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Multiphoton microscopy computationally assisted for revealing biomedical structures


Multiphoton microscopy approaches, computationally aided for improving visually the 3D image quality of thick biological samples, is implemented for delivering a new viewpoint on biological structures.

Original laser sources for multiphoton microscopy, original computational strategies for image post-processing and original biomedical samples are gathered for producing new and innovative point of view on 3D biomedical structures. Muscle structure is the first tested and the axial organisation has been highlighted for the first time thus reaveling tubular organization.


Journal articles26 documents

  • Thomas Hortholary, Claire Carrion, Emilie Chouzenoux, Jean-Christophe Pesquet, Claire Lefort. Multiplex‐multiphoton microscopy and computational strategy for biomedical imaging. Microscopy Research and Technique, Wiley, In press, ⟨10.1002/jemt.23712⟩. ⟨hal-03126396⟩
  • Emilie Chouzenoux, Tim Tsz-Kit Lau, Claire Lefort, Jean-Christophe Pesquet. Optimal Multivariate Gaussian Fitting with Applications to PSF Modeling in Two-Photon Microscopy Imaging. Journal of Mathematical Imaging and Vision, Springer Verlag, 2019, 61 (7), pp.1037-1050. ⟨10.1007/s10851-019-00884-1⟩. ⟨hal-01985663⟩
  • David Moreau, Claire Lefort, Jolien Pas, Sylvia Bardet, Philippe Lévêque, et al.. Infrared neural stimulation induces intracellular Ca 2+ release mediated by phospholipase C. Journal of Biophotonics, Wiley, 2017, ⟨10.1002/jbio.201700020⟩. ⟨hal-01656507⟩
  • Claire Lefort. A review of biomedical multiphoton microscopy and its laser sources. Journal of Physics D: Applied Physics, IOP Publishing, 2017, 50 (42), pp.423001. ⟨10.1088/1361-6463/aa8050⟩. ⟨hal-02481431⟩
  • Claire Lefort. A review of biomedical multiphoton microscopy and its laser sources. Journal of Physics D: Applied Physics, IOP Publishing, 2017, ⟨10.1088/1361-6463/aa8050⟩. ⟨hal-01571406⟩
  • Farid El Bassri, Claire Lefort, Erwan Capitaine, Christophe Louot, Dominique Pagnoux, et al.. Nanosecond Coherent Anti-Stokes Raman Scattering for Particle Size Characterization. Proceedings of SPIE, the International Society for Optical Engineering, SPIE, The International Society for Optical Engineering, 2016, Optical Biopsy XIV: Toward Real-Time Spectroscopic Imaging and Diagnosis, 9703, pp.970314. ⟨10.1117/12.2212501⟩. ⟨hal-01301658⟩
  • Christophe Louot, Erwan Capitaine, Badr Mohamed Ibrahim Shalaby, Katarzyna Krupa, Alessandro Tonello, et al.. All-normal dispersion supercontinuum generation in the near-infrared by Raman conversion in standard optical fiber. SPIE Proceedings Series, 2016, Nonlinear Frequency Generation and Conversion: Materials, Devices, and Applications XV (proc. 9731), pp.97310M. ⟨10.1117/12.2208580⟩. ⟨hal-01288127⟩
  • Claire Lefort, Rodney O'Connor, Véronique Blanquet, L. Magnol, H. Kanoh, et al.. Multicolor multiphoton microscopy based on a nanosecond supercontinuum laser source. Journal of Biophotonics, Wiley, 2016, 9 (7), pp.709-714. ⟨10.1002/jbio.201500283⟩. ⟨hal-01321985⟩
  • Erwan Capitaine, Christophe Louot, Nawell Ould-Moussa, Claire Lefort, J.F. Kaneyasu, et al.. M-CARS and EFISHG study of the influence of a static electric field on a non-polar molecule. Proceedings of SPIE, the International Society for Optical Engineering, SPIE, The International Society for Optical Engineering, 2016, Multiphoton Microscopy in the Biomedical Sciences XVI, 9712, pp.97120M. ⟨10.1117/12.2208847⟩. ⟨hal-01293930⟩
  • Erwan Capitaine, Nawell Ould-Moussa, Christophe Louot, Claire Lefort, Dominique Pagnoux, et al.. Coherent anti-Stokes Raman scattering under electric field stimulation. Physical Review B: Condensed Matter and Materials Physics (1998-2015), American Physical Society, 2016, 94 (24), pp.245136. ⟨10.1103/PhysRevB.94.245136⟩. ⟨hal-01508681⟩
  • A. Ibrahim, Fanny Poulon, R. Habert, Claire Lefort, Alexandre Kudlinski, et al.. Characterization of fiber ultrashort pulse delivery for nonlinear endomicroscopy. Optics Express, Optical Society of America - OSA Publishing, 2016, 24 (12), pp.12515-12523. ⟨10.1364/OE.24.012515⟩. ⟨hal-01656576⟩
