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Badreddine Douzi

  • Career Biography

Once he obtained a diploma in Biotechnology Engineering from University of Sfax in Tunisia, Badreddine Douzi moved to France to start a scientific career in the field of microbiology. He studied biochemistry, structural biology and molecular biology at the University of Aix-Marseille and obtained his Master degree. Then he earned a PhD in biochemistry and structural Biology under the supervision of Dr Romé Voulhoux and Dr Mariella Tegoni. His thesis work was dedicated to the study of molecular mechanisms underlying the assembly and function of a bacterial nanomachine called the type II secretion system (T2SS). Fascinated by these bacterial nanomachines, he was interested during his first postdoctorat, under the supervision of Dr Christian Cambillau, by studying a different secretion system called the type VI secretion system (T6SS) dedicated to the injection of toxins into neighboring cells.  Then he moved to the University of British Columbia in the laboratory of Prof Franck Duong to study the molecular mechanisms that coordinates the translocation of colicins across the bacterial envelope. In 2015, he went back to France to join the team of Dr Romé Voulhoux as research associate to work on the T2SS, specifically on the assembly and function of the portal complex called the Secretin. After a short period on the laboratory of Dr Etienne Maisonneuve, Badreddine Douzi was appointed “Chargé de Recherche at the INRA in 2018 in the DynAMic laboratory.


  • Research interest

Working on bacterial nanomachines since 2008, Badreddine Douzi main’s interests are to understand, at molecular levels, the mechanisms underlying the traffic of molecules across the bacterial envelope. The transport of molecules is orchestrated by a specialized bacterial apparatus called secretion systems. They are large macromolecular complexes dedicated to the transport of proteins and DNA to the extracellular milieu or directly to host cells. Among the nine secretion systems identified up to date, Badreddine Douzi is currently focused on the study of the assembly and function of the conjugative type IV secretion system (T4SS) in Gram + bacteria. These nanomachines are responsible for the transfer of mobile genetic elements between bacteria and consequently play a crucial role in horizontal gene transfer. He is using complementary approaches (genetics, molecular biology, biochemistry and structural biology) to understand how these nanomachines assembles on the bacterial envelope and ensure the DNA transfer.


