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Equipe SeeDev, UMR 1318 Institut Jean Pierre Bourgin, INRA, centre de Versailles

From my past and present research achievements, I have acquired a good expertise in plant molecular genetics. My technical skills are based on the tools of molecular biology, cytology and bioinformatics and serve to answer biological questions involving seed development. My main topic is the transcriptional network controlling the switch between embryogenesis and accumulation of reserves. I am focusing on LEC2, a master regulator of seed maturation belonging to the B3 family. My work aims to determine (i) how this factor binds, or interact, with its DNA targets, in a possible complex with ABI3 and FUS3, (ii) how is this protein complex is regulated, and (iii) how LEC2 is transcriptionnaly and post-transcriptionally tuned. We aim at modelling the regulatory network involved in Arabidopsis and  investigate how it is conserved in cereals.

Scientific manager of the cytology and imaging platform of the Plant Observatory, I ensure the proper scientific and human functioning of a strategic tool for my institute (1200M2, 5 Full Time Equivalents, 100 regular users, 5 confocal micoscopes, laser microdissector, twenty large optic pieces, and various cytology-related materials
Operating correspondent for the institute, I manage all aspects of the unit's 9 buildings (8000M2),  requests for equipment, works, modifications, in relations with the research center for all these aspects. I am also managing the unit’s workshop (3 FTEs).



