Nombre de documents

137

Bruno Le Pioufle


Bruno LE PIOUFLE,  Professor at the Ecole Normale Supérieure of Cachan

E.N.S. de Cachan, 61 av du Pdt Wilson, 94235 Cachan, France,

Phone: 001 33 1 47407736, Fax: 001 33 1 47402199, email: bruno.le-pioufle@ens-cachan.fr


 

 EDUCATION:

1997:                   Accreditation to supervise research , University of PARIS XI

1991:                   French Ph.D., University of  PARIS XI

 

PROFESSIONAL EXPERIENCE:

since 2007:                  Professor at ENS Cachan, IFR d’ALEMBERT, SATIE, BIOMIS group
2005-2007:                  Director of LIMMS/CNRS IIS,UMI 2820, University of Tokyo, group of S. Takeuchi
1999:                           Professor à l’ENS de Cachan, Creation and Direction of BIOMIS group(1999-2005)
1997-1999:                  CNRS researcher at LIMMS, University of Tokyo, IIS, group of H. Fujita
1992-1997:                  Associate professor ar ENS  Cachan

 

MAIN RESEARCH ACTIVITIES

B. Le Pioufle have been working since 1997 on the conception of bio-medical purpose microsystems. His main scientific concerns are 1) the conception of microfluidic biochips for the monitoring of membrane proteins and ion channels and 2) cell biochips. He spent 4 years at University of Tokyo, in the groups of H. Fujita, then in the group of S. Takeuchi. He was Director of LIMMS (International laboratory between CNRS and University of Tokyo). He  worked in collaboration with S. Takeuchi on the conception of parallelized biochips for the screening on transmembrane molecules. He is also working on biochips for the cell analysis and treatment. He is working on cell handling on a chip using dielectrophoresis, cell electroporation on a chip, microfluidics, surface functionalization of biochips. The main projects on which he has been working are 1) the study and conception of highly parallelized cell biochips for analysis (high throughput screening) or treatment (gene transfection on a chip)  2) the study and conception of biochips for ex-vivo (on a chip) analysis of physiological models 3) the design of biochips designed for the delivery of high amplitude  electrical nanosecond pulses for the treatment of cells.

 

 


