Béatrice Roche
14
Documents
Présentation
***EDUCATION***
**2015 Ph.D in microbiology**, University of Aix-Marseille, IMM, France.
**2011 Postgraduate engineer**, Polytech School of Advanded Studies in Engineering of Luminy, Marseille, France.
**2008 Bachelor's** degree in cellular biology, University of Aix-Marseille, France
***RESEARCH EXPERIENCES***
**2019-2022 Post-doctoral researcher**, University of Basel, Biozentrum; Infection Biology, Basel; Switzerland. Director: Pr Dirk BUMANN.
**2016-2019 Post-doctoral researcher**, University of Strasbourg-CNRS, ESBS, UMR 7242, Illkirch, France. Director: Dr Isabelle SCHALK.
**2011-2015 Ph.D** in microbiology, LCB, Marseille, France. Director: Pr Frédéric BARRAS.
**2011** **Engineering internship**, Nosopharm Biotech company, Nîmes, France.
***MENTORING***
**2019-2020 Master**, Jasmin Künnecke, Biozentrum, Basel, Switzerland
**2017-2019 Ph.D student**, Anne Bonneau, ESBS, Illkirch, France. MRT fellowship.
**2017 Master**, Anne Bonneau, ESBS, Illkirch, France.
**2017** **Training** in protein purification in liquid chromatography, CNRS-ESBS, Illkirch, France.
***GRANTS/FUNDINGS***
**2018 Instruct** (PID 6435), Macromolecular crystallization, 1500 €
**2017 FRISBI** funding, Electron microscopy, 500 €
**2017 Instruct** funding (PID 2886), Electron microscopy, 1500 €
**2016-2019** **Hoffmann-La Roche** post-doctoral fellowship
**2015** **Foundation for Medical Research (FRM)**, 4th year PhD salary
**2011-2015** French Ministry of Advanced Instruction and Research, Ph.D fellowship
***MAJOR SKILLS***
**Writing: fundings and research articles (12)** including 6 first author and 3 corresponding author.
**Review Editor** in Frontiers in Microbiology – Infectious Agents and Disease (from Nov 2021)
**Molecular biology**: overlap PCR, gene cloning, construction of deletion mutant, PCR mutagenesis, RNA extraction.
**Microbiology**: *E. coli* and BSL2 pathogens (*P. aeruginosa*, *S. enterica*), 55Fe uptake assay, conjugation, antibiotic killing.
**Biochemistry**: protein production and purification (liquid chromatography), purification of membrane proteins, electrophoresis SDS-PAGE, immunodetection assays.
**Biophysics/microscopy**: flow-cytometry, phase contrast and fluorescence microscopy, negative-stain EM data processing with Relion2, EMAN2.1, Chimera.
**Animal skills**: mice handling, iv injection (FELASA number LTK4727)
***PUBLICATIONS***
1\. **Roche B** \*, Garcia-Rivera MA, Normant V, Kuhn L, Hammann P, Brönstrup M, Mislin GLA, Schalk IJ \*. A role for PchHI as the ABC transporter in iron acquisition by the siderophore pyochelin in Pseudomonas aeruginosa. *Environ Microbiol.* **2021**. doi: 10.1111/1462-2920.15811
2\. **Roche B** \*, Mislin GLA and Schalk IJ. Identification of the fatty acid coenzyme-A ligase FadD1 as an interacting partner of FptX in the Pseudomonas aeruginosa pyochelin pathway. *FEBS Lett*. **2020**; 595: 370-378.
3\. **Roche B** and Bumann D \*. Single-cell reporters for pathogen responses to antimicrobial host attacks. *Curr Opin Microbiol*. **2020**; 59:16-23.
4\. Perraud Q, Cantero P, **Roche B**, Gasser V, Normant VP, Kuhn L, Hammann P, Mislin GLA, Ehret-Sabatier L and Schalk IJ \*. Phenotypic Adaption of Pseudomonas aeruginosa by Hacking Siderophores Produced by Other Microorganisms. *Mol Cell Proteomics*. **2020**;19: 589–607.
5\. Bonneau A, **Roche B** \***,** Schalk IJ \*. Iron acquisition in Pseudomonas aeruginosa by the siderophore pyoverdine: an intricate interacting network including periplasmic and membrane proteins. *Sci Rep*. **2020**;10: 120.
6\. Paulen A, Hoegy F, **Roche B**, Schalk IJ, Mislin GLA \*. Synthesis of conjugates between oxazolidinone antibiotics and a pyochelin analogue. *Bioorg Med Chem Lett*. **2017**;27: 4867–4870.
7\. Ganne G, Brillet K, Basta B, **Roche B**, Hoegy F, Gasser V and Schalk IJ \*. Iron Release from the Siderophore Pyoverdine in Pseudomonas aeruginosa Involves Three New Actors: FpvC, FpvG, and FpvH. *ACS Chem Biol*. **2017**;12: 1056–1065.
8\. **Roche B**, Agrebi R, Huguenot A, Ollagnier de Choudens S, Barras F, Py B \*. Turning Escherichia coli into a Frataxin-Dependent Organism. *PLoS Genet*. **2015**;11: e1005134.
9\. **Roche B**, Huguenot A, Barras F, Py B \*. The iron-binding CyaY and IscX proteins assist the ISC-catalyzed Fe-S biogenesis in Escherichia coli. *Mol Microbiol*. **2015**;95: 605–623.
10\. Yan R, Konarev PV, Iannuzzi C, Adinolfi S, **Roche B,** Kelly G, et al. Ferredoxin competes with bacterial frataxin in binding to the desulfurase IscS. *J Biol Chem*. **2013**;288: 24777–24787.
11\. **Roche B**, Aussel L, Ezraty B, Mandin P, Py B, Barras F \*. Iron/sulfur proteins biogenesis in prokaryotes: formation, regulation and diversity. *Biochim Biophys Acta*. **2013**;1827: 455–469.
12\. Bordi C, Lamy M-C, Ventre I, Termine E, Hachani A, Fillet S, **Roche B**, Bleves S, et al. Regulatory RNAs and the HptB/RetS signalling pathways fine-tune Pseudomonas aeruginosa pathogenesis. *Mol Microbiol.* **2010**;76: 1427–1443.
\* = corresponding author
***SCIENTIFIC COMMUNICATIONS***
**2022&2021**-Biozentrum, Basel, Switzerland ; **2020**-LBP, Illkirch, France ; **2020-**Biozentrum, Basel, Switzerland ; **2018**-Institut Pasteur, Paris, France ; **2017**-Hoffmann-la Roche, Basel, Switzerland ; **2017**-ESBS, Illkirch, France ; **2016**-ESBS, Illkirch, France ; **2015**-University of Uppsala, Sweden, EMBO fellowship interview ; **2015**-Norwich, UK ; **2014**-MRC, UK ; **2013**- University of South Carolina, USA ; **2013**-LCB, Marseille, France.