Journal articles29 documents

  • Christopher Dade, Badreddine Douzi, Christian Cambillau, Genevieve Ball, Romé Voulhoux, et al.. The crystal structure of CbpD clarifies substrate-specificity motifs in chitin-active lytic polysaccharide monooxygenases. Acta crystallographica Section D : Structural biology [1993-..], International Union of Crystallography, 2022, 78 (8), ⟨10.1107/S2059798322007033⟩. ⟨hal-03739502⟩
  • Julien Cappele, Abbas Mohamad Ali, Nathalie N. Leblond-Bourget, Sandrine Mathiot, Tiphaine Dhalleine, et al.. Structural and Biochemical Analysis of OrfG: The VirB8-like Component of the Conjugative Type IV Secretion System of ICESt3 From Streptococcus thermophilus. Frontiers in Molecular Biosciences, Frontiers Media, 2021, 8, ⟨10.3389/fmolb.2021.642606⟩. ⟨hal-03196531⟩
  • Cristian Escobar, Badreddine Douzi, Geneviève Ball, Brice Barbat, Sebastien Alphonse, et al.. Structural interactions define assembly adapter function of a type II secretion system pseudopilin. Structure, Elsevier (Cell Press), 2021, 29 (10), pp.1116-1127.e8. ⟨10.1016/j.str.2021.05.015⟩. ⟨hal-03367306⟩
  • Xiaomei Xu, Véronique Risoul, Deborah Byrne, Stéphanie M Champ, Badreddine Douzi, et al.. HetL, HetR and PatS form a reaction-diffusion system to control pattern formation in the cyanobacterium Nostoc PCC 7120. eLife, eLife Sciences Publication, 2020, 9, ⟨10.7554/eLife.59190⟩. ⟨hal-02917356⟩
  • Elsa Germain, Paul Guiraud, Deborah Byrne, Badreddine Douzi, Meriem Djendli, et al.. YtfK activates the stringent response by triggering the alarmone synthetase SpoT in Escherichia coli. Nature Communications, Nature Publishing Group, 2019, 10 (1), ⟨10.1038/s41467-019-13764-4⟩. ⟨hal-02446175⟩
  • Melvin G Renault, Jordi Zamarreño Beas, Badreddine Douzi, Maïalène Chabalier, Abdelrahim Zoued, et al.. The gp27-like Hub of VgrG Serves as Adaptor to Promote Hcp Tube Assembly. Journal of Molecular Biology, Elsevier, 2018, 430 (18 Pt B), pp.3143-3156. ⟨10.1016/j.jmb.2018.07.018⟩. ⟨hal-01847145⟩
  • Badreddine Douzi, Laureen Logger, Silvia Spinelli, Stephanie Blangy, Christian Cambillau, et al.. Structure–Function Analysis of the C-Terminal Domain of the Type VI Secretion TssB Tail Sheath Subunit. Journal of Molecular Biology, Elsevier, 2018, 430 (3), pp.297 - 309. ⟨10.1016/j.jmb.2017.11.015⟩. ⟨hal-01780748⟩
  • van Son Nguyen, Badreddine Douzi, Eric Durand, Alain Roussel, E. Cascales, et al.. Towards a complete structural deciphering of Type VI secretion system. Current Opinion in Structural Biology, Elsevier, 2018, 49, pp.77 - 84. ⟨10.1016/⟩. ⟨hal-01780753⟩
  • Sandra Michel-Souzy, Badreddine Douzi, Frederic Cadoret, Claire Raynaud, Loïc Quinton, et al.. Direct interactions between the secreted effector and the T2SS components GspL and GspM reveal a new effector-sensing step during type 2 secretion. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2018, 293 (50), pp.19441-19450. ⟨10.1074/jbc.RA117.001127⟩. ⟨hal-01919531⟩
  • Badreddine Douzi, Nhung Trinh, Sandra Michel-Souzy, Aline Desmyter, Genevieve Ball, et al.. Unraveling the Self-Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics. mBio, American Society for Microbiology, 2017, 8 (5), ⟨10.1128/mBio.01185-17⟩. ⟨hal-01744494⟩
  • Badreddine Douzi, Yannick R Brunet, Silvia Spinelli, Valentine Lensi, Stephanie Blangy, et al.. Structure and specificity of the Type VI secretion system ClpV-TssC interaction in enteroaggregative Escherichia coli. Scientific Reports, Nature Publishing Group, 2016, 6 (1), ⟨10.1038/srep34405⟩. ⟨hal-01780690⟩
  • Abdelrahim Zoued, Eric Durand, Yannick R Brunet, Silvia Spinelli, Badreddine Douzi, et al.. Priming and polymerization of a bacterial contractile tail structure. 1" 2" 17". Nature, Nature Publishing Group, 2016, 531 (7592), pp.59 - 63. ⟨10.1038/nature17182⟩. ⟨hal-01778575⟩
  • Abdelrahim Zoued, Chloé J Cassaro, Eric Durand, Badreddine Douzi, Alexandre España, et al.. Structure–Function Analysis of the TssL Cytoplasmic Domain Reveals a New Interaction between the Type VI Secretion Baseplate and Membrane Complexes. Journal of Molecular Biology, Elsevier, 2016, 428 (22), pp.4413 - 4423. ⟨10.1016/j.jmb.2016.08.030⟩. ⟨hal-01780163⟩
  • Yannick R Brunet, Abdelrahim Zoued, Frédéric Boyer, Badreddine Douzi, E. Cascales. The Type VI Secretion TssEFGK-VgrG Phage-Like Baseplate Is Recruited to the TssJLM Membrane Complex via Multiple Contacts and Serves As Assembly Platform for Tail Tube/Sheath Polymerization. PLoS Genetics, Public Library of Science, 2015, 11 (10), pp.e1005545. ⟨10.1371/journal.pgen.1005545⟩. ⟨hal-01458191⟩
