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Patrick Chames

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Affiliations actuelles
  • 441569
  • 303623
  • 179570
  • 301325
  • 198056
Identifiants chercheurs
Contact

Présentation

The main interest of our lab is to use antibody engineering solutions to generate innovative molecules able to efficiently modulate the anti-tumor immune response through the recruitment and activation of immune cells using multispecific cell engagers or multispecific immune checkpoint inhibitors, or through the vectorization of radionuclides and nanoparticles. Our team has developed a solid expertise in the generation and use of nanobodies, corresponding to the variable domain of antibodies naturally devoid of light chain found in camelids, for cancer therapy and diagnostic using in vitro selection methods. We are exploring the possibilities offered by this technology for the development of innovative cancer imaging and therapy approaches.

Domaines de recherche

Cancer Immunothérapie

Compétences

Publications

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Specific Targeting of Mesothelin-Expressing Malignant Cells Using Nanobody-Functionalized Magneto-Fluorescent Nanoassemblies

 Tina Briolay , Judith Fresquet , Damien Meyer , Brigitte Kerfelec , Patrick Chames
International Journal of Nanomedicine, 2024, 19, pp.633 - 650. ⟨10.2147/ijn.s435787⟩
Article dans une revue inserm-04465814v1
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Trispecific T-cell engagers for dual tumor-targeting of colorectal cancer

 Antonio Tapia-Galisteo , Íñigo Sánchez Rodríguez , Oscar Aguilar-Sopeña , Seandean Lykke Harwood , Javier Narbona
OncoImmunology, 2022, 11, ⟨10.1080/2162402x.2022.2034355⟩
Article dans une revue hal-03745498v1
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Nanobody-based sensors reveal a high proportion of mGlu heterodimers in the brain

 Jiyong Meng , Chanjuan Xu , Pierre-André Lafon , Salomé Roux , Michaël Mathieu
Nature Chemical Biology, 2022, 18, pp.894-903. ⟨10.1038/s41589-022-01050-2⟩
Article dans une revue hal-03699580v1
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A nanobody activating metabotropic glutamate receptor 4 discriminates between homo- and heterodimers

 Jordi Haubrich , Joan Font , Robert Quast , Anne Goupil-Lamy , Pauline Scholler
Proceedings of the National Academy of Sciences of the United States of America, 2021, 118 (33), pp.e2105848118. ⟨10.1073/pnas.2105848118⟩
Article dans une revue hal-03322348v1
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A Novel Anti-Kv10.1 Nanobody Fused to Single-Chain TRAIL Enhances Apoptosis Induction in Cancer Cells

 Franziska Hartung , Thomas Krüwel , Xiaoyi Shi , Klaus Pfizenmaier , Roland Kontermann
Frontiers in Pharmacology, 2020, 11, ⟨10.3389/fphar.2020.00686⟩
Article dans une revue hal-03017218v1
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Anti-NKG2D single domain-based antibodies for the modulation of anti-tumor immune response

 Adeline Raynaud , Klervi Desrumeaux , Laurent Vidard , Elise Termine , Daniel Baty
OncoImmunology, 2020, 10, ⟨10.1080/2162402x.2020.1854529⟩
Article dans une revue hal-03371613v1
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Special Issue: Nanobody

 Patrick Chames , Ulrich Rothbauer
Antibodies, 2020, 9 (1), pp.6. ⟨10.3390/antib9010006⟩
Article dans une revue hal-03017191v1
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Nanobody Engineering: Toward Next Generation Immunotherapies and Immunoimaging of Cancer

 Timothée Chanier , Patrick Chames
Antibodies, 2019, 8 (1), pp.13. ⟨10.3390/antib8010013⟩
Article dans une revue hal-02002577v1
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Nanobody-CD16 Catch Bond Reveals NK Cell Mechanosensitivity

 Cristina González , Patrick Chames , Brigitte Kerfelec , Daniel Baty , Philippe Robert
Biophysical Journal, 2019, 116 (8), pp.1516-1526. ⟨10.1016/j.bpj.2019.03.012⟩
Article dans une revue hal-02323625v1
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A Bispecific Antibody-Based Approach for Targeting Mesothelin in Triple Negative Breast Cancer

 Joanie del Bano , Rémy Florès-Florès , Emmanuelle Josselin , Armelle Goubard , Laëtitia Ganier
Frontiers in Immunology, 2019, 10, pp.1593. ⟨10.3389/fimmu.2019.01593⟩
Article dans une revue hal-02520930v1