  • Philippe Leproux, Vincent Couderc, Claire Lefort. Compact supercontinuum sources for biomedical applications. SPIE Proceedings Photonics West, 2015, pp.1. ⟨hal-01127220⟩
  • David Moreau, Claire Lefort, Burke Ryan, Philippe Lévêque, Rodney O'Connor. Rhodamine B as an optical thermometer in cells focally exposed to infrared laser light or nanosecond pulsed electric fields. Biomedical optics express, Optical Society of America - OSA Publishing, 2015. ⟨hal-01218493⟩
  • Claire Lefort, David Moreau, Philippe Lévêque, Rodney O'Connor. Optical measurement of temperature in tissue culture surfaces under infrared laser light excitation at 800nm using a fluorescent dye. Photoptics, 2015, pp.1. ⟨hal-01127229⟩
  • Hussein Hamzeh, Claire Lefort, Frederic Pain, Darine Abi Haidar. Optimization and characterization of a nonlinear excitation and collection through a Gradient-index lens for high resolution nonlinear endomicroscopy of brain tissues. Chinese Optics Letters, Optical Society of America (imprimé) / OSA publishing (en ligne), 2015, pp.808-811. ⟨hal-01127214⟩
  • Claire Lefort, David Sevrain, Hussein Hamzeh, Philippe Leproux, Frederic Pain, et al.. Design of an optimized distal optic for non linear endomicroscopy. Photoptics, 2015, pp.1. ⟨hal-01127235⟩
  • Hussein Hamzeh, Claire Lefort, Frederic Pain, Darine Abi Haidar. Optimization and characterization of nonlinear excitation and collection through a gradient-index lens for high-resolution nonlinear endomicroscopy. Optics Letters, Optical Society of America - OSA Publishing, 2015. ⟨hal-01218491⟩
  • Tayssir Ibrahim, Daniel Daugeron, Serge Battu, Issa-Ali Moustafa, Claire Lefort, et al.. Dye-free determination of the focalization position for the hollow fiber flow field flow fractionation (HF5) of proteins. Analytical and Bioanalytical Chemistry, Springer Verlag, 2014. ⟨hal-01218498⟩
  • Claire Lefort, Méri Kalashyan, Guillaume Ducourthial, Tigran Mansuryan, Rodney O'Connor, et al.. Sub-30-fs pulse compression and pulse shaping at the output of a 2-m-long optical fiber in the near-infrared range. Journal of the Optical Society of America B, Optical Society of America, 2014, 31 (10), pp.2317. ⟨hal-01063850⟩
  • Tayssir Ibrahim, Daniel Daugeron, Serge Battu, Mustapha Belaissaoui, Claire Lefort, et al.. Flow and stop flow elution of proteins in hollow fiber flow field flow fractionation (HF5) systems. Analytical and Bioanalytical Chemistry, Springer Verlag, 2014, pp.1-4. ⟨hal-01127205⟩
  • Claire Lefort, Hussein Hamzeh, Frédéric Louradour, Frederic Pain, Darine Abi Haidar. Characterization, comparison, and choice of a commercial double-clad fiber for nonlinear endomicroscopy. JBO, 2014, 19 (7), pp.076005. ⟨hal-01063851⟩
  • Donald A. Peyrot, Claire Lefort, Marie Steffenhagen, Tigran Mansuryan, Guillaume Ducourthial, et al.. Development of a nonlinear fiber-optic spectrometer for human lung tissue exploration. Biomedical optics express, Optical Society of America - OSA Publishing, 2012, 3 (5), pp.840-853. ⟨10.1364/BOE.3.000840⟩. ⟨hal-00736075⟩
  • Donald A. Peyrot, Claire Lefort, Tigran Mansuryan, Sergei Kruglik, Nicolas Sandeau, et al.. Développement d'un endomicroscope non linéaire pour l'observation in vivo in situ de la matrice extracellulaire des tissus pulmonaires. Innovation and Research in BioMedical engineering, Elsevier Masson, 2012, 33 (2), pp.67-72. ⟨10.1016/j.irbm.2012.01.011⟩. ⟨hal-00941229⟩
  • Donald A Peyrot, Claire Lefort, Marie Steffenhagen, Tigran Mansuryan, Guillaume Ducourthial, et al.. Development of a nonlinear fiber-optic spectrometer for human lung tissue exploration.. Biomedical optics express, Optical Society of America - OSA Publishing, 2012, 3 (5), pp.840-53. ⟨hal-01153711⟩
  • Méri Kalashyan, Claire Lefort, Lluis Martinez-Leon, Tigran Mansuryan, Levon Mouradian, et al.. Ultrashort pulse fiber delivery with optimized dispersion control by reflection grisms at 800 nm. Optics Express, Optical Society of America - OSA Publishing, 2012, 23 (20), pp.25624-25635. ⟨hal-00917063⟩
  • Claire Lefort, Tigran Mansuryan, Frédéric Louradour, Alain Barthélémy. Pulse compression and fiber delivery of 45 fs Fourier transform limited pulses at 830 nm. Optics Letters, Optical Society of America - OSA Publishing, 2011, 36 (2), pp.292-294. ⟨10.1364/OL.36.000292⟩. ⟨hal-00688265⟩