  • van Son Nguyen, Laureen Logger, Silvia Spinelli, Aline Desmyter, Thi Thu Hang Le, et al.. Inhibition of Type VI Secretion by an Anti-TssM Llama Nanobody. PLoS ONE, Public Library of Science, 2015, 10 (e0122187), pp.1-14. ⟨hal-01217205⟩
  • Badreddine Douzi, Silvia Spinelli, Stéphanie Blangy, Alain Roussel, Eric Durand, et al.. Crystal Structure and Self-Interaction of the Type VI Secretion Tail-Tube Protein from Enteroaggregative Escherichia coli. PLoS ONE, Public Library of Science, 2014, 9 (2), pp.e86918. ⟨10.1371/journal.pone.0086918⟩. ⟨hal-02091448⟩
  • Frederic Cadoret, Geneviève Ball, Badreddine Douzi, Romé Voulhoux. Txc, a new type II secretion system of Pseudomonas aeruginosa strain PA7, is regulated by the TtsS/TtsR two-component system and directs specific secretion of the CbpE chitin-binding protein.. Journal of Bacteriology, American Society for Microbiology, 2014, 196 (13), pp.2376--86. ⟨10.1128/JB.01563-14⟩. ⟨hal-01458218⟩
  • Abdelrahim Zoued, Yannick R Brunet, Eric Durand, Marie-Stéphanie Aschtgen, Laureen Logger, et al.. Architecture and assembly of the Type VI secretion system.. Biochim. Biophys. Acta, 2014, 1843 (8), pp.1664--73. ⟨10.1016/j.bbamcr.2014.03.018⟩. ⟨hal-01458220⟩
  • Abdelrahim Zoued, Eric Durand, Cecilia C. Bebeacua, Yannick R Brunet, Badreddine Douzi, et al.. TssK Is a Trimeric Cytoplasmic Protein Interacting with Components of Both Phage-like and Membrane Anchoring Complexes of the Type VI Secretion System. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2013, 288 (38), pp.27031-27041. ⟨10.1074/jbc.M113.499772⟩. ⟨hal-02091435⟩
  • Xiang Y. Zhang, Yannick R Brunet, Laureen Logger, Badreddine Douzi, Christian Cambillau, et al.. Dissection of the TssB-TssC Interface during Type VI Secretion Sheath Complex Formation. PLoS ONE, Public Library of Science, 2013, 8 (11), pp.e81074. ⟨10.1371/journal.pone.0081074⟩. ⟨hal-02091436⟩
  • Hervé Nozach, Carole Fruchart-Gaillard, François Fenaille, Fabrice Beau, Oscar Pereira Ramos, et al.. High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli. Microbial Cell Factories, BioMed Central, 2013, 12, ⟨10.1186/1475-2859-12-37⟩. ⟨cea-02100032⟩
  • Badreddine Douzi, Alain Filloux, Romé Voulhoux. On the path to uncover the bacterial type II secretion system.. Philos. Trans. R. Soc. Lond., B, Biol. Sci., 2012, 367 (1592), pp.1059--72. ⟨10.1098/rstb.2011.0204⟩. ⟨hal-01458255⟩
  • Catarina Felisberto-Rodrigues, Eric Durand, Marie-Stéphanie Aschtgen, Stephanie Blangy, Miguel Ortiz-Lombardia, et al.. Towards a structural comprehension of bacterial type VI secretion systems: characterization of the TssJ-TssM complex of an Escherichia coli pathovar. PLoS Pathogens, Public Library of Science, 2011, 7 (11), pp.e1002386. ⟨10.1371/journal.ppat.1002386⟩. ⟨hal-01458272⟩
  • Laura P Franz, Badreddine Douzi, Eric Durand, David H Dyer, Romé Voulhoux, et al.. Structure of the minor pseudopilin XcpW from the Pseudomonas aeruginosa type II secretion system.. Acta Crystallogr. D Biol. Crystallogr., 2011, 67 (Pt 2), pp.124--30. ⟨10.1107/S0907444910051954⟩. ⟨hal-01458266⟩
  • Badreddine Douzi, Geneviève Ball, Christian Cambillau, Mariella Tegoni, Romé Voulhoux. Deciphering the Xcp Pseudomonas aeruginosa type II secretion machinery through multiple interactions with substrates.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2011, 286 (47), pp.40792--801. ⟨10.1074/jbc.M111.294843⟩. ⟨hal-01458270⟩
  • E. Durand, S. Alphonse, Céline Brochier-Armanet, G. Ball, Badreddine Douzi, et al.. The Assembly Mode of the Pseudopilus: a hallmark to distinguish a novel secretion system subtype. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2011, 286 (27), pp.24407-24416. ⟨10.1074/jbc.M111.234278⟩. ⟨hal-02299276⟩
  • Cathy Poillot, Kaouthar Dridi, Hicham Bichraoui, Julien Pêcher, Sébastien Alphonse, et al.. D-Maurocalcine, a pharmacologically inert efficient cell-penetrating peptide analogue.: Cell Penetrating D-Maurocalcine Peptide. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2010, 285 (44), pp.34168-80. ⟨10.1074/jbc.M110.104919⟩. ⟨inserm-00587800⟩
  • S. Alphonse, E. Durand, Badreddine Douzi, B. Waegele, H. Darbon, et al.. Structure of the Pseudomonas aeruginosa XcpT pseudopilin, a major component of the type II secretion system. Journal of Structural Biology, Elsevier, 2010, 169 (1), pp.75-80. ⟨10.1016/j.jsb.2009.09.003⟩. ⟨hal-02667520⟩
  • Badreddine Douzi, E. Durand, C. Bernard, S. Alphonse, C. Cambillau, et al.. The XcpV/GspI pseudopilin has a central role in the assembly of a quaternary complex within the T2SS pseudopilus. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2009, 284 (50), pp.34580-34589. ⟨10.1074/jbc.M109.042366⟩. ⟨hal-02666049⟩