Nanophotonic tools based on the conjugates of nanoparticles with the single-domain antibodies for multi-photon micrometastases detection and ultrasensitive biochemical assays

 Alyona Sukhanova , Fernanda Ramos-Gomes , Patrick Chames , Daniel Baty , Frauke Alves
Proceedings of SPIE, the International Society for Optical Engineering, 2019, pp.112072A. ⟨10.1117/12.2530233⟩
Article dans une revue hal-03007502v1
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Les anticorps et scaffold bispécifiques, des médicaments innovants en oncologie impliquant le ciblage des cellules immunitaires

 Patrick Chames , Thierry Wurch
Médecine/Sciences, 2019, 35 (12), pp.1072-1082. ⟨10.1051/medsci/2019242⟩
Article dans une revue hal-03017227v1

Advanced Nanotools for Imaging of Solid Tumors and Circulating and Disseminated Cancer Cells

 D. Baty , I. Nabiev , A. Sukhanova , F. Ramos-Gomes , F. Alves
Optics and Spectroscopy, 2018, 125 (5), pp.703-707. ⟨10.1134/S0030400X18110309⟩
Article dans une revue hal-02143643v1
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Single- and two-photon imaging of human micrometastases and disseminated tumour cells with conjugates of nanobodies and quantum dots

 Fernanda Ramos-Gomes , Julia Bode , Alyona Sukhanova , Svetlana V Bozrova , Mara Saccomano
Scientific Reports, 2018, 8 (1), pp.4595. ⟨10.1038/s41598-018-22973-8⟩
Article dans une revue hal-01765351v1
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Allosteric nanobodies uncover a role of hippocampal mGlu2 receptor homodimers in contextual fear consolidation

 Pauline Scholler , Damien Nevoltris , Dimitri de Bundel , Simon Bossi , David Moreno-Delgado
Nature Communications, 2017, 8 (1), pp.1967. ⟨10.1038/s41467-017-01489-1⟩
Article dans une revue hal-01672805v2

Nanosized Fluorescent Diagnostic Probes Consisting of Single-domain Antibodies Conjugated with Quantum Dots

 Alyona Sukhanova , Klervi Even-Desrumeaux , Jean-Marc Millot , Patrick Chames , Daniel Baty
Materials Today: Proceedings, 2016, 3 (2), pp.518-522. ⟨10.1016/j.matpr.2016.01.083⟩
Article dans une revue hal-03112307v1

In vivo detection of small tumour lesions by multi-pinhole SPECT applying a (99m)Tc-labelled nanobody targeting the Epidermal Growth Factor Receptor.

 Thomas Krüwel , Damien Nevoltris , Julia Bode , Christian Dullin , Daniel Baty
Scientific Reports, 2016, 6, pp.21834
Article dans une revue hal-01450793v1
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V1/V2 Neutralizing Epitope is Conserved in Divergent Non-M Groups of HIV-1

 Marion Morgand , Mélanie Bouvin-Pley , Jean-Christophe Plantier , Moreau Alain , Elodie Alessandri
Journal of Acquired Immune Deficiency Syndromes - JAIDS, 2016, 71 (3), pp.237-245. ⟨10.1097/QAI.0000000000000854⟩
Article dans une revue hal-01314296v1

Multiphoton Imaging of Tumor Biomarkers in situ Using Single-domain Antibodies Conjugated with Quantum Dots in a Set Orientation

 Alyona Sukhanova , Hilal Hafian , Marc Turini , Patrick Chames , Daniel Baty
Materials Today: Proceedings, 2016, 3 (2), pp.523-526. ⟨10.1016/j.matpr.2016.01.084⟩
Article dans une revue hal-03112311v1
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Taking up Cancer Immunotherapy Challenges: Bispecific Antibodies, the Path Forward?

 Joanie del Bano , Patrick Chames , Daniel Baty , Brigitte Kerfelec
Antibodies, 2016, 5 (1), ⟨10.3390/antib5010001⟩
Article dans une revue hal-01450799v1

Detection of carcinoembryonic antigen using single-domain or full-size antibodies stained with quantum dot conjugates.

