gennes Winter School, Cargese,* **2008** *“Fundamental and Applied Macromolecular Science”, Strasbourg* **2007**

Publications

Mechanical Characterization of Murine Oocytes by Atomic Force Microscopy

Rose Bulteau , Lucie Barbier , Guillaume Lamour , Tristan Piolot , Elsa Labrune
Methods in Molecular Biology, 2024, Methods in Molecular Biology, 2740, pp.117-124. ⟨10.1007/978-1-0716-3557-5_7⟩
Article dans une revue hal-04476753v1

Dynamically Mapping the Topography and Stiffness of the Leading Edge of Migrating Cells Using AFM in Fast-QI Mode

Guillaume Lamour , Michel Malo , Raphaël Crépin , Juan Pelta , Sid Labdi
ACS Biomaterials Science and Engineering, 2024, ⟨10.1021/acsbiomaterials.3c01254⟩
Article dans une revue hal-04450305v1

Using Adhesive Micropatterns and AFM to Assess Cancer Cell Morphology and Mechanics

Maxime Liboz , Antoine Allard , Michel Malo , Guillaume Lamour , Gaelle Letort
ACS Applied Materials & Interfaces, 2023, ⟨10.1021/acsami.3c07785⟩
Article dans une revue hal-04201234v1
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Methods for Assessing Oocyte Quality: A Review of Literature

Yassir Lemseffer , Marie-Emilie Terret , Clément Campillo , Elsa Labrune
Biomedicines, 2022, 10 (9), pp.2184. ⟨10.3390/biomedicines10092184⟩
Article dans une revue hal-03811993v1

Studying actin-induced cell shape changes using Giant Unilamellar Vesicles and reconstituted actin networks

Rogério Lopes dos Santos , Clément Campillo
Biochemical Society Transactions, 2022, 50 (5), pp.1527-1539. ⟨10.1042/BST20220900⟩
Article dans une revue hal-03983054v1

Remodelling of membrane tubules by the actin cytoskeleton

Antoine Allard , Rogério Lopes dos Santos , Clément Campillo
Biology of the Cell, 2021, 113 (8), pp.329-343. ⟨10.1111/boc.202000148⟩
Article dans une revue hal-03641979v1
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Fluctuations of a membrane nanotube covered with an actin sleeve

A. Allard , F. Valentino , C. Sykes , T. Betz , C. Campillo
Physical Review E , 2020, 102 (5), pp.052402. ⟨10.1103/PhysRevE.102.052402⟩
Article dans une revue hal-03014749v1
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Finger-like membrane protrusions are favored by heterogeneities in the actin network

Shachar Gat , Camille Simon , Clément Campillo , Anne Bernheim-Groswasser , C. Sykes
Soft Matter, 2020, 16 (31), pp.7222-7230. ⟨10.1039/c9sm02444a⟩
Article dans une revue hal-03014841v1
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Mapping and Modeling the Nanomechanics of Bare and Protein-Coated Lipid Nanotubes

Guillaume Lamour , Antoine Allard , Juan Pelta , Sid Labdi , Martin Lenz
Physical Review X, 2020, 10 (1), pp.011031. ⟨10.1103/PhysRevX.10.011031⟩
Article dans une revue hal-02512272v1
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Actin modulates shape and mechanics of tubular membranes

A. Allard , M. Bouzid , T. Betz , C. Simon , M. Abou-Ghali
Science Advances , 2020, 6 (17), pp.eaaz3050. ⟨10.1126/sciadv.aaz3050⟩
Article dans une revue hal-02565199v1
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Artificially decreasing cortical tension generates aneuploidy in mouse oocytes

Isma Bennabi , Flora Crozet , Elvira Nikalayevich , Agathe Chaigne , Gaëlle Letort
Nature Communications, 2020, 11, ⟨10.1038/s41467-020-15470-y⟩
Article dans une revue hal-03003434v1
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Actin dynamics drive cell-like membrane deformation

Camille Simon , Remy Kusters , Valentina Caorsi , Antoine Allard , Majdouline Abou-Ghali
Nature Physics, 2019, 15 (6), pp.602-609. ⟨10.1038/s41567-019-0464-1⟩
Article dans une revue hal-02895971v1
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Interplay between membrane tension and the actin cytoskeleton determines shape changes

Camille Chane Simon , Valentina Caorsi , Clément Campillo , C. Sykes
Physical Biology, 2018, 15 (6), pp.065004. ⟨10.1088/1478-3975/aad1ab⟩
Article dans une revue hal-01980544v1
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Fluctuations of a membrane nanotube revealed by high-resolution force measurements

F. Valentino , Pierre Sens , J. Lemière , A. Allard , T. Betz
Soft Matter, 2016, 12 (47), pp.9429 - 9435. ⟨10.1039/c6sm02117d⟩
Article dans une revue hal-01411519v1