Article dans une revue57 documents

  • Abdelhatif El Fellahi, Thierry Bore, Sakina Bensalem, Pierre Bodénès, Filipa Lopes, et al.. Monitoring of Microalgae Lipid Accumulation Using RF Open Ended Biosensor. IEEE Sensors Letters, IEEE, 2017, 1 (4), pp. 4500303. 〈http://ieeexplore.ieee.org/abstract/document/7934458/〉. 〈10.1109/LSENS.2017.2709082〉. 〈hal-01587262〉
  • Julien Picot, Papa Alioune Ndour, Sophie D. Lefevre, Wassim El Nemer, Harvey Tawfik, et al.. A biomimetic microfluidic chip to study the circulation and mechanical retention of red blood cells in the spleen. American Journal of Hematology, Wiley, 2015, 〈10.1002/ajh.23941〉. 〈hal-01113050〉
  • Julien Duez, John P. Holleran, Papa Alioune Ndour, Sasdekumar Loganathan, Pascal Amireault, et al.. Splenic retention of Plasmodium falciparum gametocytes to block the transmission of malaria. Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 2015, pp.AAC.05030-14. 〈10.1128/AAC.05030-14〉. 〈hal-01158996〉
  • Eric Perrin, Olivier Français, Bruno Le Pioufle, Bertrand David. Reproducing topography and roughness of osteoconductive biomaterials in a microfluidic device. Computer Methods in Biomechanics and Biomedical Engineering: Imaging & Visualization, Taylor & Francis, 2015, 18, pp.2026-2027. 〈http://www.tandfonline.com/doi/abs/10.1080/10255842.2015.1069567?journalCode=gcmb20〉. 〈10.1080/10255842.2015.1069567〉. 〈hal-01222451〉
  • Rémi Sieskind, Claudia Trainito, Olivier Français, Bruno Le Pioufle. Microsystème dédié à l’étude de la polarisation diélectrique de microparticules dans le cadre de formation master recherche : application au micropositionnement 3D de cellules par force de diélectrophorèse. Journal sur l'enseignement des sciences et technologies de l'information et des systèmes, EDP Sciences, 2015, 14, http://dx.doi.org/10.1051/j3ea/2015007. 〈10.1051/j3ea/2015007〉. 〈hal-01122450〉
  • Emilie Bisceglia, Myriam Cubizolles, Claudia Irene Trainito, Jean Berthier, Catherine Pudda, et al.. A generic and label free method based on dielectrophoresis for the continuous separation of microorganism from whole blood samples. Sensors and Actuators B: Chemical, Elsevier, 2015, 212, pp.335-343. 〈10.1016/j.snb.2015.02.024〉. 〈hal-01121775〉
  • Claudia Irene Trainito, Olivier Français, Bruno Le Pioufle. Monitoring the permeabilization of a single cell in a microfluidic device, through the estimation of its dielectric properties based on combined dielectrophoresis and electrorotation in-situ experiments. ELECTROPHORESIS, Wiley-VCH Verlag, 2015, 〈10.1002/elps.201400482〉. 〈hal-01116751〉
  • Feriel Sihem Hamdi, M. Woytasik, Magdalèna Couty, Olivier Francais, Bruno Le Pioufle, et al.. Low Temperature Irreversible Poly(DiMethyl) Siloxane Packaging of Silanized SU8 Microchannels: Characterization and Lab-on-Chip Application. Journal of Microelectromechanical Systems, Institute of Electrical and Electronics Engineers, 2014, pp.ISSN 1057-7157. 〈10.1109/JMEMS.2014.2331454〉. 〈hal-01063867〉
  • Feriel Sihem Hamdi, Olivier Francais, Elisabeth Dufour-Gergam, Bruno Le Pioufle. How medium osmolarity influences dielectrophoretically assisted on-chip electrofusion. Bioelectrochemistry, Elsevier, 2014, pp.42-46. 〈10.1016/j.bioelechem.2014.05.004〉. 〈hal-01026162〉
  • Martinus H. V. Werts, Florent Allix, Olivier Francais, Céline Frochot, Laurent Griscom, et al.. Manipulation and optical detection of colloidal functional plasmonic nanostructures in microfluidic systems. IEEE Journal of Selected Topics in Quantum Electronics, Institute of Electrical and Electronics Engineers, 2014, 20, pp.6900613. 〈10.1109/JSTQE.2013.2284549〉. 〈hal-00961328〉
  • Feriel Sihem Hamdi, Olivier Français, Frédéric Subra, Wei Wang, Marion Woytasik, et al.. Study of the transmembrane potential distribution of cell pairs in a microfluidic device using polymer obstacles to initiate electrofusion. European Physical Journal: Applied Physics, EDP Sciences, 2013, 62 (01), pp.11202-11212. 〈10.1051/epjap/2013120392〉. 〈hal-00812130〉
  • Olivier Francais, Bruno Le Pioufle. La mesure de la bio-impédance d'un végétal comme moyen d'introduire le domaine de la biologie dans une formation E.E.A.. Journal sur l'enseignement des sciences et technologies de l'information et des systèmes, EDP Sciences, 2013, 12, 2013, pp.0018. 〈10.1051/j3ea/2013018〉. 〈hal-00934120〉
  • Emilie Bisceglia, Myriam Cubizolles, Frederic Mallard, Francoise Vinet, Olivier Francais, et al.. Micro-organism extraction from biological samples using DEP forces enhanced by osmotic shock. Lab on a Chip, Royal Society of Chemistry, 2013, 13 (5), pp.901-909. 〈10.1039/c2lc41128h〉. 〈hal-00785335〉
  • Feriel S Hamdi, Olivier Français, Frederic Subra, Elisabeth Dufour-Gergam, Bruno Le Pioufle. Microarray of non-connected gold pads used as high density electric traps for parallelized pairing and fusion of cells. Biomicrofluidics, 2013, 7, pp.044101. 〈10.1063/1.4813062〉. 〈hal-00861239〉
  • Wei Wang, Laura Monlezun, Martin Picard, Philippe Benas, Olivier Français, et al.. Activity Monitoring of Functional OprM Using a Biomimetic Microfluidic Device. Analyst, Royal Society of Chemistry, 2012, 137 (4), pp.847-852. 〈10.1039/c2an16007b〉. 〈hal-00739216〉
  • Claire Dalmay, M. A. De Menorval, O. Francais, L. M. Mir, Bruno Le Pioufle. A microfluidic device with removable packaging for the real time visualisation of intracellular effects of nanosecond electrical pulses on adherent cells. Lab on a Chip, Royal Society of Chemistry, 2012, 12 (22), pp.4709-15. 〈10.1039/c2lc40857k〉. 〈hal-00745203〉