 Gilles Rousserie , Regina Grinevich , Kristina Brazhnik , Klervi Even-Desrumeaux , Brigitte Reveil
Analytical Biochemistry, 2015, 478, pp.26-32. ⟨10.1016/j.ab.2015.02.029⟩
Article dans une revue hal-01450782v1
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Oriented Conjugates of Single-domain Antibodies and Fluorescent Quantum Dots for Highly Sensitive Detection of Tumor-associated Biomarkers in Cells and Tissues

 Regina Bilan , Kristina Brazhnik , Patrick Chames , Daniel Baty , Igor Nabiev
Physics Procedia, 2015, 73, pp.228-234. ⟨10.1016/j.phpro.2015.09.162⟩
Article dans une revue hal-03112376v1

Conformational nanobodies reveal tethered epidermal growth factor receptor involved in EGFR/ErbB2 predimers.

 Damien Nevoltris , Benjamin Lombard , Elodie Dupuis , Gérard Mathis , Patrick Chames
ACS Nano, 2015, 9 (2), pp.1388-99. ⟨10.1021/nn505752u⟩
Article dans une revue hal-01450765v1

Anti-Mesothelin Nanobodies for Both Conventional and Nanoparticle-Based Biomedical Applications.

 Andrew M Prantner , Marc Turini , Brigitte Kerfelec , Shree Joshi , Daniel Baty
Journal of Biomedical Nanotechnology, 2015, pp.1201-12
Article dans une revue hal-01450786v1

Highly Sensitive Single Domain Antibody–Quantum Dot Conjugates for Detection of HER2 Biomarker in Lung and Breast Cancer Cells

 Tatsiana Rakovich , Omar Mahfoud , Bashir Mohamed , Adriele Prina-Mello , Kieran Crosbie-Staunton
ACS Nano, 2014, 8 (6), pp.5682-5695. ⟨10.1021/nn500212h⟩
Article dans une revue hal-03136167v1
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A FcγRIII-engaging bispecific antibody expands the range of HER2-expressing breast tumors eligible to antibody therapy

 Marc Turini , Patrick Chames , Pierre Bruhns , Daniel Baty , Brigitte Kerfelec
Oncotarget, 2014, 5 (14), ⟨10.18632/oncotarget.2093⟩
Article dans une revue hal-02115462v1
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Masked Selection: A Straightforward and Flexible Approach for the Selection of Binders Against Specific Epitopes and Differentially Expressed Proteins by Phage Display

 Klervi Even-Desrumeaux , Damien Nevoltris , Marie Noelle Lavaut , Karima Alim , Jean-Paul Borg
Molecular and Cellular Proteomics, 2014, 13 (2), pp.653-665. ⟨10.1074/mcp.O112.025486⟩
Article dans une revue hal-02115444v1

Multiphoton imaging of tumor biomarkers with conjugates of single-domain antibodies and quantum dots

 Hilal Hafian , Alyona Sukhanova , Marc Turini , Patrick Chames , Daniel Baty
Nanomedicine: Nanotechnology, Biology and Medicine, 2014, 10 (8), pp.1701-1709. ⟨10.1016/j.nano.2014.05.014⟩
Article dans une revue hal-03136281v1
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Selection of Intracellular Single-Domain Antibodies Targeting the HIV-1 Vpr Protein by Cytoplasmic Yeast Two-Hybrid System

 Julie Matz , Cécile Hérate , Jérôme Bouchet , Nelson Dusetti , Odile Gayet
PLoS ONE, 2014, 9 (12), pp.e113729. ⟨10.1371/journal.pone.0113729⟩
Article dans une revue inserm-01093997v1

Single-Domain Antibody-Based and Linker-Free Bispecific Antibodies Targeting Fc RIII Induce Potent Antitumor Activity without Recruiting Regulatory T Cells

 C. Rozan , A. Cornillon , C. Petiard , M. Chartier , G. Behar
Molecular Cancer Therapeutics, 2013, 12 (8), pp.1481-1491. ⟨10.1158/1535-7163.MCT-12-1012⟩
Article dans une revue hal-02115468v1
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Single-domain antibody-based and linker-free bispecific antibodies targeting FcγRIII induce potent antitumor activity without recruiting regulatory T cells.