Journal articles43 documents

  • Pierre Jacob, Gwilherm Brisou, Marion Dalmais, Johanne Thévenin, Froukje van der Wal, et al.. The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis. Genes, MDPI, 2021, 12 (5), pp.1-18. ⟨10.3390/genes12050746⟩. ⟨hal-03335244⟩
  • Thomas James Roscoe, Virginie Vaissayre, Gaël Paszkiewicz, Fernando Clavijo, Zsolt Kelemen, et al.. Regulation of FUSCA3 Expression During Seed Development in Arabidopsis. Plant and Cell Physiology, Oxford University Press (OUP), 2019, 60 (2), pp.476-487. ⟨10.1093/pcp/pcy224⟩. ⟨hal-02386031⟩
  • Charlotte Miller, Rachel Wells, Neil Mckenzie, Martin Trick, Joshua Ball, et al.. Variation in expression of the HECT E3 ligase UPL3 modulates LEC2 levels, seed size, and crop yields in brassica napus. The Plant cell, American Society of Plant Biologists (ASPB), 2019, 31 (10), pp.2370-2385. ⟨10.1105/tpc.18.00577⟩. ⟨hal-02622699⟩
  • L. Lepiniec, M. Devic, T. Roscoe, D. Bouyer, D.-X Zhou, et al.. Molecular and epigenetic regulations and functions of the LAFL transcriptional regulators that control seed development. Sexual Plant Reproduction, Springer Verlag, 2018, 31 (3), pp.291-307. ⟨10.1007/s00497-018-0337-2⟩. ⟨hal-01969458⟩
  • Kaori Sakai, Ludivine Taconnat, Néro Borrega, Jennifer Yansouni, Véronique Brunaud, et al.. Combining laser-assisted microdissection (LAM) and RNA-seq allows to perform a comprehensive transcriptomic analysis of epidermal cells of Arabidopsis embryo. Plant Methods, BioMed Central, 2018, 14, pp.10. ⟨10.1186/s13007-018-0275-x⟩. ⟨hal-02625394⟩
  • Céline Boulard, Johanne Thévenin, O. Tranquet, V. Laporte, L. Lepiniec, et al.. LEC1 (NF-YB9) directly interacts with LEC2 to control gene expression in seed. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms , Elsevier, 2018, 1861 (5), pp.443-450. ⟨10.1016/j.bbagrm.2018.03.005⟩. ⟨hal-01746034⟩
  • Céline Boulard, Abdelhak Fatihi, Loic Lepiniec, Bertrand Dubreucq. Regulation and evolution of the interaction of the seed B3 transcription factors with NF-Y subunits. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms , Elsevier, 2017, 1860 (10), pp.1069-1078. ⟨10.1016/j.bbagrm.2017.08.008⟩. ⟨hal-02627256⟩
  • Mark Tepfer, Bertrand Dubreucq, Jean-Denis Faure. La caméline, une plante modèle pour la recherche translationnelle. Biofutur, Elsevier - Cachan : Lavoisier, 2016, pp.38-41. ⟨hal-01529235⟩
  • Sebastien Baud, Zsolt Kelemen, Johanne Thévenin, Céline Boulard, Sandrine Blanchet, et al.. Deciphering the molecular mechanisms underpinning the transcriptional control of gene expression by L-AFL proteins in Arabidopsis seed.. Plant Physiology, American Society of Plant Biologists, 2016, 171 (171), pp.1099-1112. ⟨10.1104/pp.16.00034⟩. ⟨hal-01406124⟩
  • Sébastien Baud, Abdelhak Fatihi, Celine Boulard, Daniel Bouyer, Sebastien Baud, et al.. Deciphering and modifying LAFL transcriptional regulatory network in seed for improving yield and quality of storage compounds. Plant Science, Elsevier, 2016, 250, pp.198-204. ⟨10.1016/j.plantsci.2016.06.013⟩. ⟨hal-02356216⟩
  • Johanne Thevenin, Wenjia Xu, Louise Vaisman, Loic Lepiniec, Bertrand Dubreucq, et al.. The Physcomitrella patens System for Transient Gene Expression Assays.. Methods in Molecular Biology, Humana Press/Springer Imprint, 2016, 1482, pp.151-61. ⟨10.1007/978-1-4939-6396-6_10⟩. ⟨hal-01389059⟩
  • Celine Boulard, Michel Bardet, Thierry Chardot, Bertrand Dubreucq, Marina Gromova, et al.. The structural organization of seed oil bodies could explain the contrasted oil extractability observed in two rapeseed genotypes.. Planta, Springer Verlag, 2015, 242 (1), pp.53-68. ⟨10.1007/s00425-015-2286-4⟩. ⟨hal-01204182⟩
  • Zsolt Kelemen, Alvaro Sebastian, Wenjia Xu, Damaris Grain, Fabien Salsac, et al.. Analysis of the DNA-Binding Activities of the Arabidopsis R2R3-MYB Transcription Factor Family by One-Hybrid Experiments in Yeast. PLoS ONE, Public Library of Science, 2015, 10 (10), pp.e0141044. ⟨10.1371/journal.pone.0141044⟩. ⟨hal-01837546⟩
  • Martine Miquel, Ghassen Trigui, Alain Trubuil, Bertrand Dubreucq. Imaging and quantitative analysis of size and distribution of sperical bodies, [i]e.g[/i]. embryonic oil bodies. Bio-protocol , Bio-protocol LCC, 2015, 5 (1), pp.1-6. ⟨hal-01204094⟩