  • Guillaume Mottet, Bruno Le Pioufle, Lluis M. Mir. High-resolution analyses of cell fusion dynamics in a biochip. ELECTROPHORESIS, Wiley-VCH Verlag, 2012, 33 (16), pp.2508-2515. 〈10.1002/elps.201200112〉. 〈hal-00738706〉
  • M. Chabanon, H. Duval, O. Francais, Bruno Le Pioufle, E. Perrin, et al.. Discrete model combined with mimetic microfluidic chips to study cell growth in porous scaffold under flow conditions.. Computer Methods in Biomechanics and Biomedical Engineering, London : Informa Healthcare, 2012, 15 (1), pp.25-26. 〈10.1080/10255842.2012.713699〉. 〈hal-00751801〉
  • Claire Dalmay, Julien Villemejane, Vanessa Joubert, Aude Silve, Delia Arnaud-Cormos, et al.. A microfluidic biochip for the nanoporation of living cells. Biosensors and Bioelectronics, Elsevier, 2011, 26 (12), pp.4649-4655. 〈10.1016/j.bios.2011.03.020〉. 〈hal-00674584〉
  • Claire Dalmay, Julien Villemejane, Vanessa Joubert, Olivier Francais, Lluis M. Mir, et al.. Design and realization of a microfluidic device devoted to the application of ultra-short pulses of electrical field to living cells. Sensors and Actuators B: Chemical, Elsevier, 2011, 160 (1), pp.1573-1580. 〈10.1016/j.snb.2011.09.009〉. 〈hal-00738711〉
  • Marie Breton, Gaëlle Prével, Jean-Frédéric Audibert, Robert Pansu, Patrick Tauc, et al.. Solvatochromic dissociation of non-covalent fluorescent organic nanoparticles upon cell internalization.. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2011, epub ahead of print. 〈10.1039/c1cp20877b〉. 〈hal-00604305〉
  • Marie Breton, Gaelle Prevel, Jean-Frederic Audibert, Robert Pansu, Patrick Tauc, et al.. Solvatochromic dissociation of non-covalent fluorescent organic nanoparticles upon cell internalization. Physical Chemistry Chemical Physics, Royal Society of Chemistry, 2011, 13 (29), pp.13268-13276. 〈10.1039/c1cp20877b〉. 〈hal-00739217〉
  • Guillaume Mottet, Julien Villemejane, Luis Mir, Bruno Le Pioufle. A technique to design complex 3D lab on a chip involving multilayered fluidics, embedded thick electrodes and hard packaging. Application to dielectrophoresis and electroporation of cells.. Journal of Micromechanics and Microengineering, IOP Publishing, 2010, 20, pp.047001. 〈10.1088/0960-1317/20/4/047001〉. 〈hal-00592400〉
  • Hiroaki Suzuki, Bruno Le Pioufle, Shoji Takeuchi. Ninety-six-well planar lipid bilayer chip for ion channel recording Fabricated by hybrid stereolithography. Biomedical Microdevices, Springer Verlag, 2009, 11 (1), pp.17-22. 〈10.1007/s10544-008-9205-4〉. 〈hal-00599926〉
  • Bassam Hajj, Sophie De Reguardati, Loic Hugonin, Bruno Le Pioufle, Toshihisa Osaki, et al.. Electro-Optical Imaging Microscopy of Dye-Doped Artificial Lipidic Membranes. Biophysical Journal, Biophysical Society, 2009, 97 (11), pp.2913-2921. 〈10.1016/j.bpj.2009.08.055〉. 〈hal-00599955〉
  • Toshihisa Osaki, Hiroaki Suzuki, Bruno Le Pioufle, Shoji Takeuchi. Multichannel Simultaneous Measurements of Single-Molecule Translocation in α-Hemolysin Nanopore Array. Analytical Chemistry, American Chemical Society, 2009, 81 (24), pp.9866-9870. 〈10.1021/ac901732z〉. 〈hal-00599956〉
  • Peter Low, Bruno Le Pioufle, Beomjoon Kim, Christian Bergaud. Assembly of CdSe/ZnS nanocrystals on microwires and nanowires for temperature sensing. Sensors and Actuators B: Chemical, Elsevier, 2008, 130 (1), pp.175-180. 〈10.1016/j.snb.2007.07.123〉. 〈hal-00599950〉
  • Bruno Le Pioufle, H. Suzuki, K. Tabata, H. Noji, S. Takeuchi. Lipid Bilayer Microarray for Parallel Recording of Transmembrane Ion Currents. Analytical Chemistry, American Chemical Society, 2008, 80 (1), pp.328-332. 〈10.1021/ac7016635〉. 〈hal-00599949〉
  • E. Leclerc, B. David, L Griscom, Bruno Le Pioufle, T. Fujii, et al.. Study of Osteoblastic Cells in a Microfluidic Environment. Biomaterials, Elsevier, 2006, 27 (4), pp.558-595. 〈hal-00778216〉
  • Eric Leclerc, B. David, L. Griscom, Bruno Le Pioufle, T. Fujii, et al.. Study of osteoblastic cells in a microfluidic environment.. Biomaterials, Elsevier, 2006, 27 (4), pp.586-95. 〈10.1016/j.biomaterials.2005.06.002〉. 〈inserm-00166082〉
  • E. Leclerc, B. David, L. Griscom, Bruno Le Pioufle, T. Fujii, et al.. Study of osteoblastic cells in a microfluidic environment.. Biomaterials, Elsevier, 2006, 27, pp.586-595. 〈hal-00090179〉
  • F Morin, N. Nishimura, L Griscom, Bruno Le Pioufle, H Fujita, et al.. Constraining the connectivity of neuronal networks cultured on microelectrodes arrays with microfluidic techniques: a step towards neuron-based functional chips". Biosensors and Bioelectronics, Elsevier, 2006, 21 (7), pp.1093-1100. 〈hal-00739218〉
  • Matthieu Denoual, Y. Macé, Bruno Le Pioufle, Pascal Mognol, D. Castel, et al.. Vacuum Casting to Manufacture a Plastic Biochip for Highly Parallel Cell Transfection. Measurement Science and Technology, IOP Publishing, 2006, 17, pp.3134-3140. 〈10.1088/0957-0233/17/12/S03〉. 〈hal-00739220〉
  • E. Leclerc, B. David, L. Griscom, Bruno Le Pioufle, T. Fujii, et al.. Perfusion culture of mammalian cells in microfluidic environments for tissue engineering applications.. La Houille Blanche - Revue internationale de l'eau, EDP Sciences, 2006, 3, pp.56-59. 〈hal-00090259〉
  • E. Leclerc, B. David, L. Griscom, Bruno Le Pioufle, Y. Sakai, et al.. Perfusion culture of mammalian cells in microfluidic environments for tissue engineering applications. La Houille Blanche - Revue internationale de l'eau, EDP Sciences, 2006, pp.56-+. 〈10.1051/lhb:200603008〉. 〈hal-00795165〉