Conference papers38 documents

  • Claire Lefort, Emilie Chouzenoux, Laetitia Magnol, Henri Massias, Jean-Christophe Pesquet. Multi-parametric 3D-point-spread function estimation in deep multiphoton microscopy with an original computational strategy dedicated to the reconstruction of muscle images. SPIE Photonics Europe, Apr 2020, Online Only, United States. ⟨10.1117/12.2554742⟩. ⟨hal-02566520⟩
  • Ali Abjaghou, Claire Carrion, Laetitia Magnol, Claire Lefort. Resolution and computational strategy in wideband multiphoton microscopy illustrated with muscle imaging. Optics and Photonics for Advanced Dimensional Metrology, Apr 2020, Online Only, France. pp.34, ⟨10.1117/12.2555048⟩. ⟨hal-02566517⟩
  • Thomas Hortholary, Claire Carrion, Claire Lefort. Advanced biomedical multiphoton fluorescence microscopy with a large band excitation system. Biomedical Spectroscopy, Microscopy, and Imaging, Apr 2020, Online Only, France. pp.33, ⟨10.1117/12.2552926⟩. ⟨hal-02991372⟩
  • Claire Lefort, Emilie Chouzenoux, Jean-Christophe Pesquet. PLUMEE 2019 : Reconstruction numérique d'image en microscopie multiphotonique. PLUMEE 2019 - 6ème Colloque francophone PLUridisciplinaire sur les Matériaux, l’Environnement et l’Electronique, Apr 2019, Limoges, France. ⟨hal-02426765⟩
  • Claire Lefort, Laetitia Magnol, Véronique Blanquet, Philippe Lévêque. Endogenous two- and three-photon fluorescence of a biological substance with a picosecond ultrawide band laser source. Optics, Photonics and Digital Technologies for Imaging Applications, Apr 2018, Strasbourg, France. pp.58, ⟨10.1117/12.2306681⟩. ⟨hal-02465516⟩
  • Tim Tsz-Kit, Emilie Chouzenoux, Claire Lefort, Jean-Christophe Pesquet. Optimal Multivariate Gaussian Fitting for PSF Modeling in Two-Photon Microscopy. 15 th IEEE International Symposium on Biomedical Imaging (ISBI 2018), Apr 2018, Washington DC, United States. ⟨10.1109/ISBI.2018.8363621⟩. ⟨hal-01803413⟩
  • David Moreau, Claire Lefort, Sylvia Bardet, Philippe Lévêque, Rodney O'Connor. Mesures de température dans des systèmes biologiques sous excitation infrarouge. JNOG 2017, Jul 2017, Limoges, France. ⟨hal-01583041⟩
  • David Moreau, Claire Lefort, Sylvia Bardet, Rodney O'Connor. Studying the mechanism of neurostimulation by infrared laser light using GCaMP6s and Rhodamine B imaging. Conference on Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation, Feb 2017, San Francisco, CA, United States. ⟨hal-01676332⟩
  • Claire Lefort, Rodney O'Connor, Sylvia Bardet, Véronique Blanquet, Laetitia Magnol, et al.. IMAGERIE MULTIPHOTONIQUE DE TISSUS MUSCULAIRES PAR SOURCE ULTRALARGE SPECTRE. JNOG 2017 Journées Nationales d’Optique Guidée 2017, Sep 2017, Limoges, France. ⟨hal-01583036⟩
  • David Moreau, Claire Lefort, Sylvia Bardet, Rodney O'Connor. Studying the mechanism of neurostimulation by infrared laser light using GCaMP6s and Rhodamine B imaging. SPIE photonics west BIOS, 2016, San francisco, United States. ⟨hal-02115473⟩
  • Erwan Capitaine, Christophe Louot, Claire Lefort, Dominique Pagnoux, Vincent Couderc, et al.. Electric Field Mapping and Dosimetry by means of Spontaneous and Coherent Raman Microspectroscopy. Photonic West conference, Feb 2016, San Francisco, United States. paper 9712-19. ⟨hal-01288120⟩
  • Farid El Bassri, Claire Lefort, Erwan Capitaine, Christophe Louot, Dominique Pagnoux, et al.. Nanosecond Coherent Anti-Stokes Raman Scattering for Particle Size Characterization. Photonic West, Feb 2016, San Francisco, United States. pp.9703-38. ⟨hal-01277749⟩