  • Aurélie Grimault, Ghislaine Gendrot, Sophy Chamot, Thomas Widiez, Hervé Rabillé, et al.. ZmZHOUPI, an endosperm-specific basic helix-loop-helix transcription factor involved in maize seed development. Plant Journal, Wiley, 2015, 84 (3), pp.574-586. ⟨10.1111/tpj.13024⟩. ⟨hal-01536562⟩
  • Aurélie Grimault, Ghislaine Gendrot, Sandrine Chaignon, Françoise Gilard, Guillaume Tcherkez, et al.. Role of B3 domain transcription factors of the AFL family in maize kernel filling. Plant Science, Elsevier, 2015, 236, pp.116-125. ⟨10.1016/j.plantsci.2015.03.021⟩. ⟨hal-01204180⟩
  • Gregoire Denay, Audrey Creff, Steven Moussu, Pauline Wagnon, Johanne Thevenin, et al.. Endosperm breakdown in Arabidopsis requires heterodimers of the basic helix-loop-helix proteins ZHOUPI and INDUCER OF CBP EXPRESSION 1. Development (Cambridge, England), Company of Biologists, 2014, 141 (6), pp.1222-U85. ⟨10.1242/dev.103531⟩. ⟨hal-01204028⟩
  • Laure Aymé, Sebastien Baud, Bertrand Dubreucq, Florent Joffre, Thierry Chardot. Function and localization of the Arabidopsis thaliana diacylglycerol acyltransferase DGAT2 expressed in yeast. PLoS ONE, Public Library of Science, 2014, 9 (3), 9 p. ⟨10.1371/journal.pone.0092237⟩. ⟨hal-01204016⟩
  • Martine Miquel, Ghassen Trigui, Sabine d'Andrea, Zsolt Kelemen, Sebastien Baud, et al.. Specialization of oleosins in OB dynamics during seed development in Arabidopsis thaliana seeds.. Plant Physiology, American Society of Plant Biologists, 2014, 164 (4), pp.1866-1878. ⟨10.1104/pp.113.233262⟩. ⟨hal-01019614⟩
  • Guillaume Barthole, Alexandra To, Chloé Marchive, Veronique Brunaud, Ludivine Soubigou-Taconnat, et al.. MYB118 Represses Endosperm Maturation in Seeds of Arabidopsis. The Plant cell, American Society of Plant Biologists (ASPB), 2014, 26 (9), pp.3519 - 3537. ⟨10.1105/tpc.114.130021⟩. ⟨hal-01204153⟩
  • Francisco Cubillos, Jennifer Yansouni, Ahmid Khalili, Sandrine Balzergue, Samira Elftieh, et al.. Expression variation in connected recombinant populations of Arabidopsis thaliana highlights distinct transcriptome architectures. BMC Genomics, BioMed Central, 2012, 13, pp.1-12. ⟨10.1186/1471-2164-13-117⟩. ⟨hal-01189696⟩
  • Johanne Thevenin, Céline Dubos, W. Xu, Jose-Sabrina Le Gourrierec, Zoltan Kelemen, et al.. A new system for fast and quantitative analysis of heterologous gene expression in plants. New Phytologist, Wiley, 2012, 193 (2), pp.504 - 512. ⟨10.1111/j.1469-8137.2011.03936.x⟩. ⟨hal-01004263⟩
  • Fabienne Guillon, Colette Larre, Fabrice Petitpas, Adeline Berger, Jihad Moussawi, et al.. A comprehensive overview of grain development in Brachypodium distachyon variety Bd21. Journal of Experimental Botany, Oxford University Press (OUP), 2012, 63 (2), pp.739-755. ⟨10.1093/jxb/err298⟩. ⟨hal-01190419⟩
  • Ghassen Trigui, Béatrice Laroche, Martine Miquel, Bertrand Dubreucq, Alain Trubuil. The dynamics of oil bodies in Arabidopsis thaliana seeds : a mathematical model of biogenesis and coalescence. International Journal of Electrical, Computer, Energetic, Electronic and Communication Engineering, World Academy of Science Engineering and Technology, 2012, 64, pp.763-768. ⟨hal-02643123⟩
  • Nathalie Berger, Bertrand Dubreucq. Evolution goes GAGA: GAGA binding proteins across kingdoms. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms , Elsevier, 2012, 1819 (8), pp.863 - 868. ⟨10.1016/j.bbagrm.2012.02.022⟩. ⟨hal-01004142⟩
  • Nathalie Berger, Bertrand Dubreucq, François Roudier, Christian Dubos, Loic Lepiniec. Transcriptional regulation of Arabidopsis LEAFY COTYLEDON2 involves RLE,a cis-element that regulates Trimethylation of Histone H3 at Lysine-27. The Plant cell, American Society of Plant Biologists (ASPB), 2011, 23 (11), pp.4065-4078. ⟨10.1105/tpc.111.087866⟩. ⟨hal-01000017⟩
  • Erwana Harscoet, Bertrand Dubreucq, Jean-Christophe Palauqui, Loic Lepiniec. NOF1 encodes an arabidopsis protein involved in the control of rRNA expression. PLoS ONE, Public Library of Science, 2010, 5 (9), pp.e12829. ⟨10.1371/journal.pone.0012829⟩. ⟨hal-01203890⟩
  • Helen North, Sebastien Baud, Isabelle Debeaujon, Christian Dubos, Bertrand Dubreucq, et al.. Arabidopsis seed secrets unravelled after a decade of genetic and omics-driven research. Plant Journal, Wiley, 2010, 61 (6), pp.971-981. ⟨10.1111/j.1365-313X.2009.04095.x⟩. ⟨hal-01203892⟩