  • Matthieu Denoual, Pascal Mognol, Bruno Le Pioufle. Vacuum casting, a new answer to manufacture biomicrosystems. Journal of Engineering Manufacture, 2005, 219 (9), pp.697-502. 〈10.1243/095440505X32571〉. 〈hal-00739222〉
  • Matthieu Denoual, M. Chiral, Bruno Le Pioufle. Cell Cultures over Nanoneedle Fields. Nanobiotechnology Journal, 2005, 1 (4), pp.389-394. 〈10.1385/nbt:1:4:389〉. 〈hal-00739221〉
  • Marie Frénéa, Bruno Le Pioufle. Micromanipulation de particules polarisables par diélectrophorèse. Revue Internationale de Génie Electrique, 2004, 7 (3-4), pp.419-431. 〈10.3166/rige.7.419-431〉. 〈hal-00794466〉
  • Bruno Le Pioufle, Marie Frenea-Robin, A. Tixier. Parallel cell biochips: cell placement using dielectrophoresis or microfluidic effects. Comptes Rendus Physique, Elsevier Masson, 2004, 5 (5), pp.589-596. 〈hal-00141373〉
  • M. Frenea, S. P. Faure, Bruno Le Pioufle, P. Coquet, H. Fujita. Positioning living cells on a high-density electrode array by negative dielectrophoresis. Materials Science and Engineering: C, Elsevier, 2003, 23 (5), pp.597-603. 〈hal-00739225〉
  • Bruno Le Pioufle, M. Frénéa, A. Tixier. Biopuces pour le traitement électrique paraléllisé de cellules. Journal sur l'enseignement des sciences et technologies de l'information et des systèmes, EDP Sciences, 2003, 2 (HORS SÉRIE 2), pp.6. 〈10.1051/bib-j3ea:2003608〉. 〈hal-00739226〉
  • Laurent Griscom, Patrick Degenaar, Bruno Le Pioufle, Eiichi Tamiya, Hiroyuki Fujita. Techniques for patterning and guidance of primary culture neurons on micro-electrode arrays. Sensors and Actuators B: Chemical, Elsevier, 2002, 83 (1-3), pp.15-21. 〈hal-00778213〉
  • P. Degenaar, Bruno Le Pioufle, L. Griscom, A. Tixier, Y. Akagi, et al.. A Method for Micrometer Resolution Patterning of Primary Culture Neurons for SPM Analysis. J. Biochem, 2001, 130, pp.367-376. 〈hal-00786168〉
  • L. Griscom, P. Degenaar, Bruno Le Pioufle, E. Tamiya, H. Fujita. Cell Placement and Neural Guidance Using a Three-Dimensional Microfluidic Array. Japanese Journal of Applied Physics, Japan Society of Applied Physics, 2001, 40, pp.5485-5490. 〈hal-00778212〉
  • Patrick Surbled, Catherine Clerc, Bruno Le Pioufle, Manabu Ataka, Hiroyuki Fujita. Effect of the composition and thermal annealing on the transformation temperatures of sputtered TiNi shape memory alloy thin films. Thin Solid Films, Elsevier, 2001, 401 (1-2), pp.52-59. 〈hal-00739227〉
  • Bruno Le Pioufle, P. Surbled, H. Nagai, Y. Murakami, H. S. Chun, et al.. Living cells captured on a bio-microsystem devoted to DNA injection. Materials Science and Engineering: C, Elsevier, 2000, 12 (1-2), pp.77-81. 〈hal-00739228〉
  • P. Surbled, C. Clerc, Bruno Le Pioufle, H. Fujita. Characterization of sputtered TiNi shape memory alloy thin films. Japanese Journal of Applied Physics, Japan Society of Applied Physics, 1999, 38, pp.L1547-L1549. 〈in2p3-00003942〉
  • P. Surbled, Catherine Clerc, Bruno Le Pioufle, H. Fujita. Characterization of sputtered TiNi Shape Memory Alloy Thin Films. Japanese Journal of Applied Physics, Japan Society of Applied Physics, 1999, 38, pp.1547-1549. 〈hal-00739229〉
  • Bruno Le Pioufle, J. F. Fauvarque, P. Delalande. Comportement non linéaire des générateurs électrochimiques associés aux convertisseurs statiques. Détection de l'état de charge. European Physical Journal: Applied Physics, EDP Sciences, 1998, 2 (03), pp.257-265. 〈10.1051/epjap:1998191〉. 〈hal-00778215〉
  • D. Grenier, L. A. Dessaint, O. Akhrif, Y. Bonnassieux, Bruno Le Pioufle. Experimental nonlinear torque control of a permanent-magnet synchronous motor using saliency. IEEE Transactions on Industrial Electronics, Institute of Electrical and Electronics Engineers, 1997, 44 (5), pp.680-687. 〈10.1109/41.633471〉. 〈hal-00778211〉
  • H. Cailleux, Bruno Le Pioufle, B. Multon. COMPARISON OF CONTROL STRATEGIES TO MINIMIZE THE TORQUE RIPPLE OF A SWITCHED RELUCTANCE MACHINE. Electric Machines and Power Systems, Informa UK (Taylor & Francis), 1997, 25 (10), pp.1103-1118. 〈10.1080/07313569708955800〉. 〈hal-00778210〉
  • H. Cailleux, Bruno Le Pioufle, Bernard Multon. Modélisation et commande non linéaire en couple d'une machine à réluctance variable à double saillance. Journal de Physique III, EDP Sciences, 1996, 6 (1), pp.55-75. 〈10.1051/jp3:1996114〉. 〈jpa-00249447〉
  • J.-F. Liebaut, Bruno Le Pioufle. Onduleur MLI pour servomoteur synchrone: minimisation des pulsations de couple. RGE, 1994. 〈hal-00778217〉
  • Bruno Le Pioufle. COMPARISON OF SPEED NONLINEAR CONTROL STRATEGIES FOR THE SYNCHRONOUS SERVOMOTOR. Electric Machines and Power Systems, Informa UK (Taylor & Francis), 1993, 21 (2), pp.151-169. 〈10.1080/07313569308909644〉. 〈hal-00778214〉
  • Bruno Le Pioufle, G. Georgiou, J.-P. Louis. A robust total compensation algorithm for the torque control of a synchronous servomotor. Journal de Physique III, EDP Sciences, 1992, 2 (1), pp.129-144. 〈10.1051/jp3:1992115〉. 〈jpa-00248724〉
  • Bruno Le Pioufle, G. Georgiou. Commande non linéaire en vitesse d'un servomoteur synchrone avec calcul de trajectoire et estimation du couple résistant. Journal de Physique III, EDP Sciences, 1992, 2 (10), pp.1905-1924. 〈10.1051/jp3:1992221〉. 〈jpa-00248854〉
  • Bruno Le Pioufle, G. Georgiou, J.-P. Louis. Application des commandes non linéaires pour la régulation en vitesse ou en position de la machine synchrone autopilotée. Revue de Physique Appliquee, 1990, 25 (6), pp.517-526. 〈10.1051/rphysap:01990002506051700〉. 〈jpa-00246214〉