  • Jean-René Duclere, Philippe Leproux, Gaëlle Delaizir, Alessandro Tonello, Katarzyna Krupa, et al.. IMAGERIE NON LINEAIRE A BASE DE SOURCES LASERS MINIATURES. ICOPA 4, Dec 2016, Pessac, France. ⟨hal-01871618⟩
  • Christophe Louot, Erwan Capitaine, Badr Mohamed Ibrahim Shalaby, Katarzyna Krupa, Alessandro Tonello, et al.. All-normal dispersion supercontinuum generation in the near-infrared by Raman conversion in standard optical fiber. Photonic West, Feb 2016, San Francisco, United States. pp.9731-20. ⟨hal-01277746⟩
  • Claire Lefort, Rodney P. O'Connor, Véronique Blanquet, Fabienne Baraige, Vincent Tombelaine, et al.. Multiphoton imaging with a nanosecond supercontinuum source. Conference on Multiphoton Microscopy in the Biomedical Sciences XVI, Feb 2016, San Francisco, United States. ⟨10.1117/12.2208134⟩. ⟨hal-01382942⟩
  • Philippe Leproux, Vincent Couderc, Erwan Capitaine, Nawell Ould-Moussa, Christophe Louot, et al.. Multimodal imaging under supercontinuum illumination and electric field stimulation. 4eme symposium du LAPHIA, LAPHIA Bordeaux, Sep 2016, Bordeaux, France. ⟨hal-01391546⟩
  • David Moreau, Claire Lefort, Philippe Lévêque, Rodney O'Connor. Optical measurement of temperature in biological cells under infrared laser light exposure (λ=800 nm). European Conferences on Biomedical Optics, 22nd International Congress on Photonics in Europe, SPIE, Jun 2015, Munich, Germany. ⟨10.1117/12.2183805⟩. ⟨hal-01336791⟩
  • Christophe Louot, Erwan Capitaine, Dominique Pagnoux, Philippe Leproux, Claire Lefort, et al.. Conversion de fréquence dans les fibres optiques : application à la microscopie non linéaire sous influence électrique. . ENOVA, 4eme congrès des applications des Fibres Optiques, Sep 2015, Paris, France. ⟨hal-01288114⟩
  • Claire Lefort, Hussein Hamzeh, Liu Wei, Frederic Pain, Darine Abi Haidar. Focus on the Distal Assembly of a Nonlinear Endomicroscope: A Gradient Index Lens Coupled with a Piezoelectric Scanner for Fluorescence Lifetime Measurements. 4th International Conference on Photonics, Optics and Laser Technology (PHOTOOPTICS), Mar 2015, Berlin, Germany. pp.19-33. ⟨hal-01656543⟩
  • Philippe Leproux, Vincent Couderc, Claire Lefort. The promise of compact supercontinuum for biomedicine. Strategies in biophotonics 2014, Sep 2014, Boston, United States. ⟨hal-01063858⟩
  • Claire Lefort, Hussein Hamzeh, Darine Abi Haidar. Toward nonlinear multimodal endomicroscopy combining large field of view and high resolution imagining. 2nd Workshop on complex materials, Jun 2014, Paris, France. ⟨hal-01063857⟩
  • Claire Lefort, Hussein Hamzeh, Frédéric Louradour, Frederic Pain, Darine Abi Haidar. Caractérisation, comparaison et choix d'une fibre endomicroscopique à double gaine pour l'endomicroscopie non linéaire. JIONC, Mar 2014, Paris, France. ⟨hal-01063860⟩
  • Claire Lefort, Hussein Hamzeh, Frédéric Louradour, Frederic Pain, Darine Abi Haidar. Vers un endomicroscope non linéaire multimodal : caractérisation, comparaison et choix d'une fibre optique endomicroscopique à double gaine. Optodiag, May 2014, Paris, France. ⟨hal-01063859⟩
  • Guillaume Ducourthial, Claire Lefort, Donald A. Peyrot, Tigran Mansuryan, Sergei Kruglik, et al.. Label free multiphoton imaging of human pulmonary tissues through two-meter-long microstructured fiber and multicore image-guide. BIOS - Proc. SPIE 8575, Endoscopic Microscopy VIII, 85750H, Feb 2013, San Fransisco, United States. pp.85750H, ⟨10.1117/12.2003118⟩. ⟨hal-00914654⟩
  • Hussein Hamzeh, Claire Lefort, Jamal Charara, Frédéric Louradour, Gael Latour, et al.. Optical designs for multimodal nonlinear microendoscope for optical biopsy. Advances in Biomedical Engineering (ICABME), 2013, Sep 2013, Tripoli, Lebanon. pp.155-158, ⟨10.1109/ICABME.2013.6648871⟩. ⟨hal-00914648⟩