 Caroline Rozan , Amélie Cornillon , Corinne Petiard , M. Chartier , G. Behar
Molecular Cancer Therapeutics, 2013, 12 (8), pp.1481-1491. ⟨10.1158/1535-7163.MCT-12-1012⟩
Article dans une revue hal-02454959v1

Heavy Chain-Only IgG2b Llama Antibody Effects Near-Pan HIV-1 Neutralization by Recognizing a CD4-Induced Epitope That Includes Elements of Coreceptor- and CD4-Binding Sites

 P. Acharya , T. Luongo , I. Georgiev , J. Matz , S. Schmidt
Journal of Virology, 2013, 87 (18), pp.10173-10181. ⟨10.1128/JVI.01332-13⟩
Article dans une revue hal-02530765v1

Oriented conjugates of single-domain antibodies and quantum dots: toward a new generation of ultrasmall diagnostic nanoprobes

 Alyona Sukhanova , Klervi Even-Desrumeaux , Aymric Kisserli , Thierry Tabary , Brigitte Reveil
Nanomedicine: Nanotechnology, Biology and Medicine, 2012, 8 (4), pp.516-525. ⟨10.1016/j.nano.2011.07.007⟩
Article dans une revue hal-03184846v1
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Structural definition of a novel CD4-induced epitope that is targeted by a single-headed immunoglobulin to effect broad and potent HIV neutralization

 Priyamvada Acharya , Timothy S. Luongo , Julie Matz , Stephen D. Schmidt , Gwo-Yu Chuang
Retrovirology, 2012, 9 (Suppl 2), pp.P346
Article dans une revue inserm-00731776v1

Oriented conjugates of monoclonal and single-domain antibodies with quantum dots for flow cytometry and immunohistochemistry diagnostic applications

 Alyona Sukhanova , Klervi Even-Desrumeaux , Jean-Marc Millot , Patrick Chames , Daniel Baty
Proceedings of SPIE, the International Society for Optical Engineering, 2012, Colloidal Nanocrystals for Biomedical Applications VII, 8232, pp.82320T. ⟨10.1117/12.905896⟩
Article dans une revue hal-03184792v1
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Straightforward selection of broadly neutralizing single-domain antibodies targeting the conserved CD4 and co-receptor binding sites of HIV-1 gp120

 Julie Matz , Pacal Kessler , Jérome Bouchet , Olivier Combes , Daniel Baty
Retrovirology, 2012, 9 (Suppl 2), pp.P214
Article dans une revue inserm-00731772v1

Straightforward Selection of Broadly Neutralizing Single-Domain Antibodies Targeting the Conserved CD4 and Coreceptor Binding Sites of HIV-1 gp120

 J. Matz , P. Kessler , J. Bouchet , O. Combes , O. Ramos
Journal of Virology, 2012, 87 (2), pp.1137-1149. ⟨10.1128/JVI.00461-12⟩
Article dans une revue cea-02530610v1
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Single-domain antibodies: a versatile and rich source of binders for breast cancer diagnostic approaches.

 Klervi Even-Desrumeaux , Patrick Fourquet , Véronique Secq , Daniel Baty , Patrick Chames
Molecular BioSystems, 2012, 8 (9), pp.2385-2394. ⟨10.1039/c2mb25063b⟩
Article dans une revue inserm-00722930v1

Bi-photon imaging and diagnostics using ultra-small diagnostic probes engineered from semiconductor nanocrystals and single-domain antibodies

 Hilal Hafian , Alyona Sukhanova , Patrick Chames , Daniel Baty , Michel Pluot
Proceedings of SPIE, the International Society for Optical Engineering, 2012, Nanosystems in Engineering and Medicine, 8548, pp.85480R. ⟨10.1117/12.960493⟩
Article dans une revue hal-03177842v1
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Therapeutic antibodies for the treatment of pancreatic cancer.

 Patrick Chames , Brigitte Kerfelec , Daniel Baty
TheScientificWorldJournal, 2010, 10, pp.1107-20. ⟨10.1100/tsw.2010.103⟩
Article dans une revue inserm-00497886v1
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Therapeutic Antibodies for the Treatment of Pancreatic Cancer

 Patrick Chames , Brigitte Kerfelec , Daniel Baty
The Scientific World Journal, 2010, 10, pp.1107-1120. ⟨10.1100/tsw.2010.103⟩
Article dans une revue hal-02115485v1
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Strong and oriented immobilization of single domain antibodies from crude bacterial lysates for high-throughput compatible cost-effective antibody array generation.

 Klervi Even-Desrumeaux , Daniel Baty , Patrick Chames
Molecular BioSystems, 2010, 6 (11), pp.2241-8. ⟨10.1039/c005279e⟩
Article dans une revue inserm-00521371v1

Semiconductor quantum dots for multiplexed bio-detection on solid-state microarrays.

 G. Rousserie , A. Sukhanova , K. Even-Desrumeaux , F. Fleury , P. Chames
Critical Reviews in Oncology/Hematology, 2009, 00 (00), pp.00
Article dans une revue hal-00415311v1
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Bispecific antibodies for cancer therapy: the light at the end of the tunnel?