Communication dans un congrès80 documents

  • Tieying Xu,, Maria Lizarralde,, Wassim El Nemer, Bruno Le Pioufle, Olivier Français. Monitoring Biological Cell Flow within a Mimicking Capillary Device with Impedance Measurement. Eurosensors, Sep 2017, Paris, France. 〈10.3390/proceedings1040517〉. 〈hal-01592142〉
  • Abdelhatif El Fellahi, Thierry Bore, Lionel Rousseau, Bruno Le Pioufle, Olivier Francais. Microwave Sensor within a Microfluidic Chip for Biological Applications. Eurosensors, Sep 2017, Paris, France. MDPI, 1 (4), pp.523, 2017, Proceedings of Eurosensors Conference. 〈10.3390/proceedings1040523〉. 〈hal-01592115〉
  • Bruno Le Pioufle, Olivier Français. Mechanical and Electrical sensing of single biological cell properties within microfluidic devices. NEMS (Nano Engineering Molecular Systems), Apr 2017, Los-Angeles, United States. 〈hal-01515021〉
  • Clyde Midelet, J.-Y. Lin, Sung Tsang, Chen-Li Sun, Johanna Midelet, et al.. Effects of biomolecules on the electrokinetics of colloidal nanoparticles in liquid suspension. Marek Osiński, Wolfgang J. Parak, Xing-Jie Liang. SPIE BIOS, Jan 2017, San Francisco, CA, United States. Proc. SPIE, 10078, pp.100780T, 2017, Colloidal Nanoparticles for Biomedical Applications XII. 〈10.1117/12.2252470〉. 〈hal-01501667〉
  • Jean Roman, Olivier Francais, Nathalie Jarroux, Gilles Patriache, Juan Pelta,, et al.. Nanoparticle electrical analysis and detection with a solid-state nanopore in a microfluidic device. Eurosensors 2016, Sep 2016, Budapest, Hungary. 〈hal-01390031〉
  • Olivier Français, Bruno Le Pioufle. Microfluidic devices for the cell sensing and treatment. GLEAM'15, Jun 2015, Cachan, France. 〈hal-01222696〉
  • Claudia Irene Trainito, Emilie Bayart, Emilie Bisceglia, Frédéric Subra, Olivier Français, et al.. Electrorotation as a Versatile Tool to Estimate Dielectric Properties of Multi-scale Biological Samples: from Single Cell to Spheroid Analysis. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies, Sep 2015, Portoroz, Slovenia. Springer Singapore, 53, pp.75-78, 2015, IFMBE Proceedings. 〈http://link.springer.com/chapter/10.1007/978-981-287-817-5_17#page-1〉. 〈hal-01222447〉
  • Pierre Bodénès, Filipa Lopes, Dominique Pareau, Olivier Français, Bruno Le Pioufle. A Microfluidic Device for the Real-Time Characterization of Lipid Producing Algae Cell Population Submitted to a Pulsed Electric Field. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies, Sep 2015, Portorož, Slovenia. Springer Singapore, 53, pp.409-413, IFMBE procedings. 〈http://link.springer.com/chapter/10.1007/978-981-287-817-5_89〉. 〈10.1007/978-981-287-817-5_89〉. 〈hal-01222446〉
  • Lionel Rousseau, Gaelle Lissorgues, Fikar Pavel, Olivier Francais, Bruno Le Pioufle, et al.. SU-8 microchannels for live cell dielectrophoresis improvements. DTIP 2015, Apr 2015, Montpellier, France. 〈hal-01159000〉
  • Franz Bozsak, Bruno Carreel, Pierluca Massina, Myline Cottance, Olivier Francais, et al.. First in vivo results of an innovative sensor to detect stent-related complications. ICI 2015, Dec 2015, Tel-Aviv, Israel. 2015. 〈hal-01275165〉
  • Pierre Bodénès, Sakina Bensalem, Filipa Lopes, Dominique Pareau, Olivier Francais, et al.. In situ and real time electroporation of a lipid producing microalgae in a microfluidic device. Algae Europe 2015, Dec 2015, Lisbonne, Portugal. 〈hal-01275167〉
  • Agnes Tixier, E. Lebrasseur, T. Takahashi, Olivier Francais, Bruno Le Pioufle, et al.. Compressively-Stressed Test Structures for Opaque Micro-Structures Releasing Visualization. International Conference on Microelectronic Test Structures, Mar 2014, Udine, Italy. pp.170-173, 2014. 〈hal-00987105〉
  • Agnes Tixier, Takuya Takahashi, Hiroyuki Fujita, H. Toshiyoshi, Isao Mori, et al.. Detection of Micro-Beads by Impedance Cytometry - Towards a Wholly Integrated Electronic Device for Biological Cells Applications. Symposium on Design, Test, Integration ans Packaging of MEMS/MOEMS, Apr 2014, Cannes, France. pp.1-6, 2014. 〈hal-00987015〉
  • Wassim El Nemer, Papa Alioune Ndour, Sophie D. Lefevre, Harvey Tawfik, Julien Picot, et al.. A microfluidic device to study red blood cell deformability. 4th European Conference on Microfluidics, Dec 2014, Limerick, Ireland. 〈hal-01113061〉
  • Claudia Trainito, Olivier Francais, Bruno Le Pioufle. Monitoring in real time the dielectric properties of a single cell combining dielectrophoresis and electrorotation experiments in a microfluidic device : towards electropermeabilization analysis. Dielectrophoresis 2014, Jul 2014, United Kingdom. 2014. 〈hal-01026163〉
  • Claire Dalmay, Emilie Bisceglia, Feriel Hamdi, Olivier Francais, Bruno Le Pioufle. Micro-nanotechnologies pour l’analyse et le traitement parallèle de cellules ou de protéines sur puce. Symposium de Génie Electrique, Jul 2014, Cachan, France. 2014. 〈hal-01076067〉
  • Olivier Français, Bruno Le Pioufle, Claudia Trainito. Etude et mise en oeuvre d’un microsystème fluidique pour la caractérisation diélectrique de cellules biologiques par électrorotation. Symposium de Génie Electrique, Jul 2014, Cachan, France. 〈hal-01076066〉
  • Olivier Francais, Bruno Le Pioufle. Apport des microtechnologies pour l’étude des propriétés diélectriques cellulaires : application au tri et à la capture de cellules biologiques en environnement microfluidique. Congrès Annuels conjoints de la Société Français d'Immunologie (SFI) et de l'Association Française de Cytométrie (AFC), Nov 2014, Lille, France. 〈hal-01085273〉