  • Monica Santos-Mendoza, Bertrand Dubreucq, Sebastien Baud, François Parcy, Michel Caboche, et al.. Deciphering gene regulatory networks that control seed development and maturation in Arabidopsis.. Plant Journal, Wiley, 2008, 54 (4), pp.608-20. ⟨10.1111/j.1365-313X.2008.03461.x⟩. ⟨hal-00297574⟩
  • Elisabeth Truernit, Hélène Bauby, Bertrand Dubreucq, Olivier Grandjean, John Runions, et al.. High-resolution whole-mount imaging of three-dimensional tissue organization and gene expression enables the study of Phloem development and structure in Arabidopsis.. The Plant cell, American Society of Plant Biologists (ASPB), 2008, 20 (6), pp.1494-1503. ⟨10.1105/tpc.107.056069⟩. ⟨hal-02663189⟩
  • Sebastien Baud, Monica Santos Mendoza, Alexandra To, Erwana Harscoet, Loic Lepiniec, et al.. WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis. Plant Journal, Wiley, 2007, 50 (5), pp.825-838. ⟨10.1111/j.1365-313X.2007.03092.x⟩. ⟨hal-02664890⟩
  • Sebastien Baud, Sylvie Wuilleme, Bertrand Dubreucq, Aurélie de Almeida, Cindy Vuagnat, et al.. Function of plastidial pyruvate kinases in seeds of Arabidopsis thaliana. Plant Journal, Wiley, 2007, 52 (3), pp.405-419. ⟨10.1111/j.1365-313X.2007.03232.x⟩. ⟨hal-02661879⟩
  • Monica Santos-Mendoza, Bertrand Dubreucq, Martine Miquel, Michel Caboche, Loic Lepiniec. LEAFY COTYLEDON 2 activation is sufficient to trigger the accumulation of oil and seed specific mRNAs in Arabidopsis leaves. FEBS Letters, Wiley, 2005, 579 (21), pp.4666-4670. ⟨10.1016/j.febslet.2005.07.037⟩. ⟨hal-02682706⟩
  • A. Baudry, M.A. Heim, Bertrand Dubreucq, B. Weisshaar, Loic Lepiniec. TT2, TT8 and TTG1 synergistically specify the expression of BANYULS and proanthocyanidin biosynthesis in Arabidopsis. Plant Journal, Wiley, 2004, 39 (3), pp.366-380. ⟨10.1111/j.1365-313X.2004.02138.x⟩. ⟨hal-02683484⟩
  • Franck Samson, Véronique Brunaud, Sandrine Balzergue, Bertrand Dubreucq, Loic Lepiniec, et al.. FLAGdb/FST: a database of mapped flanking insersion sites (FSTs) of Arabidopsis thaliana T-DNA transformants. Nucleic Acids Research, Oxford University Press, 2002, 30 (1), pp.94-97. ⟨hal-02678939⟩
  • Véronique Brunaud, Sandrine Balzergue, Bertrand Dubreucq, Sebastien Aubourg, Franck Samson, et al.. T-DNA integration into Arabidopsis genome depends on sequences of pre-insertion sites. EMBO Reports, EMBO Press, 2002, 3 (12), pp.1152-1157. ⟨10.1093/embo-reports/kvf237⟩. ⟨hal-02682810⟩
  • Esther Lechner, Daoxin Xie, Sandrine Grava, Emmanuelle Pigaglio, Séverine Planchais, et al.. The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2002, 277 (51), pp.50069-50080. ⟨10.1074/jbc.M204254200⟩. ⟨hal-02682424⟩
  • Sandrine Balzergue, Bertrand Dubreucq, Sylvie Chauvin, I. Le-Clainche, F. Le Boulaire, et al.. Improved PCR-walking for large-scale isolation of plant T-DNA borders. Biotechniques, Eaton Publishing, 2001, 30 (3), 3 p. ⟨hal-02670631⟩
  • Bertrand Dubreucq, Philippe Grappin, Martine Miquel, Helen North, Christine Rochat, et al.. Approches moléculaires de la qualité et du développement des graines. Oléagineux, Corps Gras, Lipides, John Libbey Eurotext, 2001, 8 (5), pp.487-495. ⟨hal-02674355⟩
  • M. Boisson, Veronique Gomord, Corinne Audran, Nathalie Berger, Bertrand Dubreucq, et al.. Arabidopsis glucosidase I mutants reveal a critical role of N-glycan trimming in seed development. EMBO Journal, EMBO Press, 2001, 20 (5), pp.1010-1019. ⟨hal-02683628⟩
  • Bertrand Dubreucq, Nathalie Berger, E. Vincent, M. Boisson, G. Pelletier, et al.. The Arabidopsis AtPRP1 extensin-like gene is specifically expressed in endosperm during seed germination. Plant Journal, Wiley, 2000, pp.643-652. ⟨hal-02688788⟩
  • Michel Caboche, Bertrand Dubreucq, Philippe Grappin, P. Lepiniec, Nathalie Nesi. La germination vient en dormant. Biofutur, Elsevier - Cachan : Lavoisier, 1998, pp.32-35. ⟨hal-02689896⟩
  • Bertrand Dubreucq, Philippe Grappin, Michel Caboche. A new method for the identification and isolation of genes essential for Arabidopsis thaliana seed germination. Molecular and General Genetics MGG, Springer Verlag, 1996, 252, pp.42-50. ⟨hal-02692047⟩