How cellular membrane properties are affected by the actin cytoskeleton

J. Lemière , F. Valentino , C. Campillo , C. Sykes
Biochimie, 2016, 130, pp.33-40. ⟨10.1016/j.biochi.2016.09.019⟩
Article dans une revue hal-02065240v1

How Synergy of Actin Assembly-Disassembly and Myosin Motors Drives Cell Shape Changes

Valentina Caorsi , Wu Sangsong , Joël Lemiere , Clément Campillo , Timo Betz
Biophysical Journal, 2016, 110 (3), pp.127a-128a. ⟨10.1016/j.bpj.2015.11.732⟩
Article dans une revue hal-02065242v1
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F-actin mechanics control spindle centring in the mouse zygote

Agathe Chaigne , Clément Campillo , Raphaël Voituriez , Nir S. Gov , C. Sykes
Nature Communications, 2016, 7, pp.10253. ⟨10.1038/ncomms10253⟩
Article dans une revue hal-01276390v1
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Elasticity, Adhesion, and Tether Extrusion on Breast Cancer Cells Provide a Signature of Their Invasive Potential

Georges Smolyakov , Bénédicte Thiebot , Clément Campillo , Sid Labdi , Childérick Séverac
ACS Applied Materials & Interfaces, 2016, 8 (41), pp.27426 - 27431. ⟨10.1021/acsami.6b07698⟩
Article dans une revue hal-01552762v1

Cell-sized liposome doublets reveal active tension build-up driven by acto-myosin dynamics

V. Caorsi , J. Lemière , Clément Campillo , M. Bussonnier , J. Manzi
Soft Matter, 2016, 12 (29), pp.6223-6231. ⟨10.1039/C6SM00856A⟩
Article dans une revue hal-02065261v1
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A narrow window of cortical tension guides asymmetric spindle positioning in the mouse oocyte

Agathe Chaigne , Clément Campillo , Nir S Gov , Raphaël Voituriez , C. Sykes
Nature Communications, 2015, 6 (1), pp.6027/1-10. ⟨10.1038/ncomms7027⟩
Article dans une revue hal-02065107v1
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Arp2/3 Inhibition Induces Amoeboid-Like Protrusions in MCF10A Epithelial Cells by Reduced Cytoskeletal-Membrane Coupling and Focal Adhesion Assembly

Yvonne Beckham , Robert J Vasquez , Jonathan Stricker , Kareem Sayegh , Clément Campillo
PLoS ONE, 2014, 9 (6), pp.e100943. ⟨10.1371/journal.pone.0100943⟩
Article dans une revue hal-02148251v1

A soft cortex is essential for asymmetric spindle positioning in mouse oocytes

Agathe Chaigne , Clément Campillo , Nir S. Gov , Raphael Voituriez , Jessica Azoury
Nature Cell Biology, 2013, 15 (8), pp.958-966. ⟨10.1038/ncb2799⟩
Article dans une revue hal-00994646v1
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α-Hemolysin membrane pore density measured on liposomes

Joel Lemiere , Karine Guevorkian , Clément Campillo , C. Sykes , Timo Betz
Soft Matter, 2013, 9, pp.3181-3187. ⟨10.1039/c3sm27812c⟩
Article dans une revue hal-01319268v1

Unexpected Membrane Dynamics Unveiled by Membrane Nanotube Extrusion

Clément Campillo , Pierre Sens , Darius Koester , Lea-Laetitia Pontani , Daniel Lévy
Biophysical Journal, 2013, 104 (6), pp.1248-1256. ⟨10.1016/j.bpj.2013.01.051⟩
Article dans une revue hal-00821323v1

Unexpected Membrane Dynamics Unveiled by Membrane Nanotube Extrusion

Clément Campillo , Pierre Sens , Darius Köster , Lea-Laetitia Pontani , Daniel Lévy
Biophysical Journal, 2013, 104 (6), pp.1248-1256. ⟨10.1016/j.bpj.2013.01.051⟩
Article dans une revue hal-02896076v1
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Mechanics of membrane-cytoskeleton attachment in Paramecium

Clément Campillo , Julie Jerber , Cathy Fisch , Maria Simoes-Betbeder , Pascale Dupuis-Williams
New Journal of Physics, 2012, 14, 125016, 9 p. ⟨10.1088/1367-2630/14/12/125016⟩
Article dans une revue hal-00814768v1

Membrane tension regulates motility by controlling lamellipodium organization

E. Batchelder , G. Hollopeter , C. Campillo , X. Mezanges , E. Jorgensen
Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (28), pp.11429-11434. ⟨10.1073/pnas.1010481108⟩
Article dans une revue hal-02896087v1
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Lipidic composite vesicles based on poly(NIPAM), chitosan or hyaluronan: behaviour under stresses