  • Hussein Hamzeh, Claire Lefort, Frederic Pain, Darine Abi Haidar. Optimization of Nonlinear Multimodal Microendoscopy for Optical Biopsy. IONS 2013, Mar 2013, Poland. ⟨hal-00918826⟩
  • Hussein Hamzeh, Claire Lefort, Frédéric Louradour, Gaël Latour, Frederic Pain, et al.. Optimization of Nonlinear Multimodal Microendoscopy Based on DCF and GRIN Lenses for Optical Biopsy. Frontiers in Optics, Oct 2013, Floride, United States. pp.978-1-55752-987-9. ⟨hal-00909779⟩
  • Donald Peyrot, Darine Abi Haidar, Claire Lefort, Nicolas Sandeau, Tigran Mansuryan, et al.. Toward non-linear endomicroscopy for exploration of the pulmonary airways : preliminary spectroscopic study of human lung tissue using monomode fiber. Endoscopic Microscopy VI, Nonlinear Endomicroscopy, Jan 2011, San franscisco - Californie, United States. pp.n°789309, ⟨10.1117/12.875440⟩. ⟨hal-00688280⟩
  • Claire Lefort, Méri Kalashyan, Tigran Mansuryan, Levon Mouradian, Alain Barthélémy, et al.. Déport par fibre optique d'impulsions femtosecondes de duree record egale a 17,8 femtosecondes a 820 nm. JNOG 2011, Jul 2011, Marseille, France. pp.Inconnu. ⟨hal-00689095⟩
  • Claire Lefort, Méri Kalashyan, Tigran Mansuryan, Frédéric Louradour, Alain Barthélémy. Transport d'impulsions lumineuses ultra-brèves. Deuxième colloque francophone PLUridisciplinaire sur les Matériaux, l'Environnement et l'Electronique -PLUMEE 2011, May 2011, Limoges, France. ⟨hal-00689319⟩
  • Jérôme Desroches, Claire Lefort, Guillaume Ducourthial, Tigran Mansuryan, Julien Brevier, et al.. Imagerie polarimétrique par fibre optique. Workshops 'Journée Électromagnétisme, Polarisation et Optique Statistique' - Institut Fresnel, Nov 2011, Marseille, France. ⟨hal-00688080⟩
  • Claire Lefort, Méri Kalashyan, Donald Peyrot, Tigran Mansuryan, Levon Mouradian, et al.. Pulse compression and fiber delivery of Sub-30 fs nanojoule pulses at 830 nm. Conference on Lasers and Electro-Optics US, CWR4, Ultrafast Pulse Generation II session, May 2011, Baltimore - Mariland, United States. pp.1-2. ⟨hal-00688356⟩
  • Claire Lefort, Méri Kalashyan, Tigran Mansuryan, Donald Peyrot, Levon Mouradian, et al.. Dispersion compensation with a grism-based dispersive line for fiber delivery of sub-30 fs pulses at 830 nm. Conference on Lasers and Electro-Optics Europe, May 2011, Munich, Germany. pp.1-1, ⟨10.1109/CLEOE.2011.5942662⟩. ⟨hal-00688391⟩
  • Claire Lefort, Méri Kalashyan, Tigran Mansuryan, Levon Mouradian, Frédéric Louradour, et al.. Fiber delivery of sub-30 fs pulses at 830 nm using a grism-based dispersive line. IONS-9 OSA Conference - 11th of may, Apr 2011, Salamanca, Spain. OP8 - Laser development II. ⟨hal-00688420⟩
  • Claire Lefort, Méri Kalashyan, Tigran Mansuryan, Frédéric Louradour. Transport par fibre optique de longueur métrique d'impulsions lumineuses de durée inférieure à 20 fs et d'énergie supérieure à 1 nJ. Journées des Phénomènes Ultrarapides, Oct 2011, Rouen, France. ⟨hal-00689011⟩
  • Claire Lefort, Guillaume Ducourthial, Tigran Mansuryan, Mireille Verdier, Alain Barthélémy, et al.. Imagerie non linéaire par fibre optique. Workshop XLIM, Jul 2011, Limoges, France. ⟨hal-00689297⟩
  • Frédéric Louradour, Claire Lefort, Jérôme Desroches, Tigran Mansuryan, Dominique Pagnoux, et al.. Résultats récents concernant l'imagerie par fibres optiques. COLOQ12, Jul 2011, Marseille, France. ⟨hal-00687645⟩
  • Claire Lefort, Tigran Mansuryan, Frédéric Louradour, Alain Barthélémy. Acheminement d'impulsions femtosecondes par fibre optique. JNOG 2010, Oct 2010, Besançon, France. ⟨hal-00909772⟩