Conference papers10 documents

  • Estelle Chaix, Bertrand Dubreucq, Abdelhak Fatihi, Dialekti Valsamou, Robert Bossy, et al.. Overview of the regulatory network of plant seed development (SeeDev) task at the BioNLP shared task 2016. BioNLP Shared Task, Aug 2016, Berlin, Germany. pp.113. ⟨hal-01455854⟩
  • Estelle Chaix, Bertrand Dubreucq, Dialekti Valsamou, Abdelhak Fatihi, Louise Deleger, et al.. Shared Task SeeDev : Extraction de régulations impliquées dans le développement de la graine d’Arabidopsis thaliana à partir de publications scientifiques. Les journées Bioinformatique de l'Inra, Mar 2016, Montpellier, France. pp.1. ⟨hal-01455855⟩
  • Estelle Chaix, Bertrand Dubreucq, Dialekti Valsamou, Abdelhak Fatihi, Robert Bossy, et al.. Information extraction challenge Gene Regulation Network in Arabidopsis thaliana (GRNA). Paris-Saclay Center for Data Science: Open Software Initiative (OSI), Oct 2015, Orsay, France. pp.14 slides, ⟨10.13140/RG.2.1.4286.6326⟩. ⟨hal-01603453⟩
  • Bertrand Dubreucq, Dialekti Valsamou, Abdelhak Fatihi, Estelle Chaix, Robert Bossy, et al.. Information extraction from articles for the elaboration of the regulatory networks involved in Arabidopsis seed development. 26th International Conference on Arabidopsis Research, Jul 2015, Paris, France. ⟨hal-01524850⟩
  • Estelle Chaix, Bertrand Dubreucq, Dialekti Valsamou, Abdelhak Fatihi, Robert Bossy, et al.. Knowledge model of regulatory networks involved in Arabidopsis seed development for information extraction and integration from text. BioCreative 5, Sep 2015, Madrid, Spain. ⟨hal-01512197⟩
  • Ghassen Trigui, Martine Miquel, Bertrand Dubreucq, Olivier David, Alain Trubuil. Analysis of factors affecting the growth of oil bodies in A. Thaliana seeds: use of ordinary least squares and quantile regression. The 10th International Workshop on Computational Systems Biology, WCSB 2013, Jun 2013, Tampere, Finland. pp.144. ⟨hal-02748214⟩
  • Ghassen Trigui, Béatrice Laroche, Martine Miquel, Bertrand Dubreucq, Alain Trubuil. The dynamics of oil bodies in A. thaliana seeds :A mathematical model of biogenesis and coalescence. ICMB 2012 - VIII International Conference on Mathematical Biology, Labo/service de l'auteur, Ville service., Apr 2012, Paris, France. pp.763-768. ⟨hal-01019421⟩
  • Colette Larre, Anne-Laure Chateigner Boutin, Brigitte Bouchet, Fabrice Petitpas, Hélène Rogniaux, et al.. Brachypodium distachyon grain: development-associated changes in morphology and storage accumulation. 1. European Brachypodium Workshop, Oct 2011, Versailles, France. ⟨hal-02809921⟩
  • Bertrand Dubreucq, B. Martin, Anne Ferlay, Philippe Pradel, Isabelle Verdier-Metz, et al.. L'alimentation des vaches est susceptible de modifier les caractéristiques sensorielles du lait. 9. Rencontres Recherches Ruminants, Dec 2002, Paris, France. ⟨hal-02761189⟩
  • Loic Lepiniec, Sandrine Balzergue, Nicole Bechtold, M. Boisson, Bertrand Dubreucq, et al.. Isolation and characterization of Arabidopsis mutants affected in seed development or germination. 9. International conference on Arabidopsis research, Jun 1998, Madison, United States. ⟨hal-02765356⟩

Books1 document

  • Bertrand Dubreucq, C. Vroemen, Loic Lepiniec, S. de Vries, Michel Caboche, et al.. Enhancer and gene-trapping in Arabidopsis thaliana, as tool to identify molecular markers of seed germination. 5 p., 1999. ⟨hal-02841906⟩

Book sections1 document

Patents1 document

  • Yannick Bellec, Bertrand Dubreucq, Jean-Denis Faure, Martine Miquel. DISPOSITIF D'EXTRACTION DE LIQUIDE DEPUIS UNE SUBSTANCE NOTAMMENT DEPUIS DES GRAINES OLEAGINEUSES. France, N° de brevet: 1461385. 2014. ⟨hal-01529245⟩

Other publications1 document

  • Estelle Chaix, Bertrand Dubreucq, Dialekti Valsamou, Abdelhak Fatihi, Louise Deleger, et al.. Annotation Guidelines BIONLP-ST 2016 SeeDev task. 2016, pp.47. ⟨hal-02795594⟩