Brigitte Pepin-Donat , Clément Campillo , François Quemeneur , Marguerite Rinaudo , Carlos Marques
International Journal of Nano Dimension, 2011, 2 (1), pp.17-23. ⟨10.7508/IJND.2011.01.002⟩
Article dans une revue hal-02148160v1

Membrane tension regulates motility by controlling lamellipodium organization

Ellen L. Batchelder , Gunther Hollopeter , Clément Campillo , Xavier Mezanges , Erik M. Jorgensen
Proceedings of the National Academy of Sciences of the United States of America, 2011, 108 (28), pp.11429-11434. ⟨10.1073/pnas.1010481108⟩
Article dans une revue hal-00814820v1

Nanotubes from asymmetrically decorated vesicles

Stéphane Kremer , C. Campillo , F. Quemeneur , M. Rinaudo , B. Pepin-Donat
Soft Matter, 2011, pp.946-951
Article dans une revue hal-00577118v1

Composite gel-filled giant vesicles : Membrane homogeneity and mechanical properties

C.C. Campillo , A.P. Schroder , C.M. Marques , B. Pepin-Donat
Materials Science and Engineering: C, 2009, 29 (2), pp.393-397
Article dans une revue hal-00430699v1

Volume transition in composite poly(NIPAM)-giant unilamellar vesicles

C. C. Campillo , A. P. Schroder , C. M. Marques , B. Pepin-Donat
Soft Matter, 2008, 4 (12), pp.2486-2491
Article dans une revue hal-00359009v1

Nanotubes from gelly vesicles

Annie Viallat , Clément Campillo , Brigitte Pepin-Donat , Françoise Brochard-Wyart , Sebastien Kremer
EPL - Europhysics Letters, 2008, 82 (4), pp.48002. ⟨10.1209/0295-5075/82/48002⟩
Article dans une revue hal-00351648v1

Responsive viscoelastic giant lipid vesicles filled with a poly(N-isopropylacrylamide) artificial cytoskeleton

Clément Campillo , Brigitte Pepin-Donat , Annie Viallat
Soft Matter, 2007, 3 (11), pp.1421. ⟨10.1039/B710474J⟩
Article dans une revue hal-01957761v1
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Responsive Giant Vesicles filled with Poly(N-isopropylacrylamide) Sols or Gels

Magalie Faivre , Clément Campillo , B. Pepin-Donat , Annie Viallat
Progress in Colloid and Polymer Science, 2006, 133, pp.41. ⟨10.1007/2882_050⟩
Article dans une revue hal-00321709v1

Using the fast-QI mode to dynamically map the nanoscale topography and stiffness of lamellipodia and lamella

Guillaume Lamour , Michel Malo , Juan Pelta , S. Labdi , Clément Campillo
9th Multifrequency AFM, Jun 2023, Madrid, Spain
Communication dans un congrès hal-04181908v1

Characterizing the biomechanical alterations of human myotubes with desmin mutations using atomic force microscopy

Raphael Crepin , Pierre Joanne , Vincent Mouly , Michel Malo , Clément Campillo
European Meeting on Intermediate Filaments 2023, Jun 2023, Noordwijkerhout, Netherlands
Communication dans un congrès hal-04181938v1

Nanomechanics of lipid nanotubes

Guillaume Lamour , Antoine Allard , Juan Pelta , S. Labdi , Lenz Martin
Forum des microscopies à sonde locale, Mar 2022, Saint-Valery-Sur-Somme, France. 2022, Mar 2022, Saint-Valery-sur-Somme, France
Communication dans un congrès hal-04181371v1

Characterization of a novel subcortical actin layer regulating spindle positioning and cortical plasticity in mouse oocytes

Agathe Chaigne , Clément Campillo , Jessica Azoury , Claudia Umana-Diaz , Pierre Nassoy
Annual Meeting of the American-Society-for-Cell-Biology (ASCB), Dec 2011, Denver, United States. pp.85
Communication dans un congrès hal-00995023v1

Capsules based on lipid vesicles: potentiality for drug delivery

B. Pepin-Donat , F. Quemeneur , M. Rinaudo , C. Campillo
Nanomedicine and drug delivery, Academic Press, pp.139-153, 2012
Chapitre d'ouvrage hal-00849891v1
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Développement et étude d'objets biomimétiques stimulables : Vésicules géantes encapsulant des systèmes visco-élastiques de poly(NIPAM)

Clément Campillo
Analyse de données, Statistiques et Probabilités [physics.data-an]. Université Joseph-Fourier - Grenoble I, 2008. Français. ⟨NNT : ⟩
Thèse tel-00432264v1