  • Rémi Sieskind, Claudia Trainito, Olivier Francais, Bruno Le Pioufle. Microsystème dédié à l’étude de la polarisation diélectrique de micro-particules dans le cadre de formation master recherche : application au micro-positionnement 3D de cellules par force de diélectrophorèse. CETSIS 2014 : Enseignement des Technologies et de Sciences de l'Information et des Systèmes, Oct 2014, Besançon, France. 〈hal-01085282〉
  • Claudia Trainito, Olivier Francais, Bruno Le Pioufle. A microfluidic device to determine dielectric properties of a single cell : a combined dielectrophoresis and electrorotation technique. 4th European Conference on Microfluidics, Dec 2014, Limerick, Ireland. 〈hal-01096921〉
  • Bertrand David, Eric Perrin, Lazaar Mélanie, Chabanon Morgan, Bruno Le Pioufle, et al.. Reproducing topography and roughness of osteoconductive materials in a microfluidic device for bone tissue engineering. 4th European Conference on Microfluidics, Dec 2014, Limerick, Ireland. 〈hal-01096932〉
  • Feriel Hamdi, Wei Wang, Frédéric Subra, Elisabeth Dufour-Gergam, Olivier Français, et al.. High Density Cell Electrofusion on Chip Using an Array of Non-Connected Metallic Pads. Biodevices, 2013, Barcelone, Spain, Spain. pp.68-72, 2013. 〈hal-00790205〉
  • Agnes Tixier, Isao Mori, T. Takahashi, Olivier Francais, Bruno Le Pioufle, et al.. Micro-fluidic channel integration on thick-SOI LSI device for cells analyses. Sensor Symposium 2013, Nov 2013, Sendaï, Japan. pp.320-325. 〈hal-00987092〉
  • Olivier Francais, Bruno Le Pioufle. La mesure de la bio-impédance d'un végétal comme moyen d'introduire le domaine de la biologie dans une formation E.E.A.. Colloque sur l'Enseignement des Technologies et des Sciences de l'Information et des Systèmes (CETSIS), Mar 2013, Caen, France. pp.199 - 203, 2013. 〈hal-00804673〉
  • Bruno Le Pioufle, Claire Dalmay, Olivier Francais. Design and use of microfluidic devices for the real time monitoring of micro/nanopulses effect on cells. Bioelectrochemistry 2013, Mar 2013, Bochum, Germany. pp.80, 2013. 〈hal-00805500〉
  • Martinus H. V. Werts, Matthieu Loumaigne, Julien R. G. Navarro, A. Débarre, Laurent Griscom, et al.. Quenched fluorescence and enhanced resonant light scattering in molecular plasmonic assemblies. Molecular Plasmonics 2013, May 2013, Jena, Germany. 〈hal-00877127〉
  • Martinus H. V. Werts, Matthieu Loumaigne, Julien R. G. Navarro, Anne Débarre, Laurent Griscom, et al.. Quenched fluorescence and enhanced resonant light scattering in molecular plasmonic assemblies. International Conference on Photochemistry 2013, Jul 2013, Belgium. 〈hal-00877131〉
  • Martinus H. V. Werts, Vincent Raimbault, Matthieu Loumaigne, Laurent Griscom, Olivier Francais, et al.. Optical microscopy and spectroscopy of analyte-sensitive functionalized gold nanoparticles in microfluidic systems. Proc. SPIE 2013, 8595, 85950W, Feb 2013, United States. 8595, pp.85950W, 2013, 〈10.1117/12.2001608〉. 〈hal-00809421v2〉
  • Emilie Bisceglia, Myriam Cubizolles, Frédéric Mallard, Olivier Français, Bruno Le Pioufle. Dielectrophoretic separation of blood pathogens ou Bacterial extraction from biological samples using DEP forces. lab on a chip world congress, Sep 2012, San Diego, United States. pp.1, 2012. 〈hal-00786577〉
  • Martinus H. V. Werts, Julien R. G. Navarro, Laurent Griscom, Olivier Francais, Bruno Le Pioufle, et al.. From fluorescence quenching by metal nanoparticles to enhanced spectroscopic detection in microfluidic volumes. 1st International Conference on Enhanced Spectroscopy, Oct 2012, Porquerolles, France. 〈hal-00753361〉
  • M. Chabanon, H. Duval, O. Francais, Bruno Le Pioufle, E. Vennat, et al.. In vitro and in silico study of cell growth in porous scaffold under dynamic flow. 3rd TERMIS World Congress 2012 - Vienna, Austria, Sep 2012, Vienna, Austria. WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA, 6 (Suppl. 1), pp.340-341, 2012. 〈hal-00741428〉
  • Marie-Amélie De Ménorval, Franck André, Aude Silve, Claire Dalmay, O. Francais, et al.. Development and uses of a microfluidic chamber to apply microsecond and nanosecond electric pulses and to visualize biological effects induced. Pôle Lorrain de l'Ingéniérie du Cartilage, Sep 2011, Nancy, France. pp.1, 2011. 〈hal-00933141〉
  • Marie-Amélie De Ménorval, Franck André, Aude Silve, Claire Dalmay, Olivier Français, et al.. Development and uses of a microfluidic chamber to apply microsecond or nanosecond electric pulses and to visualize biological effects induced. Electroporation based Technologies and Treatments, Nov 2011, Ljubljana, Slovenia. pp.1, 2011. 〈hal-00933139〉
  • Marie-Amélie De Ménorval, Franck Andre, Aude Silve, Claire Dalmay, Olivier Français, et al.. Dispositifs microfluidiques pour l'exposition et l'observation de cellules biologiques exposées à des impulsions électriques nanosecondes. Journées Nationales des Nanotechnologies et des Nanosciences, J3N, Nov 2011, Strasbourg, France. pp.1, 2011. 〈hal-00933138〉
  • Feriel Hamdi, Olivier Francais, Julien Villemejane, M Woytasik, Claire Dalmay, et al.. A Microfluidic Biochip Dedicated to Highly Parallelized Electrofusion. MMB, 2011, Lucerne, Switzerland. 2011. 〈hal-00739182〉
  • Fériel Hamdi, Olivier Francais, Marion Woytasik, Elisabeth Dufour-Gergam, Bruno Le Pioufle. Self control of cell electroporation by dielectrophoresis transition. Nanobiotech, 2011, Montreux, Switzerland. 2011. 〈hal-00739183〉
  • S Michelet, J-P Barbot, O Francais, P.Y. Joubert, P Larzabal, et al.. Towards a Statistical Description of Experimental Data for Detection - Estimation problems in DNA translocations trhough nanopores. BIOSIGNALS, 2011, Roma, Italy. 2011. 〈hal-00739181〉