Conference papers2 documents

  • Badreddine Douzi. Nanobodies to investigate the architecture and dynamics of the Type II secretion nanomachine. Third FRISBI annual user meeting, Nov 2017, paris, France. ⟨hal-02791428⟩
  • Badreddine Douzi. Deciphering the Type II secretion system dynamics and function. EMBO-Clostridial Secretion, Sep 2012, marseille, France. ⟨hal-02750355⟩

Poster communications4 documents

  • Badreddine Douzi, Romé Voulhoux, - Nhung. T. T. Trinh, - Sandra Michel-Souzy, Aline Desmyter, et al.. Self-assembly of XcpQ secretin N domain into hexamer of dimers provides new molecular insights into architecture and dynamics of widespread outer membrane channels. New Approaches and Concepts in Microbiology, Jun 2017, Heidelberg, Germany. 2017. ⟨hal-02737898⟩
  • Badreddine Douzi, Romé Voulhoux, Eric Durand, Loïc Quinton, Edwin de Pauw, et al.. Minor pseudopilins of the type II secretion system in P. aeruginosa: placing pieces in a large puzzle. ASM Conference on Pseudomonas 2015, Sep 2015, Washington, United States. 2015. ⟨hal-02743889⟩
  • Badreddine Douzi, - Sandra Michel, Frédéric Cadoret, Geneviève Ball, Bérengère Ize, et al.. Towards a better understanding of the bacterial type II secretion pathway. SGM ANNUAL CONFERENCE, Apr 2014, Liverpool, United Kingdom. 2014. ⟨hal-02744249⟩
  • Badreddine Douzi, Sébastien Alphonse, Herve Darbon, Alain Filloux, Christian Cambillau, et al.. The XcpV/GspI pseudopilin has a central role in the assembly of a quaternary complex within the T2SS pseudopilus. Colloque de l'école doctorale Aix-Marseille, Jun 2009, Marseille, France. 1p, 2009. ⟨hal-02824819⟩

Book sections1 document

Theses1 document

  • Badreddine Douzi. La machinerie de sécrétion de type II Xcp de pseudomonas aeruginosa : Relations structure-fonction et interactome. Sciences du Vivant [q-bio]. Université de Provence, 2011. Français. ⟨tel-02806009⟩

Reports1 document

  • Badreddine Douzi. Assessment of Research and Innovation on Food Systems by European member States: Policy and Funding Analysis. [Other] Ruropean Commision. 2018. ⟨hal-02947601⟩

Videos2 documents

  • Badreddine Douzi, Sophie Payot, Nicolas Soler. Limiter l'antibiorésistance, un enjeu du 21e siècle. 2020. ⟨hal-03228358⟩
  • Claude Didierjean, Badreddine Douzi. Laboratoire Dynamic . Une collaboration avec le CRM2, laboratoire de Cristallographie, Résonance Magnétique et Modélisations. 2020. ⟨hal-03367957⟩