Poster communications1 document

  • David Moreau, Claire Lefort, Philippe Lévêque, Rodney O'Connor. Optical measurement of temperature modification induced by infrared light exposure. 3rd EOS Conference on Optofluidics, Jun 2015, Munich, Germany. 3rd EOS Conference on Optofluidics. ⟨hal-01336790⟩

Books1 document

  • Claire Lefort. Transport d'impulsions femtosecondes par fibres optiques pour des applications à la biophotonique. Université de Limoges, pp.154, 2012. ⟨hal-00934180⟩

Book sections1 document

  • Claire Lefort, Hussein Hamzeh, Liu Wei, Frédéric Pain, Darine Abi Haidar. Focus on the Distal Assembly of a Nonlinear Endomicroscope: A Gradient Index Lens Coupled with a Piezoelectric Scanner for Fluorescence Lifetime Measurements. 4th International Conference on Photonics, Optics and Laser Technology (PHOTOOPTICS), Berlin, GERMANY, 2015, pp.19-33, 2016, ⟨10.1007/978-3-319-30137-2_2⟩. ⟨in2p3-02025157⟩

Directions of work or proceedings1 document

  • David Moreau, Claire Lefort, Sylvia Bardet, Rodney O'Connor. Studying the mechanism of neurostimulation by infrared laser light using GCaMP6s and Rhodamine B imaging. SPIE BiOS, 2016, San Francisco, Proceedings of SPIE, the International Society for Optical Engineering, 9690 (96901M), 2016, Clinical and Translational Neurophotonics; Neural Imaging and Sensing; and Optogenetics and Optical Manipulation;, ⟨10.1117/12.2211355⟩. ⟨hal-02469376⟩