  • Claire Dalmay, Olivier Francais, Frédéric Subra, Bruno Le Pioufle. Insulated gold micro singularities for high density cell trapping based on dielectrophoresis. Nanobiotech, 2011, Montreux, Switzerland. 2011. 〈hal-00739186〉
  • M.A. De Menorval, F Andre, Aude Silve, Claire Dalmay, O Francais, et al.. Development and uses of a microfluidic chamber to apply microsecond or nanosecond electric pulses and to visualize biological effects induced.. PLIC - Electroporation based Technologies and Treatments, 2011, Lubjana, Slovenia. 2011. 〈hal-00739184〉
  • Claire Dalmay, Yung-Yung Lai, Laurent Griscom, Olivier Français, Bruno Le Pioufle, et al.. Positioning and orientation of adherent cells in a microfluidic chip using the micro patterning of a parylen-C-film. biodevices, 2011, Rome, Italy. 2011. 〈hal-00739185〉
  • Claire Dalmay, Olivier Francais, Bruno Le Pioufle. Array of Metallic Singularities for the high density cell Placement on a Microfluidic Chip. MMB, 2011, Lucerne, Switzerland. 2011. 〈hal-00739187〉
  • N Pham Van, J Villemejane, F Hamdi, G Mottet, Claire Dalmay, et al.. A High Density Microfluidic Device for Cell Pairing and Electrofusion. Eurosensors, 2010, Linz, Austria. 2010. 〈hal-00739189〉
  • Claire Dalmay, J Villemejane, O Français, M Woytasik, E Dufour-Gergam, et al.. Thick Electrode Biochip for in Vitro Studies of the Nanoporation of Living Cells. Microfluidics, 2010, Toulouse, France. 2010. 〈hal-00739191〉
  • Toshihisa Osaki, Jean-Pierre Barbot, Ryuji Kawano, Hirotaka Sasaki, Olivier Français, et al.. A Rupture Detection Algorithm for the DNA Translocation Detection Though Biological Nanopore. Eurosensors, 2010, Linz, Austria. 2010. 〈hal-00739190〉
  • J Villemejane, G Mottet, O Français, Jp Lefèvre, M Woytasik, et al.. Nanomanipulation of living cells on a chip using electric field: general concepts and microdevices. DELTA, 2010, Ho-shi-minh, Vietnam. 2010. 〈hal-00739188〉
  • O Français, G Mottet, A Ayina, Bruno Le Pioufle. 3D field focusing and defocusing geometries for cell trapping on a chip. DTIP, 2009, Roma, Italy. 2009. 〈hal-00739193〉
  • J Villemejane, V. Joubert, O Français, Bruno Le Pioufle, Lm Mir. Nanosecond electric pulses-induced enhancement of reporter gene expression after electrotransfer in skeletal muscle in vivo. Int. Bioelectric Symposium, 2009, France. 2009. 〈hal-00739192〉
  • O Français, B Mahe, A Dimitracopoulos, Mc Julien, Jp Lefevre, et al.. Digital concentration gradient generators on a microfluidic chip. DTIP, 2009, Rome, Italy. 2009. 〈hal-00739194〉
  • O Francais, A Dimitracopoulos, Jp Lefevre, F Subra, Jf Mouscadet, et al.. Fast concentration gradient generator on a cell biochip for toxicology applications. MicroTAS, 2009, France. 2009. 〈hal-00739195〉
  • O. Francais, A Ayina, J Villemejane, L Rousseau, L Mir, et al.. High Density Cell Microarray Based on Dielectrophoresis Traps Induced by a Matrix of Singularities. MicroTAS, 2009, North Korea. 2009. 〈hal-00739196〉
  • B. Hajj, S. De Reguardati, L. Hugonin, T. Osaki, H. Suzuki, et al.. Electro-Optical Imaging Microscopy of Dye Doped Lipid Bilayer. 2009 Conference on Lasers and Electro-Optics and Quantum Electronics and Laser Science Conference, 2009, Germany. pp.3-4, 2009. 〈hal-00795108〉
  • J Villemejane, G Mottet, O Français, J.P. Lefevre, L.M. Mir, et al.. Insulated Liquid Electrodes in a microfluidic chip for the electroporation of living cells. European Conference on Microfluidics, 2008, Bologna, Italy. 2008. 〈hal-00739197〉
  • T Osaki, H Suzuki, Bruno Le Pioufle, S Takeuchi. Multichannel Nanopore for High Throughput Single Molecule Detection. MicroTAS, 2008, North Korea. 2008. 〈hal-00739198〉
  • G Mottet, J Villemejane, J.P. Lefevre, Bruno Le Pioufle. A complete process to make a lab on a chip with complex fluidics and thick electrodes for biological applications. European Conference on Microfluidics, 2008, Bologne, Italy. 2008. 〈hal-00739199〉
  • Marie Frenea-Robin, Julie Laforet, G. Robin, Bruno Le Pioufle, Laurent Nicolas. Selecting an electrode structure for cell sorting by differential dielectric affinity. 29TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY, Aug 2007, Lyon, France. pp.6311-6314, 2007, IEEE Engineering in Medicine and Biology Society Conference Proceedings. 〈10.1109/IEMBS.2007.4353798〉. 〈hal-00412972〉
  • Y. H. Cho, S. W. Lee, Bruno Le Pioufle, N. Takama, S. Takeuchi, et al.. High-throughput single-cell electroporation microchip with three dimensional si microelectrodes for gene transfection. Transducers '07 & Eurosensors Xxi, Digest of Technical Papers, Vols 1 and 2, 2007, France. pp.U474-U475, 2007. 〈hal-00795167〉
  • Matthieu Denoual, Magali Chiral, Olivier De Sagazan, Bruno Le Pioufle. Nanoneedle fields for biological interaction with cells. Proc. of Micro Total Analysis System (uTAS), Japan, 2006, 2006, Japan. pp 1-9, 2006. 〈hal-00986481〉
  • Magali Chiral, Matthieu Denoual, Antonin Hoël, Jimmy Aguinet, Bruno Le Pioufle. Electrotransfection results on Jurkat cells on a microdevice. Angioskin, Electroporation based Technologies and Treatments workshop, Slovenia, 2005, 2005, Slovenia. 〈hal-00986477〉
  • Matthieu Denoual, Magali Chiral, Olivier De Sagazan, Bruno Le Pioufle. Cell Cultures over Nanoneedle Fields Euronanoforum. Euronanoforum, Nanotechnology and the Health of EU Citizen, 2005, United Kingdom. 〈hal-00986470〉
  • Olivier De Sagazan, Matthieu Denoual, P. Guil, D. Gaudin, Bruno Le Pioufle, et al.. Horizontal buried channels in monocrystalline silicon. Conf. Proc. of DTIP of MOEMS & MEMS 2005, May 2005, Montreux, Switzerland. pp.351-354, 2005. 〈hal-00775898〉