 Patrick Chames , Daniel Baty
mAbs, 2009, 1 (6), pp.539-47
Article dans une revue inserm-00448211v1

Llama single-domain antibodies directed against nonconventional epitopes of tumor-associated carcinoembryonic antigen absent from nonspecific cross-reacting antigen.

 Ghislaine Behar , Patrick Chames , Isabelle Teulon , Amélie Cornillon , Faisal Alshoukr
FEBS Journal, 2009, 276 (14), pp.3881-93. ⟨10.1111/j.1742-4658.2009.07101.x⟩
Article dans une revue hal-00420547v1

Llama single-domain antibodies directed against nonconventional epitopes of tumor-associated carcinoembryonic antigen absent from nonspecific cross-reacting antigen.

 G. Behar , P. Chames , I. Teulon , A. Cornillon , F. Alshoukr
FEBS Journal, 2009, june (00), pp.00
Article dans une revue hal-00414287v1
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[The future of antibody fragments, made of a single immunoglobulin domain.]

 Patrick Chames , Daniel Baty
Médecine/Sciences, 2009, 25 (12), pp.1159-62
Article dans une revue inserm-00443810v1

Isolation and characterization of anti-Fc{gamma}RIII (CD16) llama single-domain antibodies that activate natural killer cells.

 Ghislaine Behar , Sophie Sibéril , Agnès Groulet , Patrick Chames , Martine Pugnière
Protein Engineering, Design and Selection, 2008, 21 (1), pp.1-10. ⟨10.1093/protein/gzm064⟩
Article dans une revue hal-00258940v1
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Isolation and characterization of anti-FcgRIII (CD16) llama single-domain antibodies that activate natural killer cells

 Ghislaine Béhar , Sophie Sibéril , Agnès Groulet , Patrick Chames , Martine Pugnière
Protein Engineering, Design and Selection, 2007, 21 (1), pp.1-10. ⟨10.1093/protein/gzm064⟩
Article dans une revue hal-02454965v1

Intracellular Immunization of Prokaryotic Cells against a Bacteriotoxin

 Patrick Chames , Jacques Fieschi , Daniel Baty , Denis Duché
Journal of Bacteriology, 1998, 180 (3), pp.514-518. ⟨10.1128/JB.180.3.514-518.1998⟩
Article dans une revue hal-03269515v1

Nanophotonic tools based on the conjugates of nanoparticles with the single-domain antibodies for multi-photon micrometastases detection and ultrasensitive biochemical assays

 Alyona Sukhanova , Fernanda Ramos-Gomes , Patrick Chames , Daniel Baty , Frauke Alves
IV International Conference on Applications of Optics and Photonics, May 2019, Lisbon, France. pp.186, ⟨10.1117/12.2530233⟩
Communication dans un congrès hal-03017208v1

Multiphoton imaging of tumor biomarkers in situ using highly oriented conjugates of single-domain antibodies and quantum dots

 Alyona Sukhanova , Hilal Hafian , Michel Pluot , Jacques Cohen , Jean-Marc Millot
2015 International Conference on Biomedical Engineering and Computational Technologies (SIBIRCON), Oct 2015, Novosibirsk, France. pp.119-121, ⟨10.1109/SIBIRCON.2015.7361864⟩
Communication dans un congrès hal-03112342v1
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Engineering of ultra-small diagnostic nanoprobes through oriented conjugation of single-domain antibodies and quantum dots

 Igor Nabiev , Alyona Sukhanova , Klervi Even-Desrumeaux , Patrick Chames , Daniel Baty
2021
Pré-publication, Document de travail hal-03182577v1

Multiphoton Deep-Tissue Imaging of Micrometastases and Disseminated Cancer Cells Using Conjugates of Quantum Dots and Single-Domain Antibodies

 Alyona Sukhanova , Fernanda Ramos-Gomes , Patrick Chames , Pavel Sokolov , Daniel Baty
Methods in Molecular Biology, 2350, Springer US, pp.105-123, 2021, Multiplexed Imaging, ⟨10.1007/978-1-0716-1593-5_8⟩
Chapitre d'ouvrage hal-03500875v1

Phage Display and Selections on Purified Antigens

 Magali Colazet , Patrick Chames
Methods in Molecular Biology, 1827, pp.165-178, 2018, ⟨10.1007/978-1-4939-8648-4_9⟩
Chapitre d'ouvrage hal-02146005v1