  • E. Leclerc, L. Griscom, Bruno Le Pioufle, C. Guguen-Guillouzo, A. Corlu, et al.. Investigation of Human Hepatoma cell line microfluidic devices.. 3rd annual International IEEE EMBS special topic conference on microtechnologies in medicine and biology., 2005, Oahu, Hawaii, United States. 2005. 〈hal-00019381〉
  • L. Griscom, G. Legeay, Bruno Le Pioufle. Surface engineering of microfluidic systems for cellular biochips. 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology, 2005, China. pp.194-197, 2005, 〈10.1109/mmb.2005.1548424〉. 〈hal-00795194〉
  • L. Griscom, Y. Chateau, J. P. Pennec, L. Misery, Bruno Le Pioufle. Co-culture of cells in PDMS microsystem for sensitized artificial skin. 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology, 2005, China. pp.184-187, 2005, 〈10.1109/mmb.2005.1548421〉. 〈hal-00795208〉
  • L. Griscom, Y. Chateau, J. P. Pennec, L. Misery, Bruno Le Pioufle. Microsystems for dermatological research. Journal of Investigative Dermatology, 2005, Australia. 125, pp.A20-A20, 2005. 〈hal-00795213〉
  • Matthieu Denoual, S. Prioux, Y. Mace, Pascal Mognol, Bruno Le Pioufle. Vacuum casting of low-cost polymer microstructures for bio-chemical microsystems applications. Micro Total Analysis Systems 2004, Vol 2, 2005, Netherlands. pp.357-359, 2005, Royal Society of Chemistry Special Publications. 〈hal-00795218〉
  • E. Leclerc, B. David, R. Warocquier-Clerout, L. Griscom, Bruno Le Pioufle, et al.. Behaviour of osteoblast-like cells in cellular microdevices. Micro Total Analysis Systems 2004, Vol 1, 2005, Netherlands. pp.396-398, 2005, Royal Society of Chemistry Special Publications. 〈hal-00795188〉
  • Matthieu Denoual, Pascal Mognol, Bruno Le Pioufle. Exploring the Limits of Vacuum Casting Technique for Micron and Submicron Features. Proc. of Solid Freeform Symposium, 2004, United States. pp 1-9, 2004. 〈hal-00986465〉
  • Olivier De Sagazan, Matthieu Denoual, P. Guil, D. Gaudin, Bruno Le Pioufle, et al.. Horizontal buried channels in monocrystalline silicon. Proceeding of the MEMS 2004 conference, Jan 2004, Maastricht, Germany. pp.661-664, 2004. 〈hal-00847215〉
  • Matthieu Denoual, Olivier De Sagazan, Patrice Guil, Bruno Le Pioufle. Driving Liquids with Thermal Actuators. Proc. of Actuator, 9th conference, Bremen, 2004, Germany. pp 1-9, 2004. 〈hal-00986466〉
  • Marie Frenea-Robin, M. Chiral, Bruno Le Pioufle, Nathalie Melaine, C. Pineau, et al.. On-chip spermatozoa trapping by dielectrophoresis. 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Sep 2004, Malmo, Sweden. 1 (296), pp.111-113, 2005, ROYAL SOCIETY OF CHEMISTRY SPECIAL PUBLICATIONS. 〈hal-00412976〉
  • A. Tixier-Mita, J. Jun, M. Chiral, S. Ostrovidov, Marie Frenea-Robin, et al.. A silicon micro-system for parallel gene transfection into arrayed cells. 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Sep 2004, Malmo, Sweden. 2 (297), pp.180-182, 2005, ROYAL SOCIETY OF CHEMISTRY SPECIAL PUBLICATIONS. 〈hal-00412979〉
  • Fabrice Morin, Matthieu Denoual, Laurent Griscom, Bruno Le Pioufle, Hiroyuki Fujita, et al.. Controlling Cell Development by Microfluidic Techniques : a Step towards Whole-Cell Biosensors with Defined Biological Features. 6th Micro Total Analysis System (uTAS), 2002, Japan. pp.10.1007, 2002. 〈hal-00986459〉
  • Laurent Griscom, Patrick Degenaar, Matthieu Denoual, Fabrice Morin, Bruno Le Pioufle, et al.. Culturing of Neurons in Microfluidic Arrays. 2nd IEEE-EMBS Special Topic Conference on Microtechnologies in Medecine&Biology, 2002, United States. pp 160 - 163, 2002, 〈10.1109/MMB.2002.1002305〉. 〈hal-00986450〉
  • M. Frenea, Hervé Lhermite, Bruno Le Pioufle, H. Fujita. Design of biochip microelectrode arrays for cell arrangement. 2nd Annual International Ieee-Embs Special Topic Conference on Microtechnologies in Medicine & Biology, Proceedings, 2002, United States. pp.140-143, 2002, 〈10.1109/mmb.2002.1002281〉. 〈hal-00795221〉
  • Gonzalo Cabodevila, Marie Frénéa, Agnes Tixier, Katel Cozic, Bruno Le Pioufle, et al.. Arrayed Micro Needles and Electrodes for Aligned Cell Capture and Gene Insertion. Chemicro, Mar 2002, Kyoto, Japan. 2002. 〈hal-00107155〉
  • Gonzalo Cabodevila, Hiroyuki Fujita, Bruno Le Pioufle. Arrayed Micro Needles for Mechanical Gene Insertion. Proceedings of SPIE -- Volume 4937 Biomedical Applications of Micro- and Nanoengineering,, Nov 2002, Melbourne, Australia. pp.90-97, 2002, 〈10.1117/12.473962〉. 〈hal-00107288〉
  • G. Cabodevila, Bruno Le Pioufle, H. Fujita. Arrayed micro needles for mechanical gene insertion. Biomedical Applications of Micro- and Nanoengineering, 2002, Australia. 4937, pp.90-97, 2002, Proceedings of the Society of Photo-Optical Instrumentation Engineers (Spie). 〈hal-00795248〉
  • L. Griscom, P. Degenaar, Bruno Le Pioufle, E. Tamiya, H. Fujita. Soft lithographic techniques for guidance of hippocampal neurons on micro-electrode arrays. Transducers '01: Eurosensors Xv, Digest of Technical Papers, Vols 1 and 2, 2001, Germany. pp.338-341, 2001. 〈hal-00795227〉
  • A. Tixier, L. Griscom, K. Cozic, H. Nagai, Bruno Le Pioufle, et al.. Catching and attaching cells using an array of microholes. 1st Annual International Ieee-Embs Special Topic Conference on Microtechnologies in Medicine & Biology, Proceedings, 2000, France. pp.36-40, 2000, 〈10.1109/mmb.2000.893736〉. 〈hal-00795228〉
  • Sandrine Hallaert, Emmanuel Sarraute, Bruno Le Pioufle. Numerical and Analytical Modeling of the Piezoelectric Transformer and Experimental Verification. Nanotech, 1999, France. pp.198-201, 1999. 〈hal-00794999〉